LOCUS       sequence-460092-        2043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains 
            the bacterial death domain from Nostoc. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS_bDD
FEATURES             Location/Qualifiers
     source          1..2043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1327..1329,1330..2043)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1369..1390)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1630..1649)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1977..1998
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgcagacta agattttcat atgtaggcgg ttaactcagg attggcagga cctggctgac
       61 tatttcgaaa tacagcccca cgaacgtgcg gggtttaaac ctggccggga accacatagc
      121 atctgggagt ggctggaaca gaggaatcga cttggtgagc ttgaatctgc tctgatagag
      181 atcggaaggg aggacctggc gcaggagctg aagaagaacg gaggtagtgg cgggtccgag
      241 aagcctttac aggtggccag agtggaaagc agtgtcccac aaacaaagga actgggaggc
      301 atcaccaccc ttgatgaccc cctaggacaa acgccggaac ttttcgatgc ctttatctgc
      361 tactgcccca acgatatcga gtttgtgcag gagatgatcc ggcaactaga acagacagac
      421 tatcggctta agttgtgtgt gtccgaccgt gacgtcctgc cgggcacctg tgtctggtcc
      481 attgccagcg agctaattga gaaaaggtgt cgccgcatgg tggtggttgt ttctgacgat
      541 tatctacaga gcaaggaatg tgacttccag accaagtttg cactcagcct gtctccaggt
      601 gtccaacaga agcgactgat tcctattaaa tacaaggcga tgaagaagga ctttcccagt
      661 atcctgcggt tcatcactat atgcgactat accaaccctt gcaccaagtc ctggttctgg
      721 acccgccttg ccaaggcttt gtccctgccc ggaggtagtg gcgggtcccc tccttctccc
      781 caagaaaact cctatgtcag ctccactggt agggctcact ccggagccgc accatggcag
      841 ccactcgctg caccatcagg tgcttcagcc caggccgcag agcaactgca acggggaccc
      901 aaccagccag tcgagtccga cgaaagtctt ggagggctga gcgctgctct gcggtcctgg
      961 caccttacac caagttgtcc ccttgatcct gctcctctgc gggaggctgg gtgcccccaa
     1021 ggtgacacag caggggaatc ctcatggggt tcaggacctg ggagccggcc aaccgctgtt
     1081 gaaggcctgg ctttgggctc ctctgcatca tcatcatcag aacctcccca gattatcatc
     1141 aatccagctc gccagaaaat ggttcagaaa ttggctttgt atgaggatgg tgccctggat
     1201 tccttgcaat tgctgtcatc ttcttctctt cccggacttg gacttgaaca agacagacaa
     1261 ggtccagagg agtccgacga gttccagagc ggaggttccg ggggtactgg gggtagtgga
     1321 ggaagtgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1381 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1441 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1501 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1561 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1621 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1681 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1741 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1801 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1861 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1921 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     1981 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2041 taa
//
