LOCUS       sequence-460088-        2181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which 
            contains a mutation in the death domain that disrupt oligomer 
            formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-S34Y
FEATURES             Location/Qualifiers
     source          1..2181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1465..1467,1468..2181)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1507..1528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1768..1787)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2115..2136
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtctgcgg gagacccccg cgtgggatcc gggtccctgg actccttcat gttctccata
       61 cccttggtcg cgcttaacgt gggagtgagg cgccgcctat acctgttctt gaaccctcgg
      121 acgcccgtgg cggccgactg gaccttgctg gcggaggaga tgggcttcga gtacttggag
      181 atccgagagc tggaaacgcg ccctgacccc actcgcagtt tgttggatgc ctggcagggg
      241 cgctctggcg cgtctgtcgg caggctgcta gagctgctgg ccttgttaga ccgtgaggat
      301 atactgaagg agctgaagtc gcgcatcgag gaggactgcc agaaatactt aggtaagcag
      361 cagaaccagg agtccgagaa gcctttacag gtggccagag tggaaagcag tgtcccacaa
      421 acaaaggaac tgggaggcat caccaccctt gatgaccccc taggacaaac gccggaactt
      481 ttcgatgcct ttatctgcta ctgccccaac gatatcgagt ttgtgcagga gatgatccgg
      541 caactagaac agacagacta tcggcttaag ttgtgtgtgt ccgaccgtga cgtcctgccg
      601 ggcacctgtg tctggtccat tgccagcgag ctaattgaga aaaggtgtcg ccgcatggtg
      661 gtggttgttt ctgacgatta tctacagagc aaggaatgtg acttccagac caagtttgca
      721 ctcagcctgt ctccaggtgt ccaacagaag cgactgattc ctattaaata caaggcgatg
      781 aagaaggact ttcccagtat cctgcggttc atcactatat gcgactatac caacccttgc
      841 accaagtcct ggttctggac ccgccttgcc aaggctttgt ccctgcccgg aggtagtggc
      901 gggtcccctc cttctcccca agaaaactcc tatgtcagct ccactggtag ggctcactcc
      961 ggagccgcac catggcagcc actcgctgca ccatcaggtg cttcagccca ggccgcagag
     1021 caactgcaac ggggacccaa ccagccagtc gagtccgacg aaagtcttgg agggctgagc
     1081 gctgctctgc ggtcctggca ccttacacca agttgtcccc ttgatcctgc tcctctgcgg
     1141 gaggctgggt gcccccaagg tgacacagca ggggaatcct catggggttc aggacctggg
     1201 agccggccaa ccgctgttga aggcctggct ttgggctcct ctgcatcatc atcatcagaa
     1261 cctccccaga ttatcatcaa tccagctcgc cagaaaatgg ttcagaaatt ggctttgtat
     1321 gaggatggtg ccctggattc cttgcaattg ctgtcatctt cttctcttcc cggacttgga
     1381 cttgaacaag acagacaagg tccagaggag tccgacgagt tccagagcgg aggttccggg
     1441 ggtactgggg gtagtggagg aagtgtgagc aagggcgagg agctgttcac cggggtggtg
     1501 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1561 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1621 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1681 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1741 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1801 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1861 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1921 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1981 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2041 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     2101 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2161 atggacgagc tgtacaagta a
//
