LOCUS       sequence-459797-        4530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6
            polymerase .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Wilson MH, Hensley MR, Shen MC, Lu HY, Quinlivan VH, Busch-Nentwich 
            EM, Rawls JF, Farber SA
  TITLE     Zebrafish are resilient to the loss of major diacylglycerol 
            acyltransferase enzymes.
  JOURNAL   J Biol Chem. 2024 Nov 6;300(12):107973. doi: 
            10.1016/j.jbc.2024.107973.
  PUBMED    39510175
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2024-11-6"; volume: "300(12):107973. doi"; pages: " 
            10.1016/j.jbc.2024.107973"
COMMENT     Sequence Label: pcRII-mogat3a in situ probe
FEATURES             Location/Qualifiers
     source          1..4530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     239..261
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     253..270
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     254..270
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     277..296
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        277..295
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1002..1020)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1003..1020)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1038..1054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1038..1054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1051..1073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1062..1078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1086..1092,1093..1110,1111..1116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1131..1152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1269..1286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1440..2028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1520..1539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2199..2990,2991..3059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2822..2841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3077..3871)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3188..3207)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3798..3817
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(join(4215..4530,1..113))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4325..4346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
       61 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
      121 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      181 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      241 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
      301 ttgggccctc tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattcg
      361 cccttgctgg ttggctgtat ctggaccggg acacgccctc atgtggaggt cggaggtcac
      421 agtgggtcag aagctggagg atctggacgc atttcaggga ttatttccca atcacactgg
      481 tcaagacggt ggatctggac ccgaggcaca attatctgct gggtttccat ccgcacggtg
      541 ttctggtggc cggtggattc ggaaacttct gcacggaggc gtccggattc tcccagatgt
      601 tcccgggact cacgccgtac ctgctgatgc tgccgttctg gttcagggtg cccttcttca
      661 gagaatacat catgtgcgga gggctggtgt ccagtgagaa ggccagtgcg agttacctgc
      721 tgggtcatcc aggcgggggt caagcggccg tcattgcagt gggcggagct ccggagtctc
      781 tggaggcgcg gcctggagca ctgacactgc aactgctgca gcgcaaaggc ttcatcaaac
      841 tcgccctcaa acacggagcg tggctggttc cagtgttctc gtttggggag aacgagctct
      901 tcgaccagat ggagaatccc gcaagggcga attccagcac actggcggcc gttactagtg
      961 gatccgagct cggtaccaag cttgatgcat agcttgagta ttctatagtg tcacctaaat
     1021 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1081 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1141 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1201 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1261 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1321 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1381 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1441 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1501 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1561 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1621 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1681 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1801 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1861 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1921 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1981 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2041 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2101 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2161 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2221 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2281 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2341 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2401 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2461 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2521 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2581 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2641 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2701 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2761 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2821 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2881 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2941 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3001 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3061 ctcttccttt ttcaattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3121 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     3181 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     3241 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     3301 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     3361 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     3421 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     3481 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     3541 cggcaggagc aaggtgggat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     3601 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     3661 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     3721 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     3781 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     3841 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     3901 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     3961 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     4021 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     4081 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     4141 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     4201 ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4261 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4321 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4381 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4441 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4501 ttgacgggga aagccggcga acgtggcgag
//
