LOCUS       sequence-460104-        1950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-20xTA with 
            alanine mutation in the TRAF6 binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-20xTA-3xAla
FEATURES             Location/Qualifiers
     source          1..1950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1234..1236,1237..1950)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1276..1297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1537..1556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1884..1905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgactgcta ccgccacagc tactgcaact gccacggcca cagccacggc caccgcgact
       61 gcaactgcaa ccgctactgc tactgccaca gctaccgcaa ctgctactgc cacagcgact
      121 gccggaggat caggcggatc atccgaaaag cctttacagg tggccagagt ggaaagcagt
      181 gtcccacaaa caaaggaact gggaggcatc accacccttg atgaccccct aggacaaacg
      241 ccggaacttt tcgatgcctt tatctgctac tgccccaacg atatcgagtt tgtgcaggag
      301 atgatccggc aactagaaca gacagactat cggcttaagt tgtgtgtgtc cgaccgtgac
      361 gtcctgccgg gcacctgtgt ctggtccatt gccagcgagc taattgagaa aaggtgtcgc
      421 cgcatggtgg tggttgtttc tgacgattat ctacagagca aggaatgtga cttccagacc
      481 aagtttgcac tcagcctgtc tccaggtgtc caacagaagc gactgattcc tattaaatac
      541 aaggcgatga agaaggactt tcccagtatc ctgcggttca tcactatatg cgactatacc
      601 aacccttgca ccaagtcctg gttctggacc cgccttgcca aggctttgtc cctgcccgga
      661 ggtagtggcg ggtcccctcc ttctccccaa gccaactcct atgtcagctc cactggtagg
      721 gctcactccg gagccgcacc atggcagcca ctcgctgcac catcaggtgc ttcagcccag
      781 gccgcagagc aactgcaacg gggacccaac cagccagtcg cgtccgacga aagtcttgga
      841 gggctgagcg ctgctctgcg gtcctggcac cttacaccaa gttgtcccct tgatcctgct
      901 cctctgcggg aggctgggtg cccccaaggt gacacagcag gggaatcctc atggggttca
      961 ggacctggga gccggccaac cgctgttgaa ggcctggctt tgggctcctc tgcatcatca
     1021 tcatcagaac ctccccagat tatcatcaat ccagctcgcc agaaaatggt tcagaaattg
     1081 gctttgtatg aggatggtgc cctggattcc ttgcaattgc tgtcatcttc ttctcttccc
     1141 ggacttggac ttgaacaaga cagacaaggt ccagaggcgt ccgacgagtt ccagagcgga
     1201 ggttccgggg gtactggggg tagtggagga agtgtgagca agggcgagga gctgttcacc
     1261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1441 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1681 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     1861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1921 actctcggca tggacgagct gtacaagtaa
//
