LOCUS       sequence-460226-        2813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO3s
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..223
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             271..1221
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELTTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     1222..1238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1222..1238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1561..1578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1561..1577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1896..2687
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAAYWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2688..2707
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2688..2706
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2795..2813)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gacgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactccatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa caacactaga gaacatactg
     1201 gctaaatacg gaaggatctg acaggaaaca gctatgacca tgcccgggta agtgactagc
     1261 ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga ccaaaggctc
     1321 cgaagagccg agtgtcttcg gttcgagacc gaattcgcga gctctcgcta gccagtttaa
     1381 acaattgcgg ccgcgtacta gtctatcgat ttggatccac cctgcaggaa gtctagaccg
     1441 tcgacacggt ctctgagaga agacacgtcg ctcttcgacg taagtaactg actcggtacc
     1501 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctaagctta
     1561 actggccgtc gttttacacc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     1621 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1681 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1741 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     1801 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1861 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag
     1921 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1981 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2041 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2101 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2161 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2221 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2281 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2341 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2401 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2461 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2521 cctactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2581 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2641 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataataa tacgactcac
     2701 tatagggtaa ctaattgagg cgcgcctacc accgtcaaaa aaaacggcgc tttttagcgc
     2761 cgtttttatt tttcaacctt cctaggtaag tgacccttta gtgagggtta att
//
