LOCUS       sequence-459907-        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
COMMENT     Sequence Label: phND2-N174
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2571..3365)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2571..3365)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2682..2701)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3292..3311
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3392..3891)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3440..3459)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3833..3856
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     intron          5108..6046
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5131..5151)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(6047..6258)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6336..6451
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(6714..6758)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6943..7176
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7673..7798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7845..8478
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8646..8780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8670..8689)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8724..8743
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8828..8932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8933..9001,9002..9793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9151..9170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgc cccgcccggg tcagaagaac
     2581 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     2641 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     2701 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     2761 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     2821 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     2881 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     2941 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     3001 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     3061 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     3121 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     3181 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     3241 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     3301 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     3361 atcatggtcc taggaagctt gggctgcagg tcgaaaggcc cggagatgag gaagaggaga
     3421 acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc
     3481 gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca
     3541 gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta
     3601 cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt
     3661 ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg
     3721 gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg
     3781 tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg
     3841 ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt
     3901 cacgcgttcg cgatctagag ccggcgcggc ctcagttatg aaaaaacgca ttgagctcct
     3961 cgtacatggg gccccggtcg tggtgaaggt gcatatcgta agacaagaga ttctccgagt
     4021 ggacgccccc gcgcacagcc gacgagccga agactagccc gtggcccgtg ggccgcgaac
     4081 cgggcagcgc cgagtagtgc atagagtagt ggtagctttt ctcgtggtcg ggcgaggagt
     4141 cctgcttgag tgagaagttg ccattgagac agagcggggg gctgagcggg ccctcgtact
     4201 cggagctgtt gtagtccggg ctcgcgccgc caccgcctgc cgccgcatac agcgtctcgt
     4261 aggctgcgca gtagccgtgg gtccgcaggg cgtgcgccgc gccgccgccc aggccgccgg
     4321 ccgcctggca ctgtgcgccc gccaggcgcg agcacgggta cgggtagggg tgcatggcga
     4381 acgggccgcc cgagccgtgg aagcggccgg caccgtcggc gccttgctcc gtgaggaagt
     4441 tgcgagagtt gagctgcaga cagccggcca ccagattggt ggtgggctgc gacagaccct
     4501 tgcacagagt ctgcacgtag gacactaggt ctggccgctt gccggagcgc aggatctccg
     4561 agagcgccca gatatagttc ttggctaggc gcagcgtctc gatcttggac agcttctgcg
     4621 tcttggagta gcagggcacc accttgcgca ggttgtccag ggctgcgttc aggtcgtgca
     4681 tgcggttgcg ctcccgcgcg ttcgccttct gccgccgaag cttggagcgc tccaagcgcg
     4741 ccttggtcat cttgcgcttc ttgggcccgc ccttcttggg ccgctcgccc tccgcctcgt
     4801 ccagtccttc ttcctcctcc tcttcctcct cctcctctcc ccccagctcg ccttcctcct
     4861 tgacctcggc caacgtggcc tccgtcccct cttctccacg gagagggact ggcttggccg
     4921 cccgggctgg ccccggagcc cctggcccgg gcgcaggcgg tggcggtggc ggcgcgtcgc
     4981 ccttgtcgct cctcggctcg tcgtcttcgc cgtcgcccca gctggcgaac ttgggcacgt
     5041 ccgagagaag gccgggctcg ctgaacaggc gggtcagcat ggtggcggcc gcggatcctc
     5101 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     5161 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     5221 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     5281 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     5341 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     5401 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag
     5461 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     5521 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     5581 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     5641 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     5701 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     5761 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     5821 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     5881 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     5941 cgggatcaag aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat
     6001 aacccgtaaa gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg
     6061 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     6121 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     6181 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
     6241 gcgatgtgcg ctctgcccac tgacgggcac ctcgagtgaa gacgaaaggg cctcatcgat
     6301 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
     6361 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
     6421 tgtacccccc aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat
     6481 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     6541 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     6601 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     6661 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     6721 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     6781 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     6841 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     6901 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     6961 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     7021 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     7081 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     7141 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     7201 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     7261 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     7321 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     7381 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     7441 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     7501 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     7561 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     7621 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     7681 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     7741 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     7801 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     7861 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     7921 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     7981 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag
     8041 cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc
     8101 tccctggaaa gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga
     8161 agtactccgg atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc
     8221 actctaacac ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc
     8281 tggtgttctc tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt
     8341 agcaccatcc aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat
     8401 cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg
     8461 agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt
     8521 taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg
     8581 gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc
     8641 cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     8701 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     8761 taagctgcaa taaacaagtt gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     8821 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8881 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8941 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9001 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9061 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9121 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9841 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9961 ttc
//
