LOCUS       sequence-460234-       11224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP tagged DDHD2 lipase in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Kumar M, Wu Y, Knapp J, Pontius CL, Park D, Witte RE, McAllister R, 
            Gupta K, Rajagopalan KN, De Camilli P, Ryan TA
  TITLE     Triglycerides are an important fuel reserve for synapse function in 
            the brain.
  JOURNAL   Nat Metab. 2025 Jul;7(7):1392-1403. doi: 10.1038/s42255-025-01321-x.
            Epub 2025 Jul 1.
  PUBMED    40595405
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-025-01321-x"; journalName: "Nat Metab"; date: 
            "2025-07"; volume: "7"; issue: "7"; pages: "1392-1403"
COMMENT     Sequence Label: hSyn-eGFP-DDHD2
FEATURES             Location/Qualifiers
     source          1..11224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2541..2988
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3054..3767
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3054..3056,3057..3767)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3096..3117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3357..3376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3704..3725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6031..6619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6084..6104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6502..6513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7026..7206
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7232..7249)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7238..7462
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7508..7936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7595..7614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7805..7826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7945..7965)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7950..8279
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8130..8265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8192..8211
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8327..8374
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8393..8767
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8897..9018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8934..8953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8988..9007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9067..9083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9067..9083)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9080..9102)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9091..9107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9115..9121,9122..9139,9140..9145))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9160..9181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9298..9315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9469..10057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9549..9568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10228..11019,11020..11088))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10851..10870
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11089..11193)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgctagac
     2521 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
     2581 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2641 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2701 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2761 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     2821 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2881 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     2941 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gatctagacg
     3001 ataagggcga attctgcaga tatccatcac actggcggcc gctcgaggcc accatgagca
     3061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3721 tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagggt accatgtcat
     3781 cagtgcagtc acaacaggag cagttgtccc agtcagatcc atctccgtca ccaaactcat
     3841 gtagttcctt tgagctaata gacatggatg ctggcagctt gtatgaacca gtttctcccc
     3901 attggtttta ttgtaagata atagattcta aggagacatg gattcctttc aactctgagg
     3961 attcacagca gctggaagag gcatatagct ctggaaaagg ttgtaatggg agagttgttc
     4021 ctactgatgg gggcagatat gatgttcatt tgggggagag gatgcggtat gctgtatact
     4081 gggatgaact ggcatcggaa gtgagacgat gtacgtggtt ttacaagggg gacaaagaca
     4141 ataagtatgt tccctactcg gagagcttca gccaagtttt agaggaaact tacatgcttg
     4201 ctgtaacttt ggatgaatgg aaaaagaaac tggaatctcc caacagagaa attattattt
     4261 tacacaatcc aaagcttatg gtgcattacc agccagttgc agggtctgat gattggggtt
     4321 caacacccac ggagcagggt cgaccaagaa ctgtgaagag aggagttgag aacatctctg
     4381 ttgacattca ttgtggagaa cctttacaaa tagatcactt ggtttttgta gtccatggga
     4441 ttggaccagc ttgtgatctc cgctttcgaa gcattgtaca gtgtgttaat gattttcgca
     4501 gtgtttcctt gaacttgcta cagacacatt ttaagaaagc ccaagaaaat cagcagattg
     4561 ggagggtaga atttcttcca gtcaactggc acagtccttt gcattctact ggtgtggatg
     4621 tagatctgca gcgaataacc ctgcccagca ttaaccgcct caggcacttc accaatgaca
     4681 caattctgga tgtcttcttc tacaatagtc ccacctactg tcagactatt gtggacacag
     4741 ttgcttctga aatgaaccga atatacacac tttttctaca gaggaaccct gatttcaaag
     4801 ggggtgtatc cattgctggt catagtttag gttcgcttat attgtttgat atcctaacaa
     4861 atcagaaaga ttctttgggg gatattgaca gtgaaaagga ttcgctaaat attgtaatgg
     4921 atcaaggaga tacacctaca ctagaggaag atttgaagaa acttcagctc tctgaattct
     4981 ttgatatctt tgagaaggag aaagtagata aggaagctct ggctttatgt acagaccgag
     5041 atcttcagga aataggaatt cctttaggac caagaaagaa gatattaaac tatttcagca
     5101 ccagaaaaaa ctcaatgggt attaagagac cagccccgca gcctgcttca ggggcaaaca
     5161 tccccaaaga atctgagttc tgcagtagca gtaatactag aaatggtgac tatctggatg
     5221 ttggcattgg gcaggtgtct gtgaaatacc cccggctcat ctataaacca gagatattct
     5281 ttgcctttgg atctcccatt ggaatgttcc ttactgtccg aggactaaaa agaattgatc
     5341 ccaactacag atttccaacg tgcaaaggtt tcttcaatat ttatcaccct tttgatcctg
     5401 tggcctatag gattgaacca atggtggtcc caggagtgga atttgagcca atgctgatcc
     5461 cacatcataa aggcaggaag cggatgcact tagaactgag agagggcttg accaggatga
     5521 gtatggacct taagaacaac ttgctaggtt cgctgcggat ggcctggaag tcttttacca
     5581 gagctccata ccctgcctta caagcttcag aaacaccaga agaaactgaa gcagaacctg
     5641 aatcaacttc agagaagcct agtgatgtta acacagaaga gacctctgtg gcagttaaag
     5701 aagaagtcct gcctatcaat gtggggatgc tgaatggagg ccaacgcatt gactatgtgc
     5761 tacaggagaa gcctattgaa agttttaatg agtatttatt tgctttacaa agccatctat
     5821 gctactggga gtctgaagat acagtattgc tcgtcctcaa agagatctac caaacccagg
     5881 gtatcttcct tgatcagcct ttacagtaag tttaaacctg gaaaaacatg gagcaatcac
     5941 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6001 ggaggtgggt tttccagtca cacctcagac aatcaacctc tggattacaa aatttgtgaa
     6061 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6421 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6601 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     6661 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6721 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     6781 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     6841 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     6901 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     6961 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     7021 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7081 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7141 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7201 ctagcagggc cgctttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     7261 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7321 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7381 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7441 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     7501 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7561 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7741 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7801 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7861 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7921 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7981 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     8041 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8101 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8161 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     8221 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8281 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     8341 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     8401 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     8461 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     8521 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     8581 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     8641 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     8701 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     8761 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8821 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8881 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8941 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9001 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     9061 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9901 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11221 cgtc
//
