LOCUS       sequence-460019-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of recombinant His-tagged E1GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   De Lorenzi V, Ghignoli S, Bernardi M, Matteoli G, Ferri G, Storti B,
            Bizzarri R, Cardarelli F
  TITLE     Measuring pH in insulin secretory granules by phasor-based 
            fluorescence lifetime imaging of a genetically encoded sensor.
  JOURNAL   Commun Biol. 2025 Feb 25;8(1):304. doi: 10.1038/s42003-025-07758-w.
  PUBMED    40000885
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-02-25"; volume: "8(1):304. doi"; pages: " 
            10.1038/s42003-025-07758-w"
COMMENT     Sequence Label: pET-His-E1GFP
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(179..198)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             338..589
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     526..547
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
       61 caccctgagc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      121 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      181 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      241 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      301 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      361 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      421 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      481 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      541 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt agaatcttta
      601 ttttcaggga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc
      661 accaccacna ctgagannnc ggctgctaac aaagcccgna annnag
//
