LOCUS       sequence-460027-       11658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Partial ablation using NTR2 and subsequent lineage tracing of 
            regenerating hepatocytes in zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Hovhannisyan GG, Akhourbi T, Eski SE, Pirson I, Gurzov EN, Singh SP
  TITLE     CellCousin2: An Optimized System for Partial Ablation and Tracing of
            Regenerative Lineages
  JOURNAL   bioRxiv 2025.05.23.655316
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.23.655316"
COMMENT     Sequence Label: 
            fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCh
            erry-NTR2.0; cmlc2:EGFP
FEATURES             Location/Qualifiers
     source          1..11658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..52
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(204..215,216..403)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     CDS             join(1384..1386,1387..1389,1390..2103)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1384..1386,1387..1389,1390..2100)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1690..1709)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2037..2058
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2121..2138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2127..2334
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2362..2483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2826..2947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3777..4484)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4052..4071)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4321..4339
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4481..4490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4498..4531
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4538..4659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4780..5493,5494..5496,5497..5499))
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     regulatory      5496..5505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5584..5961)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     protein_bind    5981..6014
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    6097..6218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6346..6372)
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             complement(6373..7080)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             complement(7081..7128)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    7150..7183
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7192..7225
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    join(9852..10014,10015..10026)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        10066..10170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10171..10239,10240..11031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10389..10408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11202..11658,1..132)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
       61 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      181 tttgctcaca tgttctttcc gatcagaggt gtaaagtact tgagtaattt tacttgatta
      241 ctgtacttaa gtattatttt tggggatttt tactttactt gagtacaatt aaaaatcaat
      301 acttttactt ttacttaatt acattttttt agaaaaaaaa gtacttttta ctccttacaa
      361 ttttatttac agtcaaaaag tacttatttt ttggagatca cttcattcta ttttcccttg
      421 ctattaccaa accaattgaa ttgcgctgat gcccagttta attaccctgt tatccctagg
      481 taccaaagct taaatcagtt gtgttaaata agagacattc aaaataaatg taaatgacgt
      541 ctccaaatca gcagacttaa cattctttaa aatgattgat tcaatagtga taaaaatcag
      601 gcatagccag ttgtaacttt agataaatta cagaaaatgt caaatacaga gaaccgattc
      661 ttttttatga tacatccaag cacacattta acacaatcca ggcaaacccc gaatttcaca
      721 gtcacaagca ctgtttgtac aagagctttg cctaaggaca cacagtctct ataagtccag
      781 gtcgttggtt tcactcttat tttaaacatg tgacattttt cctgccatcc tgtcttaggc
      841 tgctgtttgc ttcattccat gtcacattaa attcctcagt agcacctttt acacacacag
      901 ccaatctttt ccagaaaatt caattgcttt gaagagataa tgtgtgaaca aatccattta
      961 gaaaaggaaa attaagaatt tgtaaaatca tctgtaaatt gttggcattc ttctgtatat
     1021 gaacatcaca tcatttacag gtaaaggtct ggtcattaat tatatgacaa tttactggta
     1081 ttattttgtg aaaggggcta ttttcaatgc gttcatccat ccttttcatc cctcaaatct
     1141 ctcattcacg tccccctccc catctgcaca ctttatctca ttttccaccc tgctggaatc
     1201 tgagcacttg tgcagttatc agggctcctg tatttaggag gctctgggtg tccatgtagg
     1261 ggacgaacag aaacactgca gacctttata gaagaacaat tgataagagt cctcatacat
     1321 aaagactcca ttagtaagcc agtgacccag gagcccagac caacagcaaa gcagacagtg
     1381 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1441 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1501 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1561 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1621 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1681 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1741 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1801 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1861 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1921 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1981 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2041 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2101 taaagcggcc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2161 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     2221 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtgggtt
     2281 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggagagatc
     2341 gccgccaccg cggtgggtac ctaagataca ttgatgagtt tggacaaacc acaactagaa
     2401 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2461 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2521 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     2581 attatgatct agagtcgcgg ccgcttaatt aagtcgacat gcatggaggg agaagtggcg
     2641 ggggtggggg cgagggtggg ggggatgatg gcatgcagca ctggggttcc ttaggaggga
     2701 caaggtcccc gtttctcccc atgttctgag atatttgaag gacttgggga gggagtcagc
     2761 tgaccgggag aagcaagacc tctgcagagg aaggacctcg aattaacgct tacaatttac
     2821 gcctataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2881 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2941 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     3001 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
     3061 cctaggccgc ttcagatttc ggtaaaaaca gtctctgctg accaacgtga ttttggcagc
     3121 ttagcattaa aatcgtcttc gctatggtaa cccagcggca caatcaccac gctggtaaag
     3181 cccttctcac gcagaccaaa ctcctcatca agcaccttgg catcgaagcc ttcaattggc
     3241 accgcgtcga tctccatcgc agaagcaccc aatagcagcg tccccacatt gaggtaaact
     3301 tgcttttcca tccagtgatg cgcgtctttc aagtcaaagc ggtgcatgtt gacgaaaaat
     3361 gaacgaccag cgtgcatgcc attttttgct tcgacatcgg cgtagcggcc atctttgtct
     3421 tcgctttcca aaaggtcgag taggtacgct tcatcaatcg ccgttttggc gcagaacacc
     3481 acaacgtgag aagcatccaa aatcttgcgc tcattagcag caaaaccacc ttgtgttgct
     3541 ttggcgatgc gcgcttttcc ctcttcactg ctcgcgacaa taaaatgcca aggttgcgag
     3601 ttgacactgg acgcactcat gcggattaac tctttcactg ccgcgacttt ctcttcaggc
     3661 aatttgcgcg aagcatcaaa ggctttggtg gagtagcggg attgggcagc ttgaacaata
     3721 gtcatggcgg cgccgccgcc tgagttcgat gcttgagcac gtgatctgag ggatcccttg
     3781 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
     3841 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
     3901 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
     3961 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
     4021 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
     4081 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
     4141 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
     4201 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
     4261 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
     4321 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
     4381 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
     4441 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc gaccggtata
     4501 acttcgtata atgtatgcta tacgaagtta tctcgattaa gatacattga tgagtttgga
     4561 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4621 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4681 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     4741 aaatgtggta tggctgatta tgatctagag tcggccgctt tacttgtaca gctcgtccat
     4801 gtcatgtgta atcccggcgg cggtcaccct ctccttcagg accatgtgat cgcgcttctc
     4861 gttggggtct ttgctcagtt tggactgatg gctcaggtag tggttgtcgg gcagcagcac
     4921 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc
     4981 ctcaacgttg tggcgggtct tgaagttagc cttgatgccg ttcttctgct tgtcggccgt
     5041 gatatagacc ttgtggctgt tgaagttgta ctccagcttg tgccccagga tgttgccgtc
     5101 ctccttgaag tcgatgccct tcagcacgat gcggttcacc agggtgtcgc cctcgaactt
     5161 cacctcggcg cgggtcttgt aggttccgtc gtccttgaaa gagatggtgc gctcctggac
     5221 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     5281 ggcgaagcag gccacgccgt agcctaaggt ggtcacgagg gtgggccagg gcacgggcag
     5341 cttgccggtg gtgcagatga acttcagggt cagcttgccg ttggtggcat cgccctcgcc
     5401 ctcgccgcgg acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     5461 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcccgcg gtagcgctag
     5521 cccacctccg ccgctgcccc cacctccaga tccacccccg ccggagcctc cacccccaga
     5581 tcccttagcg ctggtgtact tggtgacggc cttagtaccc tcggacacgg cgtgcttggc
     5641 caactcccca ggcagcagca ggcgcacggc cgtctggatc tccctggagg tgatggtcga
     5701 gcgcttgttg taatgcgcca ggcgggaagc ctcacctgcg atgcgctcga aaatgtcgtt
     5761 cacaaacgag ttcatgatgc ccatggcctt ggacgaaatg ccggtgtcag ggtggacctg
     5821 cttcagaacc ttgtacacat agatggaata gctctccttg cggctgcgct tgcgcttctt
     5881 gccgcctttc ttctgcgcct tagtcaccgc cttcttggag ccctttttcg gggcgggagc
     5941 agacttcgct ggctctggca tggtggcgac cggtggatca ataacttcgt ataaagtatc
     6001 ctatacgaag ttatcctaga tcctagagtc gcggccaatt cctacaggaa caggtggtgg
     6061 cggccctcgg agcattaacg cttacaattt acgccttaag atacattgat gagtttggac
     6121 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6181 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6241 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     6301 aatgtggtat ggctgattat gatctagagt cggccgcaca ctttaatcca gcttcttttt
     6361 cttcgctgct ggattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac
     6421 tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc tttccagtct
     6481 gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct tggatctata
     6541 tgtggtcttg gcgtttgcga tcagatggct cccgcccacg agcttcaggg ccatgtcgtt
     6601 tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct cccagccgag
     6661 tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt
     6721 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
     6781 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct tgaagaagtc
     6841 ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc tagtagccag
     6901 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt
     6961 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt tgtccacggt
     7021 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcttcgc ccttagacac
     7081 ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttca tggtggcaga
     7141 tctgccggta taacttcgta taatgtatgc tatacgaagt tatctcgagt tataacttcg
     7201 tataaagtat cctatacgaa gttatgagat cggcgcgccg aattcgctag cgatcaacac
     7261 aaagtgaagg tcagcttcag atctgcagct tttatatcga gaggagttca gtcaatgacc
     7321 tgctgctgtt tgaacaaccc accgttcatc aactccagca gaagatgagg taaatgccct
     7381 cacacacaca gcagtagcag accaaaaata aagactcgtc agtgcatgtg gaatattgca
     7441 ccaggatttg acgacttaaa gggacagatc acccaaaaaa ttctgctcca gaatgaagat
     7501 accgaatggt gaaaaaacag ccattgactt ccatagtatt ttttgttgtt actatggaag
     7561 tcaatggctg ctttttttcc aatattcttc caaaaatctt tttctgtgaa agtaattcat
     7621 aaaagtttac aaccattcga ctgtgaggaa atgtttcttg ggtgaactgc ttttttaaat
     7681 caattaaatg tatcaaaaga gattttgata cagaattaat attttaaaag ttgttccttt
     7741 gatctttcta attctaataa aataaaagta aaatgcatga gacatgatct gagagattta
     7801 ttggtccagt atgtcagact gtaaaataaa ataaaataaa ataaaataaa tattaaaata
     7861 aaataaataa taaaataaat aaataaaaat gaataaataa aaaaattaaa ttaaatgcat
     7921 gagacatgat ctgagagatt tattggtcca tatgtcagac tgtaaaataa aataagatga
     7981 aataaaataa aatagacaat aaaataaaaa ataaaataaa ataaaatgca taagacatga
     8041 tctgagtttt ttcggtccag tatgtcagat tgtaaaaaac aaacaaacaa atgcaaggaa
     8101 acaaacaaac aaacaattta attgaattaa ataaagttaa gttaaatgca tgagacatga
     8161 tctgatggtt tttaattcag tatttgtcag actgtaaaat aaaaatatta aaattaataa
     8221 tataaaatga aataaaataa aatgaaatgc ataagacatg atctgagggt ttttagttca
     8281 gtatttgtca gactgtaaaa taaaataaaa taaaaaaata aaaaaaagta aaaggaaatt
     8341 aaattaaaaa ttaaattaaa tgtaaaaagg cacaaggcat gatctgcagg aataaataaa
     8401 caaataaata aacaaataat tgctaaataa acaactaaat aaataaatag ataaataaaa
     8461 taataaataa ataaaataat cttaataaac ttaaagcacg agagttgacc tgcaggattg
     8521 attcagtttg ctcagcatat gtgttgtgct ggactggatt ctcctgtcag accagttttg
     8581 caaatgtttc tgctctgttt gaacacacga cggtcaatga cctgctggtc tgtggagttt
     8641 gcttcaaata atcaccctca ggttgcataa tcatattaga aacagcctca atctgtctat
     8701 tctgaaatat cagcagatga atcattcatc cagccaaaga ttagtctcta ataatgcaga
     8761 tggaaagtct gttcagtctg acaacaaaag gctggttaag ttagtttaaa gttttgaata
     8821 gcttaccatt taaacatata ccactaactg gcatatttaa actgcattga cacatggtta
     8881 aatgttacct cattccctta attacacttt ttattcaagc aacgtcgatt actttagttt
     8941 tttgcccctg aaaattaaac taaagtaaat gagttgtttt gtaagaatat atatatatat
     9001 atatacactg aaaaaaatta ttcagagatg attccttgga tttactcaat ttttttacgt
     9061 taagtggctg taaacaattt attagggctg aatttaaaca aacagattaa gttgtatttt
     9121 attaaattta atttgtttgc ttaaaatcaa cacaaataaa ttatttgcaa cagttttgca
     9181 gacatcattt tttttgcaat cagaattatt taacccctta aatttattag cccccctgtt
     9241 tattttgttc aatttctgtt tatcggagag cagattgttt cagcacattt ctaatcataa
     9301 tagttttaat aactgattta ttttatcttt gccatgatga cagtcaataa tattagacta
     9361 gatattcttc aagacacttc tatacagctt aaagtgacat ttaaaggctt aactaggtta
     9421 attagggtaa ctaggcaggt tagggtaatt aggcaagtca ttgtataccg atggtttgtt
     9481 ctgtagacta tcgaaaaaaa atagcttaaa ggggctaata atattgacct taaaatggca
     9541 taaaaactat taaaaactaa ttttattcta taatatataa aacaaatgag gctttcgcca
     9601 gaagaaaaat attatcaaat gaaaaattct aaggggggct aataatatat cgtccagttt
     9661 tggtgagtct gtgtgaattg tagcctcagt ttcctgttct tagctgacag gagcggcacc
     9721 cggtgtggtc ttctgctgct gtagcccatc tgcctcaagg ttggacgtgt tgtgtgttca
     9781 gagatgctct tctgcagagc tcgagattac cctgttatcc ctaaagctta aacaagaatc
     9841 tctagttttc tttcttgctt ttacttttac ttccttaata ctcaagtaca attttaatgg
     9901 agtacttttt tacttttact caagtaagat tctagccaga tacttttact tttaattgag
     9961 taaaattttc cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca
    10021 cctctgatca ttgggacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    10081 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    10141 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    10201 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    10261 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    10321 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    10381 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
    10441 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
    10501 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
    10561 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10621 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10681 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    10741 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    10801 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10861 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10921 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10981 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    11041 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    11101 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    11161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    11221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11341 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11581 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11641 ccggtaagcg gcagggtc
//
