LOCUS       sequence-459684-       14306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C>T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Kaplan EG, Steger RJ, Shah ST, Drepanos LM, Griffith AL, Reint G, 
            Doench JG
  TITLE     Activity-based selection for enhanced base editor mutational 
            scanning.
  JOURNAL   Nat Genet. 2025 Nov;57(11):2920-2929. doi: 
            10.1038/s41588-025-02366-0. Epub 2025 Oct 14.
  PUBMED    41087678
REFERENCE   2  (bases 1 to 14306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-025-02366-0"; journalName: "Nat Genet"; date: 
            "2025-11"; volume: "57"; issue: "11"; pages: "2920-2929"
COMMENT     Sequence Label: pRDB_092
FEATURES             Location/Qualifiers
     source          1..14306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2640..3818
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2871..3809
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3766..3786
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3865..3885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4483..8583
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8614..8862
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8875..8922
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8929..8982
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8989..9387
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9398..9986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9451..9471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9869..9880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10058..10291
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10317..10334)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10323..10547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10593..11021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10680..10699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10890..10911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11030..11050)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11035..11364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11215..11350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11277..11296
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11412..11459
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11478..11852
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11982..12103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12019..12038)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12073..12092
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12152..12168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12152..12168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12165..12187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12176..12192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12200..12206,12207..12224,12225..12230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12245..12266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12383..12400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12554..13142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12634..12653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13313..14104,14105..14173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13936..13955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14174..14278)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccggg cagaggtctc gcttttttcg agacaacacg
     2641 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2701 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2761 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2821 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     2881 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     2941 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3001 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     3061 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3121 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3181 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3241 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3301 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3361 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3421 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3481 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3541 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3601 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3661 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3721 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     3781 tggttcaaag tttttttctt ccatttcagg tgtcgtgaag gagccaccat gaaacggaca
     3841 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagttc tgaggtggag
     3901 ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc acgggatgag
     3961 aggaaggcgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg
     4021 aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatagc cctgagacag
     4081 ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgt gacattcgag
     4141 ccttgcgtga tgtgcgccgg cgccatgatc aactctagga tcggccgcgt ggtgtttggc
     4201 gtgaggaact caaaaagagg cgccgcaggc tccctgatga acgtgctgaa ctaccccgga
     4261 atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg
     4321 tgcgatttct atcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc
     4381 atcaactctg gcggatctag cggaggatcc tctggcagcg agacaccagg aacaagcgag
     4441 tcagcaacac cagagagcag tggcggcagc agcggcggca gcgacaagaa gtacagcatc
     4501 ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     4561 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     4621 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     4681 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     4741 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     4801 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     4861 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     4921 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     4981 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     5041 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgctag cggcgtggac
     5101 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     5161 cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct gagcctgggc
     5221 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     5281 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     5341 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     5401 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     5461 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     5521 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     5581 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     5641 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     5701 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     5761 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     5821 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     5881 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     5941 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     6001 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     6061 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     6121 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     6181 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     6241 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     6301 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     6361 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     6421 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     6481 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     6541 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     6601 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     6661 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     6721 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     6781 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     6841 atcaaagagc tgggcagcca gatcctgaaa gaacacccag tggaaaacac ccagctgcag
     6901 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     6961 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
     7021 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     7081 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     7141 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     7201 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     7261 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     7321 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     7381 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     7441 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     7501 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     7561 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     7621 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     7681 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     7741 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     7801 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     7861 cctaagaagt acggcggctt cctgtggccc accgtggcct attctgtgct ggtggtggcc
     7921 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
     7981 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     8041 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     8101 aacggccgga agagaatgct ggcctctgcc aagcagctgc agaagggaaa cgaactggcc
     8161 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     8221 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     8281 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     8341 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     8401 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     8461 gacaccacca tcgaccggaa gcagtacaga agcaccaaag aggtgctgga cgccaccctg
     8521 atccaccaga gcatcaccgg cctgtacgag acacgaatcg atttgtctca gctgggaggc
     8581 gatgggtccg ggagcggcgg gagcgggggg agcactaatc tgagcgacat cattgagaag
     8641 gagactggga aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag
     8701 gaagtgatcg gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc
     8761 acagatgaga atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg
     8821 gtcatccagg attctaacgg cgagaataag atcaagatgc tgagcggagg ctccaaaagg
     8881 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggaagcga gggcagagga
     8941 agccttctaa catgcggtga cgtggaggag aatcccggcc cttccgggat ggccaagcct
     9001 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     9061 atctctgagg actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     9121 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     9181 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     9241 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     9301 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     9361 ccctctggtt atgtgtggga gggctaataa attcgaaaat caacctctgg attacaaaat
     9421 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     9481 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     9541 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     9601 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     9661 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     9721 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     9781 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     9841 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     9901 cggcctgctg ccggctctgc ggcctcttcc gcgtcctcgc cttcgccctc agacgagtcg
     9961 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
    10021 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
    10081 cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
    10141 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    10201 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10261 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
    10321 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    10381 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    10441 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    10501 ttgggaagat aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    10561 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
    10621 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    10681 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    10741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    10801 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    10861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10921 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
    11041 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    11101 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    11161 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
    11221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    11281 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
    11341 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
    11401 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
    11461 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
    11521 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
    11581 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
    11641 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
    11701 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
    11761 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
    11821 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
    11881 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    11941 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    12001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    12061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    12121 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    12181 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    12241 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    12301 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    12361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    12421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    12481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    12541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    12601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    12661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    12721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    12781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    12841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    12901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    12961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    13021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    13081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    13141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    13201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    13261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    13321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    13381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    13441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    13501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    13561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    13621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    13681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    13741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    13801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    13861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    13921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    13981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    14041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    14101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    14161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    14221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    14281 catttccccg aaaagtgcca cctgac
//
