LOCUS       sequence-459825-       11768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding a codon-optimized L gene from 
            vesicular stomatitis virus, strain Indiana..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Marques MC, Andreu-Moreno I, Sanjuan R, Elena SF, Geller R
  TITLE     An efficient plasmid-based system for the recovery of recombinant 
            vesicular stomatitis virus encoding foreign glycoproteins.
  JOURNAL   Sci Rep. 2024 Jun 25;14(1):14644. doi: 10.1038/s41598-024-65384-8.
  PUBMED    38918479
REFERENCE   2  (bases 1 to 11768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-06-25"; volume: "14(1):14644. doi"; pages: " 
            10.1038/s41598-024-65384-8"
COMMENT     Sequence Label: pCMV-Lopt
FEATURES             Location/Qualifiers
     source          1..11768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    58..179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(69..88)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     123..142
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      365..820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(452..471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     662..683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        937..1294
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        937..1128
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(960..980)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1145..1280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1345..2139
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1399..1418)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2009..2028
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2203..2251
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2292..2311)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2411..2433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2471..2489)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2557..2661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2662..2730,2731..3522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2880..2899)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3693..4281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4182..4201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4492..4871
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4872..5083
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5034..5054
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5080..5104
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5244..5376
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5359..5378
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      5428..5437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agtcgacccg ggcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa
       61 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      121 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      181 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
      241 aaagcaagta aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg
      301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      361 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      421 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      481 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      541 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
      601 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      661 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      721 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      781 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
      841 ttacgcatct gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga
      901 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
      961 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1021 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     1141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1201 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1261 aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt
     1321 ctcgaactta aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg
     1381 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     1441 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     1501 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     1561 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     1621 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     1681 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     1741 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     1801 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     1861 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     1921 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1981 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     2041 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     2101 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     2161 tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt
     2221 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg
     2281 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     2341 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2401 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2461 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2521 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2581 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2641 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2701 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2761 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2821 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2881 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2941 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3001 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3061 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3121 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3181 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3241 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3301 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3361 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3421 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3481 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3541 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3601 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3661 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3721 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3781 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3841 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3901 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3961 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4021 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4081 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4141 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4201 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4261 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4321 tgctggcctt ttgctcacat ggctcgacag atcttcaata ttggccatta gccatattat
     4381 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     4441 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tggcattgat
     4501 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4561 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4621 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4681 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4741 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4801 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     4861 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     4921 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4981 atcaacggga ctttccaaaa tgtcgtaaca actgcgatcg cccgccccgt tgacgcaaat
     5041 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     5101 gatcactaga agctttattg cggtagttta tcacagttaa attgctaacg cagtcagtgc
     5161 ttctgacaca acagtctcga acttaagctg cagtgactct cttaaggtag ccttgcagaa
     5221 gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc
     5281 aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg
     5341 gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca
     5401 gctcttaagg ctagagtact taagctagcc accatggaag tgcacgactt cgagactgac
     5461 gagtttaatg atttcaacga ggacgattac gccacaagag agttcctgaa tccagacgag
     5521 cgaatgacgt acctgaacca cgctgattac aacctgaaca gccctctgat cagcgacgac
     5581 atcgacaatc tgatcagaaa gttcaatagc ctgcccatac cctctatgtg ggacagcaag
     5641 aactgggacg gagtcctgga aatgctcacc tcttgccagg ccaatcctat ccccacaagt
     5701 cagatgcaca agtggatggg cagttggctg atgagcgaca atcacgacgc atcccagggc
     5761 tacagcttcc tgcacgaggt ggacaaggaa gccgagatca ccttcgatgt ggtggaaacc
     5821 ttcatcagag gctggggaaa caagcctatc gagtacatca aaaaagagag atggacagac
     5881 tctttcaaga tccttgccta cctgtgccaa aagttcttgg acctgcataa gctgacactg
     5941 atcctgaacg ctgtgtctga agtggaattg ctgaacctgg cgcgaacctt taaaggcaaa
     6001 gtgcggagat ccagccatgg aaccaacatc tgcagaatca gagtgccttc tctcggccca
     6061 acctttatca gcgaaggctg ggcctacttt aagaagctgg acatcctgat ggacaggaac
     6121 ttcctgttga tggttaagga cgttatcatc ggacgcatgc agaccgtgct gagcatggtc
     6181 tgcagaatcg ataacctgtt tagcgagcaa gacatcttta gcctgctgaa catttatagg
     6241 atcggcgaca agatcgtcga aagacaggga aacttcagct acgacctgat caaaatggtg
     6301 gagcctatct gcaacctgaa gctcatgaag ctggcccggg agagcagacc tctggtcccc
     6361 cagttccctc acttcgagaa ccacatcaag acaagcgtgg acgagggcgc caagattgac
     6421 cggggtatcc ggtttctgca tgatcagatc atgagcgtga agaccgtgga cctcacgctg
     6481 gtgatttacg gaagcttcag gcactggggc caccctttca tcgattacta caccggactt
     6541 gagaagctgc actcgcaggt cacgatgaaa aaagacatcg atgtgtctta cgccaaggct
     6601 ctggcctccg acctagccag aatcgtgctg ttccagcagt tcaacgacca caagaagtgg
     6661 ttcgtgaacg gcgacctgtt gccccacgac caccccttca agtcacatgt gaaagagaac
     6721 acctggccta cagctgctca ggtgcaggac ttcggcgaca agtggcacga gctgcctctg
     6781 attaaatgct tcgagatccc cgacctgctg gacccctcca tcatctacag cgataaatcc
     6841 cactctatga accggagcga ggtgctgaag cacgtgagaa tgaaccccaa cactcccatc
     6901 ccttctaaaa aggtgctgca gaccatgctg gatacaaagg ccacaaattg gaaggaattc
     6961 ctgaaggaaa ttgacgagaa gggcctggac gacgatgacc tgataatcgg gctgaaaggc
     7021 aaggagcgcg aactgaaact ggctggccgg ttcttctctc tcatgagctg gaaactccgg
     7081 gaatacttcg tgatcaccga atatctgatc aagacccact tcgtccccat gtttaaaggc
     7141 ctgaccatgg ccgatgacct gaccgccgtg atcaagaaga tgctggatag cagcagcggg
     7201 cagggcctga aaagttacga agccatttgc atcgccaacc acatcgatta cgagaagtgg
     7261 aacaatcatc agagaaagct gagcaacggc cctgtgtttc gggtcatggg ccagttcctg
     7321 ggctatccta gcctgatcga gagaacccat gaattcttcg agaagagcct gatctattac
     7381 aacggaagac ctgacctgat gcgtgtgcac aacaacacac tgataaacag caccagccaa
     7441 agagtttgct ggcagggcca ggagggcggt ttggagggcc tccgccagaa gggctggtcc
     7501 atcctgaacc tgctggtgat ccagagagaa gccaagatcc gaaataccgc tgttaaagtg
     7561 ctggcccagg gcgataacca ggtgatctgt acccagtata agaccaagaa gagccggaat
     7621 gtggtggaac tgcagggggc cctgaatcaa atggtgtcta acaacgagaa gatcatgacc
     7681 gccatcaaga tcggcacagg caagctgggc ctcctgatca acgatgatga gacaatgcag
     7741 tccgccgact acctcaacta cgggaagatc cctattttca gaggcgtgat ccggggcctg
     7801 gaaaccaaga gatggagccg ggtcacctgt gtgaccaacg accagatccc cacctgtgcc
     7861 aatatcatgt caagcgtgag cactaacgcc ctgaccgtcg cccactttgc tgagaacccc
     7921 atcaacgcca tgatccagta caactacttc ggcacctttg cgcgcttact gctgatgatg
     7981 cacgaccccg ccctgcggca gagcctgtac gaggtccagg ataagatccc tggcctgcat
     8041 agctctacct ttaagtacgc gatgctgtac ctggacccaa gcatcggcgg cgtgtctggc
     8101 atgagcttaa gcaggttcct gattagagct ttccctgatc ctgtgacaga gagcctgtca
     8161 ttctggcggt tcatccacgt gcacgcgaga tccgagcacc tgaaggaaat gtccgccgtg
     8221 ttcggaaacc ctgagatcgc caagttccgg attacccaca tcgataagct tgtggaagat
     8281 cctactagcc tcaatattgc aatgggcatg tcgccggcaa acctgctgaa gaccgaggtg
     8341 aagaagtgcc tcatcgagtc gcgccagaca atcagaaacc aggtgataaa ggatgctaca
     8401 atttacctgt accacgagga ggatagactg cggtcttttc tgtggagtat caaccccctg
     8461 tttcctagat ttctgtccga gttcaagagc ggcacattcc tgggcgtggc tgacggactg
     8521 atctctctgt tccagaacag cagaaccatc agaaacagct ttaaaaagaa gtaccacaga
     8581 gagctagatg atctgatcgt gcggtcagag gtgagctctc tgactcacct gggaaaactg
     8641 cacttaagac ggggcagctg caagatgtgg acctgcagcg ccactcacgc cgataccctg
     8701 agatacaaaa gctggggccg gaccgtgatc ggcacgaccg tgccccaccc tctggaaatg
     8761 ctgggccctc aacacagaaa ggaaacacct tgcgcccctt gtaatacctc cggcttcaat
     8821 tacgtgagtg tgcactgccc cgacgggatc cacgacgtat tctctagcag aggccctctg
     8881 cctgcctacc tgggttccaa aaccagtgaa tccacaagca tcctgcaacc ttgggagaga
     8941 gaaagcaagg tgcccctaat caaaagggcc acaagacttc gggatgccat ctcctggttc
     9001 gtggagcccg atagcaagct ggccatgacc atcctgtcta acatccattc gcttaccggc
     9061 gaagagtgga ccaagcggca gcacggcttc aaaagaacag gctcagccct gcaccggttc
     9121 agcacatcaa gaatgagcca cggcggattc gccagccaaa gcactgccgc cctgacccgg
     9181 ctgatggcta caaccgatac catgcgggac ctgggcgatc agaatttcga tttcttgttt
     9241 caggccaccc tgctttatgc ccagatcaca acaaccgttg ccagggacgg ctggatcaca
     9301 agctgcaccg accactatca catcgcctgc aagtcttgcc tgaggccaat cgaagagatt
     9361 accctggaca gctctatgga ttacacacct cccgacgtgt cccacgtgct gaagacctgg
     9421 cggaatgggg aaggcagctg gggccaggag atcaagcaga tctaccctct ggagggaaat
     9481 tggaagaacc tggcccctgc cgaacagtcc taccaggtcg gaagatgcat cggcttcctg
     9541 tacggcgacc tggcttacag aaagtccacc cacgccgaag atagcagctt attccctctg
     9601 agtatccaag gtaggatccg aggtcgtgga ttccttaaag gcctgctgga cggcctgatg
     9661 cgggccagct gctgccaggt gattcaccgg agatctctgg ctcacctgaa gcggcctgcc
     9721 aacgccgtat acggcggcct gatctacctg atcgataagc tgagcgtgtc ccctcctttc
     9781 ctatccttga cccggagcgg accaatacgg gatgaactgg aaaccatccc gcacaaaatc
     9841 cctacctctt accctacatc caatagagat atgggcgtta tcgtgcggaa ctacttcaag
     9901 taccagtgca gattaatcga gaagggtaaa tacagaagcc actactctca gctgtggctg
     9961 ttttctgatg tgctgagcat cgattttatc ggccccttca gcatctccac cacgctgctg
    10021 cagatcctgt acaagccctt cctgtctgga aaggacaaga atgagctgcg ggagctggcc
    10081 aatctgagct ctctcctgag aagtggcgaa ggatgggagg acatccacgt taaattcttc
    10141 accaaggaca tcctgctgtg tcctgaggaa atccggcacg cctgtaaatt tggcatcgct
    10201 aaagacaaca acaaggacat gtcctaccct ccttggggca gagagagcag aggcaccatt
    10261 accacaatcc ccgtgtacta caccacaacc ccttacccca agatgctgga aatgcctcct
    10321 agaatccaga accctctgct ctcaggtata agactgggcc agctgccaac cggcgctcac
    10381 tacaagatta ggagcatcct gcacggcatg ggcatccatt acagagactt cctatcttgc
    10441 ggcgacggct ccggcggaat gactgccgcc ctgctgagag aaaacgtgca cagccggggc
    10501 atcttcaact cgttactcga attaagcggc tccgtaatga gaggcgccag ccctgaacct
    10561 ccaagcgccc ttgaaaccct gggcggcgat aagtcacggt gcgtgaacgg cgagacttgc
    10621 tgggaatacc ctagcgactt atgcgacccc cggacctggg attacttcct gcggctcaag
    10681 gcgggcttgg gcctgcagat cgatctgatt gtgatggaca tggaagtgcg agactccagc
    10741 accagcctaa agatcgaaac caacgtcaga aactacgtgc acagaatcct ggacgagcag
    10801 ggggtcctga tctataagac ctacggcacc tacatctgtg aatctgaaaa gaacgctgtg
    10861 acgatcctgg gcccaatgtt caagacagtg gatctggtgc aaacagaatt cagctccagc
    10921 cagacctcag aagtctacat ggtctgcaag ggcctcaaga agctgatcga tgaacctaac
    10981 cctgactggt cttccatcaa tgaaagctgg aagaatctgt acgccttcca gagcagcgag
    11041 caggagttcg ccagagcgaa aaaggtgtcc acctacttta cactgacagg catcccttcc
    11101 cagtttatcc ctgacccctt cgtgaacatc gagacaatgt tgcagatctt tggagtgcca
    11161 accggcgtat ctcacgctgc cgccctgaag agctcagaca gaccagccga tctgctgaca
    11221 attagccttt tctacatggc catcatctct tactacaaca tcaaccacat cagggtgggc
    11281 cctatcccac ctaaccctcc ttctgacggc atcgcacaga acgtcggcat cgccataacc
    11341 gggatcagct tctggctgag tctgatggaa aaagacatcc ccctctatca gcagtgtctg
    11401 gccgtgatcc agcagagctt ccccatccgg tgggaggccg tgagcgtgaa aggaggatac
    11461 aaacagaagt ggagcacaag aggcgacggc ctgcccaagg acacaagaat ctccgactcg
    11521 ctggccccta tcggaaactg gatcagatcc ctggaactcg tgagaaacca ggtgcggctc
    11581 aacccgttca atgaaatcct gttcaaccag ctgtgtcgga ctgtggacaa ccacctgaag
    11641 tggtcaaacc tccgcaggaa caccggaatg atcgagtgga tcaacagaag aatcagcaaa
    11701 gaggacagaa gcattctgat gctgaagtcc gacctgcacg aggagaacag ctggagagac
    11761 tgatctag
//
