LOCUS       sequence-460243-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a pH sensitive variant of EGFP inserted in the 
            C-peptide of insulin for pH measurement in insulin secretory 
            granules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   De Lorenzi V, Ghignoli S, Bernardi M, Matteoli G, Ferri G, Storti B,
            Bizzarri R, Cardarelli F
  TITLE     Measuring pH in insulin secretory granules by phasor-based 
            fluorescence lifetime imaging of a genetically encoded sensor.
  JOURNAL   Commun Biol. 2025 Feb 25;8(1):304. doi: 10.1038/s42003-025-07758-w.
  PUBMED    40000885
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-02-25"; volume: "8(1):304. doi"; pages: " 
            10.1038/s42003-025-07758-w"
COMMENT     Sequence Label: pCpep-E1GFP
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(125..144)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             284..535
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     472..493
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtgaccacc ctgagctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
       61 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      121 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      181 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      241 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      301 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      361 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      421 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      481 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
      541 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggagc
      601 aggtgacctt cagaccttgg cactggaggt ggcccagcag aagcgtggca ttgtagatca
      661 gtgctgcacc agcatctgct ccctctacca gctggagaac tactgcaact ag
//
