LOCUS       sequence-460047-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid was designed to selectively monitor the activation of 
            the STAT1 signaling pathway in NK cells, a pathway known to play a 
            crucial role in regulating NK cells' activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Min JY, Kim HM, Lee H, Cho MY, Park HS, Lee SY, Park MS, Ha SK, Kim 
            D, Jeong HG, Kim TD, Hong KS, Han EH
  TITLE     STAT1 as a tool for non-invasive monitoring of NK cell activation in
            cancer.
  JOURNAL   Commun Biol. 2024 Sep 30;7(1):1222. doi: 10.1038/s42003-024-06917-9.
  PUBMED    39349746
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-09-30"; volume: "7(1):1222. doi"; pages: " 
            10.1038/s42003-024-06917-9"
COMMENT     Sequence Label: pGF-GAS4X
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(136..180)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    365..598
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1091..1216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1263..1443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1444..1670)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1685..1702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1685..1701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1694..1716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1910..1929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2029..2051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2089..2107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2175..2279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2280..2348,2349..3140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2498..2517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3311..3899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4053..4070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4187..4208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4223..4228,4229..4246,4247..4253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4261..4277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4266..4288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4285..4301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4285..4301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      4334..4469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4509..4630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4520..4539)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4574..4593
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4702..4935
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5009..5597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5115..5126
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5524..5544
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5604..6203)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5687..5707)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6184..6203
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6216..6715)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6264..6283)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6657..6680
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(join(6833..7546,7547..7549,7550..7552))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6836..7546,7547..7549,7550..7552))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6878..6899)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7227..7246
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7486..7507
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(7559..7612)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7619..9262)
                     /codon_start=1
                     /product="intracellular red firefly luciferase"
                     /label=Red Firefly Luciferase
                     /note="human codon-optimized"
                     /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
                     NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
                     APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
                     QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
                     HARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
                     YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
                     GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
                     MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
                     VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
                     VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
     regulatory      9259..9268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9350..9374)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    9516..9632
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
       61 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
      121 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
      181 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
      241 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
      301 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
      361 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
      421 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
      481 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
      541 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
      601 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
      661 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
      721 atctcctcct ccaggtctga agatcagtgg ccgcttgctg tgcggtggtc ttacttttgt
      781 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
      841 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
      901 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
      961 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
     1021 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
     1081 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
     1141 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
     1201 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
     1261 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     1321 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     1381 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     1441 cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt
     1501 ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca
     1561 ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta
     1621 actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca cgcgtcagct
     1681 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1741 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1801 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     1861 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     1921 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     1981 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2041 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2101 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2521 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3961 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4021 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4081 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4141 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4201 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4261 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttggtct
     4321 ctgcaaaagt ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     4381 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     4441 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc
     4501 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4561 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4621 taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     4681 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     4741 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     4801 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     4861 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttatctt cgttgggagt
     4921 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     4981 tgtaagtcat tggtcttaaa ggtaccaggc ggggaggcgg cccaaaggga gatccgactc
     5041 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     5101 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     5161 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     5221 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     5281 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     5341 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     5401 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     5461 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     5521 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     5581 taatccagag gttgattgtc gactcaggca ccgggcttgc gggtcatgca ccaggtgcgc
     5641 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
     5701 aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
     5761 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg
     5821 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
     5881 tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg
     5941 aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg
     6001 tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc
     6061 tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg
     6121 gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc
     6181 accgtgggct tgtactcggt catggtggcg atatccgaaa ggcccggaga tgaggaagag
     6241 gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac
     6301 cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt
     6361 cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg
     6421 cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct
     6481 acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact
     6541 aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta
     6601 ccggtggatg tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc
     6661 ggggctgcta aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccttaat
     6721 taattattga tactaaagtt attaaaaaga agattttggt tgattttata aaggtcatag
     6781 aatataatat tttagcatgt gtattttgtg tgctcattta caacgcggcc gcttacttgt
     6841 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     6901 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
     6961 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     7021 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     7081 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     7141 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     7201 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     7261 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     7321 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
     7381 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     7441 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     7501 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc ataccggaag
     7561 ggccgggatt ctcctccacg tcaccgcatg ttagaagact tcctctgccc tcttcgaaca
     7621 tcttggccac gggtttcttc aggatctccc ggatggccct gccgtcgatc ttgccggtca
     7681 ggccctttgg cacttcgtcc acgaatctca cgccgcctct cagccgcttg gcgttggaca
     7741 cctggctggc gacgtagtcc atcacttctt tctcggtcat gttcttgccg gattccagca
     7801 ccaccacggc gccaggcagc tcgccggcca caggatctgg caccccggcc acgccggcgt
     7861 cgaagatgct ggggtgctgc agcaggacgc tttccagctc ggcagggggc acctgatagc
     7921 ccttgtactt gatcaggctc ttcagccggt ccacgatgaa gaagtgcttc tcttcgtcgt
     7981 agtagccgat gtcgccggtg tgcagccagc cctcttcgtc gatcagctct ttggtggcct
     8041 cggggttgtt cacgtagccc ttcatcagca tggggccctt cacgcacact tcgccccgtc
     8101 tgttggggcc caggctcttc ttggtgtcca ggtcgatcac tttggccttg aacaggggca
     8161 ccaccttgcc gctggctcca ggcttgtcgt cgccctcggg ggtgatgatg atggcgctgg
     8221 ttgtctcggt caggccgtag ccctgccgca cgccgggcag attgaaccgc ctggcgacgg
     8281 cctctcccac ttctttgctc aggggggctc cgccgctggc gatctccacc aggttgctca
     8341 ggtcgtactt gttcagcagc tcgctcttgt tcaggatggc gaacagggtg ggcaccagaa
     8401 tcacgtaggt gcacttgtag tcctgcaggg ttttcaggaa ggtttcctcg tcgaacttgg
     8461 tcagcatcac cacccggaag ccgcagatca ggtagcccag ggtggtgaac atgccgaagc
     8521 cgtggtggaa gggcaccacg gtcagcacgg cggtgccggg ggacacctgg ttgccgtaga
     8581 tggggtccct ggcgtggctg aaccgggtca cggtgttctc gtgggtcagc tgcacgccct
     8641 tgggcaggcc ggtgctgccg ctgctgttca tgatcagggc cacctgttct ttccggtcca
     8701 cctccacggt cttgaagctg ctggcctgga agccaggggg ggtgttccgc ttgatgaagg
     8761 tgtccaggca ctggtagccc cggtagtcca ccttgctgtc caggatcacg atggtcttga
     8821 tggtggtcac ggttttctgc acggtgatga ctttgtccag gcccttcttg ctgctgaaca
     8881 cgatggtggg cttgctgatg cccaggctgt gcaccagctc ccgcagggtg tagatctcgt
     8941 tggtgggagc cacgcccacg ccgatgaaca ggccggcgat cacggggatg aagaattcct
     9001 cgcagttctc gctgcacagg gcgatccggc cgtccaccac caggccgtag ttctgcagag
     9061 ccttgcccag gcagcagctc ttttccaggt actcggcgta gctgtagtcc acgccggtca
     9121 cggcgttggt gaaggcaatg gcgcccagct tggcgtatct ttccatgtac ttccgcagct
     9181 gggtgccggc gctgccttcc tcgatggggt agaagggctt ggggcccacc acgatgttct
     9241 cgtcgttttc catattttcc atggtggcgg atccaacgaa tgtcgagagg ctggatcggt
     9301 cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
     9361 tcactaaacg agctctgctt atataggcct cccaccgtac acgcctagct agcgatttag
     9421 agtaatatga aactgattta gagtaatatg aaactgattt agagtaatat gaaactgatt
     9481 tagagtaata tgaaactctc gagactagta tcgataaaat tttgaatttt gtaatttgtt
     9541 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
     9601 tgtacccccc aatcccccct tttcttttaa aagttaaccg ataccgtcga gatccgttca
     9661 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc
     9721 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taa
//
