LOCUS       sequence-460238-       11244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Halo tagged CPT2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Kumar M, Wu Y, Knapp J, Pontius CL, Park D, Witte RE, McAllister R, 
            Gupta K, Rajagopalan KN, De Camilli P, Ryan TA
  TITLE     Triglycerides are an important fuel reserve for synapse function in 
            the brain.
  JOURNAL   Nat Metab. 2025 Jul;7(7):1392-1403. doi: 10.1038/s42255-025-01321-x.
            Epub 2025 Jul 1.
  PUBMED    40595405
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-025-01321-x"; journalName: "Nat Metab"; date: 
            "2025-07"; volume: "7"; issue: "7"; pages: "1392-1403"
COMMENT     Sequence Label: hSyn-hCPT2-Halo
FEATURES             Location/Qualifiers
     source          1..11244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2541..2988
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3048..3057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6051..6639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6104..6124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6522..6533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7046..7226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7252..7269)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7258..7482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7528..7956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7615..7634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7825..7846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7965..7985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7970..8299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8150..8285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8212..8231
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8347..8394
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8413..8787
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8917..9038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8954..8973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9008..9027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9087..9103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9087..9103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9100..9122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9111..9127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9135..9141,9142..9159,9160..9165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9180..9201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9318..9335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9489..10077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9569..9588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10248..11039,11040..11108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10871..10890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11109..11213)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgctagac
     2521 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
     2581 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2641 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2701 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2761 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     2821 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2881 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     2941 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gatctagacg
     3001 ataagggcga attctgcaga tatccatcac actggcggcc gctcgaggcc accatggtgc
     3061 cccgcctgct gctgcgcgcc tggccccggg gccccgcggt tggtccggga gcccccagtc
     3121 ggcccctcag cgccggctcc gggcccggcc agtacctgca gcgcagcatc gtgcccacca
     3181 tgcactacca ggacagcctg cccaggctgc ctattcccaa acttgaagac accattagga
     3241 gatacctcag tgcacagaag cctctcttga atgatggcca gttcaggaaa acagaacaat
     3301 tttgcaagag ttttgaaaat gggattggaa aagaactgca tgagcagctg gttgctctgg
     3361 acaaacagaa taaacataca agctacattt cgggaccctg gtttgatatg tacctatctg
     3421 ctcgagactc cgttgttctg aactttaatc catttatggc tttcaatcct gacccaaaat
     3481 ctgagtataa tgaccagctc acccgggcaa ccaacatgac tgtttctgcc atccggtttc
     3541 tgaagacact ccgggctggc cttctggagc cagaagtgtt ccacttgaac cctgcaaaaa
     3601 gtgacactat caccttcaag agactcatac gctttgtgcc ttcctctctg tcctggtatg
     3661 gggcctacct ggtcaatgcg tatcccctgg atatgtccca gtattttcgg cttttcaact
     3721 caactcgttt acccaaaccc agtcgggatg aactcttcac tgatgacaag gccagacacc
     3781 tcctggtcct aaggaaagga aatttttata tctttgatgt cctggatcaa gatgggaaca
     3841 ttgtgagccc ctcggaaatc caggcacatc tgaagtacat tctctcagac agcagccccg
     3901 cccccgagtt tcccctggca tacctgacca gtgagaaccg agacatctgg gcagagctca
     3961 ggcagaagct gatgagtagt ggcaatgagg agagcctgag gaaagtggac tcggcagtgt
     4021 tctgtctctg cctagatgac ttccccatta aggaccttgt ccacttgtcc cacaatatgc
     4081 tgcatgggga tggcacaaac cgctggtttg ataaatcctt taacctcatt atcgccaagg
     4141 atggctctac tgccgtccac tttgagcact cttggggtga tggtgtggca gtgctcagat
     4201 tttttaatga agtatttaaa gacagcactc agacccctgc cgtcactcca cagagccagc
     4261 cagctaccac tgactctact gtcacggtgc agaaactcaa cttcgagctg actgatgcct
     4321 taaagactgg catcacagct gctaaggaaa agtttgatgc caccatgaaa accctcacta
     4381 ttgactgcgt ccagtttcag agaggaggca aagaattcct gaagaagcaa aagctgagcc
     4441 ctgacgcagt tgcccagctg gcattccaga tggccttcct gcggcagtac gggcagacag
     4501 tggccaccta cgagtcctgt agcactgccg cattcaagca cggccgcact gagaccatcc
     4561 gcccggcctc cgtctataca aagaggtgct ctgaggcctt tgtcagggag ccctccaggc
     4621 acagtgctgg tgagcttcag cagatgatgg ttgagtgctc caagtaccat ggccagctga
     4681 ccaaagaagc agcaatgggc cagggctttg accgacactt gtttgctctg cggcatctgg
     4741 cagcagccaa agggatcatc ttgcctgagc tctacctgga ccctgcatac gggcagataa
     4801 accacaatgt cctgtccacg agcacactga gcagcccagc agtgaacctt gggggctttg
     4861 cccctgtggt ctctgatggc tttggtgttg ggtatgctgt tcatgacaac tggataggct
     4921 gcaatgtctc ttcctaccca ggccgcaatg cccgggagtt tctccaatgt gtggagaagg
     4981 ccttagaaga catgtttgat gccttagaag gcaaatccat caaaagtctc gagcatgcat
     5041 ctagagaaat cggcaccggc ttccctttcg acccccacta cgtggaagtg ctgggcgaga
     5101 gaatgcacta tgtggacgtg ggccctaggg acggaacccc cgtgctgttt ctgcacggca
     5161 accccaccag cagctacgtg tggcggaaca tcatccccca cgtggccccc acccacagat
     5221 gtatcgcccc tgacctgatc ggcatgggca agagcgacaa gcccgacctg ggctacttct
     5281 tcgacgacca cgtgcggttc atggacgcct tcatcgaggc cctgggcctg gaagaggtgg
     5341 tgctcgtgat ccacgattgg ggctctgccc tgggcttcca ctgggccaag agaaaccccg
     5401 agagagtgaa gggaatcgcc ttcatggaat tcatcagacc catccccacc tgggacgagt
     5461 ggcccgagtt cgccagagag acattccagg ccttcagaac caccgacgtg ggcagaaagc
     5521 tgatcatcga ccagaacgtg ttcatcgaag gcaccctgcc tatgggcgtc gtgcggcctc
     5581 tgaccgaggt ggaaatggac cactacagag agccctttct gaaccccgtg gaccgcgaac
     5641 ctctgtggcg gttccctaac gagctgccta tcgctggcga gcccgccaac attgtggccc
     5701 tggtggaaga gtacatggac tggctgcacc agagccccgt gcccaagctg ctgttttggg
     5761 gaacacccgg cgtgctgatc cctcctgctg aggctgctag actggccaag agcctgccca
     5821 actgcaaggc cgtggatatc ggccctggcc tgaacctgct gcaggaagat aaccccgatc
     5881 tgatcggctc cgagatcgcc cggtggctga gcaccctgga aatagctagg tttaaacctg
     5941 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6001 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcagac aatcaacctc
     6061 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6121 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6181 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6241 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6301 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6361 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6421 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6481 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6541 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6601 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc
     6661 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6721 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6781 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     6841 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     6901 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6961 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     7021 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     7081 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7141 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7201 ttagtcagtg tggaaaatct ctagcagggc cgctttaaac ccgctgatca gcctcgactg
     7261 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7321 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7381 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7441 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     7501 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     7561 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7621 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7681 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7741 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7801 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7861 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7921 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7981 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8041 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8101 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8221 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     8281 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8341 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8401 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     8461 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     8521 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     8581 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     8641 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     8701 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     8761 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     8821 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8881 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8941 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9001 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     9061 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9181 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9241 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    10021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11221 ccccgaaaag tgccacctga cgtc
//
