LOCUS       sequence-460028-       10215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ERCreER in zebrafish hepatocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Hovhannisyan GG, Akhourbi T, Eski SE, Pirson I, Gurzov EN, Singh SP
  TITLE     CellCousin2: An Optimized System for Partial Ablation and Tracing of
            Regenerative Lineages
  JOURNAL   bioRxiv 2025.05.23.655316
REFERENCE   2  (bases 1 to 10215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.23.655316"
COMMENT     Sequence Label: fabp10a:ER-CreER; cryaa:mCerulean
FEATURES             Location/Qualifiers
     source          1..10215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(359..361,362..364,365..1078)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(665..684)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1012..1033
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1096..1113)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1102..1309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1352..1473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1363..1382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1417..1436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1597..2490)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     CDS             complement(1603..2538)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(2551..3573)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             complement(3583..4518)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     regulatory      4518..4527
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7109..7128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7110..7128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7174..7190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7174..7190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7187..7209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7198..7214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7222..7228,7229..7246,7247..7252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7267..7288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7405..7422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7576..8164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7656..7675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8335..9126,9127..9195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8958..8977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9196..9300)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9327..9782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9414..9433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9624..9645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9908..9930
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9922..9939
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9923..9939
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9970..9990
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9972..9990
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 catctggtct ggtttgtttt ggcaggcact tattttccgg tgatagttgg ccacaagagt
       61 attgtctgag ccattcagtg ctagactgtc attctcaggt cagagtccac cccgctgatt
      121 ctgctgacaa cactgttccc accatgagat aatgccattc cagagagatc catttgtaag
      181 cccctctttc tgcagcacag gtatataacc aggggtctgc ctccactaag gccggcacac
      241 atcatttggg gatctttgta ctgtactagc ctctattagc ctcctacttg cgttcagttc
      301 acatcttttg ttcatcaggt gcaataagtt acagtaagga gttaccaggt ctgacaacat
      361 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      421 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      481 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      541 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
      601 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      661 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      721 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      781 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
      841 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      901 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      961 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1021 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     1081 cggcccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     1141 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1201 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     1261 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggag agatcgccgc
     1321 caccgcggtg ggtaccgggc cccccctcga ttaagataca ttgatgagtt tggacaaacc
     1381 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     1441 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1501 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     1561 ggtatggctg attatgatct agagtcgcgg ccgctatcaa gctgtggcag ggaaaccctc
     1621 tgcctccccc gtgatgtaat acttttgcaa ggaatgcgat gaagtagagc ccgcagtggc
     1681 caagtggctt tggtccgtct cctccacgga tgcccctcca cggctagtgg gcgcatgtag
     1741 gcggtgggcg tccgccgcct ccagcagcag gtcatagagg ggcaccacgt tcttgcactt
     1801 catgctgtac agatgctcca tgcctttgtt actcatgtgc ctgatgtggg agaggatgag
     1861 gaggagctgg gccagccgct ggtgctgctg ctgcagggtc aggcctgcct tggccatcag
     1921 gtggatcaaa gtgtctgtga tcttgtccag gactcggtgg atatggtcct tctcttccag
     1981 agacttcagg gtgctggaca gaaatgtgta cactccagaa ttaagcaaaa taatagattt
     2041 gaggcacaca aactcctctc cctgcagatt catcatgcgg aaccgagatg atgtagccag
     2101 cagcatgtcg aagatctcca ccatgccctc tacacatttt ccctggttcc tgtccaagag
     2161 caagttagga gcaaacagta gcttcactgg gtgctccatg gagcgccaga cgagaccaat
     2221 catcaggatc tctagccagg cacattctag aaggtggacc tgatcatgga gggtcaaatc
     2281 cacaaagcct ggcaccctct tcgcccagtt gatcatgtga accagctccc tgtctgccag
     2341 gttggtcagt aagcccatca tcgaagcttc actgaagggt ctggtaggat catactcgga
     2401 atagagtatg gggggctcag catccaacaa ggcactgacc atctggtcgg ccgtcaggga
     2461 caaggccagg ctgttcttct tagagcgttt gatcatgagc gggcttggcc aaaggttggc
     2521 agctctcatg tctccagcag atggctcgag atcgccatct tccagcaggc gcaccattgc
     2581 ccctgtttca ctatccaggt tacggatata gttcatgaca atatttacat tggtccagcc
     2641 accagcttgc atgatctccg gtattgaaac tccagcgcgg gccatatctc gcgcggctcc
     2701 gacacgggca ctgtgtccag accaggccag gtatctctga ccagagtcat ccttagcgcc
     2761 gtaaatcaat cgatgagttg cttcaaaaat cccttccagg gcgcgagttg atagctggct
     2821 ggtggcagat ggcgcggcaa caccattttt tctgacccgg caaaacaggt agttattcgg
     2881 atcatcagct acaccagaga cggaaatcca tcgctcgacc agtttagtta cccccaggct
     2941 aagtgccttc tctacacctg cggtgctaac cagcgttttc gttctgccaa tatggattaa
     3001 cattctccca ccgtcagtac gtgagatatc tttaaccctg atcctggcaa tttcggctat
     3061 acgtaacagg gtgttataag caatccccag aaatgccaga ttacgtatat cctggcagcg
     3121 atcgctattt tccatgagtg aacgaacctg gtcgaaatca gtgcgttcga acgctagagc
     3181 ctgttttgca cgttcaccgg catcaacgtt ttcttttcgg atccgccgca taaccagtga
     3241 aacagcattg ctgtcacttg gtcgtggcag cccggaccga cgatgaagca tgtttagctg
     3301 gcccaaatgt tgctggatag tttttactgc cagaccgcgc gcctgaagat atagaagata
     3361 atcgcgaaca tcttcaggtt ctgcgggaaa ccatttccgg ttattcaact tgcaccatgc
     3421 cgcccacgac cggcaaacgg acagaagcat tttccaggta tgctcagaaa acgcctggcg
     3481 atccctgaac atgtccatca ggttcttgcg aacctcatca ctcgttgcat cgaccggtaa
     3541 tgcaggcaaa ttttggtgta cggtcagtaa attgtcgaca gctgtggcag ggaaaccctc
     3601 tgcctccccc gtgatgtaat acttttgcaa ggaatgcgat gaagtagagc ccgcagtggc
     3661 caagtggctt tggtccgtct cctccacgga tgcccctcca cggctagtgg gcgcatgtag
     3721 gcggtgggcg tccgccgcct ccagcagcag gtcatagagg ggcaccacgt tcttgcactt
     3781 catgctgtac agatgctcca tgcctttgtt actcatgtgc ctgatgtggg agaggatgag
     3841 gaggagctgg gccagccgct ggtgctgctg ctgcagggtc aggcctgcct tggccatcag
     3901 gtggatcaaa gtgtctgtga tcttgtccag gactcggtgg atatggtcct tctcttccag
     3961 agacttcagg gtgctggaca gaaatgtgta cactccagaa ttaagcaaaa taatagattt
     4021 gaggcacaca aactcctctc cctgcagatt catcatgcgg aaccgagatg atgtagccag
     4081 cagcatgtcg aagatctcca ccatgccctc tacacatttt ccctggttcc tgtccaagag
     4141 caagttagga gcaaacagta gcttcactgg gtgctccatg gagcgccaga cgagaccaat
     4201 catcaggatc tctagccagg cacattctag aaggtggacc tgatcatgga gggtcaaatc
     4261 cacaaagcct ggcaccctct tcgcccagtt gatcatgtga accagctccc tgtctgccag
     4321 gttggtcagt aagcccatca tcgaagcttc actgaagggt ctggtaggat catactcgga
     4381 atagagtatg gggggctcag catccaacaa ggcactgacc atctggtcgg ccgtcaggga
     4441 caaggccagg ctgttcttct tagagcgttt gatcatgagc gggcttggcc aaaggttggc
     4501 agctctcatg tctccagcca tggtggcggc tcacccggga attcgctagc gatcaacaca
     4561 aagtgaaggt cagcttcaga tctgcagctt ttatatcgag aggagttcag tcaatgacct
     4621 gctgctgttt gaacaaccca ccgttcatca actccagcag aagatgaggt aaatgccctc
     4681 acacacacag cagtagcaga ccaaaaataa agactcgtca gtgcatgtgg aatattgcac
     4741 caggatttga cgacttaaag ggacagatca cccaaaaaat tctgctccag aatgaagata
     4801 ccgaatggtg aaaaaacagc cattgacttc catagtattt tttgttgtta ctatggaagt
     4861 caatggctgc tttttttcca atattcttcc aaaaatcttt ttctgtgaaa gtaattcata
     4921 aaagtttaca accattcgac tgtgaggaaa tgtttcttgg gtgaactgct tttttaaatc
     4981 aattaaatgt atcaaaagag attttgatac agaattaata ttttaaaagt tgttcctttg
     5041 atctttctaa ttctaataaa ataaaagtaa aatgcatgag acatgatctg agagatttat
     5101 tggtccagta tgtcagactg taaaataaaa taaaataaaa taaaataaat attaaaataa
     5161 aataaataat aaaataaata aataaaaatg aataaataaa aaaattaaat taaatgcatg
     5221 agacatgatc tgagagattt attggtccat atgtcagact gtaaaataaa ataagatgaa
     5281 ataaaataaa atagacaata aaataaaaaa taaaataaaa taaaatgcat aagacatgat
     5341 ctgagttttt tcggtccagt atgtcagatt gtaaaaaaca aacaaacaaa tgcaaggaaa
     5401 caaacaaaca aacaatttaa ttgaattaaa taaagttaag ttaaatgcat gagacatgat
     5461 ctgatggttt ttaattcagt atttgtcaga ctgtaaaata aaaatattaa aattaataat
     5521 ataaaatgaa ataaaataaa atgaaatgca taagacatga tctgagggtt tttagttcag
     5581 tatttgtcag actgtaaaat aaaataaaat aaaaaaataa aaaaaagtaa aaggaaatta
     5641 aattaaaaat taaattaaat gtaaaaaggc acaaggcatg atctgcagga ataaataaac
     5701 aaataaataa acaaataatt gctaaataaa caactaaata aataaataga taaataaaat
     5761 aataaataaa taaaataatc ttaataaact taaagcacga gagttgacct gcaggattga
     5821 ttcagtttgc tcagcatatg tgttgtgctg gactggattc tcctgtcaga ccagttttgc
     5881 aaatgtttct gctctgtttg aacacacgac ggtcaatgac ctgctggtct gtggagtttg
     5941 cttcaaataa tcaccctcag gttgcataat catattagaa acagcctcaa tctgtctatt
     6001 ctgaaatatc agcagatgaa tcattcatcc agccaaagat tagtctctaa taatgcagat
     6061 ggaaagtctg ttcagtctga caacaaaagg ctggttaagt tagtttaaag ttttgaatag
     6121 cttaccattt aaacatatac cactaactgg catatttaaa ctgcattgac acatggttaa
     6181 atgttacctc attcccttaa ttacactttt tattcaagca acgtcgatta ctttagtttt
     6241 ttgcccctga aaattaaact aaagtaaatg agttgttttg taagaatata tatatatata
     6301 tatacactga aaaaaattat tcagagatga ttccttggat ttactcaatt tttttacgtt
     6361 aagtggctgt aaacaattta ttagggctga atttaaacaa acagattaag ttgtatttta
     6421 ttaaatttaa tttgtttgct taaaatcaac acaaataaat tatttgcaac agttttgcag
     6481 acatcatttt ttttgcaatc agaattattt aaccccttaa atttattagc ccccctgttt
     6541 attttgttca atttctgttt atcggagagc agattgtttc agcacatttc taatcataat
     6601 agttttaata actgatttat tttatctttg ccatgatgac agtcaataat attagactag
     6661 atattcttca agacacttct atacagctta aagtgacatt taaaggctta actaggttaa
     6721 ttagggtaac taggcaggtt agggtaatta ggcaagtcat tgtataccga tggtttgttc
     6781 tgtagactat cgaaaaaaaa tagcttaaag gggctaataa tattgacctt aaaatggcat
     6841 aaaaactatt aaaaactaat tttattctat aatatataaa acaaatgagg ctttcgccag
     6901 aagaaaaata ttatcaaatg aaaaattcta aggggggcta ataatatatc gtccagtttt
     6961 ggtgagtctg tgtgaattgt agcctcagtt tcctgttctt agctgacagg agcggcaccc
     7021 ggtgtggtct tctgctgctg tagcccatct gcctcaaggt tggacgtgtt gtgtgttcag
     7081 agatgctctt ctgcagagct ccaattcgcc ctatagtgag tcgtattacg cgcgcattac
     7141 cctgttatcc ctaacgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7201 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7261 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7321 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7381 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9301 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     9361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9481 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9541 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9661 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9721 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9781 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     9841 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     9901 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgtatt accctgttat
     9961 ccctagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccat taatagtgtg
    10021 cattcagtgc aggatgttat gtatttattt tctgtagtgc aaactttttc aaagaatttt
    10081 ccagtctaag cttttcagtc aagaaaaaaa aaaagcgatt gattatctgt gatatttaaa
    10141 aaaattagct cttaaatctg gtgaaccaag tggccacaac acattaaata ctgacgttct
    10201 acagccatgt agctt
//
