LOCUS       sequence-460094-        1896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 
            11 TA repeats in place of the death domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-11xTA
FEATURES             Location/Qualifiers
     source          1..1896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1180..1182,1183..1896)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1222..1243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1483..1502)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1830..1851
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgacagcta ccgctactgc cactgctact gccactgcaa ccgccactgc cacagcaact
       61 gcaaccgctg gaggctctgg cggtagcagc gagaagcctt tacaggtggc cagagtggaa
      121 agcagtgtcc cacaaacaaa ggaactggga ggcatcacca cccttgatga ccccctagga
      181 caaacgccgg aacttttcga tgcctttatc tgctactgcc ccaacgatat cgagtttgtg
      241 caggagatga tccggcaact agaacagaca gactatcggc ttaagttgtg tgtgtccgac
      301 cgtgacgtcc tgccgggcac ctgtgtctgg tccattgcca gcgagctaat tgagaaaagg
      361 tgtcgccgca tggtggtggt tgtttctgac gattatctac agagcaagga atgtgacttc
      421 cagaccaagt ttgcactcag cctgtctcca ggtgtccaac agaagcgact gattcctatt
      481 aaatacaagg cgatgaagaa ggactttccc agtatcctgc ggttcatcac tatatgcgac
      541 tataccaacc cttgcaccaa gtcctggttc tggacccgcc ttgccaaggc tttgtccctg
      601 cccggaggta gtggcgggtc ccctccttct ccccaagaaa actcctatgt cagctccact
      661 ggtagggctc actccggagc cgcaccatgg cagccactcg ctgcaccatc aggtgcttca
      721 gcccaggccg cagagcaact gcaacgggga cccaaccagc cagtcgagtc cgacgaaagt
      781 cttggagggc tgagcgctgc tctgcggtcc tggcacctta caccaagttg tccccttgat
      841 cctgctcctc tgcgggaggc tgggtgcccc caaggtgaca cagcagggga atcctcatgg
      901 ggttcaggac ctgggagccg gccaaccgct gttgaaggcc tggctttggg ctcctctgca
      961 tcatcatcat cagaacctcc ccagattatc atcaatccag ctcgccagaa aatggttcag
     1021 aaattggctt tgtatgagga tggtgccctg gattccttgc aattgctgtc atcttcttct
     1081 cttcccggac ttggacttga acaagacaga caaggtccag aggagtccga cgagttccag
     1141 agcggaggtt ccgggggtac tgggggtagt ggaggaagtg tgagcaaggg cgaggagctg
     1201 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1261 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1321 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1381 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1441 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1501 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1561 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1621 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1681 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1741 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1801 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1861 gggatcactc tcggcatgga cgagctgtac aagtaa
//
