LOCUS       sequence-459688-        5917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2135m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific brain cell types.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14703 - pAAV-AiE2135m-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..5917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1875..1884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1887..1907
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2973..3084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3395..3850
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3482..3501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3692..3713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3867..3886)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3986..4008
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4046..4064)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4132..4236
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4237..4305,4306..5097)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4455..4474)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5268..5856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5757..5776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gccggcgtcg ggaacatggg
      181 ggtcaagtga gagagccagg tgtctccagc actcagcatg atatctgaca caaatcagac
      241 ccctcacttt taacactgta ccctaagact tggggcacag gccagggact ttccgttcta
      301 ggctctctgg ctcctgcttc ttggattctg atctccggtc ttatcgactt catttccaaa
      361 ttagaggttt tttggaaccc aggggctacc aggccaacct ggaagtccga ctgcacatgc
      421 tgtatccgtg acagagttac agagaacagg gactgccctg agtgggacag gccgaagagg
      481 gactgccaga gaggtacaga ccccaccccc acctttgtaa gcctgattgc ctacccaggc
      541 caccacaact ccatccagct ctgtccccgc ctttgttcct gagtctccaa ggcccccttt
      601 cctgccaaac aaagccccca gccacccgac tgccgcccaa caggcacctg gggcctctgg
      661 gcctgggaat caaagcctgt tttgcaaagg aggcccagtg tttggagaac acccatcccc
      721 tgctgcccgg tccttcaaag aaccggcatt gttctgcgct gagaaggcgc ctcttgacgc
      781 gctgggcagg gtcggcttga cgctggtgga cctctgatct caggccaggc ccccagccac
      841 ttggggggag ggtggactta ggggccaaag agggagcaac ctgtccttgg ggcactgtgg
      901 tccttcctca ttctctgccc acccgagttc ccatcaccga gttcattgag acccaggctc
      961 ttctacctgg tgatctggac aaggcaggcc tcaggaggca ccaggacaag agatgagata
     1021 gcgggcagga ttttagtgac cataacaggt acgtcagaga ctgcaaacgt attaggcctc
     1081 agtggcatgt aactgtgatt ctagtacttg ggatgctgag gcaggagaat catgagtttg
     1141 aagttaaccg gatcttcaag gcaagaccct gtctctaaat catagatcac aggggagtgt
     1201 gtgtttgtgt gtgtgtgtgt gcgtgcgtgc gcgcatgcgc gtgtacacgt gcgtgtgcat
     1261 atgtatgcat gttctcattt tacagctcac agaggctaga gaggaaactg ggcccagagc
     1321 ccggttcaag ctgtgaactg acaggccccg ttggctcttt tatttgttga agaaatattt
     1381 cccaattact ttcttgtcca ttgtggattc cactgcaagc taacattagt aaaggcattt
     1441 aaataaaagt ctgcccagac acaaactaag gccacagaac tggctgtggt gcccaggcgg
     1501 gtcgtgagga gcccaaataa cactcaaaag ccacctttat ttgtccttcc cctctgccag
     1561 tcacagcatc atgagcttct ggggtggtgt gtgtgtgggg ggggtgttag tctcactcct
     1621 gtggcactgc agaagtctct caaggcacag agagagggca agcaatgtgt tcaaagtcac
     1681 ccagaaactg aggttagatt ggagcggaga gcctcatctc aggagctcat tctgtccggt
     1741 cctcccctca gatcacactt ctgatcaagt ggcacggcag accttcgccg gcggagctcg
     1801 ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccag
     1861 atctttcgaa gctagccacc atggtgccca agaagaagag gaaagtctcc aacctgctga
     1921 ctgtgcacca aaacctgcct gccctccctg tggatgccac ctctgatgaa gtcaggaaga
     1981 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     2041 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa caacaggaaa tggttccctg
     2101 ctgaacctga ggatgtgagg gactacctcc tgtacctgca agccagaggc ctggctgtga
     2161 agaccatcca acagcacctg ggccagctca acatgctgca caggagatct ggcctgcctc
     2221 gcccttctga ctccaatgct gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg
     2281 atgctgggga gagagccaag caggccctgg cctttgaacg cactgacttt gaccaagtca
     2341 gatccctgat ggagaactct gacagatgcc aggacatcag gaacctggcc ttcctgggca
     2401 ttgcctacaa caccctgctg cgcattgccg aaattgccag aatcagagtg aaggacatct
     2461 cccgcaccga tggtgggaga atgctgatcc acattggcag gaccaagacc ctggtgtcca
     2521 cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa gctggtggag agatggatct
     2581 ctgtgtctgg tgtggctgat gaccccaaca actacctgtt ctgccgggtc agaaagaatg
     2641 gtgtggctgc cccttctgcc acctcccaac tgtccacccg ggccctggaa gggatctttg
     2701 aggccaccca ccgcctgatc tatggtgcca aggatgactc tgggcagaga tacctggcct
     2761 ggtctggcca ctctgccaga gtgggtgctg ccaccgacat ggccagggct ggtgtgtcca
     2821 tccctgaaat catgcaggct ggtggctgga ccaatgtgaa cattgtgatg aactacatca
     2881 gaaacctgga ctctgagact ggggccatgg tgaggctgct cgaagatggg gactgaggcg
     2941 cgccgaattc aagcttctcg agagatcttc gactgtgcct tctagttgcc agccatctgt
     3001 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3061 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3121 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgagatctc
     3181 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     3241 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccggcctcag tgagcgagcg
     3301 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3361 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     3421 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     3481 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3541 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3601 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3661 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3721 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     3781 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3841 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3901 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3961 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4021 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4081 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4141 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4201 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4261 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4321 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4381 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4441 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4501 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4561 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4621 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4681 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4741 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4801 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4861 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4921 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4981 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5041 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5101 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5161 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5221 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5281 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5341 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5401 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5461 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5521 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5581 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5641 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5701 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5761 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5821 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5881 cggttcctgg ccttttgctg gccttttgct cacatgt
//
