LOCUS       sequence-459942-       15390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the production of SARS-CoV-2 virus-like particles (VLPs) in the 
            '2-plasmid' system and packaging firefly luciferase mRNA into the 
            VLPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15390)
  AUTHORS   Yang Q, Kelkar A, Manicassamy B, Neelamegham S
  TITLE     Conserved role of spike S2 domain N-glycosylation across 
            betacoronaviruses.
  JOURNAL   Npj Viruses. 2025 Jan 25;3(1):4. doi: 10.1038/s44298-024-00085-7.
  PUBMED    40295734
REFERENCE   2  (bases 1 to 15390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Npj 
            Viruses."; date: "2025-01-25"; volume: "3(1):4. doi"; pages: " 
            10.1038/s44298-024-00085-7"
COMMENT     Sequence Label: LVDP 2P.2
FEATURES             Location/Qualifiers
     source          1..15390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2641..3229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2747..2758
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3156..3176
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     misc_feature    join(3500..3508,3509..3511,3512..4086)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3673..3692)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3902..3919
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4764..4817)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(6120..6323)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6324..6627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7559..7607
                     /note="synthetic polyadenylation signal"
     CDS             complement(8773..10425)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      10422..10431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10441..10652)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             11192..11372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11398..11415)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11404..11628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11674..12102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11761..11780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11971..11992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12111..12131)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12116..12445
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12296..12431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12358..12377
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12493..12540
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12559..12933
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13063..13184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13100..13119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13154..13173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13233..13249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13233..13249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13246..13268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13257..13273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13281..13287,13288..13305,13306..13311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13326..13347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13464..13481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13635..14223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13715..13734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14394..15185,15186..15254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15017..15036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15255..15359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcatc tttaatttct
     2401 aatatattta gaatctttaa tttctaatat atttagaatc tttaatttct aatatattta
     2461 gaatctttaa tttctaatat atttagaatc tttaatttct aatatattta gaatctttaa
     2521 tttctaatat atttagaatc tttaatttct aatatattta gaaggatctc cgcgtttatg
     2581 aacaaacgac ccaacacccg tgggtttttt ctgtcttttt attgccgtca ttctcgaggg
     2641 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agatgcggaa
     2701 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     2761 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccatg
     2821 gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     2881 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     2941 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     3001 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     3061 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     3121 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     3181 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgagtcggt
     3241 cgagttaaag aacgaggaga tccggcaccc tgctagaatt gagattcttc accctgctgt
     3301 aaacgtagaa tgagggtttc acgagagata cattgacaat gttacaacag taggcgcaaa
     3361 gcctcaatgc agtcagaatt gccagtgtga cgaggagaaa tacgacgaat gccagaaaca
     3421 agagcacaga atttacgatc agtgtcccgg tttcttctga tacgaagctg tacatggtgg
     3481 cggcgaattt ctccttgtgg gttgtggcaa gcttatcatc gtgtttttca aaggaaaacc
     3541 acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac acatgtaaag
     3601 catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc
     3661 agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc
     3721 acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg
     3781 ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac
     3841 gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt caacagacct
     3901 tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca
     3961 ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa catatagaca
     4021 aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg gggggaggga
     4081 gaggggcggg atccttactg gacgagcaaa gcaatgttat cagaagatga tgagtggtct
     4141 gtattcagtt tatagttccc tatcctgtag cgtgaatagg ctgcaaatcc actatcgcca
     4201 gcgacccttt gggacgctcc aagcttgtaa tagctcaaag ttcgactggt agctacagtt
     4261 atttcttttg gcaaatcctt aatatcacag cgaccaagat gatgtcctgc gatcctcaag
     4321 tggcccctca ggatcacggc tccaatcacc aattctgatt caaggagcgg tcgggtcaaa
     4381 attgtgccat gcagggggac attaagcaag atatttgttt cagggttaaa tgaccacatg
     4441 cttcgcgtgc gtgcaaaaag tctgaatgac gctataaaat aggagagcca catcaaacct
     4501 accaggcatg ccatagctat tgctatgccg ccagttatcc agtttattcg atacaccgcc
     4561 gcgagtacga agcaggcaag ggtcacaggc cacaggagcc aaaggaatat aagttttata
     4621 atatagagga atcggttgcg attcgcgtat gcgaattgca ggagacaaat ccatgtcaag
     4681 aagagaaatc caatcacaag gttccattgc tccaacagtt ttttcagctc ctctacagta
     4741 atggtcccat ttgagtcggc cattgggcca ggattctcct ccacgtcacc gcatgttagg
     4801 agacttcctc tgccctctcc tccggacccc tgggtgctat cggcgctaga catagactgc
     4861 tggagctgct tggaaaaatc gtccaggtcg gcggctggca gcagtgtcac ggtctgctgc
     4921 ttcttctgtc tctgaggcag ggcctgggtc tcatcggcct tcttcttctt gtccttcttg
     4981 ggctctgtag ggggaaaggt cttataggca tcgatgtgct tgttcagcag gatgacctga
     5041 tccttgaagt tagggtcctt atcgtccagc ttgatggcgc ctgtgtaggt cagccatgtg
     5101 ccggatgggg tcacctccat tccgatcctg ctcatgccaa agaaggcgga ggcgcttggt
     5161 gcgaactgtg cgatctgagg ccagtgctta taatcggtgc cctgcctgat cagctcctgg
     5221 tcgccaaaat tgccctgtgt ctgctctggt ccgcgcctgc cgaaggcctg ggtcacgttg
     5281 taggcctttg tggcggtgcg cttctgcctt ggcttcttgc tggcctcggc ggcagacttc
     5341 tttgtcacgg tctgtccctg ctgctgctgt cccttgccgc tcatcttaga ctccagctga
     5401 ttcagtctat ccagcagcag cagggccagg gcggcgtcgc ctccgtttcc tgccattctt
     5461 gcagggcttg tgcccatgga gctgcctggg gtagaattcc gagaggagtt tctggaccgg
     5521 ctagaggagc ggctagaggc ctgggagccg cccctgctgc cctctgcgta aaagcccttg
     5581 ggcagtgtgg tgccctgtgg cagctggagc acgattgctg cattgttggc gggattgcgt
     5641 gtgccgatgt gatcctttgg tgtgttcagg gcgccctcgg ttgccaccca gatgattccg
     5701 tccttattgg cgccatatgg cagtcctgcc tcaggtccgg tgcccagata gtagaagtac
     5761 catctggggg acagatcctt catcttgccg tcgcctcccc ggattctcct tgttgccctc
     5821 ctatagtagc cgatctgatc gtcaggggag ctattggtgt tgataggcac gccctggccc
     5881 ctgggaaact tcaggtcctc cttgccgtgc tgtgtcaggg cggtgaacca gctggcggta
     5941 ttgttgggca ggccctgtgg tctccgctgc ttagagcgtg ctccggacct ctctccattc
     6001 tggttggagc ctgtagaatc ggaggggccg ccgaaggtga ttcttggggc attccgctgg
     6061 ttctgagggc cgttgtcaga catggtggcg agctagcgga tctgacggtt cactaaacca
     6121 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     6181 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     6241 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     6301 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     6361 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     6421 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     6481 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     6541 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     6601 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     6661 cccgtaattg attactatta atcgaatttg tggatccgtg acgcactgaa caggttgccc
     6721 aaggaggctg tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg
     6781 ggcagcctgg gctgctggtt ggcgaccctg cacatagcag ggggttggag ctgggtgagc
     6841 actgtgctcc tttgcaaccc aggccattct atgattctgt cattctaaat ctctctttca
     6901 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     6961 cgttcagagg aaagcgatcg cgtgccacct tccccgtgcc gggctgtccc cgcacgctgc
     7021 cggctccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     7081 ctgctgcccc ctagcggaag agggatgcaa ttacatccct gggggctttg gcggggggct
     7141 ctcccttaat taactgacag ccacgatccc ataggtgaag gcaaaggcgt ggcagctagg
     7201 aacactcact acacccccac tcaagggaac accgtgccca gctaccctga gacagcccca
     7261 ctcccaggaa atgcaggtgc catgcctcac tggggctggt tggcacctga gccagacacc
     7321 cccaaatagt cccttcccac ctcctcaagc atctgcactc tgggtgaacc cagaaaaact
     7381 ggggtacctg gggagacata gaagggccag gccaggaact ccccaataag caggaacaga
     7441 gacctgaccc ctgagcctcc cccaccctct aaggctgctc aatgtcaagg caggagggat
     7501 ctccacacac ctgcactctg ggtaaggaca agttggcccc cactgcccca cctctagaca
     7561 cacaaaaaac caacacacag atctaatgaa aataaagatc ttttatttct agcaggcgat
     7621 cgcggagttg ggatctatct ttatagccac ggaacctcca agagctagct tttgttgtat
     7681 aaacccacaa atgtaagtga aaaaaccctc tttagagtca ttttcttttg taacattttt
     7741 agtcttaggg tcgtacatat cactaataat gagatcccat ttattagctg tatgtacagt
     7801 tgcacaatca ccaatcaaag ttgaatctgc atcagagaca aagtcattaa gatctgaatc
     7861 gacaagcagc gtacccgtag gcaaccactg tcttaaaaca gctgtacctg gtgcaactcc
     7921 tttatcagaa ccagcaccaa aatgtataac tctcatatta tagggtacag ctaatgttaa
     7981 tgtgtttaaa tattgacaca gttgagtata ttttgcgaca ttcatcatta tgcctttagg
     8041 taatgttgca ctatcaccat aattttgaag gtcacacttt tctaatagca ttctttgcat
     8101 tttgtaaaga ttaggcatag caacacccgg ttgccacgct tgactagatt gtaattttgg
     8161 gtaaaatgtt tctacatggc catctttaca ccaaagcata aatgaaattt ctgtatagtc
     8221 aatagtcact ttgacaacct tagaaactac agataaatct tgggatttta ttatttcaac
     8281 aaaatcatca agtaataaat caataacaga acacacacac ttagatgaac ctgtttgcgc
     8341 atctgttatg aaatagtttt taactgtact gtccatagga ataaaatctt ctaattcaaa
     8401 aggtgattcc ttaaaacgtt tagctagtcc aatcagtaga tgtaaaccac ctaactgact
     8461 atgactaaaa tctccataaa cgatatgttc gaaggcatag ccttctaatt tataccgttc
     8521 aatgaattca tccatagcta attctaagaa atcaatttcc atttgactcc tgggtttaaa
     8581 ttcttgtaaa tttctactct gagtaaagta agtttcaggt aattgttgga caacaccatc
     8641 aactttctta taataattga actgtgtttt tacggcttct ccaattaatg tgactccatt
     8701 aagactagct tgtttgggac ctacagagat ctatatctcc ttcttaaagg aggcggccgc
     8761 ataagcgaat tcttacacgg cgatcttgcc gcccttcttg gccttaatga gaatctcgcg
     8821 gatcttgcgg gcgtccaact tgccggtcag tcctttaggc acctcgtcca cgaacacaac
     8881 accaccgcgc agcttcttgg cggttgtaac ctggctggcc acatagtcca cgatctcctt
     8941 ctcggtcatg gttttaccgt gttccagcac gacgactgcg gcgggcagct cgccggcatc
     9001 gtcgtcgggc aggccggcga ccccggcgtc gaagatgttg gggtgttgca gcaggatgct
     9061 ctccagttcg gctggggcta cctggtagcc cttgtatttg atcaggctct tcagccggtc
     9121 cacgatgaag aagtgctcgt cctcgtccca gtaggcgatg tcgccgctgt gcagccagcc
     9181 gtccttgtcg atgagagcgt ttgtagcctc ggggttgtta acgtagccgc tcatgatcat
     9241 ggggccacgg acgcacagct cgccgcgctg gttcacaccc agtgtcttac cggtgtccaa
     9301 gtccaccacc ttagcctcga agaagggcac caccttgcct actgcgccag gcttgtcgtc
     9361 cccttcgggg gtgatcagaa tggcgctggt tgtttctgtc aggccgtagc cctggcggat
     9421 gcctggtagg tggaagcgtt tggccacggc ctcacctacc tccttgctga gcggcgcccc
     9481 gccgctggcg atctcgtgca agttgcttag gtcgtacttg tcgatgagag tgctcttagc
     9541 gaagaagcta aatagtgtgg gcaccagcag ggcagattga atcttatagt cttgcaagct
     9601 gcgcaagaat agctcctcct cgaagcggta catgagcacg acccgaaagc cgcagatcaa
     9661 gtagcccagc gtggtgaaca tgccgaagcc gtggtgaaat ggcaccacgc tgaggatagc
     9721 ggtgtcgggg atgatctggt tgccgaagat ggggtcgcgg gcatgactga atcggacaca
     9781 agcggtgcgg tgcggtaggg ctacgccctt gggcaatccg gtactgccac tactgttcat
     9841 gatcagggcg atggttttgt cccggtcgaa gctctcgggc acgaagtcgt actcgttgaa
     9901 gccgggtggc aaatgggaag tcacgaaggt gtacatgctt tggaagccct ggtagtcggt
     9961 cttgctatcc atgatgatga tcttttgtat gatcggtagc ttcttttgca cgttgaggat
    10021 cttttgcagc cctttcttgc tcacgaatac gacggtgggc tggctgatgc ccatgctgtt
    10081 cagcagctcg cgctcgttgt agatgtcgtt agctggggcc acagccacac cgatgaacag
    10141 ggcacccaac acgggcatga agaactgcaa gctattctcg ctgcacacca cgatccgatg
    10201 gtttgtattc agcccatagc gcttcatagc ttctgccagc cgaacgctca tctcgaagta
    10261 ctcggcgtag gtaatgtcca cctcgatatg tgcgtcggta aaggcgatgg tgccgggcac
    10321 cagggcgtag cgcttcatgg ctttgtgcag ctgctcgccg gcggtcccgt cttcgagtgg
    10381 gtagaatggc gctgggccct tcttaatgtt tttggcatct tccatggcgg cgctaccggt
    10441 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
    10501 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
    10561 gtttaccccg cgccaccttc tctaggcacc ggatcaattg ccgacccctc cccccaactt
    10621 ctcggggact gtgggcgatg tgcgctctgc ccacgtggaa ttatcgattc cgcggtgaat
    10681 tcttcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
    10741 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
    10801 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    10861 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
    10921 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
    10981 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
    11041 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
    11101 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
    11161 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
    11221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11341 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
    11401 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    11461 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    11521 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    11581 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
    11641 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
    11701 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    11761 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    11821 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    11881 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    11941 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    12001 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    12061 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
    12121 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    12181 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    12241 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    12301 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    12361 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
    12421 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
    12481 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
    12541 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
    12601 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
    12661 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
    12721 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
    12781 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
    12841 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
    12901 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
    12961 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    13021 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
    13081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
    13201 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    13261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    13321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    13381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    13441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    13501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    13561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    13621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    13681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    13741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    13801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    13861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    13921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    13981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    14041 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    14101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    14161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    14221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    14281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    14341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    14401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    14461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    14521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    14581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    14641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    14701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    14761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    14821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    14881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    14941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    15001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    15061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    15121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    15181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    15241 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    15301 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    15361 acatttcccc gaaaagtgcc acctgacgtc
//
