LOCUS       sequence-459561-        9665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NIT-9 domains in reverse orientation with CNX1 linkage
            region in N. crassa at the his-3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9665)
  TITLE     Convergent Evolution Links Molybdenum Insertase Domains with 
            Organism-Specific Sequences.
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCCG_nit9fl_CNX1_linker_reverse
FEATURES             Location/Qualifiers
     source          1..9665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2343..2361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2636..2647
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4006..4024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4978..4994
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4978..4994
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6947..6963)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6948..6964)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(join(9664..9665,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(9665,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccgcgcg ttggccgatt cattaatgca ggttaacctg gcttatcgaa
     2341 attaatacga ctcactatag ggagaccggc agatccaatg cggatggatt cgctcactcg
     2401 gtgccgcggc ggtgcgaagt tcctgccgtg gattggggga ttgcacagac ggtggagtgc
     2461 ttgctacagc taggtaccca gttgcacacc aaacggtgcc cctgtgcccc tgcgtctagg
     2521 tgtgtcctgt gagcaactgc gcccgcgcgc ggcaccggga agctgcggga acctcaatgg
     2581 atgattggcg ccagatcccc ggcatcacct cagcacatgg gcagcccgag aaggaatcga
     2641 gggtagaaga tattccaggg tttccgctag cacaacttcc aagcgcctct accgctactc
     2701 cacacaccca cgctgaatgg cacaaccact gaggcctagc ggtcccagct ccccgtaacc
     2761 acgcagcccc agcgaccgag gtcatcatga cactatgacg acaatctggc gggagacttg
     2821 ggaaaatcga tgacgggcac ctaccttggg aacaatagag caagtccagt caacgcccta
     2881 gggaaggggg aaaaaaaaaa aaagagccca ttctcacaag gagcaacact gtgctgctat
     2941 catagtacac tacaagcaaa gtcgggatgt tgctagaatc cgtcggctac gtcggtagat
     3001 acctcccata ggcagtaagt ctaggtatcc aatttatagt tccgcgtatt tcttcgtcga
     3061 acggggcaca gcaaagccta acagccgcag tgcctcgcaa ccaacaccaa gcagtaatct
     3121 catccatcca tacagatcct cccacaatat ctttacatgc gcacttgcgc tgtgttagca
     3181 gtttaggttc cactaccaag gctgacacga gcagcactga cagcaaactg ctgcttgggg
     3241 ccaagaaatt ggcagtttgc tgagcaccta catatcagta cacatcaacc ctggtttaat
     3301 tgttagcctg gtactgttaa ccgtcatgaa cgaggacgtg gaggaagaac cacgctaaca
     3361 attgaaccag ggttgatgta tacctctctt accatatttg cttgcttcca cgtccctatc
     3421 ctcaagcgat tttgttcttg actttttgta tttcaacgca aaggccattc aggtttctca
     3481 tgttcaaccc tttggatggc gatgccgttc gtcccaggac ctcgtgacct ttgaaagacg
     3541 agaaatatat cccaaagcaa cttctgctta tggacaagca actgcatggc ggcccacaac
     3601 tcatatctcg cgtaatcatc caaacgctag gcccgagtac tctactgttc tcgagacacc
     3661 taccataata tgtacccaat cagacatgac aacagtcaac caaacatacc ctgatccggc
     3721 caaaccctga catccctaga tgatggttag gcacacaaat ggaaaacggg actcgttggt
     3781 taggtgggaa cgcttgtatt gcagccagcc aaccgactct cctcgtccct ctcgagtccc
     3841 gttattgccg tttgacccag ccgcccagcc gccaaacagc tgagggagcc aatggtctaa
     3901 tacaccgtcc aacggcccca gtgatgtttc aaacttgatt gacagcgaac gaaacccctg
     3961 aaacccgtta tagatatgta tagatttgac ggggtagctt ggccctaata cgactcacta
     4021 tagggcgaat tggagctcca ccgcggtggc ggccgctaga aggagcagtc catctgcgtg
     4081 aatcacgaga gaatcagcta ctttgaatcg atggatgcag ctaccagaag tcactcagtt
     4141 cgttcaaagc cacatcactg ggcacttcca ttgggacagg cattgatcgg acgagaccga
     4201 cttctggccg ctttcaacag ccacattata tccatgtcac ggctacgcgc ggccttcggt
     4261 aacagaaaag cacacagaca gcgattgtga catggattcg ggcaaagcat tggtggtcgc
     4321 accaggtcat cctgagtgtg cagtggctgc tattcagatt tcatctaact gcgggagagg
     4381 ggttcaaagg ggcgtgacgt cacagacaac gggtgaagga cgaagattgc ctcacttctt
     4441 tgctagcaat ttgcctgcaa agaaggcgca catgtcaagc aaacaactgg gaaaccacta
     4501 ttgaataccc acaatgcaaa gctcggaagg tacgtcttga ttgcagtgtg tcgagtgtca
     4561 aaaaagaagc aagtgttcat gcaagccaaa attggcacct cctccacttc tccgagtgcc
     4621 ccacccgaac ctccaggcga gatggccgga acataccatc cgcgttggga ttatgacgta
     4681 tctccttctt cttcacatga ttccatcccg ttgttgcttg tttgcgagct gtgacgggag
     4741 atcgtagatg ccacttaggg ccaggcaggc agtgcaggca gccaggaaca caagcttcca
     4801 acttggtcat ctcgattgcc gattaaggga accaaatgcc tatataagac tgtcctccca
     4861 cctccccaat accattcttt tcttcttcca tcatcagcca acaaagcaat cacatcttca
     4921 ctacttcaaa tcaacacaac actcaaacca ctttcacaac ccctcacatc aaccaaatct
     4981 agaactagtg gatccccggg ttaattaaca tgctctctgt ctccgaagcg ctctcccaaa
     5041 tagcgcaaca cacgccgatg ccgcaaatcg tcacctccaa agtcgatagc tcgctcatcg
     5101 gcgccgttct ggcgcaggac gtttcggcgc gcgaaaatgt gcccgctttt cgcgctagca
     5161 tcgtcgacgg ctacgccgta gtcgtgcccc aagacgggaa catgcgcggc gtgttccccg
     5221 tcgtgtcggt ttcgcatgcc gctcctggag atggggaaaa aatgccgacg ctcaaagagg
     5281 gcgagattgc ccggatcacc acgggtgcac ccctgcctcc gggtgcgaca tcggttatta
     5341 tggtggagga tacggtgttg gtgtcgacga cgtcatccaa gacggaggat gcggaggatg
     5401 gaaagggagt ggaggaggag aaggaggtgg agattttagc agctgataaa gttcgcgaag
     5461 gcgaaaatgt tagggaggtg ggaagtgata tcaagcaggg gcaactgatt ctaggcaagg
     5521 gagatcaggt gtctgctgtg gggggtgaga ttggtctgtt ggccgccgtg ggtgtgggtg
     5581 aggtgcaggt gtaccgacgt cctgttgttg gcgttctatc tacgggtgat gagattgtgg
     5641 accatgatcg cccgggagac ttgcggctgg gcgaggtgcg cgatacgaac cggccaactc
     5701 tcatttcggc ggcgagagat tgggggtatg aggtgattga tcttggagtg gcgcgggaca
     5761 aggccgggac tttggaggag acgctgcggc aggcattgag gcaggttgac gtggtgatca
     5821 cgaccggcgg agtgtccatg ggtgagctgg atctcctcaa gccgacgata gagcgttcgc
     5881 tgggcggaac aattcgcttt ggtcgggtgg caatgaagcc cggcaagcct accacgtttg
     5941 ctaccgttcc ggtaaaggat aacgacggag agagagtttc caaggtaatc ttctcgctac
     6001 caggtaaccc ggcatcggct ttggttacgt ttcacttgtt tgtactgccg tctctgcacc
     6061 aaatgtcggg cgtctcacca cctggcttgc caaaggtgcc tgtgacactg ggccatgatt
     6121 tctccctcga tagatctaga cccgagtacc accgggccat tgtctccgtt agcagggacg
     6181 gagtgctcac cgcggtcagc acaggaggac agagaagctc gcgggtagga agtatgaagg
     6241 gggccaacgc gcttctctgc atgccttgcg gaccagagcc gttgcgcaag ggtgccaagg
     6301 tggaagcgtt gcttatgggc ggtttacgca gcgatgtgat tattcggaaa gaaaagaagt
     6361 acgatgaagt acctatgtcc tcaagtacta ctccagcagc gctcaaagca gccatcctta
     6421 tcgtttcaac gacagcagct caagaccccg caacggactc ggccgacgtc actctccgcc
     6481 aggtcttcga tgaacaacaa gcccaatggg aggtccatga atccaaaatc gttcccgacg
     6541 atgtcgtgca aatccagaag caaatcatcc actggacaaa ccaagccacc gccgcaagtg
     6601 gcgacaacaa caacaacaac aacaccaaca ccatcaacct catcgtaacc acaggcggca
     6661 ccggcttcgc cgtgtccgac caaaccaccg aagccgtctc ggctctccta cacaagaatg
     6721 ctcctggcct ggtccacggc atgctggcgg cttcgctgca agtgaccccc tttgccatga
     6781 tgtcacgacc tgtggctggc gtcaggaacg gcaccgttat cgttgcgctg cctgggtccc
     6841 ccaagggcgc gaaggagaat ctgcaagcag tgatcaaaat gctgccccac gcttgcatgc
     6901 aggctgctgg tgctaactct agataagaat tcgatatcaa gcttatcgat accgtcgacc
     6961 tcgagggggg gcccggtacc agcttttgtt ccctttagtg agggttaata gggcgtgcac
     7021 ggctatgggg tctgaatgct aaaagacacc atttcccaca ctccctctga ttttttttat
     7081 ggtttttttt tatcttcatt tttcgccgtc ttttattcgg ttgtttggga ggttcacatg
     7141 caacgtgcaa gactgcaagt atattgagat gggcgcttca tgcaatgcag catcactgca
     7201 gcgacattgt gacatccacg tttcgatcgt ctgtgcttcc ccgttcatgt acgcatgatc
     7261 tgaaaaaaaa aaaaaaaaaa aaagttgatg taataatgaa aataaacacc cagggacgag
     7321 caccccttct ggaataggga gaaaaactaa agggacgttt gggtctctac acttaccaac
     7381 agattcatgt actgatcttc agcctgtaca gaacagtaca atgtccaaaa ctcgttatca
     7441 ttactctatg tgcccaaaat gacccagata tacatcaacc cactacatcc ttttgttctt
     7501 gttttttttt ttttttttgc tacttcgaaa agaatttccg tttagttgct cttgctcatg
     7561 tgctcaagac ggatgctgac cgcccttctg tgagcctcga gctcctcaac cttagccaac
     7621 tgcatgacag cctggccgac gtttttgaga ccctcggcag tcaagttgga gctggtaatg
     7681 tgcttgacga acgaggcgag attgacgcca gagtactgct tggcaaagcc ataggtagct
     7741 atgtgtcttg acgttagcag aagaatctgt gaagcggggc tatttatgat atgtaactta
     7801 cgcagcgagt ggttaacacc agcagagtaa tcgccaacgg actcaggagt ccaggcgcca
     7861 atgaagacac taccggcgtt catgacaaga tcgacagcct tctcggcctc cttgatctgg
     7921 aggatcaagt gctcaggagc gtacttgttg ctgagctcca tggcctcctc gacggtcttg
     7981 acctgcacgg tgatcgagtg ggcgatggag ccacggacaa tctggacgcg aggaagctcc
     8041 gtagcctgac ggtgaacctc gtcctcaata gcctgaagat gctcctcgtc gaggtcaata
     8101 gcgatcagga tgacctgact gtcaacgccg tgctcagcct gggacaggag atccgaggca
     8161 acgaacgcgg ggttggcgtc cttgtcagcg atgaccagca cctcggacgg gccagcgggc
     8221 atgtcaatac caacggcagc gttggtgtcg ttgctgacga acatcttggc agcagtgacg
     8281 aactggttac cggggccgag aatcttgtcg accttggtga tgctctcggt gccgtaggcc
     8341 atggcagcta cggcctgggc accgccggca agcacgatgg actcggcgcc aaccttgtga
     8401 gcgacgtaga caatctcggg agtgatggtt ccgtcggcgc ggggaggaga ggcgaacaca
     8461 atcttgttgc agccggcgac catggcggga acacccagca taagggcagt gctggggaga
     8521 acggcggtac cgccggggat gtagcagccg acggcctcga tgggacgaga gaaacggctg
     8581 cagacaacac cgggcatggt ctcgacctgg aggggcttct cctccttctg ggcggcgtgg
     8641 aacttgcgga tgttctcgaa ggacacgtcg atggcagcaa tggtctcctc agggagctgc
     8701 ataagctcct tggggaaggg cgccttcagg acggggctag taagagaggt agccttctcg
     8761 aacttgtgag tgtacgacag aacagccttg tcgccgttct tgcggacgtc ctcgatgatg
     8821 gggacaatga tcttgtagat ggcatcggac gacttttgcg caggacgctt gagagcagca
     8881 tcgagctcct cggtagagac cttggaggcg tcgaaacgtc tcatggtgat cttctcaggg
     8941 gtggtctcct gggcggcctc cttggtgaca ggggccgaag tggcagcgag agcggacgcc
     9001 gcaggcttga tgccctcctt ctcagcccac ttacccttag catctccagt cctgcgcttg
     9061 accttccagc tcttggcgtc aaggctcctt tcgatatcgg caagagtaac gccggcagca
     9121 acggccctgg taagagcaaa gtagaagaga tcggcagcct caaaggcgat ttcctggggg
     9181 gtctgagcgg tgcagagctc ctcagcctcc tccatgatct tggcccggac tagcttctca
     9241 tcggagaaga gacgggcagt gtaggagccc tcgggggcag actgtttcct cgaaatcaaa
     9301 gtctgctcga gcttgggaag gcctttgagc tgaccaaagc agccggactg atcgaggtgg
     9361 cagaaacgac ccttctgctt cacgacaaac ttgagagcat cgttatcgca gtcaagcgag
     9421 atgcggacga gctcctgagt gtctccggaa gtagcaccct tgtaccagag accgcgcttc
     9481 cggctctgat agacaccagt ctgtgtcctg agggcctcgt tcacactctc ggcactgctg
     9541 tagaccagac ccagagcagt gtcgtgctca tcaacgacaa cggtggggag aagaccatcg
     9601 ggacggtcag acttccagac gctcgagagg atggtggaga tggcaagctt cagctgctcg
     9661 agttc
//
