LOCUS       sequence-460096-        1920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 
            15 TA repeats in place of the death domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-15xTA
FEATURES             Location/Qualifiers
     source          1..1920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1204..1206,1207..1920)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1246..1267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1507..1526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1854..1875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgacagcca ctgcaaccgc aacagctaca gctaccgcca cagcaaccgc aacagcaacc
       61 gcaactgcta ctgcaacagc aacagcaacc gccggaggat caggcggatc atccgaaaag
      121 cctttacagg tggccagagt ggaaagcagt gtcccacaaa caaaggaact gggaggcatc
      181 accacccttg atgaccccct aggacaaacg ccggaacttt tcgatgcctt tatctgctac
      241 tgccccaacg atatcgagtt tgtgcaggag atgatccggc aactagaaca gacagactat
      301 cggcttaagt tgtgtgtgtc cgaccgtgac gtcctgccgg gcacctgtgt ctggtccatt
      361 gccagcgagc taattgagaa aaggtgtcgc cgcatggtgg tggttgtttc tgacgattat
      421 ctacagagca aggaatgtga cttccagacc aagtttgcac tcagcctgtc tccaggtgtc
      481 caacagaagc gactgattcc tattaaatac aaggcgatga agaaggactt tcccagtatc
      541 ctgcggttca tcactatatg cgactatacc aacccttgca ccaagtcctg gttctggacc
      601 cgccttgcca aggctttgtc cctgcccgga ggtagtggcg ggtcccctcc ttctccccaa
      661 gaaaactcct atgtcagctc cactggtagg gctcactccg gagccgcacc atggcagcca
      721 ctcgctgcac catcaggtgc ttcagcccag gccgcagagc aactgcaacg gggacccaac
      781 cagccagtcg agtccgacga aagtcttgga gggctgagcg ctgctctgcg gtcctggcac
      841 cttacaccaa gttgtcccct tgatcctgct cctctgcggg aggctgggtg cccccaaggt
      901 gacacagcag gggaatcctc atggggttca ggacctggga gccggccaac cgctgttgaa
      961 ggcctggctt tgggctcctc tgcatcatca tcatcagaac ctccccagat tatcatcaat
     1021 ccagctcgcc agaaaatggt tcagaaattg gctttgtatg aggatggtgc cctggattcc
     1081 ttgcaattgc tgtcatcttc ttctcttccc ggacttggac ttgaacaaga cagacaaggt
     1141 ccagaggagt ccgacgagtt ccagagcgga ggttccgggg gtactggggg tagtggagga
     1201 agtgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1261 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1321 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1381 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1441 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1501 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1561 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1621 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1681 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1741 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1801 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1861 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
//
