LOCUS       sequence-459560-        9669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NIT-9 with CNX1 linkage region in N. crassa at the 
            his-3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9669)
  TITLE     Convergent Evolution Links Molybdenum Insertase Domains with 
            Organism-Specific Sequences.
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCCG_nit9fl_CNX1_linker
FEATURES             Location/Qualifiers
     source          1..9669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4979..4995
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4979..4995
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6948..6964)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6949..6965)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(join(9668..9669,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(9669,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgaggccta gcggtcccag ctccccgtaa
     2761 ccacgcagcc ccagcgaccg aggtcatcat gacactatga cgacaatctg gcgggagact
     2821 tgggaaaatc gatgacgggc acctaccttg ggaacaatag agcaagtcca gtcaacgccc
     2881 tagggaaggg ggaaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat acgactcact
     4021 atagggcgaa ttggagctcc accgcggtgg cggccgctag aaggagcagt ccatctgcgt
     4081 gaatcacgag agaatcagct actttgaatc gatggatgca gctaccagaa gtcactcagt
     4141 tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg gacgagaccg
     4201 acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg cggccttcgg
     4261 taacagaaaa gcacacagac agcgattgtg acatggattc gggcaaagca ttggtggtcg
     4321 caccaggtca tcctgagtgt gcagtggctg ctattcagat ttcatctaac tgcgggagag
     4381 gggttcaaag gggcgtgacg tcacagacaa cgggtgaagg acgaagattg cctcacttct
     4441 ttgctagcaa tttgcctgca aagaaggcgc acatgtcaag caaacaactg ggaaaccact
     4501 attgaatacc cacaatgcaa agctcggaag gtacgtcttg attgcagtgt gtcgagtgtc
     4561 aaaaaagaag caagtgttca tgcaagccaa aattggcacc tcctccactt ctccgagtgc
     4621 cccacccgaa cctccaggcg agatggccgg aacataccat ccgcgttggg attatgacgt
     4681 atctccttct tcttcacatg attccatccc gttgttgctt gtttgcgagc tgtgacggga
     4741 gatcgtagat gccacttagg gccaggcagg cagtgcaggc agccaggaac acaagcttcc
     4801 aacttggtca tctcgattgc cgattaaggg aaccaaatgc ctatataaga ctgtcctccc
     4861 acctccccaa taccattctt ttcttcttcc atcatcagcc aacaaagcaa tcacatcttc
     4921 actacttcaa atcaacacaa cactcaaacc actttcacaa cccctcacat caaccaaatc
     4981 tagaactagt ggatccccgg gttaattaac atgtcctcaa gtactactcc agcagcgctc
     5041 aaagcagcca tccttatcgt ttcaacgaca gcagctcaag accccgcaac ggactcggcc
     5101 gacgtcactc tccgccaggt cttcgatgaa caacaagccc aatgggaggt ccatgaatcc
     5161 aaaatcgttc ccgacgatgt cgtgcaaatc cagaagcaaa tcatccactg gacaaaccaa
     5221 gccaccgccg caagtggcga caacaacaac aacaacaaca ccaacaccat caacctcatc
     5281 gtaaccacag gcggcaccgg cttcgccgtg tccgaccaaa ccaccgaagc cgtctcggct
     5341 ctcctacaca agaatgctcc tggcctggtc cacggcatgc tggcggcttc gctgcaagtg
     5401 accccctttg ccatgatgtc acgacctgtg gctggcgtca ggaacggcac cgttatcgtt
     5461 gcgctgcctg ggtcccccaa gggcgcgaag gagaatctgc aagcagtgat caaaatgctg
     5521 ccccacgctt gcatgcaggc tgctggtgct aactctagaa ttcggaaaga aaagaagtac
     5581 gatgaagtac ctatgctctc tgtctccgaa gcgctctccc aaatagcgca acacacgccg
     5641 atgccgcaaa tcgtcacctc caaagtcgat agctcgctca tcggcgccgt tctggcgcag
     5701 gacgtttcgg cgcgcgaaaa tgtgcccgct tttcgcgcta gcatcgtcga cggctacgcc
     5761 gtagtcgtgc cccaagacgg gaacatgcgc ggcgtgttcc ccgtcgtgtc ggtttcgcat
     5821 gccgctcctg gagatgggga aaaaatgccg acgctcaaag agggcgagat tgcccggatc
     5881 accacgggtg cacccctgcc tccgggtgcg acatcggtta ttatggtgga ggatacggtg
     5941 ttggtgtcga cgacgtcatc caagacggag gatgcggagg atggaaaggg agtggaggag
     6001 gagaaggagg tggagatttt agcagctgat aaagttcgcg aaggcgaaaa tgttagggag
     6061 gtgggaagtg atatcaagca ggggcaactg attctaggca agggagatca ggtgtctgct
     6121 gtggggggtg agattggtct gttggccgcc gtgggtgtgg gtgaggtgca ggtgtaccga
     6181 cgtcctgttg ttggcgttct atctacgggt gatgagattg tggaccatga tcgcccggga
     6241 gacttgcggc tgggcgaggt gcgcgatacg aaccggccaa ctctcatttc ggcggcgaga
     6301 gattgggggt atgaggtgat tgatcttgga gtggcgcggg acaaggccgg gactttggag
     6361 gagacgctgc ggcaggcatt gaggcaggtt gacgtggtga tcacgaccgg cggagtgtcc
     6421 atgggtgagc tggatctcct caagccgacg atagagcgtt cgctgggcgg aacaattcgc
     6481 tttggtcggg tggcaatgaa gcccggcaag cctaccacgt ttgctaccgt tccggtaaag
     6541 gataacgacg gagagagagt ttccaaggta atcttctcgc taccaggtaa cccggcatcg
     6601 gctttggtta cgtttcactt gtttgtactg ccgtctctgc accaaatgtc gggcgtctca
     6661 ccacctggct tgccaaaggt gcctgtgaca ctgggccatg atttctccct cgatagatct
     6721 agacccgagt accaccgggc cattgtctcc gttagcaggg acggagtgct caccgcggtc
     6781 agcacaggag gacagagaag ctcgcgggta ggaagtatga agggggccaa cgcgcttctc
     6841 tgcatgcctt gcggaccaga gccgttgcgc aagggtgcca aggtggaagc gttgcttatg
     6901 ggcggtttac gcagcgatgt gatttaagaa ttcgatatca agcttatcga taccgtcgac
     6961 ctcgaggggg ggcccggtac cagcttttgt tccctttagt gagggttaat agggcgtgca
     7021 cggctatggg gtctgaatgc taaaagacac catttcccac actccctctg atttttttta
     7081 tggttttttt ttatcttcat ttttcgccgt cttttattcg gttgtttggg aggttcacat
     7141 gcaacgtgca agactgcaag tatattgaga tgggcgcttc atgcaatgca gcatcactgc
     7201 agcgacattg tgacatccac gtttcgatcg tctgtgcttc cccgttcatg tacgcatgat
     7261 ctgaaaaaaa aaaaaaaaaa aaaaaaagtt gatgtaataa tgaaaataaa cacccaggga
     7321 cgagcacccc ttctggaata gggagaaaaa ctaaagggac gtttgggtct ctacacttac
     7381 caacagattc atgtactgat cttcagcctg tacagaacag tacaatgtcc aaaactcgtt
     7441 atcattactc tatgtgccca aaatgaccca gatatacatc aacccactac atccttttgt
     7501 tcttgttttt tttttttttt ttgctacttc gaaaagaatt tccgtttagt tgctcttgct
     7561 catgtgctca agacggatgc tgaccgccct tctgtgagcc tcgagctcct caaccttagc
     7621 caactgcatg acagcctggc cgacgttttt gagaccctcg gcagtcaagt tggagctggt
     7681 aatgtgcttg acgaacgagg cgagattgac gccagagtac tgcttggcaa agccataggt
     7741 agctatgtgt cttgacgtta gcagaagaat ctgtgaagcg gggctattta tgatatgtaa
     7801 cttacgcagc gagtggttaa caccagcaga gtaatcgcca acggactcag gagtccaggc
     7861 gccaatgaag acactaccgg cgttcatgac aagatcgaca gccttctcgg cctccttgat
     7921 ctggaggatc aagtgctcag gagcgtactt gttgctgagc tccatggcct cctcgacggt
     7981 cttgacctgc acggtgatcg agtgggcgat ggagccacgg acaatctgga cgcgaggaag
     8041 ctccgtagcc tgacggtgaa cctcgtcctc aatagcctga agatgctcct cgtcgaggtc
     8101 aatagcgatc aggatgacct gactgtcaac gccgtgctca gcctgggaca ggagatccga
     8161 ggcaacgaac gcggggttgg cgtccttgtc agcgatgacc agcacctcgg acgggccagc
     8221 gggcatgtca ataccaacgg cagcgttggt gtcgttgctg acgaacatct tggcagcagt
     8281 gacgaactgg ttaccggggc cgagaatctt gtcgaccttg gtgatgctct cggtgccgta
     8341 ggccatggca gctacggcct gggcaccgcc ggcaagcacg atggactcgg cgccaacctt
     8401 gtgagcgacg tagacaatct cgggagtgat ggttccgtcg gcgcggggag gagaggcgaa
     8461 cacaatcttg ttgcagccgg cgaccatggc gggaacaccc agcataaggg cagtgctggg
     8521 gagaacggcg gtaccgccgg ggatgtagca gccgacggcc tcgatgggac gagagaaacg
     8581 gctgcagaca acaccgggca tggtctcgac ctggaggggc ttctcctcct tctgggcggc
     8641 gtggaacttg cggatgttct cgaaggacac gtcgatggca gcaatggtct cctcagggag
     8701 ctgcataagc tccttgggga agggcgcctt caggacgggg ctagtaagag aggtagcctt
     8761 ctcgaacttg tgagtgtacg acagaacagc cttgtcgccg ttcttgcgga cgtcctcgat
     8821 gatggggaca atgatcttgt agatggcatc ggacgacttt tgcgcaggac gcttgagagc
     8881 agcatcgagc tcctcggtag agaccttgga ggcgtcgaaa cgtctcatgg tgatcttctc
     8941 aggggtggtc tcctgggcgg cctccttggt gacaggggcc gaagtggcag cgagagcgga
     9001 cgccgcaggc ttgatgccct ccttctcagc ccacttaccc ttagcatctc cagtcctgcg
     9061 cttgaccttc cagctcttgg cgtcaaggct cctttcgata tcggcaagag taacgccggc
     9121 agcaacggcc ctggtaagag caaagtagaa gagatcggca gcctcaaagg cgatttcctg
     9181 gggggtctga gcggtgcaga gctcctcagc ctcctccatg atcttggccc ggactagctt
     9241 ctcatcggag aagagacggg cagtgtagga gccctcgggg gcagactgtt tcctcgaaat
     9301 caaagtctgc tcgagcttgg gaaggccttt gagctgacca aagcagccgg actgatcgag
     9361 gtggcagaaa cgacccttct gcttcacgac aaacttgaga gcatcgttat cgcagtcaag
     9421 cgagatgcgg acgagctcct gagtgtctcc ggaagtagca cccttgtacc agagaccgcg
     9481 cttccggctc tgatagacac cagtctgtgt cctgagggcc tcgttcacac tctcggcact
     9541 gctgtagacc agacccagag cagtgtcgtg ctcatcaacg acaacggtgg ggagaagacc
     9601 atcgggacgg tcagacttcc agacgctcga gaggatggtg gagatggcaa gcttcagctg
     9661 ctcgagttc
//
