LOCUS       sequence-459735-        4850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty control vector for pAAV-Ucp1-Cre-miR122.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Behrens J, Braren I, Jaeckstein MY, Lilie L, Heine M, Sass F, Sommer
            J, Silbert-Wagner D, Fuh MM, Worthmann A, Straub L, Moustafa T, 
            Heeren J, Scheja L
  TITLE     An efficient AAV vector system of Rec2 serotype for intravenous 
            injection to study metabolism in brown adipocytes in vivo.
  JOURNAL   Mol Metab. 2024 Oct;88:101999. doi: 10.1016/j.molmet.2024.101999. 
            Epub 2024 Jul 31.
  PUBMED    39094948
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Metab."; date: "2024-10"; volume: "88:101999. doi"; pages: " 
            10.1016/j.molmet.2024.101999. Epub 2024 Jul 31"
COMMENT     Sequence Label: pAAV-Ucp1-empty-miR122
FEATURES             Location/Qualifiers
     source          1..4850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    55..643
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(108..128)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(526..537)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    675..1151
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(874..893)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   1191..1331
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1191..1320
                     /label=AAV2 ITR
     rep_origin      1406..1861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1493..1512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1703..1724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1878..1897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1997..2019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2057..2075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2143..2247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2248..2316,2317..3108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2466..2485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3279..3867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3768..3787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3929..4058
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 actccagatc aacaaacacc attgtcacac tccacgccgg cgaagcttat cgataatcaa
       61 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
      121 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
      181 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
      241 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
      301 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
      361 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
      421 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat
      481 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
      541 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
      601 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg
      661 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
      721 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
      781 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
      841 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
      901 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
      961 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     1021 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     1081 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     1141 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     1201 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     1261 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     1321 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     1381 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     1501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     1561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     1621 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1681 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     1741 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     1801 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     1861 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     1921 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     1981 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2041 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2101 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2161 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2221 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2281 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2341 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2401 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2461 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2521 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2581 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2641 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2701 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2761 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2821 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2881 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2941 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3001 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3061 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3121 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3181 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3421 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3901 gccttttgct ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact
     3961 gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     4021 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcacgc gtgacgtcac
     4081 agtgggtcag tcacccttga tcacactgca ccagtcttca cctttccacg cttcctgcca
     4141 gagcatgaat caggctctct ggggataccg gcctcacccc tactgaggca aactttctcc
     4201 cacttctcag aggctctgag ggcagcaagg tcagcccttt ctttggaatc tagaaccact
     4261 ccctgtcttg agctgacatc acagggcagg cagatgcagc agggaagggc ctgggactgg
     4321 gacgttcatc ctacaagaaa gctgtggaac ttttcagcaa catctcagaa atcagatcgc
     4381 acttattcaa aggagccagg ccctgctctg cgccctggtg gaggctcctc atgtgaagag
     4441 tgacaaaagg caccatgttg tggatacggg gcgaagcccc tccggtgtgt cctccaggca
     4501 tcatcaggaa ctagtgccaa agcagaggtg ctggccaggg ctttgggagt gacgcgcgtc
     4561 tgggaggctt gtgcgcccag ggcacgcccc tgccgattcc cactagcagg tcttggggga
     4621 cctgggccgg ctctgcccct cctccagcaa tcgggctata aagctcttcc aagtcagggc
     4681 gcagaagtgc cgggcgatcc gggcttaaag agcgagagga agggacgctc acctttgagc
     4741 tcctccacaa atagccctgg tggctgccac agaagttcga agttgagagt tcgggaattc
     4801 accggtcaaa caccattgtc acactccata ctatcaaaca ccattgtcac
//
