LOCUS       sequence-460239-       21654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of HISPS under the copper inducible 
            CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin 
            promoter and the constitutive expression of LUZ, CPH,EGFP and p19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21654)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 21654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4635
FEATURES             Location/Qualifiers
     source          1..21654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5478..5730
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      7583..7835
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8841..9185
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(9555..9566)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      10086..10338
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11182..11526
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             11654..11662
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      12544..12796
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        13640..13984
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(14058..14060,14061..14063,14064..14774)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(14058..14060,14061..14063,14064..14774)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14103..14124)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(14364..14383)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14711..14732
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      14810..15062
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    15303..15327
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16628..17257
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             17686..18759
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      18825..19019
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(19255..19277)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    19363..19503
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     19418..19437
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(19518..19535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19689..20277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19769..19788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(20365..21180)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(20365..21180)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     21088..21107
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    21613..21637
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaatg
      361 aactcaagta aaaatcctcc ttctactctc cttgatgttt tcttggatac cgcaagaaac
      421 ttggatacag caagcagaaa tgtgctcgag tgtggtgaac atcgatggag ttacagagaa
      481 ctggatacag tgtcctcagc tctcgcccag catcttcgat acacggtggg gctgtcccct
      541 actgtcgcag tgatcagcga gaatcacccg tacatattgg ccctcatgct cgctgtatgg
      601 aaattgggcg gtaccttcgc tccgatagat gtccacagcc ctgcagaact ggttgccggg
      661 atgcttaaca tagtctcacc gtcttgcctg gtcatcccgt catcagacgt aacaaaccag
      721 acccttgcat gtgatcttaa cattccagtg gttgcatttc atcctcacca aagcacaatc
      781 ccggaactga acaaaaagta cctgacggac agtcaaatca gccctgatct cccattcccc
      841 gatcccaacc gtccggctct ttacctgttc acttcttctg ctacgagtag atccaacttg
      901 aagtgcgtgc ccctgaccca caccttcata ctgagaaata gcctgtcaaa gagggcctgg
      961 tgcaaaagaa tgaggccaga aacagatttc gatggtataa gggttttagg atgggcacca
     1021 tggagtcatg ttctggctca catgcaagat atagggcccc ttacactcct gaatgctgga
     1081 tgttacgtct ttgcaacgac accgagcaca tatccaaccg agttgaagga cgatagggat
     1141 gtcatttctt gcgccgcaaa tgcagttatg tacaaggggg ttaagtcctt cgcctgtctc
     1201 ccgtttgtgc tgggaggcct taaggccctg tgtgagtccg agccgtccgt caaagcacaa
     1261 ttacaggtcg aggagcgagc acagttgtta aagagcttac aacacatgaa gattcttgaa
     1321 tgtggtggcg ctatgctcga agtttcagtg gcttcttggg ctatcgagaa tcgtattccc
     1381 attagtatag gaatcggtat gaccgaaact ggcggagcct tgttcgctgg acccgttcaa
     1441 gcaatccaaa cgggatttag ttcagaggac aagtttattg aggatgccac atatctcctt
     1501 gtcaaagacg attacgagtc acacgcagaa gaggacatca atgaaggcga actggttgtt
     1561 aaaagccgaa tgctgccaag gggttatctg gggtataacg atccatcctt ctcagtggac
     1621 gatgcagggt gggttacctt caaaacaggc gacaggtact ccgtgacccc ggatggtaag
     1681 ttttcttggt taggaaggaa tacagacttt atacagatga caagcggtga gactctcgat
     1741 cccaggccca tcgaaagttt gctttgcgag tcctccctta tatcaagagc ctgtgtaata
     1801 ggcgataagt tcctgaatgg gcccgccaca gcagtgtgtg ccataataga attggagccg
     1861 actacggtag agaaaggtca ggcccatagc agggacatag cccgaatttt tgcccccata
     1921 aaccgtgatt tgcctcctcc tttaagaatc gcctggagcc acgtcctcgt gctccaaccc
     1981 agtgaaaaaa taccgatgac caaaaaagga acgatcttcc gtaagaagat cgaacaagtc
     2041 tttggtagtg cacttggtgg gtcctcaggt gacaattccc aagcaaccac tgatgcatca
     2101 gtcgtccgtc gagatgaact gtcaaacact gttaagcata tcatatcacg tgttctgggc
     2161 gtgagcgacg acgagttgct gtggactctc tcatttgccg aacttggaat gacctccgcc
     2221 cttgcaacga gaattgccaa tgaattgaat gaggttttag taggggtcaa tctgcctatc
     2281 aatgcatgtt atatacacgt tgacttacca tccctgtcaa atgcagtata tgccaaactg
     2341 gctcacttga aactccctga cagaacacca gagccgcgta aggcaccggt tgagaatcct
     2401 ggcggaaaag agatcgtaat agtgggacaa gcatttagac ttccaggctc tattaatgac
     2461 gtggctagtc tccgtgacgc ttttctggcc cgtcaagcaa gttcaatcat tacagaaata
     2521 ccaccagaca gatgggatca cgcatccttc tacccgaagg acatacgttt caacaaggcc
     2581 ggcttagtgg acattgctaa ctacgaccac agtttcttcg gcttgacggc tacagaagct
     2641 ctttacttat ctccgaccat gagactcgcc ctggaagtta gttttgaggc ccttgagaac
     2701 gcaaacattc cggtttctca gctgaagggg tcacaaacag ctgtctacgt tgctaccacg
     2761 gatgacgggt tcgagactct cttaaatgca gaggctgggt acgacgctta cacccgattc
     2821 tacggtacgg ggcgtgcagc ttccacggca tccggcagga tctcatacct gcttgacgtt
     2881 catggaccca gtataactgt ggacacggca tgtagtggag gggctgtttg cattgatcaa
     2941 gctatagact acctgcaatc cagttcagcc gctgataccg ccatcatttg tgcttctaat
     3001 actcattgct ggccaggatc attcatgttt ctgagcgccc agggtatggt aagctccggc
     3061 ggacgatgcg ctactttcac gaccgatgcc gacggctacg tacctagcga gggtgcagtc
     3121 gccttcattc ttaaaacacg tgaagcagct atgagggaca aagacacaat tctggctacc
     3181 atcaaagcaa cacaaatttc tcataatggt aggtcccagg gattagtcgc ccccaatgta
     3241 aattcccaag ccgacttgca tagaagcctg ctccaaaaag ccgggctttc accggccgac
     3301 attcatttca tcgaggcaca cggaactgga acctccctcg gagatctgtc cgagatccaa
     3361 gctataaatg atgcatatac gagctcacaa cctcgaacgg ccggcccgct gatcgtttct
     3421 gcaagcaaga cagtgatagg acacactgaa ccagccggtc cgttggtggg gatgctgtca
     3481 gtactgaact cctttaaaga gggggccgtc cctggtttgg cacacttgac cgccgataac
     3541 ctgaatcccg ctcttgattg cagcagtgtt ccgttactca taccttacca gcccgttcac
     3601 ctggcagccc ccaagcctca tcgtgctgct gtaatgtcat atggattctc tggcaccctc
     3661 ggtgggatcg tgctggaggc accagatgag gaaagactcg aagaggaacc tcctaatgac
     3721 aaacctatgt tgtttgtagt tagtgcaaaa acacatactg cattaataga atatctcggc
     3781 cgataccttg aattcctctt gcaggcaaat ccacaagact tctgcgatat ttgttatact
     3841 tcatgcgttg ggcgtgaaca ttatcgatac agattcgcat gcgtagccaa cgacatggag
     3901 gatttaatag gacaattgca gaagcgttta ggctctaagg tcccaccgaa accttcctac
     3961 aaaagagggg cccttgcatt cgccttcagc ggtcagggca cacagttccg agggatggct
     4021 accgagctcg caaaagccta ttccggattc cgaaaaatag tctctgatct cgctaagagg
     4081 gcaagtgaac tctcaggtca cgctattgat cgatttttgt tagcttacga tattggggct
     4141 gaaaacgtcg ccccggatag cgaggctgat caaatatgta tcttcgtata tcaatgtagc
     4201 gtgcttcgtt ggcttcagac aatgggcatt agaccatctg ctgttatagg gcactccctc
     4261 ggtgagatta gcgcttctgt cgccgccggt gcattgtctc tggactctgc actggacctt
     4321 gttatcagtc gagctcgact gttaaggagc tctaccaatg cacccgccgg tatggcagcc
     4381 atgtcagcat cacaggacga agttgttgag ttgattggga aattagacct tgataaagca
     4441 aattcactct ctgtttctgt tatcaatggt cctcagaata cggtagtaag cggttcttca
     4501 gcagccattg aaagtatagt cgcactggcc aaagggcgaa agataaaagc aagtgctctc
     4561 aacatcaacc aagccttcca ctctccctac gtagattctg cagtaccagg tttgcgtgct
     4621 tggtcagaga agcatatttc ctctgctaga ccattacaga tccccctgta ctctacactc
     4681 cttggcgccc aagtaagtga gggacaaatg ctgaatccgg atcattgggt agatcacgca
     4741 agaaaacctg tccaattcgc tcaggccgcc accattatga aagaaagctt taccggagta
     4801 atcattgaca taggtcctca agtagttgct tggagtttgc tgctctcaaa cggactgaca
     4861 tcagttaccg ctctcgccgc aaagcgagga cgatcacagc aggttgcctt ccttagtgct
     4921 cttgcagatt tataccaaga ctatggtgtc gtgcctgact ttgtgggatt atacgctcaa
     4981 caggaggacg ccagtcgttt aaagaaaacc gatattctga cctatccttt ccaaagggtg
     5041 agaaggtatc catctttcat cccgagtcgt agggcaccta ctcatgccca tgtccaagat
     5101 gaggaaacat tgtcatctgg atcttccaca cccacattgg agaatacaga tctcgactcc
     5161 ggtaaagagt ctttgatggg acccacacgt ggccttcttc gagtagacga tctgagagac
     5221 agtatcgttt cttctgtaaa ggatgtactg gagctgaaat caaatgagga cttggacttg
     5281 tccgagtcct tgaacgcatt gggaatggac agtattatgt tcgctcaatt acgtaaaagg
     5341 ataggggagg gactcgggtt atccgtcccg atggtattcc tgtctgacgc cttctcaatc
     5401 ggcgagatgg ttagtaacct tgtagaacag gcagaggcta gtgaggacaa ctgagcttgg
     5461 aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5521 ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     5581 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     5641 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     5701 cgcgcgcggt gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagacga
     5761 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5821 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5881 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg
     5941 gaccgactcg cgctgtcagg agaaaagctt cgaagtttaa acgattgaat ataaccgacg
     6001 tgactgttac atttagggat tgaatataac cgacgtgact gttacattta gggattgaat
     6061 ataaccgacg tgactgttac atttagggat tgaatataac cgacgtgact gttacattta
     6121 gggatgctag ctcccgtcct aatacctcta cgtgcaagca gtataaaaac aaaatacata
     6181 aacataacta aaatctcata tcgttgtaat tgtcatatac tagtggtaat taagtccagt
     6241 tacacttaag gttaaatttg ctgactcttt tttacctttc atttctgaaa atggcatcct
     6301 ttgaaaatag tctgtccgtt ttgatcgtcg gggctggcct tgggggatta gcagccgcaa
     6361 tagctcttag gagacagggc catgtagtaa agatctatga tagttcctca ttcaaagctg
     6421 aactgggtgc tggccttgct gtacctccga atacgcttag aagccttcaa cagttaggtt
     6481 gtaatacaga gaatttgaac ggagtcgaca atctctgctt cacggccatg ggttacgacg
     6541 gctccgtagg tatgatgaat aacatgacag actacaggga ggcttatggc acatcctgga
     6601 ttatggttca tcgagtggat ttacataatg aacttatgcg tgtcgcactt gatccggggg
     6661 ggctgggacc accagcaaca ttacatttaa atcacagagt gaccttctgc gacgttgacg
     6721 catgtacggt aacttttacc aacgggacga cacaaagtgc cgacttgatc gtaggggctg
     6781 acggcatacg aagcaccata cgtagattcg tcttggagga agatgtaact gtgcctgcca
     6841 gcgggatcgt cggtttcaga tggctggtgc aagccgatgc cttagaccct taccctgagt
     6901 tagattggat tgttaagaag cctccactcg gtgctcgtct gatctctacg ccacagaatc
     6961 cacagtccgg cgtggggttg gctgaccgtc gtacaattat catctacgct tgtaggggtg
     7021 gcacgatggt taatgtgttg gctgtccacg acgacgaaag agatcagaat acggcagatt
     7081 ggtcagtgcc ggcttccaaa gatgatctct tccgtgtatt ccatgactac cacccaagat
     7141 tcagacgttt gctcgaactt gctcaagata tcaatctctg gcaaatgagg gtagttcccg
     7201 tgcttaaaaa atgggtgaac aagcgtgtct gtttattagg agatgccgcc catgcttctc
     7261 tccccacgct gggtcaaggg ttcggaatgg gactggagga cgccgtggca ctcggcacat
     7321 tgttacccaa aggaactacg gcttcacaga tcgaaacgag actggccgtt tacgagcaat
     7381 tacgaaagga tagggctgag ttcgtagcag cagaatccta cgaggaacag tatgtccctg
     7441 agatgcgtgg gctctatctt cgttccaaag aactccgaga tcgagtgatg ggatatgata
     7501 ttaaagtgga gtcagaaaag gttttggaga ctctgcttcg atcctctaat tctgcatgag
     7561 cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     7621 gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt acgttaagca
     7681 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     7741 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     7801 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgccaagct aattcttgaa
     7861 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7921 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7981 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8041 aatgggaccg actcgcgctg tcaggagtct caatggtaac tttactcttt atttaaccat
     8101 acattttttt ttattttttt cactttgttc ttcatccact attgttcttt gttcatcttg
     8161 aacaaaagct ccctccttct ttgttcttca tccaccattg ttcttcatca atcatttcgc
     8221 tgtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat ggacgacttt
     8281 ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc
     8341 accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     8401 agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa tctccaggag
     8461 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
     8521 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
     8581 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt
     8641 cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc
     8701 gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc
     8761 ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     8821 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     8881 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
     8941 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ccctgccgac
     9001 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     9061 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     9121 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     9181 aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac aacattacaa
     9241 tttactattc tagtcgaaat gagaattaac atctcccttt catcattatt tgaacgtctg
     9301 tcaaagttat ccagcagatc cattgcaatc acctgtggtg tcgtcttggc ctccgcaatc
     9361 gcttttccga taatccgtag ggactatcag acttttttgg aagtcgggcc gagttacgct
     9421 ccgcagaatt tcagaggtta catcatagta tgcgtgcttt cactctttag gcaggaacaa
     9481 aaagggttgg ctatctatga tcgactgccc gaaaagcgaa ggtggttggc agatctccca
     9541 ttccgagaag gtacgcgtcc ttctatcaca agtcatatca tccaaagaca gaggactcag
     9601 ctggtagacc aagaatttgc aacccgagaa ctcattgata aagttatacc cagagttcag
     9661 gctcgacaca ccgataagac gtttctgtca acctcaaagt tcgaatttca tgccaaagca
     9721 atttttttgc tgcccagtat tcccatcaat gatcccttga atataccttc acatgatact
     9781 gtcagacgaa caaaaaggga gatcgctcac atgcacgatt accacgactg caccctgcac
     9841 ttggctctcg cagcccagga cggtaaagaa gtacttaaaa aagggtgggg ccagagacat
     9901 cccctcgctg gccccggcgt gccgggacct ccaaccgaat ggacgttttt atatgcaccg
     9961 cgtaacgagg aggaagccag agtggtcgag atgatagtgg aggccagtat tggttacatg
    10021 acaaatgacc ccgctggtaa aattgtagag aacgctaagt gagcttggaa tggatcttcg
    10081 atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    10141 ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    10201 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    10261 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    10321 catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg
    10381 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    10441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg
    10561 ctgtcaggag actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt
    10621 tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac
    10681 caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
    10741 tagagaggcc tacgcggcag gtctgatcaa gacgatctac ccgagtaata atctccagga
    10801 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
    10861 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
    10921 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt
    10981 tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc
    11041 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
    11101 cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    11161 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    11221 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    11281 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga
    11341 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    11401 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    11461 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    11521 gaggactccg gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca
    11581 atttactatt ctagtcgaaa tggccccaat aagttccacg tggtctaggc tcataaggtt
    11641 tgtggctgtg gaaacctcct tagttcacat cggcgagcct attgatgcaa caatggacgt
    11701 gggcttagca cgtagggaag ggaagacaat acaagcctat gagatcatcg gcagtggctc
    11761 tgctttagat ctgtctgccc aagtgtcaaa gaacgtactt acggtccgtg agctcttaat
    11821 gcccttgagc cgtgaggaga ttaaaacggt aagatgcctt ggccttaatt atcccgtcca
    11881 cgccacagaa gccaacgtgg ccgtgccgaa attcccgaac cttttttaca aacccgtaac
    11941 ctccctcatc ggaccaggtg ggttgataac catcccttca gttgtccaac cacctaaaga
    12001 gcaccagagc gattacgaag ccgagttggt tatcgtcatc ggtaaagccg ccaaaaatgt
    12061 cagtgaagat gaagctctgg actacgtgtt gggctacacc gcagccaatg acatctcatt
    12121 taggaaacat cagttggccg tttctcaatg gtcattctcc aagggttttg atggcacgaa
    12181 tcccctggga ccgtgcctgg tttctgcttc ctctattcca gatccacagg atattccgat
    12241 ccaatgtaaa ctcaacggtg gggtggtgca aaatggaaac actcgtgatc agatttttaa
    12301 tgtcaagaaa acaatctctt ttttaagcca agggactacg ttggagcctg gttccataat
    12361 cctgacgggg acgcccgacg gagtgggctt cgtaagaaac ccaccgcttt atttgaagga
    12421 cggtgatgaa gtaatgactt ggatcggatc aggtatagga accttagcca acactgtccg
    12481 agaggagcag acttgtttcg cttcaggagg gcacgagtaa gcttggaatg gatcttcgat
    12541 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
    12601 gcgacgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
    12661 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
    12721 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    12781 tctatgttac tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat
    12841 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    12901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    12961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct
    13021 gtcaggagac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
    13081 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
    13141 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
    13201 gagaggccta cgcggcaggt ctgatcaaga cgatctaccc gagtaataat ctccaggaga
    13261 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
    13321 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
    13381 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
    13441 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
    13501 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
    13561 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    13621 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
    13681 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
    13741 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca
    13801 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    13861 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
    13921 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
    13981 ggactccggt atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat
    14041 ttactattct agtcgaaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
    14101 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
    14161 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    14221 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
    14281 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
    14341 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
    14401 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
    14461 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
    14521 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
    14581 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
    14641 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
    14701 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
    14761 acgagctgta caaggacgag ctgtaagctt ggaatggatc ttcgatcccg atcgttcaaa
    14821 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga cgattatcat
    14881 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
    14941 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
    15001 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
    15061 tcgggaattg ccaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
    15121 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    15181 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    15241 gacaataacc ctgataaatg cttcaataat gggaccgact cgcgctgtca tgagacgaat
    15301 tctgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
    15361 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
    15421 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
    15481 ggcttctgac gttcagtgca gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt
    15541 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
    15601 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
    15661 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
    15721 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
    15781 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
    15841 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
    15901 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
    15961 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
    16021 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
    16081 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
    16141 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
    16201 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
    16261 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
    16321 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
    16381 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc
    16441 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
    16501 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
    16561 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
    16621 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
    16681 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
    16741 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
    16801 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
    16861 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
    16921 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
    16981 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
    17041 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
    17101 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
    17161 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
    17221 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
    17281 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
    17341 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
    17401 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
    17461 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
    17521 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
    17581 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg
    17641 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
    17701 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
    17761 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
    17821 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
    17881 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
    17941 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
    18001 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
    18061 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
    18121 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
    18181 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
    18241 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
    18301 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
    18361 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
    18421 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
    18481 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
    18541 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
    18601 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
    18661 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
    18721 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
    18781 caaattgccc tagcagggga aaaaggtcga aaaggactct ttcctgtgga tagcacgtac
    18841 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
    18901 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
    18961 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
    19021 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
    19081 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
    19141 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
    19201 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
    19261 acacatgcag ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    19321 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
    19381 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
    19441 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    19501 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    19561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    19621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    19681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    19741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    19801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    19861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    19921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    19981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    20041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    20101 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    20161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    20221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    20281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    20341 attttggtca tgcattctag gtgattagaa aaactcatcg agcatcaaat gaaactgcaa
    20401 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
    20461 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
    20521 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
    20581 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
    20641 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
    20701 caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa
    20761 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
    20821 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat
    20881 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
    20941 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
    21001 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta
    21061 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
    21121 atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat
    21181 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    21241 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    21301 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    21361 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    21421 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    21481 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    21541 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    21601 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattcgt ctca
//
