LOCUS       sequence-459851-       13492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the genome of VSV driven by a T7 polymerase promoter. It 
            lacks the G protein (glycoprotein) and encodes eGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Marques MC, Andreu-Moreno I, Sanjuan R, Elena SF, Geller R
  TITLE     An efficient plasmid-based system for the recovery of recombinant 
            vesicular stomatitis virus encoding foreign glycoproteins.
  JOURNAL   Sci Rep. 2024 Jun 25;14(1):14644. doi: 10.1038/s41598-024-65384-8.
  PUBMED    38918479
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-06-25"; volume: "14(1):14644. doi"; pages: " 
            10.1038/s41598-024-65384-8"
COMMENT     Sequence Label: pVSV-Delta-G-eGFP-linker
FEATURES             Location/Qualifiers
     source          1..13492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3781..3783,3784..3786,3787..4500)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3781..3783,3784..3786,3787..4497)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3826..3847)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4087..4106)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4434..4455
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(11178..11196)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11192..11239
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(11307..11325)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11346..11362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11346..11362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11359..11381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11370..11386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11394..11400,11401..11418,11419..11424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11439..11460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11577..11594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11748..12336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11828..11847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12507..13298,13299..13367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13130..13149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13368..13472)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta taggacgaag acaaacaaac
      661 cattattatc attaaaaggc tcaggagaaa ctttaacagt aatcaaaatg tctgttacag
      721 tcaagagaat cattgacaac acagtcgtag ttccaaaact tcctgcaaat gaggatccag
      781 tggaataccc ggcagattac ttcagaaaat caaaggagat tcctctttac atcaatacta
      841 caaaaagttt gtcagatcta agaggatatg tctaccaagg cctcaaatcc ggaaatgtat
      901 caatcataca tgtcaacagc tacttgtatg gagcattaaa ggacatccgg ggtaagttgg
      961 ataaagattg gtcaagtttc ggaataaaca tcgggaaagc aggggataca atcggaatat
     1021 ttgaccttgt atccttgaaa gccctggacg gcgtacttcc agatggagta tcggatgctt
     1081 ccagaaccag cgcagatgac aaatggttgc ctttgtatct acttggctta tacagagtgg
     1141 gcagaacaca aatgcctgaa tacagaaaaa agctcatgga tgggctgaca aatcaatgca
     1201 aaatgatcaa tgaacagttt gaacctcttg tgccagaagg tcgtgacatt tttgatgtgt
     1261 ggggaaatga cagtaattac acaaaaattg tcgctgcagt ggacatgttc ttccacatgt
     1321 tcaaaaaaca tgaatgtgcc tcgttcagat acggaactat tgtttccaga ttcaaagatt
     1381 gtgctgcatt ggcaacattt ggacacctct gcaaaataac cggaatgtct acagaagatg
     1441 taacgacctg gatcttgaac cgagaagttg cagatgaaat ggtccaaatg atgcttccag
     1501 gccaagaaat tgacaaggcc gattcataca tgccttattt gatcgacttt ggattgtctt
     1561 ctaagtctcc atattcttcc gtcaaaaacc ctgccttcca cttctggggg caattgacag
     1621 ctcttctgct cagatccacc agagcaagga atgcccgaca gcctgatgac attgagtata
     1681 catctcttac tacagcaggt ttgttgtacg cttatgcagt aggatcctct gccgacttgg
     1741 cacaacagtt ttgtgttgga gataacaaat acactccaga tgatagtacc ggaggattga
     1801 cgactaatgc accgccacaa ggcagagatg tggtcgaatg gctcggatgg tttgaagatc
     1861 aaaacagaaa accgactcct gatatgatgc agtatgcgaa aagagcagtc atgtcactgc
     1921 aaggcctaag agagaagaca attggcaagt atgctaagtc agaatttgac aaatgaccct
     1981 ataattctca gatcacctat tatatattat gctacatatg aaaaaaacta acagatatca
     2041 tggataatct cacaaaagtt cgtgagtatc tcaagtccta ttctcgtctg gatcaggcgg
     2101 taggagagat agatgagatc gaagcacaac gagctgaaaa gtccaattat gagttgttcc
     2161 aagaggatgg agtggaagag catactaagc cctcttattt tcaggcagca gatgattctg
     2221 acacagaatc tgaaccagaa attgaagaca atcaaggctt gtatgcacca gatccagaag
     2281 ctgagcaagt tgaaggcttt atacaggggc ctttagatga ctatgcagat gaggaagtgg
     2341 atgttgtatt tacttcggac tggaaacagc ctgagcttga atctgacgag catggaaaga
     2401 ccttacggtt gacatcgcca gagggtttaa gtggagagca gaaatcccag tggctttcga
     2461 cgattaaagc agtcgtgcaa agtgccaaat actggaatct ggcagagtgc acatttgaag
     2521 catcgggaga aggggtcatt atgaaggagc gccagataac tccggatgta tataaggtca
     2581 ctccagtgat gaacacacat ccgtcccaat cagaagcagt atcagatgtt tggtctctct
     2641 caaagacatc catgactttc caacccaaga aagcaagtct tcagcctctc accatatcct
     2701 tggatgaatt gttctcatct agaggagagt tcatctctgt cggaggtgac ggacgaatgt
     2761 ctcataaaga ggccatcctg ctcggcctga gatacaaaaa gttgtacaat caggcgagag
     2821 tcaaatattc tctgtagact atgaaaaaaa gtaacagata tcacgatcta agtgttatcc
     2881 caatccattc atcatgagtt ccttaaagaa gattctcggt ctgaagggga aaggtaagaa
     2941 atctaagaaa ttagggatcg caccaccccc ttatgaagag gacactagca tggagtatgc
     3001 tccgagcgct ccaattgaca aatcctattt tggagttgac gagatggaca cctatgatcc
     3061 gaatcaatta agatatgaga aattcttctt tacagtgaaa atgacggtta gatctaatcg
     3121 tccgttcaga acatactcag atgtggcagc cgctgtatcc cattgggatc acatgtacat
     3181 cggaatggca gggaaacgtc ccttctacaa aatcttggct tttttgggtt cttctaatct
     3241 aaaggccact ccagcggtat tggcagatca aggtcaacca gagtatcacg ctcactgcga
     3301 aggcagggct tatttgccac ataggatggg gaagacccct cccatgctca atgtaccaga
     3361 gcacttcaga agaccattca atataggtct ttacaaggga acgattgagc tcacaatgac
     3421 catctacgat gatgagtcac tggaagcagc tcctatgatc tgggatcatt tcaattcttc
     3481 caaattttct gatttcagag agaaggcctt aatgtttggc ctgattgtcg agaaaaaggc
     3541 atctggagcg tgggtcctgg actctatcgg ccacttcaaa tgagctagtc taacttctag
     3601 cttctgaaca atccccggtt tactcagtct cccctaattc cagcctctcg aacaactaat
     3661 atcctgtctt ttctatccct atgaaaaaaa ctaacagaga tcgatctgtt tacgcgtcac
     3721 tttaattaac tcaaatcctg ctaggtatga aaaaaactaa cagatatcac gctcgagacc
     3781 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3841 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3901 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3961 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4021 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4081 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4141 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4201 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4261 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4321 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4381 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4441 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4501 gctagccaga ttcttcatgt ttggaccaaa tcaacttgtg ataccatgct caaagaggcc
     4561 tcaattatat ttgagttttt aatttttatg aaaaaaacta acagcaatca tggaagtcca
     4621 cgattttgag accgacgagt tcaatgattt caatgaagat gactatgcca caagagaatt
     4681 cctgaatccc gatgagcgca tgacgtactt gaatcatgct gattacaacc tgaattctcc
     4741 tctaattagt gatgatattg acaatttaat caggaaattc aattctcttc caattccctc
     4801 gatgtgggat agtaagaact gggatggagt tcttgagatg ttaacgtcat gtcaagccaa
     4861 tcccatccca acatctcaga tgcataaatg gatgggaagt tggttaatgt ctgataatca
     4921 tgatgccagt caagggtata gttttttaca tgaagtggac aaagaggcag aaataacatt
     4981 tgacgtggtg gagaccttca tccgcggctg gggcaacaaa ccaattgaat acatcaaaaa
     5041 ggaaagatgg actgactcat tcaaaattct cgcttatttg tgtcaaaagt ttttggactt
     5101 acacaagttg acattaatct taaatgctgt ctctgaggtg gaattgctca acttggcgag
     5161 gactttcaaa ggcaaagtca gaagaagttc tcatggaacg aacatatgca ggattagggt
     5221 tcccagcttg ggtcctactt ttatttcaga aggatgggct tacttcaaga aacttgatat
     5281 tctaatggac cgaaactttc tgttaatggt caaagatgtg attataggga ggatgcaaac
     5341 ggtgctatcc atggtatgta gaatagacaa cctgttctca gagcaagaca tcttctccct
     5401 tctaaatatc tacagaattg gagataaaat tgtggagagg cagggaaatt tttcttatga
     5461 cttgattaaa atggtggaac cgatatgcaa cttgaagctg atgaaattag caagagaatc
     5521 aaggccttta gtcccacaat tccctcattt tgaaaatcat atcaagactt ctgttgatga
     5581 aggggcaaaa attgaccgag gtataagatt cctccatgat cagataatga gtgtgaaaac
     5641 agtggatctc acactggtga tttatggatc gttcagacat tggggtcatc cttttataga
     5701 ttattacact ggactagaaa aattacattc ccaagtaacc atgaagaaag atattgatgt
     5761 gtcatatgca aaagcacttg caagtgattt agctcggatt gttctatttc aacagttcaa
     5821 tgatcataaa aagtggttcg tgaatggaga cttgctccct catgatcatc cctttaaaag
     5881 tcatgttaaa gaaaatacat ggcccacagc tgctcaagtt caagattttg gagataaatg
     5941 gcatgaactt ccgctgatta aatgttttga aatacccgac ttactagacc catcgataat
     6001 atactctgac aaaagtcatt caatgaatag gtcagaggtg ttgaaacatg tccgaatgaa
     6061 tccgaacact cctatcccta gtaaaaaggt gttgcagact atgttggaca caaaggctac
     6121 caattggaaa gaatttctta aagagattga tgagaagggc ttagatgatg atgatctaat
     6181 tattggtctt aaaggaaagg agagggaact gaagttggca ggtagatttt tctccctaat
     6241 gtcttggaaa ttgcgagaat actttgtaat taccgaatat ttgataaaga ctcatttcgt
     6301 ccctatgttt aaaggcctga caatggcgga cgatctaact gcagtcatta aaaagatgtt
     6361 agattcctca tccggccaag gattgaagtc atatgaggca atttgcatag ccaatcacat
     6421 tgattacgaa aaatggaata accaccaaag gaagttatca aacggcccag tgttccgagt
     6481 tatgggccag ttcttaggtt atccatcctt aatcgagaga actcatgaat tttttgagaa
     6541 aagtcttata tactacaatg gaagaccaga cttgatgcgt gttcacaaca acacactgat
     6601 caattcaacc tcccaacgag tttgttggca aggacaagag ggtggactgg aaggtctacg
     6661 gcaaaaagga tggagtatcc tcaatctact ggttattcaa agagaggcta aaatcagaaa
     6721 cactgctgtc aaagtcttgg cacaaggtga taatcaagtt atttgcacac agtataaaac
     6781 gaagaaatcg agaaacgttg tagaattaca gggtgctctc aatcaaatgg tttctaataa
     6841 tgagaaaatt atgactgcaa tcaaaatagg gacagggaag ttaggacttt tgataaatga
     6901 cgatgagact atgcaatctg cagattactt gaattatgga aaaataccga ttttccgtgg
     6961 agtgattaga gggttagaga ccaagagatg gtcacgagtg acttgtgtca ccaatgacca
     7021 aatacccact tgtgctaata taatgagctc agtttccaca aatgctctca ccgtagctca
     7081 ttttgctgag aacccaatca atgccatgat acagtacaat tattttggga catttgctag
     7141 actcttgttg atgatgcatg atcctgctct tcgtcaatca ttgtatgaag ttcaagataa
     7201 gataccgggc ttgcacagtt ctactttcaa atacgccatg ttgtatttgg acccttccat
     7261 tggaggagtg tcgggcatgt ctttgtccag gtttttgatt agagccttcc cagatcccgt
     7321 aacagaaagt ctctcattct ggagattcat ccatgtacat gctcgaagtg agcatctgaa
     7381 ggagatgagt gcagtatttg gaaaccccga gatagccaag tttcgaataa ctcacataga
     7441 caagctagta gaagatccaa cctctctgaa catcgctatg ggaatgagtc cagcgaactt
     7501 gttaaagact gaggttaaaa aatgcttaat cgaatcaaga caaaccatca ggaaccaggt
     7561 gattaaggat gcaaccatat atttgtatca tgaagaggat cggctcagaa gtttcttatg
     7621 gtcaataaat cctctgttcc ctagattttt aagtgaattc aaatcaggca cttttttggg
     7681 agtcgcagac gggctcatca gtctatttca aaattctcgt actattcgga actcctttaa
     7741 gaaaaagtat catagggaat tggatgattt gattgtgagg agtgaggtat cctctttgac
     7801 acatttaggg aaacttcatt tgagaagggg atcatgtaaa atgtggacat gttcagctac
     7861 tcatgctgac acattaagat acaaatcctg gggccgtaca gttattggga caactgtacc
     7921 ccatccatta gaaatgttgg gtccacaaca tcgaaaagag actccttgtg caccatgtaa
     7981 cacatcaggg ttcaattatg tttctgtgca ttgtccagac gggatccatg acgtctttag
     8041 ttcacgggga ccattgcctg cttatctagg gtctaaaaca tctgaatcta catctatttt
     8101 gcagccttgg gaaagggaaa gcaaagtccc actgattaaa agagctacac gtcttagaga
     8161 tgctatctct tggtttgttg aacccgactc taaactagca atgactatac tttctaacat
     8221 ccactcttta acaggcgaag aatggaccaa aaggcagcat gggttcaaaa gaacagggtc
     8281 tgcccttcat aggttttcga catctcggat gagccatggt gggttcgcat ctcagagcac
     8341 tgcagcattg accaggttga tggcaactac agacaccatg agggatctgg gagatcagaa
     8401 tttcgacttt ttattccaag caacgttgct ctatgctcaa attaccacca ctgttgcaag
     8461 agacggatgg atcaccagtt gtacagatca ttatcatatt gcctgtaagt cctgtttgag
     8521 acccatagaa gagatcaccc tggactcaag tatggactac acgcccccag atgtatccca
     8581 tgtgctgaag acatggagga atggggaagg ttcgtgggga caagagataa aacagatcta
     8641 tcctttagaa gggaattgga agaatttagc acctgctgag caatcctatc aagtcggcag
     8701 atgtataggt tttctatatg gagacttggc gtatagaaaa tctactcatg ccgaggacag
     8761 ttctctattt cctctatcta tacaaggtcg tattagaggt cgaggtttct taaaagggtt
     8821 gctagacgga ttaatgagag caagttgctg ccaagtaata caccggagaa gtctggctca
     8881 tttgaagagg ccggccaacg cagtgtacgg aggtttgatt tacttgattg ataaattgag
     8941 tgtatcacct ccattccttt ctcttactag atcaggacct attagagacg aattagaaac
     9001 gattccccac aagatcccaa cctcctatcc gacaagcaac cgtgatatgg gggtgattgt
     9061 cagaaattac ttcaaatacc aatgccgtct aattgaaaag ggaaaataca gatcacatta
     9121 ttcacaatta tggttattct cagatgtctt atccatagac ttcattggac cattctctat
     9181 ttccaccacc ctcttgcaaa tcctatacaa gccattttta tctgggaaag ataagaatga
     9241 gttgagagag ctggcaaatc tttcttcatt gctaagatca ggagaggggt gggaagacat
     9301 acatgtgaaa ttcttcacca aggacatatt attgtgtcca gaggaaatca gacatgcttg
     9361 caagttcggg attgctaagg ataataataa agacatgagc tatccccctt ggggaaggga
     9421 atccagaggg acaattacaa caatccctgt ttattatacg accacccctt acccaaagat
     9481 gctagagatg cctccaagaa tccaaaatcc cctgctgtcc ggaatcaggt tgggccaatt
     9541 accaactggc gctcattata aaattcggag tatattacat ggaatgggaa tccattacag
     9601 ggacttcttg agttgtggag acggctccgg agggatgact gctgcattac tacgagaaaa
     9661 tgtgcatagc agaggaatat tcaatagtct gttagaatta tcagggtcag tcatgcgagg
     9721 cgcctctcct gagcccccca gtgccctaga aactttagga ggagataaat cgagatgtgt
     9781 aaatggtgaa acatgttggg aatatccatc tgacttatgt gacccaagga cttgggacta
     9841 tttcctccga ctcaaagcag gcttggggct tcaaattgat ttaattgtaa tggatatgga
     9901 agttcgggat tcttctacta gcctgaaaat tgagacgaat gttagaaatt atgtgcaccg
     9961 gattttggat gagcaaggag ttttaatcta caagacttat ggaacatata tttgtgagag
    10021 cgaaaagaat gcagtaacaa tccttggtcc catgttcaag acggtcgact tagttcaaac
    10081 agaatttagt agttctcaaa cgtctgaagt atatatggta tgtaaaggtt tgaagaaatt
    10141 aatcgatgaa cccaatcccg attggtcttc catcaatgaa tcctggaaaa acctgtacgc
    10201 attccagtca tcagaacagg aatttgccag agcaaagaag gttagtacat actttacctt
    10261 gacaggtatt ccctcccaat tcattcctga tccttttgta aacattgaga ctatgctaca
    10321 aatattcgga gtacccacgg gtgtgtctca tgcggctgcc ttaaaatcat ctgatagacc
    10381 tgcagattta ttgaccatta gcctttttta tatggcgatt atatcgtatt ataacatcaa
    10441 tcatatcaga gtaggaccga tacctccgaa ccccccatca gatggaattg cacaaaatgt
    10501 ggggatcgct ataactggta taagcttttg gctgagtttg atggagaaag acattccact
    10561 atatcaacag tgtttagcag ttatccagca atcattcccg attaggtggg aggctgtttc
    10621 agtaaaagga ggatacaagc agaagtggag tactagaggt gatgggctcc caaaagatac
    10681 ccgaatttca gactccttgg ccccaatcgg gaactggatc agatctctgg aattggtccg
    10741 aaaccaagtt cgtctaaatc cattcaatga gatcttgttc aatcagctat gtcgtacagt
    10801 ggataatcat ttgaaatggt caaatttgcg aagaaacaca ggaatgattg aatggatcaa
    10861 tagacgaatt tcaaaagaag accggtctat actgatgttg aagagtgacc tacacgagga
    10921 aaactcttgg agagattaaa aaatcatgag gagactccaa actttaagta tgaaaaaaac
    10981 tttgatcctt aagaccctct tgtggttttt attttttatc tggttttgtg gtcttcgtgg
    11041 gtcggcatgg catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgtcgt
    11101 ccactcggat ggctaaggga ggggcccccg cggggctgct aacaaagccc gaaaggaagc
    11161 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
    11221 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatcgagac ctcgatacta
    11281 gtgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
    11341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    11401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    11461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    11581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    12421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    12661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    13021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    13081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    13141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    13201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    13261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    13441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gc
//
