LOCUS       sequence-459800-        2679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal integrating shuttle vector depleted of restriction sites 
            outside the polylinker region, yeast selection marker TRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Scutteri L, Barth P, Rahi SJ
  TITLE     Minimal integrating shuttle vectors for Saccharomyces cerevisiae 
            depleted of restriction sites outside the polylinker region
  JOURNAL   bioRxiv 2024.11.05.622133
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.05.622133"
COMMENT     Sequence Label: pLS404
FEATURES             Location/Qualifiers
     source          1..2679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(283..302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1437..1456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1537..1553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1537..1553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1610..1627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1610..1626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1627..1904
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             1905..2579
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaacagg aaacagctat gacgcgcgcg
     1561 agctcggtac ccgggatcct ctagagtcga cctgcaggca tgccctagga ctggccgtcg
     1621 ttttacaacg acattactat atatataata taggaagcat ttaatagaac agcatcgtaa
     1681 tatatgtgta ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga
     1741 aaaatagctt gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta
     1801 agaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
     1861 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttatcaatt
     1921 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgccg
     1981 aatgtgctct tgattccgat gctgacttgc tcggtattat atgtgtcccc aatagaaaga
     2041 gaactattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
     2101 gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaaccaaag gaggatgttt
     2161 tggctctggt caatgattac ggcattgata tagtgcaact gcatggtgac gagtcgtggc
     2221 aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact
     2281 gcaacatact actcagcgca gcttcacaga aacctcattc gtttattccc ttgtttgatt
     2341 cagaagcggg tgggacaggt gaacttttgg attggaactc gatttctgat tgggtaggaa
     2401 ggcaagagag tcccgaaagt ttacatttta tgttagctgg tggactgacg ccagaaaatg
     2461 ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga
     2521 caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg
     2581 ttattactga gtagtattta tttaagtatt gtttgtgcat ttgcctgcaa gccttttgaa
     2641 aagcaagcat aaaatatcta aacataaaat ctgtaaaat
//
