LOCUS       sequence-459741-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Circularly permuted CasRx platform fused to human deaminase domain 
            for A to I editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Wang Y, Liu KI, Liu MM, Ooi KH, Nguyen TA, Chee JE, Teo SXD, He S, 
            Tay JWD, Teo SY, Liew KS, Ge XY, Ng ZJ, Avagyan H, Liu H, Yi Z, 
            Chang K, Kok EPL, Chen R, Yau CE, Koh JW, Wan Y, Tan MH
  TITLE     A circularly permuted CasRx platform for efficient, site-specific 
            RNA editing.
  JOURNAL   Nat Biotechnol. 2024 Oct 9. doi: 10.1038/s41587-024-02430-w.
  PUBMED    39385008
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Oct 9. doi: 10.1038/s41587-024-02430-w."
COMMENT     Sequence Label: xPERT-hA2q
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..33
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(93..109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(106..128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    117..133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(141..147,148..165,166..171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(324..341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(495..1083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(575..594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1254..2045,2046..2114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1877..1896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2115..2219)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2294..2313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2485..2864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2865..3068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3019..3039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3113..3132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3113..3131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5072..5092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5192..5212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7483..7500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7489..7713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7759..8187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7846..7865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8056..8077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8196..8216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8201..8530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8381..8516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8443..8462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8597..9391
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8651..8670)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9261..9280
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    join(9565..9642,1..44)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9602..9621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
       61 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2221 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     2281 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     2341 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     2401 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     2461 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     2521 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2581 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2641 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2701 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2761 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2821 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2881 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2941 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3001 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     3061 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     3121 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttgcca ccatgaggaa
     3181 ggatatgtcc gagatcagga aaaatcataa ggtgttcgac tccatcagga ccaaggtcta
     3241 caccatgatg gactttgtga tttataggta ttacatcgaa gaggatgcca aggtggctgc
     3301 cgccaataag tccctccccg ataatgagaa gtccctgagc gagaaggata tctttgtgat
     3361 taacctgagg ggctccttca acgacgacca gaaggatgcc ctctactacg atgaagctaa
     3421 tagaatttgg agaaagctcg aaaatatcat gcacaacatc aaggaattta ggggaaacaa
     3481 gacaagagag tataagaaga aggacgcccc tagactgccc agaatcctgc ccgctggccg
     3541 tgatgtttcc gccttcagca aactcatgta tgccctgacc atgttcctgg atggcaagga
     3601 gatcaacgac ctcctgacca ccctgattaa taaattcgat aacatccaga gcttcctgaa
     3661 ggtgatgcct ctcatcggag tcaacgctaa gttcgtggag gaatacgcct ttttcaaaga
     3721 ctccgccaag atcgccgatg agctgaggct gatcaagtcc ttcgctagaa tgggagaacc
     3781 tattgccgat gccaggaggg ccatgtatat cgacgccatc cgtattttag gaaccaacct
     3841 gtcctatgat gagctcaagg ccctcgccga caccttttcc ctggacgaga acggaaacaa
     3901 gctcaagaaa ggcaagcacg gcatgagaaa tttcattatt aataacgtga tcagcaataa
     3961 aaggttccac tacctgatca gatacggtga tcctgcccac ctccatgaga tcgccaaaaa
     4021 cgaggccgtg gtgaagttcg tgctcggcag gatcgctgac atccagaaaa aacagggcca
     4081 gaacggcaag aaccagatcg acaggtacta cgaaacttgt atcggaaagg ataagggcaa
     4141 gagcgtgagc gaaaaggtgg acgctctcac aaagatcatc accggaatga actacgacca
     4201 attcgacaag aaaaggagcg tcattgagga caccggcagg gaaaacgccg agagggagaa
     4261 gtttaaaaag atcatcagcc tgtacctcac cgtgatctac cacatcctca agaatattgt
     4321 caatatcaac gccaggtacg tcatcggatt ccattgcgtc gagcgtgatg ctcaactgta
     4381 caaggagaaa ggctacgaca tcaatctcaa gaaactggaa gagaagggat tcagctccgt
     4441 caccaagctc tgcgctggca ttgatgaaac tgcccccgat aagagaaagg acgtggaaaa
     4501 ggagatggct gaaagagcca aggagagcat tgacagcctc gagagcgcca accccaagct
     4561 gtatgccaat tacatcaaat acagcgacga gaagaaagcc gaggagttca ccaggcagat
     4621 taacagggag aaggccaaaa ccgccctgaa cgcctacctg aggaacacca agtggaatgt
     4681 gatcatcagg gaggacctcc tgagaattga caacaagaca tgtaccctgt tcgcaaacaa
     4741 ggccgtcgcc ctggaagtgg ccaggtatgt ccacgcctat atcaacgaca ttgccgaggt
     4801 caattcctac ttccaactgt accattacat catgcagaga attatcatga atgagaggta
     4861 cgagaaaagc agcggaaagg tgtccgagta cttcgacgct gtgaatgacg agaagaagta
     4921 caacgatagg ctcctgaaac tgctgtgtgt gcctttcggc tactgtatcc ccaggtttct
     4981 gaacctgagc atcgaggccc tgttcgatag gaacgaggcc gccaagttcg acaaggagaa
     5041 aaagaaggtg tccggcaatt ccggatccgg acctaagaaa aagaggaagg tgtctggtgg
     5101 ttcttctggt ggttctagcg gatcagagac tcctggaacg tcagagagtg caacaccgga
     5161 atcatctggt ggttcttctg gtggttctag ccccaagaag aagagaaagg tggaggccag
     5221 catcgaaaaa aaaaagtcct tcgccaaggg catgggcgtg aagtccacac tcgtgtccgg
     5281 ctccaaagtg tacatgacaa ccttcgccga aggcagcgac gccaggctgg aaaagatcgt
     5341 ggagggcgac agcatcagga gcgtgaatga gggcgaggcc ttcagcgctg aaatggccga
     5401 taaaaacgcc ggctataaga tcggcaacgc caaattcagc catcctaagg gctacgccgt
     5461 ggtggctaac aaccctctgt atacaggacc cgtccagcag gatatgctcg gcctgaagga
     5521 aactctggaa aagaggtact tcggcgagag cgctgatggc aatgacaata tttgtatcca
     5581 ggtgatccat aacatcctgg acattgaaaa aatcctcgcc gaatacatta ccaacgccgc
     5641 ctacgccgtc aacaatatct ccggcctgga taaggacatt attggattcg gcaagttctc
     5701 cacagtgtat acctacgacg aattcaaaga ccccgagcac catagggccg ctttcaacaa
     5761 taacgataag ctcatcaacg ccatcaaggc ccagtatgac gagttcgaca acttcctcga
     5821 taaccccaga ctcggctatt tcggccaggc ctttttcagc aaggagggca gaaattacat
     5881 catcaattac ggcaacgaat gctatgacat tctggccctc ctgagcggac tggcgcactg
     5941 ggtggtcgct aacaacgaag aagagtccag gatctccagg acctggctct acaacctcga
     6001 taagaacctc gacaacgaat acatctccac cctcaactac ctctacgaca ggatcaccaa
     6061 tgagctgacc aactccttct ccaagaactc cgccgccaac gtgaactata ttgccgaaac
     6121 tctgggaatc aaccctgccg aattcgccga acaatatttc agattcagca ttatgaaaga
     6181 gcagaaaaac ctcggattca atatcaccaa gctcagggaa gtgatgctgg acagcggatc
     6241 agagactcct ggaacgtcag agagtgcaac accggaatca cagctgcatt taccgcaggt
     6301 tttagctgac gctgtctcac gcctggtcct gggtaagttt ggtgacctga ccgacaactt
     6361 ctcctcccct cacgctcgca gaaaagtgct ggctggagtc gtcatgacaa caggcacaga
     6421 tgttaaagat gccaaggtga taagtgtttc tacaggaaca aaatgtatta atggtgaata
     6481 catgagtgat cgtggccttg cattaaatga ctgccatgca gaaataatat ctcggagatc
     6541 cttgctcaga tttctttata cacaacttga gctttactta aataacaaag atgatcaaaa
     6601 aagatccatc tttcagaaat cagagcgagg ggggtttagg ctgaaggaga atgtccagtt
     6661 tcatctgtac atcagcacct ctccctgtgg agatgccaga atcttctcac cagacgagcc
     6721 aatcctggaa gaaccagcag atagacaccc aaatcgtaaa gcaagaggac agctacggac
     6781 caaaatagag tctggtcagg ggacgattcc agtgcgctcc aatgcgagca tccaaacgtg
     6841 ggacggggtg ctgcaagggg agcggctgct caccatgtcc tgcagtgaca agattgcacg
     6901 ctggaacgtg gtgggcatcc agggatcact gctcagcatt ttcgtggagc ccatttactt
     6961 ctcgagcatc atcctgggca gcctttacca cggggaccac ctttccaggg ccatgtacca
     7021 gcggatctcc aacatagagg acctgccacc tctctacacc ctcaacaagc ctttgctcag
     7081 tggcatcagc aatgcagaag cacggcagcc agggaaggcc cccaacttca gtgtcaactg
     7141 gacggtaggc gactccgcta ttgaggtcat caacgccacg actgggaagg atgagctggg
     7201 ccgcgcgtcc cgcctgtgta agcacgcgtt gtactgtcgc tggatgcgtg tgcacggcaa
     7261 ggttccctcc cacttactac gctccaagat taccaagccc aacgtgtacc atgagtccaa
     7321 gctggcggca aaggagtacc aggccgccaa ggcgcgtctg ttcacagcct tcatcaaggc
     7381 ggggctgggg gcctgggtgg agaagcccac cgagcaggac cagttctcac tcacgtaagc
     7441 ggccgctcga gcctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     7501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7681 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7741 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7801 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7861 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7921 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7981 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8041 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8101 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8161 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     8221 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8281 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8341 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8461 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8521 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     8581 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     8641 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     8701 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     8761 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     8821 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     8881 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     8941 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     9001 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     9061 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     9121 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     9181 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     9241 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     9301 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     9361 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     9421 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9481 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9541 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9601 agcaatagca tcacaaattt cacaaataaa gcattttttt ca
//
