LOCUS       sequence-459792-        2677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal integrating shuttle vector depleted of restriction sites 
            outside the polylinker region, yeast selection marker HIS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Scutteri L, Barth P, Rahi SJ
  TITLE     Minimal integrating shuttle vectors for Saccharomyces cerevisiae 
            depleted of restriction sites outside the polylinker region
  JOURNAL   bioRxiv 2024.11.05.622133
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.05.622133"
COMMENT     Sequence Label: pLS403
FEATURES             Location/Qualifiers
     source          1..2677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(283..302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1437..1456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1537..1553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1537..1553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1610..1626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1627..1814
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             1815..2477
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaacagg aaacagctat gacgcgcgcg
     1561 agctcggtac ccgggatcct ctagagtcga cctgcaggca tgccctagga ctggccgtcg
     1621 ttttacctag tacactctat atttttttat gcctcggtaa tgattttcat tttttttttt
     1681 ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat aatgaattat
     1741 acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag caggcaagat
     1801 aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca aatgaaacca
     1861 agattcagat tgcgatttct ttaaagggtg gtcccctagc gatagaacac tcgatcttcc
     1921 cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg attaacgtcc
     1981 acacaggtat agggtttctg gaccacatga ttcatgctct cgccaagcac tccggctggt
     2041 cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc actgaggact
     2101 gcgggattgc tctgggtcag gcttttaaag aggctctagg ggccgtgcgt ggagtaaaaa
     2161 ggtttggatc aggatttgcg cctttggatg aggcactttc cagggcggtt gttgatcttt
     2221 cgaacaggcc atacgcagtt gtcgaacttg gtttgcaaag ggagaaagta ggtgatctct
     2281 cttgcgagat gatcccgcat tttcttgaaa gttttgcaga ggcaagcaga attaccctcc
     2341 acgttgattg tctgcgaggc aagaatgacc atcaccgtag cgagagtgcg ttcaaggctc
     2401 ttgcggttgc cataagagaa gccacctcgc ccaatggaac caacgatgtt ccctccacca
     2461 aaggtgttct tatgtagtga caccgattat ttaaagctgc atcatacgat atatatatat
     2521 gtgtatatat gtatatctat gaatgtcagt aagtatgtat atgaacagta tgatactgaa
     2581 aatgacaagg taatacatca ttctatatgt gtcattctga acgaggcgcg ttttcctttt
     2641 ttctttttgc tttttctttt tttttctctt gaactcg
//
