LOCUS       sequence-460074-        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed 
            Helical Filament).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-DHF
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1618..1620,1621..2334)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1660..1681)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1921..1940)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2268..2289
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggcgatg agcgacggga gctggaaaag gtcgctgtta aagctataat ggcagcaatg
       61 cttggaaaca ctgacgaagt acgagagcag cttcaacgcg cactggaaat cgccagggag
      121 tcaggcacac tgctggctgt cgtactggct ctcgaagttg tggcacgggt tgctatcgaa
      181 gctgctcgga aaggaaatac tgatgctgta cgggaggctc ttgaagttgc actggaaata
      241 gctcgagaat caggtactaa ggtagccgtt gtgctcgccc tcgaagttgt tgcacgagtc
      301 gcaatagagg ccgcacgacg gggtaacgtg ttggctgtaa tcctcgctct cgaagtagct
      361 cttgagatag cacgcgagtc tggtaccgaa gaggccgccc tcctggcagt tgaagtagta
      421 gtcagagtct cagatgaagc taaaaagcaa ggcaatgcag tcgctgttgc agttgctgaa
      481 caggttgcaa aaaaaattct cgaggaatca ggaggtagtg gcgggtccga gaagccttta
      541 caggtggcca gagtggaaag cagtgtccca caaacaaagg aactgggagg catcaccacc
      601 cttgatgacc ccctaggaca aacgccggaa cttttcgatg cctttatctg ctactgcccc
      661 aacgatatcg agtttgtgca ggagatgatc cggcaactag aacagacaga ctatcggctt
      721 aagttgtgtg tgtccgaccg tgacgtcctg ccgggcacct gtgtctggtc cattgccagc
      781 gagctaattg agaaaaggtg tcgccgcatg gtggtggttg tttctgacga ttatctacag
      841 agcaaggaat gtgacttcca gaccaagttt gcactcagcc tgtctccagg tgtccaacag
      901 aagcgactga ttcctattaa atacaaggcg atgaagaagg actttcccag tatcctgcgg
      961 ttcatcacta tatgcgacta taccaaccct tgcaccaagt cctggttctg gacccgcctt
     1021 gccaaggctt tgtccctgcc cggaggtagt ggcgggtccc ctccttctcc ccaagaaaac
     1081 tcctatgtca gctccactgg tagggctcac tccggagccg caccatggca gccactcgct
     1141 gcaccatcag gtgcttcagc ccaggccgca gagcaactgc aacggggacc caaccagcca
     1201 gtcgagtccg acgaaagtct tggagggctg agcgctgctc tgcggtcctg gcaccttaca
     1261 ccaagttgtc cccttgatcc tgctcctctg cgggaggctg ggtgccccca aggtgacaca
     1321 gcaggggaat cctcatgggg ttcaggacct gggagccggc caaccgctgt tgaaggcctg
     1381 gctttgggct cctctgcatc atcatcatca gaacctcccc agattatcat caatccagct
     1441 cgccagaaaa tggttcagaa attggctttg tatgaggatg gtgccctgga ttccttgcaa
     1501 ttgctgtcat cttcttctct tcccggactt ggacttgaac aagacagaca aggtccagag
     1561 gagtccgacg agttccagag cggaggttcc gggggtactg ggggtagtgg aggaagtgtg
     1621 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1681 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1741 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1801 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1861 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1921 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1981 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2041 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2101 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2161 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2221 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2281 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaa
//
