LOCUS       sequence-460026-        9832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of destabilized CreER in zebrafish hepatocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Hovhannisyan GG, Akhourbi T, Eski SE, Pirson I, Gurzov EN, Singh SP
  TITLE     CellCousin2: An Optimized System for Partial Ablation and Tracing of
            Regenerative Lineages
  JOURNAL   bioRxiv 2025.05.23.655316
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.23.655316"
COMMENT     Sequence Label: fabp10a:DD-CreER; cryaa:mCerulean
FEATURES             Location/Qualifiers
     source          1..9832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(445..465)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(445..463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(496..513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(496..512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(653..1108)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(790..811)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1002..1021
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1135..1239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1240..1308,1309..2100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1458..1477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2271..2859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2760..2779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3013..3030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3147..3168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3183..3188,3189..3206,3207..3213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3221..3237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3226..3248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3245..3261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3245..3261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3307..3326
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3307..3325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5903..6379
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDHVIGMENAMPWSLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSADEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVGGDTHFPDYEPDDWGSVFSEFHDADAQNSHSYCFVILGRR"
     CDS             6386..7411
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             7424..8359
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             8369..8395
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8579..8700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8616..8635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8670..8689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    8743..8950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8939..8956
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(8974..9687,9688..9690,9691..9693))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9019..9040)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9368..9387
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagacccctg gttatatacc tgtgctgcag aaagaggggc ttacaaatgg atctctctgg
       61 aatggcatta tctcatggtg ggaacagtgt tgtcagcaga atcagcgggg tggactctga
      121 cctgagaatg acagtctagc actgaatggc tcagacaata ctcttgtggc caactatcac
      181 cggaaaataa gtgcctgcca aaacaaacca gaccagatga agctacatgg ctgtagaacg
      241 tcagtattta atgtgttgtg gccacttggt tcaccagatt taagagctaa tttttttaaa
      301 tatcacagat aatcaatcgc tttttttttt tcttgactga aaagcttaga ctggaaaatt
      361 ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg aatgcacact
      421 attaatggta cccagctttt gttcccttta gtgagggtta attgcgcgct agggataaca
      481 gggtaatacg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      601 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta
      661 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      721 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      781 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      841 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      901 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      961 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     1021 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     1081 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1141 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1201 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1261 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1321 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1381 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1441 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1501 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1561 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1621 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1681 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1741 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1801 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1861 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1921 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1981 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2041 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2101 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2161 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2401 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2941 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3001 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3061 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     3181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     3241 cacacaggaa acagctatga ccatgattac gccaagcgcg ttagggataa cagggtaatg
     3301 cgcgcgtaat acgactcact atagggcgaa ttggagctct gcagaagagc atctctgaac
     3361 acacaacacg tccaaccttg aggcagatgg gctacagcag cagaagacca caccgggtgc
     3421 cgctcctgtc agctaagaac aggaaactga ggctacaatt cacacagact caccaaaact
     3481 ggacgatata ttattagccc cccttagaat ttttcatttg ataatatttt tcttctggcg
     3541 aaagcctcat ttgttttata tattatagaa taaaattagt ttttaatagt ttttatgcca
     3601 ttttaaggtc aatattatta gcccctttaa gctatttttt ttcgatagtc tacagaacaa
     3661 accatcggta tacaatgact tgcctaatta ccctaacctg cctagttacc ctaattaacc
     3721 tagttaagcc tttaaatgtc actttaagct gtatagaagt gtcttgaaga atatctagtc
     3781 taatattatt gactgtcatc atggcaaaga taaaataaat cagttattaa aactattatg
     3841 attagaaatg tgctgaaaca atctgctctc cgataaacag aaattgaaca aaataaacag
     3901 gggggctaat aaatttaagg ggttaaataa ttctgattgc aaaaaaaatg atgtctgcaa
     3961 aactgttgca aataatttat ttgtgttgat tttaagcaaa caaattaaat ttaataaaat
     4021 acaacttaat ctgtttgttt aaattcagcc ctaataaatt gtttacagcc acttaacgta
     4081 aaaaaattga gtaaatccaa ggaatcatct ctgaataatt tttttcagtg tatatatata
     4141 tatatatatt cttacaaaac aactcattta ctttagttta attttcaggg gcaaaaaact
     4201 aaagtaatcg acgttgcttg aataaaaagt gtaattaagg gaatgaggta acatttaacc
     4261 atgtgtcaat gcagtttaaa tatgccagtt agtggtatat gtttaaatgg taagctattc
     4321 aaaactttaa actaacttaa ccagcctttt gttgtcagac tgaacagact ttccatctgc
     4381 attattagag actaatcttt ggctggatga atgattcatc tgctgatatt tcagaataga
     4441 cagattgagg ctgtttctaa tatgattatg caacctgagg gtgattattt gaagcaaact
     4501 ccacagacca gcaggtcatt gaccgtcgtg tgttcaaaca gagcagaaac atttgcaaaa
     4561 ctggtctgac aggagaatcc agtccagcac aacacatatg ctgagcaaac tgaatcaatc
     4621 ctgcaggtca actctcgtgc tttaagttta ttaagattat tttatttatt tattatttta
     4681 tttatctatt tatttattta gttgtttatt tagcaattat ttgtttattt atttgtttat
     4741 ttattcctgc agatcatgcc ttgtgccttt ttacatttaa tttaattttt aatttaattt
     4801 ccttttactt ttttttattt ttttatttta ttttatttta cagtctgaca aatactgaac
     4861 taaaaaccct cagatcatgt cttatgcatt tcattttatt ttatttcatt ttatattatt
     4921 aattttaata tttttatttt acagtctgac aaatactgaa ttaaaaacca tcagatcatg
     4981 tctcatgcat ttaacttaac tttatttaat tcaattaaat tgtttgtttg tttgtttcct
     5041 tgcatttgtt tgtttgtttt ttacaatctg acatactgga ccgaaaaaac tcagatcatg
     5101 tcttatgcat tttattttat tttatttttt attttattgt ctattttatt ttatttcatc
     5161 ttattttatt ttacagtctg acatatggac caataaatct ctcagatcat gtctcatgca
     5221 tttaatttaa tttttttatt tattcatttt tatttattta ttttattatt tattttattt
     5281 taatatttat tttattttat tttattttat tttacagtct gacatactgg accaataaat
     5341 ctctcagatc atgtctcatg cattttactt ttattttatt agaattagaa agatcaaagg
     5401 aacaactttt aaaatattaa ttctgtatca aaatctcttt tgatacattt aattgattta
     5461 aaaaagcagt tcacccaaga aacatttcct cacagtcgaa tggttgtaaa cttttatgaa
     5521 ttactttcac agaaaaagat ttttggaaga atattggaaa aaaagcagcc attgacttcc
     5581 atagtaacaa caaaaaatac tatggaagtc aatggctgtt ttttcaccat tcggtatctt
     5641 cattctggag cagaattttt tgggtgatct gtccctttaa gtcgtcaaat cctggtgcaa
     5701 tattccacat gcactgacga gtctttattt ttggtctgct actgctgtgt gtgtgagggc
     5761 atttacctca tcttctgctg gagttgatga acggtgggtt gttcaaacag cagcaggtca
     5821 ttgactgaac tcctctcgat ataaaagctg cagatctgaa gctgaccttc actttgtgtt
     5881 gatcgctagc gaattcgcca ccatgatcag tctgattgcg gcgttagcgg tagatcacgt
     5941 tatcggcatg gaaaacgcca tgccgtggag cctgcctgcc gatctcgcct ggtttaaacg
     6001 caacacctta aataaacccg tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc
     6061 gttgccagga cgcaaaaaca ttatcctcag cagtcaaccg agtacggacg atcgcgtaac
     6121 gtgggtgaag tcggcggatg aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt
     6181 gattggcggc ggtcgcgttt atgagcagtt cttgccaaaa gcgcaaaaac tgtatctgac
     6241 gcatatcgac gcagaagtgg gaggcgacac ccatttcccg gattacgagc cggatgactg
     6301 gggatcggta ttcagcgagt tccacgatgc tgatgcgcag aactctcaca gctattgctt
     6361 tgtgattctg gggcggcgag catgctccaa tttactgacc gtacaccaaa atttgcctgc
     6421 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga
     6481 tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc
     6541 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga
     6601 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg
     6661 ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt
     6721 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca
     6781 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga
     6841 tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg
     6901 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat
     6961 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact
     7021 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga
     7081 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac
     7141 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta
     7201 cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt
     7261 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg
     7321 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg
     7381 ggcaatggtg cgcctgctgg aagatggcga tctcgagcca tctgctggag acatgagagc
     7441 tgccaacctt tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt
     7501 gtccctgacg gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta
     7561 ttccgagtat gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa
     7621 cctggcagac agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt
     7681 ggatttgacc ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat
     7741 gattggtctc gtctggcgct ccatggagca cccagtgaag ctactgtttg ctcctaactt
     7801 gctcttggac aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct
     7861 gctggctaca tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct
     7921 caaatctatt attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc
     7981 tctggaagag aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca
     8041 cctgatggcc aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct
     8101 cctcatcctc tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat
     8161 gaagtgcaag aacgtggtgc ccctctatga cctgctgctg gaggcggcgg acgcccaccg
     8221 cctacatgcg cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt
     8281 ggccactgcg ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc
     8341 agagggtttc cctgccacag ctgtcgacta cccatacgac gtaccagatt acgcttgatt
     8401 aattaagtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcaggcg
     8461 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     8521 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8581 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8641 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8701 atcgaggggg ggcccggtac ccaccgcggt ggcggcgatc tctccccagc atgcctgcta
     8761 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     8821 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     8881 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     8941 gaaggcacag tcgaggctga tcagcgggcc gctttacttg tacagctcgt ccatgccgag
     9001 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     9061 gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     9121 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     9181 gttgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata
     9241 gacgttgtcg ctgatggcgt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     9301 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     9361 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     9421 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
     9481 gcactgcacg ccccaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     9541 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     9601 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     9661 cccggtgaac agctcctcgc ccttgctcac catgttgtca gacctggtaa ctccttactg
     9721 taacttattg cacctgatga acaaaagatg tgaactgaac gcaagtagga ggctaataga
     9781 ggctagtaca gtacaaagat ccccaaatga tgtgtgccgg ccttagtgga gg
//
