LOCUS       sequence-459923-        9190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression vector of MBD1 with a deletion of CXXC3 domain.
            C-terminal tagged to the intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   n/a
  TITLE     MBD2 modulates heterchromatin architecture and composition
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTYB1-MBD1∆CxxC3 pc4783
FEATURES             Location/Qualifiers
     source          1..9190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1765..2583
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             3039..3050
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3160..3315
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(3380..3398)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3394..3441
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3501..3605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3606..3674,3675..4466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3824..3843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4508..5021)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4703..4724)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4915..4934
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5132..5720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5621..5640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5874..5891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5913..5932)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6073..6095
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6089..6280)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6837..7919)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7881..7900
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7920..7997)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      8150..8236
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8333..8419
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8516..8602
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8699..8785
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8882..8968
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     9103..9122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9103..9121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9122..9146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9161..9183
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgatggctg agtcctggca ggactgccca gccctgggcc ctggctggaa acgccgagag
       61 tcctttcgaa agtcaggggc ctcgtttgga cgctcagaca tctattacca gagccccaca
      121 ggagaaaaga tccgaagcaa agttgagctg actcggtact tgggccctgc atgtgatctt
      181 accctgtttg acttcagaca aggcacctta tgccatccca ttcccaagac tcatcccttg
      241 gctgtcccca gcaagaagaa aaagaagcct tctaaaccag ccaagactaa gaaacagcag
      301 gttgggctgc agcgaagtga ggtcaggata gagactccac agggggaata taaggcccct
      361 actgccacag ctctagcttc actatctgtg tctgcatctg cgtcttcgtc tgcatctgca
      421 tctgcatctg cgtcttcaca tgcacctgtg tgctgtgaga attgtggaat ccacttttca
      481 tgggatggtg tcaaaaggca gagacttaag acgttgtgca aagattgtcg agcacagaga
      541 atcgccttca accgcgaaca gagaatgttt aagcgagtcg gctgtgggga ctgtgcagct
      601 tgcctggtga aagaggactg tggggtgtgc tccacctgtc gcctgcaact gcccagtgat
      661 gtggcctcag ggctttactg caagtgtgag cggagacgat gcctgcggat tatggagaag
      721 agccgagggt gtggcgtgtg tcggggttgt cagacccaag aggactgtgg ccattgctgc
      781 atctgtcttc gctctccccg ccctggtctc aagcgccagt ggaggtgttt gcaacggcgc
      841 tgtttttggg gtaaacgtga ttctagtaag agaggctcga aggtagcgtc tcagcgtcac
      901 tcccaagccc cgccacttcc tccacatcct gcatcacagt acacagagcc cacagagctg
      961 cacatcagcg acatagcacc cacatcacct gctgagttca tctattactg tgtagacgag
     1021 gacgaggacg agctaaagcg gctgctgccc agtgctgggt cagggtctgg ggagggagca
     1081 ggattgcgtc catatcagac ccatcagacc catcaaaaga ggcctgcttc tgctcgacaa
     1141 ctccaactga gctctccctt aaaggcccct tgggctgtgg tcacagcacc accaggccct
     1201 gtccaagatt caagaaagca gcaagcaggt agaggctctg tactgcccca gcctgacaca
     1261 gactttgtgt ttttacaaga gggtgccagc agtgccatgc agatgcctgg cactgctgca
     1321 gcttccacag aagtcccagt acaggcggcc cagtgctctg ccccgagctg ggttgtggcc
     1381 ttaccccagg tgaagcaaga gacggcagat gctccagagg agtggacagc agtcacgacc
     1441 ttcctgactt cctccacgct gcagtctggc ttccctagca aggctgcaga tccagacctt
     1501 ccacctgtga aacaagagcc ccctggccct gaagaggatg gagaagagaa aaaagatgat
     1561 gtctctgaaa caaccccagc agaggagata ggaggggttg gcacgccagt gatcacggag
     1621 attttcagcc tgggtggaac ccgcctccga gatgcagaag cctggttgcc aaggctacat
     1681 aaactgttag cagtaaatga aaaagagtat tttactgagc tgcagttgaa ggaggaagtt
     1741 ttggaattcc tcgagggctc ttcctgcttt gccaagggta ccaatgtttt aatggcggat
     1801 gggtctattg aatgtattga aaacattgag gttggtaata aggtcatggg taaagatggc
     1861 agacctcgtg aggtaattaa attgcccaga ggaagagaaa ctatgtacag cgtcgtgcag
     1921 aaaagtcagc acagagccca caaaagtgac tcaagtcgtg aagtgccaga attactcaag
     1981 tttacgtgta atgcgaccca tgagttggtt gttagaacac ctcgtagtgt ccgccgtttg
     2041 tctcgtacca ttaagggtgt cgaatatttt gaagttatta cttttgagat gggccaaaag
     2101 aaagcccccg acggtagaat tgttgagctt gtcaaggaag tttcaaagag ctacccaata
     2161 tctgaggggc ctgagagagc caacgaatta gtagaatcct atagaaaggc ttcaaataaa
     2221 gcttattttg agtggactat tgaggccaga gatctttctc tgttgggttc ccatgttcgt
     2281 aaagctacct accagactta cgctccaatt ctttatgaga atgaccactt tttcgactac
     2341 atgcaaaaaa gtaagtttca tctcaccatt gaaggtccaa aagtacttgc ttatttactt
     2401 ggtttatgga ttggtgatgg attgtctgac agggcaactt tttcggttga ttccagagat
     2461 acttctttga tggaacgtgt tactgaatat gctgaaaagt tgaatttgtg cgccgagtat
     2521 aaggacagaa aagaaccaca agttgccaaa actgttaatt tgtactctaa agttgtcaga
     2581 ggtaatggta ttcgcaataa tcttaatact gagaatccat tatgggacgc tattgttggc
     2641 ttaggattct tgaaggacgg tgtcaaaaat attccttctt tcttgtctac ggacaatatc
     2701 ggtactcgtg aaacatttct tgctggtcta attgattctg atggctatgt tactgatgag
     2761 catggtatta aagcaacaat aaagacaatt catacttctg tcagagatgg tttggtttcc
     2821 cttgctcgtt ctttaggctt agtagtctcg gttaacgcag aacctgctaa ggttgacatg
     2881 aatgtcacca aacataaaat tagttatgct atttatatgt ctggtggaga tgttttgctt
     2941 aacgttcttt cgaagtgtgc cggctctaaa aaattcaggc ctgctcccgc cgctgctttt
     3001 gcacgtgagt gccgcggatt ttatttcgag ttacaagaat tgaaggaaga cgattattat
     3061 gggattactt tatctgatga ttctgatcat cagtttttgc ttggatccca ggttgtcgtc
     3121 catgcatgcg gtggcctgac cggtctgaac tcaggcctca cgacaaatcc tggtgtatcc
     3181 gcttggcagg tcaacacagc ttatactgcg ggacaattgg tcacatataa cggcaagacg
     3241 tataaatgtt tgcagcccca cacctccttg gcaggatggg aaccatccaa cgttcctgcc
     3301 ttgtggcagc ttcaatgact gcaggaaggg gatccggctg ctaacaaagc ccgaaaggaa
     3361 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     3421 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggataact acgtcaggtg
     3481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3601 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3661 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3721 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3781 gccccgaaga acgtttccca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3841 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3901 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3961 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4021 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4081 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4141 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4201 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4261 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4321 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4381 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4441 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4501 ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg tataagcaaa
     4561 tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4621 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagcc
     4681 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4741 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4801 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4861 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4921 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4981 caccacaccc gccgcgctta atgcgccgct acagggcgcg taaaaggatc taggtgaaga
     5041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5881 cagtgagcga ggaagctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5941 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     6001 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6061 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6121 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct
     6181 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     6241 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     6301 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     6361 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     6421 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagccgaac gccagcaaga
     6481 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     6541 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     6601 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     6661 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     6721 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     6781 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     6841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6901 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     6961 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     7021 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     7081 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     7141 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     7201 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     7261 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     7321 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     7381 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     7441 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     7501 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     7561 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     7621 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     7681 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     7741 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     7801 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     7861 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     7921 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     7981 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     8041 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgccg
     8101 gcatgccgcc ctttcgtctt caagaattaa ttcccaattc cccaggcatc aaataaaacg
     8161 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     8221 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     8281 gtggcgggca ggacgcccgc cataaactgc caggaattaa ttccccaggc atcaaataaa
     8341 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     8401 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     8461 agggtggcgg gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat
     8521 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     8581 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     8641 cggagggtgg cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca
     8701 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     8761 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     8821 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca
     8881 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     8941 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     9001 acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt ggggatcgga
     9061 attaattccc ggtttaaacc ggggatctcg atcccgcgaa attaatacga ctcactatag
     9121 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
     9181 agatatacat
//
