LOCUS       sequence-459924-       11502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing 
            construct into the C. neoformans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11502)
  TITLE     C. neoformans AID2 degron system plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHM2642
FEATURES             Location/Qualifiers
     source          1..11502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1963..1982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1963..1981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2798..3598
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2858..2877)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3468..3487
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             6819..6872
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      7128..7137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8684..8700)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8685..8701)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(8729..8747)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8768..8784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8768..8784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8781..8803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8792..8808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8816..8822,8823..8840,8841..8846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8861..8882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8999..9016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9170..9758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9250..9269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9929..10720,10721..10789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10552..10571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10790..10894)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10931..11434
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     11475..11493
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     1981 ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctgtgcatgc aggattcgag
     2041 tggcatggtg tgcactgagt gtatggttgt cggaggagag gatgatggta acaacaatag
     2101 cagcaacgtc actcgacgcg cgtccggtgt gccacacggg gtaacgccga gtcgccgtca
     2161 gggtcgccga gaccactctc acagcgtcac cgttggcacc agctcagctt acagcttcta
     2221 tcctccgcca gcatccacat acatccccta taccgcatcc cccacccact gcccaaggtg
     2281 agtcatcttc ccgccccctt cccttgcccg ccactcagtc ctccatcctc cactaatcca
     2341 ccttatcgca cccaccgcct atcgcacatc cgagcacaat gctgggcctg ccaggggctg
     2401 ctagatggtg ctctccccac gctgatctgc atgccggcca ttggatcatg ggtgctaggt
     2461 gctgggtgct ggatgttgga tgctggatgc tgggtgcacg cttggtcatt tccttccagg
     2521 attgacggtc gccgagagga cgacgtggcg ttcgacaacg agggccgata gcaccgcatc
     2581 gcctcgacct gcatccatct cgccttgtcc ttttggtgca acaatccatc cgtgctggtg
     2641 ccacacgcat agctggaaga gatggatgtg cgttgaacag agctgccgtc aggacttttt
     2701 ggtgcacgga ccctattgtc ctccccaatc ttcaccgcgt ctcctaatat gcagcctctt
     2761 tgctaattgt ctttttccat tagtaaactc gcccaacatg tctatgattg aacaagatgg
     2821 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     2881 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     2941 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     3001 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3061 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatccca
     3121 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3181 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3241 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3301 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     3361 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3421 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3481 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3541 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagt
     3601 gaaggcggta aggggttaat tttccttaga gggtgtatat atacatatta gagaagtgat
     3661 acaattttag cacactgcga attcgagaca gacatcgtgt caatcatctt ttttgacatt
     3721 atatgcccat taatctatct acagacaaca ataccatcct tcccaccctc agcaacgccg
     3781 ttgaatcctc aggatcttca tggccattct atgctatacg gtacaagatt gtggctacta
     3841 tgcaagctcc atagcgaatg atcttacact gaccttgtcg accgttctga atgattaaca
     3901 atggtcgcaa gtgtggttga cccacctgcg ggcgcttctt aactatctat catcggtatg
     3961 tcaagtatca ctccctttaa acaaaagaat aagagcataa tacagcagtc tcatcatggc
     4021 aacccttcgc gcaatgtaca aaaagcagcc agaagccccc acgcacaagc aataatgtcg
     4081 tgttctaccc tgccatgaac aagcgtcatg gccattagcg aacgccccaa tattcagccc
     4141 cattcgccaa gcaagttgcc tcgccccctt cccagaagat catgggcatc tcgtcttctt
     4201 ctaatttcgc attttgtgaa gctccattca tgcacgtcgg cttctggacg actcttatca
     4261 tatgcattgg cctgctcggt gtgaggatga gccactccca catgtttaga cggcaggggc
     4321 ctcttgccac ccgttgaatt tttggtgcga agacgtgcct gggaacgacc tccactttaa
     4381 atttctccgt tgttgcggtc gctccatcct cctcacattt cttgcctcct aagtcccatg
     4441 tgccacactc ggtgaaaaac ggagtttgat acggatgaaa tttccagtga gcttttcaca
     4501 ttctggaggt ttcgaaaaaa attgtgggat tttctgaatt aatccgagaa tccgccctgc
     4561 ccatcaaccg gaaagaatta aatccctcgc acattccttt ctcccaccac cttctttatc
     4621 tctacattct ttaaccaaaa cacctaggtg agtacccaaa gtcttttgac acctttgaaa
     4681 atccgcgcca gtcgtagggc acgtaaaaca attctcgctg gtggcttctg caggtgaagt
     4741 cggggcagag tggagctgag catggtcgac cacccacatc attctgtctt gtttttggcg
     4801 atgtcttcca tgcttttgcc ctcatctgtt gcgtcgactg cagagtactg ttttgccact
     4861 actgagctca acatgggctt gtgtgtggtg aagaaaaaaa tttggtggct cttgaaaatt
     4921 ttcaacccat cggatacatg cccttccaac tcacaacgcc ctaacacttt attgtcctat
     4981 cattgcctct tcgcacatgc taacgatctc cacagaaaac ttcaaaatga cctacttccc
     5041 cgaggaggtc gtcgagcaca tcttctcctt cctccccgcc cagcgagacc gaaacaccgt
     5101 ctccctcgtc tgtaaggtct ggtacgagat cgagcgactc tcccgacgag gtgtcttcgt
     5161 cggtaactgt tacgccgtcc gagccggtcg agtcgccgcc cgattcccca acgtccgagc
     5221 cctcaccgtc aagggtaagc cccacggtgc cgacttcaac ctcgtccccc ccgactgggg
     5281 tggttacgcc ggtccctgga tcgaggccgc cgcccgaggt tgtcacggtc tcgaggagct
     5341 ccgaatgaag cgaatggtcg tctccgacga gtccctcgag ctcctcgccc gatccttccc
     5401 ccgattccga gccctcgtcc tcatctcctg tgagggtttc tccaccgacg gtctcgccgc
     5461 cgtcgcctcc gtatgtaacc ccgtttcccg caacatgtta ctccactaac atctcccgac
     5521 agcactgtaa gctcctccga gagctcgacc tccaggagaa cgaggtcgag gaccgaggtc
     5581 cccgatggct ctcctgtttc cccgactcct gtacctccct cgtctccctc aacttcgcct
     5641 gtatcaaggg tgaggtcaac gccggttccc tcgagcgact cgtctcccga tcccccaacc
     5701 tccgatccct ccgactcaac cgatccgtct ccgtcgacac cctcgccaag atcctcctcc
     5761 gaacccccaa cctcgaggac ctcggtaccg gtaacctcac cgacgacttc cagaccgagt
     5821 cctacttcaa gctcacctcc gccctcgaga agtgtaagat gctccgatcc ctctccggtt
     5881 tctgggacgc ctcccccgtc tgtctctcct tcatctaccc cctctgtgcc cagctcaccg
     5941 gtctcaacct ctcctacgcc cccaccctcg acgcctccga cctcaccaag atgatctccc
     6001 gatgtgtcaa gctccagcga ctctgggtcc tcgactgtat ctccgacaag ggtctccagg
     6061 tcgtcgcctc ctcctgtaag gacctccagg agctccgagt cttcccctcc gacttctacg
     6121 tcgccggtta ctccgccgtc accgaggagg gtctcgtcgc cgtctccctc ggttgtccca
     6181 agctcaactc cctcctctac ttctgtcacc agatgaccaa cgccgccctc gtcaccgtcg
     6241 ccaagaactg tcccaacttc acccgattcc gactctgtat cctcgagccc ggtaagcccg
     6301 acgtcgtcac ctcccagccc ctcgacgagg gtttcggtgc catcgtccga gagtgtaagg
     6361 gtctccagcg actctccatc tccggtctcc tcaccgacaa ggtcttcatg tacatcggta
     6421 agtacgccaa gcagctcgag atgctctcca tcgccttcgc cggtgactcc gacaagggta
     6481 tgatgcacgt catgaacggt tgtaagaacc tccgaaagct cgagatccga gactccccct
     6541 tcggtgacgc cgccctcctc ggtaacttcg cccgatacga gaccatgcga tccctctgga
     6601 tgtcctcctg taacgtcacc ctcaagggtt gtcaggtcct cgcctccaag atgcccatgc
     6661 tcaacgtcga ggtcatcaac gagcgagacg gttccaacga gatggaggag aaccacggtg
     6721 acctccccaa ggtcgagaag ctctacgtct accgaaccac cgccggtgcc cgagacgacg
     6781 cccccaactt cgtcaagatc ctcagatcag gaagcggaga gggtcgaggt tccctcctca
     6841 cctgtggtga cgtcgaggag aaccccggtc ccatggtctc caagggtgag gaggacaaca
     6901 tggcctccct ccccgccacc cacgagctcc acatcttcgg ttccatcaac ggtgtcgact
     6961 tcgacatggt cggtcagggt accggtaacc ccaacgacgg ttacgaggag ctcaacctca
     7021 agtccaccaa gggtgacctc cagttctccc cctggatcct cgtcccccac atcggttacg
     7081 gtttccacca gtacctcccc taccccgacg gtatgtcccc cttccaggcc gccatggtcg
     7141 acggttccgg ttaccaggtc caccgaacca tgcagttcga ggacggtgcc tccctcaccg
     7201 tcaactaccg atacacctac gagggttccc acatcaaggg tgaggcccag gtcaagggta
     7261 ccggtttccc cgccgacggt cccgtcatga ccaactccct caccgccgcc gactggtgtc
     7321 gatccaagaa gacctacccc aacgacaaga ccatcatctc caccttcaag tggtcctaca
     7381 ccaccggtaa cggtaagcga taccgatcca ccgcccgaac cacctacacc ttcgccaagc
     7441 ccatggccgc caactacctc aagaaccagc ccatgtacgt cttccgaaag accgagctca
     7501 agcactccaa gaccgagctc aacttcaagg agtggcagaa ggccttcacc gacgtcatgg
     7561 gtatggacga gctctacaag ggttctggat ccggaggtag tggtttctcc gagaccgtcg
     7621 acctcatgct caacctccag tccaacaagg agggttccgt cgacctcaag aacgtctccg
     7681 ccgtccccaa ggagaagacc accctcaagg acccctccaa gccccccgcc aaggcccagg
     7741 tcgtcggttg gccccccgtc cgaaactacc gaaagaacat gatgacccag cagaagacct
     7801 cctcctaatt acttatgtag tgacccttct tacgcgtaat atgcatcgga tgactgttgc
     7861 tacttgtgct tcttacgttt tgtgtcttat ttgctgatgt atacctctgc gcttcaaaat
     7921 cttcattgcc gtgatatatc attccatgtc gtttgtccgt tattggattc ccgatagttg
     7981 tccgcgaaat cgaatatttc tcggctgacg acgtctcttg caacaataaa atggccatca
     8041 ctgttgaaat ttgcacgaga gctataactt ggaataacct tcgttgctgc tttattttag
     8101 tgctttcaac tatttactca ttctcaggtc gccaccatcg cattgatatc gttccttcca
     8161 tccttccttt cctttagtcg gtcacgtctt ccttaatccg tcactccttt tttcagtcgt
     8221 tcctggagat caccacgaaa taatctcgac atgcactgac catctcggtt ctgtggactg
     8281 tcatgctaag agagttcatg ttgtaataat agtttgttgc atggaaagaa cggggaaaga
     8341 agcaagaaac agatgtacag aattaccgat gtctctacgc atccctgacc tcgtccttcc
     8401 taccgcaacc gcacttccac ctgctcacac gtcctcgtta atgccttgaa tctcagccac
     8461 acacttacct cgcctcgacg cttgtcgggc cagggcaccc ccttcatcaa cctgggaaga
     8521 gttggcgggg caaacggagg agggatgggg caggaagagg gggatggcgc acgtcgagtg
     8581 gatttggaag aaaaggggtt ggtcgaggtg gaagtcgaag cgaagatgtt gaatgtcatg
     8641 tttgggaacg ggggatcttt agaagactat gagattgaac gatcgatacc gtcgacctcg
     8701 agggggggcc cggtaccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     8761 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8941 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9001 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9061 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9601 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9901 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9961 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10021 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10081 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10141 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10201 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10261 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10321 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10381 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10441 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10501 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10561 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10621 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10681 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10741 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10921 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
    10981 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
    11041 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
    11101 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
    11161 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
    11221 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
    11281 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
    11341 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
    11401 atacgaggcg cgtgtaagtt acaggcaagc gatccgtcta agaaaccatt attatcatga
    11461 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
