LOCUS       sequence-459575-       11747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PLAG1 with C-terminal 3xFLAG tag, in doxycycline-inducible
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11747)
  TITLE     Lentiviral expression for inducible PLAG1-3xFLAG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: iPLAG1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..11747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(3604..3622,3623..3645,3646..3664,3665..3687,3688..3706,
                     3707..3729,3730..3748,3749..3773,3774..3792,3793..3815,
                     3816..3834,3835..3857,3858..3876)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3909..3947
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3944..3968
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4077..4101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5624..5689
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5693..5717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5770..5796
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5882..7093
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6930..6949
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7152..7769,7770..7775,7776..7811,7812..7814,7815..7850,
                     7851..7853,7854..7889,7890..7898)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7951..8524
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8118..8135)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8345..8364
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8540..9334
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8540..9334
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8594..8613)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9204..9223
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9511..10099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9564..9584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9982..9993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10174..10407
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10526..10544)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10612..10716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10717..10785,10786..11577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10935..10954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag gatcctttac
     3601 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     3661 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     3721 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtgtttac cactccctat
     3781 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3841 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc
     3901 cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     3961 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     4021 gatccagcct ccgcggcccc gaactagtcc agtgtggtgg aattctgcag atatcaacaa
     4081 gtttgtacaa aaaagcaggc tagaaggcct ggtttaggct gcgatggcca ctgtcattcc
     4141 tggtgatttg tcagaagtaa gagataccca gaaagtccct tcagggaaac gtaagcgtgg
     4201 tgaaaccaaa ccaagaaaaa actttccttg ccaactgtgt gacaaggcct ttaacagtgt
     4261 tgagaaatta aaggttcact cctactctca cacaggagag aggccctaca agtgcataca
     4321 acaagactgc accaaggcct ttgtttctaa gtacaaatta caaaggcaca tggctactca
     4381 ttctcctgag aaaacccaca agtgtaatta ttgtgagaaa atgtttcacc ggaaagatca
     4441 tctgaagaat cacctccata cacacgaccc taacaaagag acgtttaagt gcgaagaatg
     4501 tggcaagaac tacaatacca agcttggatt taaacgtcac ttggccttgc atgccgcaac
     4561 aagtggtgac ctcacctgta aggtatgttt gcaaactttt gaaagcacgg gagtgcttct
     4621 ggagcacctt aaatctcatg caggcaagtc gtctggtggg gttaaagaaa aaaagcacca
     4681 gtgcgaacat tgtgatcgcc ggttctacac ccgaaaggat gtccggagac acatggtggt
     4741 gcacactgga agaaaggact tcctctgtca gtattgtgca cagagatttg ggcgaaagga
     4801 tcacctgact cgacatatga agaagagtca caatcaagag cttctgaagg tcaaaacaga
     4861 accagtggat ttccttgacc catttacctg caatgtgtct gtgcctataa aagacgagct
     4921 ccttccggtg atgtccttac cttccagtga actgttatca aagccattca caaacacttt
     4981 gcagttaaac ctctacaaca ctccatttca gtccatgcag agctcgggat ctgcccacca
     5041 aatgatcaca actttacctt tgggaatgac atgcccaata gatatggaca ctgttcatcc
     5101 ctctcaccac ctttctttca aatatccgtt cagttctacc tcatatgcaa tttctattcc
     5161 tgaaaaagaa cagccattaa agggggaaat tgagagttac ctgatggagt tacaaggtgg
     5221 cgtgccctct tcatcccaag attctcaagc atcgtcatca tctaagctag ggttggatcc
     5281 tcagattggg tccctagatg atggtgcagg agacctctcc ctatccaaaa gctctatctc
     5341 catcagtgac cccctaaaca caccagcatt ggatttttct cagttgttta atttcatacc
     5401 tttaaatggt cctccctata atcctctatc agtggggagc cttggaatga gctattccca
     5461 ggaagaagca cattcttctg tttcccagct ccccccacaa acacaggatc ttcaagatcc
     5521 tgcaaacact atagggcttg ggtctctgca ctcactgtca gcagctttca ccagcagttt
     5581 aagcacaagt accaccctcc cacgtttcca tcaagctttt caggactaca aggaccacga
     5641 cggtgactac aaggaccacg acatcgacta caaggacgac gacgacaagt agacccagct
     5701 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     5761 cggttcgaat acccatacga cgtcccagac tacgcttagt aatgattaat taaactagaa
     5821 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagtaaccc acccaagatc
     5881 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     5941 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
     6001 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     6061 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     6121 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     6181 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     6241 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
     6301 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
     6361 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     6421 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
     6481 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     6541 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     6601 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     6661 ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt
     6721 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     6781 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     6841 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     6901 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     6961 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     7021 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     7081 ttttttgtta gacgaagctt ggtaccgagc tcggatctcc accccgtacc ggtcctgcag
     7141 tcgaattcac catgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac
     7201 tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     7261 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     7321 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     7381 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
     7441 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     7501 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     7561 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     7621 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     7681 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     7741 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
     7801 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     7861 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat ccgcggccgc
     7921 actagaggaa ttccgcccct ctcccccccc cccctctccc tccccccccc ctaacgttac
     7981 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     8041 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     8101 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     8161 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     8221 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     8281 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     8341 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     8401 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     8461 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     8521 ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     8581 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     8641 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     8701 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     8761 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     8821 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     8881 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     8941 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     9001 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     9061 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     9121 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     9181 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     9241 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     9301 cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct
     9361 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9421 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa
     9481 ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa
     9541 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9601 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9661 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9721 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9781 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9841 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9901 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9961 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10021 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10081 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
    10141 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    10201 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10261 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10321 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10381 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
    10441 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
    10501 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    10561 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    10621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    10741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10921 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    10981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    11041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    11101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    11161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    11221 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    11281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    11341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    11401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    11461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    11521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    11581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    11701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
