LOCUS       sequence-460262-        2844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal integrating shuttle vector depleted of restriction sites 
            outside the polylinker region, yeast selection marker URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Scutteri L, Barth P, Rahi SJ
  TITLE     Minimal integrating shuttle vectors for Saccharomyces cerevisiae 
            depleted of restriction sites outside the polylinker region
  JOURNAL   bioRxiv 2024.11.05.622133
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.05.622133"
COMMENT     Sequence Label: pLS406
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(283..302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1437..1456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1537..1553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1537..1553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1610..1626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1622..1842
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttactag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaacagg aaacagctat gacgcgcgcg
     1561 agctcggtac ccgggatcct ctagagtcga cctgcaggca tgccctagga ctggccgtcg
     1621 ttttacttca attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat
     1681 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
     1741 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
     1801 caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat
     1861 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
     1921 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccacgaagga attactagag
     1981 ttagttgaag cattaggtcc gaaaatttgt ttactaaaaa ctcatgtgga tattttgact
     2041 gatttttcga tggaggggac agttaagccg ctaaaggcat tatcagccaa gtataatttt
     2101 ttactcttcg aggacagaaa atttgctgac attggtaata cagtcaaatt gcagtattct
     2161 gcgggtgtat atagaatagc agaatgggca gacattacga acgcacacgg tgtggtgggg
     2221 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggg
     2281 cttttgatgt tagcagaatt gtcatgcaag ggttccctaa gcactggtga atatactaag
     2341 ggtactgttg atattgccaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
     2401 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     2461 gatgacaagg gtgacgcatt gggccaacag tatagaaccg tagatgatgt agtctctaca
     2521 ggatcagaca ttattattgt aggaagagga ctatttgcaa agggaaggga tgcaaaggta
     2581 gagggtgagc gttacagaaa agcagggtgg gaagcatatt tgagaagatg cgggcagcaa
     2641 aactaaaaaa ctgtattata agtaaatgaa tgtataataa actcacaaat tagagcttca
     2701 atttaattat atcagttatt acccggaaat cttggtcgta atgatttcta taatgacgaa
     2761 aaaaaaaaaa ttggaaagaa aaaacttcat ggcctttata aaaaggaact atccaatacc
     2821 tcgccagaac caagtaacag tatt
//
