LOCUS       sequence-460064-        6063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses APEX2-SOPP3 in the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang
            W, Pan W
  TITLE     Photoactivated SOPP3 enables APEX2-mediated proximity labeling with 
            high spatio-temporal resolution in live cells.
  JOURNAL   Cell Res. 2025 Feb;35(2):149-152. doi: 10.1038/s41422-024-01061-9. 
            Epub 2024 Dec 10.
  PUBMED    39653757
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-024-01061-9"; journalName: "Cell Res"; date: 
            "2025-02"; volume: "35"; issue: "2"; pages: "149-152"
COMMENT     Sequence Label: APEX2-SOPP3-TM
FEATURES             Location/Qualifiers
     source          1..6063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        891..909
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     891..908
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     sig_peptide     947..1006
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1016..1042
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2971..3105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2995..3014)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3049..3068
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3218..3238)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3218..3236)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3257..3273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3257..3273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3270..3292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3281..3297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3305..3311,3312..3329,3330..3335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3350..3371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3488..3505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3659..4247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3739..3758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4418..5209,5210..5278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5041..5060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5279..5383)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5409..5864)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5546..5567)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5758..5777
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(5935..6063,1..856)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
       61 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
      121 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
      181 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
      241 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
      301 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
      361 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
      421 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
      481 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
      541 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
      601 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
      661 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
      721 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
      781 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
      841 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
      901 cactatagaa tacaagctac ttgttctttt tgcaggatcc tccaccatgg agacagacac
      961 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgacg ctagctaccc
     1021 atacgatgtt ccagattacg ctgaattcgg gaaatcatac ccaacagtgt ccgcagacta
     1081 ccaggatgcc gtggagaaag ccaagaagaa gctgagaggg tttatcgcag aaaagaggtg
     1141 cgcacctctg atgctgagac tggctttcca cagcgcaggc acctttgaca agggaaccaa
     1201 aacaggcgga ccctttggaa caatcaagca ccctgctgaa ctggcacata gtgccaacaa
     1261 tgggctggac atcgccgtgc ggctgctgga acctctgaaa gcagagttcc caattctgtc
     1321 ctacgccgat ttttatcagc tggcaggagt ggtcgctgtg gaggtcactg ggggccccaa
     1381 ggtgcctttc cacccaggac gggaggacaa accagaacca cctccagagg ggcgcctgcc
     1441 agatccgaca aagggctccg accatctgcg agatgtgttt gggaaagcta tgggcctgac
     1501 tgaccaggat atcgtcgcac tgtctggagg gcacaccatt ggcgccgctc ataaggaaag
     1561 gtcaggcttc gagggaccct ggacaagcaa ccccctgatt ttcgacaatt cttactttac
     1621 tgaactgctg agtggagaga aggaagggct gctgcagctg cccagcgaca aagccctgct
     1681 gtccgatccc gtgttcagac ctctggtcga taagtatgca gccgacgagg atgctttttt
     1741 cgcagattac gcagaagcac atcagaagct gtcagaactg ggatttgccg acgccaccgg
     1801 tggtggaggc ggttcaggcg gaggtggctc tggcggtggc ggatcgggcg gtggcggatc
     1861 gggcggtggc ggatcgggcg gtggcggatc gggcggtggc ggatcgggcg gtggcggatc
     1921 gggcggtggc ggatcgacta gtatggagaa aagtttcgtg ataactgatc cacggctgcc
     1981 agacaatccc atcatcttcg catccgatgg cttcctggag ctgaccgagt attccagaga
     2041 ggagatcctg ggccgcaatg gccgctttct gcagggacca gagacagacc aggccacagt
     2101 gcagaagatt cgcgatgcca ttagagatca gcgcgagatt accgtgcagc tgataaacta
     2161 cacaaaaagc gggaagaaat tcttgaacct cctgaacctc cagcccatta gggaccagaa
     2221 gggtgagctc caggctttca tcggagtggt gctggatgga ctcgagttcc agaaggcctc
     2281 cagcatagtc tataagaaag agggggaaca ggtggagttc tccttcccac tcgcctttac
     2341 agttgaaaag ctgacgggca gtggcgagct gtggtggcag gcggagaggg cttcctcctc
     2401 caagtcttgg atcacctttg acctgaagaa caaggaagtg tctgtaaaac gggttaccca
     2461 ggaccctaag ctccagatgg gcaagaagct cccgctccac ctcaccctgc cccaggcctt
     2521 gcctcagtat gctggctctg gaaacctcac cctggccctt gaagcgaaaa caggaaagtt
     2581 gcatcaggaa gtgaacctgg tggtgatgag agccactcag ctccagaaaa atttgacctg
     2641 tgaggtgtgg ggacccacct cccctaagct gatgctgagc ttgaaactgg agaacaagga
     2701 ggcaaaggtc tcgaagcggg agaaggcggt gtgggtgctg aaccctgagg cggggatgtg
     2761 gcagtgtctg ctgagtgact cgggacaggt cctgctggaa tccaacatca aggttctgcc
     2821 cacatggtcc accccggtgc agccaatggc cctgattgtg ctggggggcg tcgccggcct
     2881 cctgcttttc attgggctag gcatcttctt ctgtgtcagg tgccggcacc gaaggcgcta
     2941 gtctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     3001 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3061 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     3181 gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
     3241 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3301 cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3361 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3421 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3481 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3541 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3601 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3661 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3721 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3781 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3841 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3901 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3961 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4021 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4081 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4141 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4201 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4261 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4321 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4381 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4441 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4501 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4561 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4621 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4681 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4741 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4801 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4861 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4921 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4981 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5041 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5101 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5161 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5221 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5281 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5341 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5401 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5461 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     5521 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     5581 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5641 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5701 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5761 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5821 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     5881 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagtcgac
     5941 cgccaattca atatggcgta tatggactca tgccaattca atatggtgga tctggacctg
     6001 tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc
     6061 agc
//
