LOCUS       sequence-460268-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Gephyrin at the his-3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  TITLE     Convergent Evolution Links Molybdenum Insertase Domains with 
            Organism-Specific Sequences.
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCCG_Gephyrin
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4979..4995
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4979..4995
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7284..7300)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7285..7301)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(join(9999..10000,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(10000,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgaggccta gcggtcccag ctccccgtaa
     2761 ccacgcagcc ccagcgaccg aggtcatcat gacactatga cgacaatctg gcgggagact
     2821 tgggaaaatc gatgacgggc acctaccttg ggaacaatag agcaagtcca gtcaacgccc
     2881 tagggaaggg ggaaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat acgactcact
     4021 atagggcgaa ttggagctcc accgcggtgg cggccgctag aaggagcagt ccatctgcgt
     4081 gaatcacgag agaatcagct actttgaatc gatggatgca gctaccagaa gtcactcagt
     4141 tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg gacgagaccg
     4201 acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg cggccttcgg
     4261 taacagaaaa gcacacagac agcgattgtg acatggattc gggcaaagca ttggtggtcg
     4321 caccaggtca tcctgagtgt gcagtggctg ctattcagat ttcatctaac tgcgggagag
     4381 gggttcaaag gggcgtgacg tcacagacaa cgggtgaagg acgaagattg cctcacttct
     4441 ttgctagcaa tttgcctgca aagaaggcgc acatgtcaag caaacaactg ggaaaccact
     4501 attgaatacc cacaatgcaa agctcggaag gtacgtcttg attgcagtgt gtcgagtgtc
     4561 aaaaaagaag caagtgttca tgcaagccaa aattggcacc tcctccactt ctccgagtgc
     4621 cccacccgaa cctccaggcg agatggccgg aacataccat ccgcgttggg attatgacgt
     4681 atctccttct tcttcacatg attccatccc gttgttgctt gtttgcgagc tgtgacggga
     4741 gatcgtagat gccacttagg gccaggcagg cagtgcaggc agccaggaac acaagcttcc
     4801 aacttggtca tctcgattgc cgattaaggg aaccaaatgc ctatataaga ctgtcctccc
     4861 acctccccaa taccattctt ttcttcttcc atcatcagcc aacaaagcaa tcacatcttc
     4921 actacttcaa atcaacacaa cactcaaacc actttcacaa cccctcacat caaccaaatc
     4981 tagaactagt ggatccccgg gttaattaac atggcgaccg agggaatgat cctcacaaac
     5041 cacgaccatc aaatccgtgt cggagtcctc acagtgagtg atagctgctt caggaatctt
     5101 gcagaagacc gcagtgggat aaatctaaaa gatctcgtcc aggatccttc tttgttgggt
     5161 gggactatat cagcatacaa gatagtacca gatgaaatag aagaaatcaa ggaaacactc
     5221 atagactggt gtgatgagaa ggaacttaat ttgatattaa caactggcgg aacagggttt
     5281 gcaccacgag atgtcactcc agaggccaca aaagaagtaa tagaacggga agcgccaggg
     5341 atggccctgg caatgctgat gggatcactt aatgttacac ctctgggcat gctctctaga
     5401 ccagtgtgtg gaataagagg gaaaactctg ataattaacc tgcctggtag caagaaagga
     5461 tctcaggaat gctttcagtt catactgcca gctctacctc atgccattga ccttttacgt
     5521 gatgccattg taaaagtaaa ggaggtgcat gatgaacttg aagatttacc ttccccacca
     5581 cctcctcttt ctccacctcc tacaactagc ccacataagc agacagaaga caaaggagtt
     5641 cagtgtgaag aggaggaaga agaaaagaaa gacagtggtg tagcttcgac agaagatagt
     5701 tcctcatcac atattactgc agcagctatt gctgcaaaga ttccagactc catcatttct
     5761 cgtggcgttc aggtgctccc acgagacaca gcctccctta gcactactcc ttcagagtcg
     5821 ccccgtgccc aggctacatc tcgcctctct acagcttctt gtccaacacc aaaagctcgg
     5881 cttccctcgt gctcacctac ctatagtgta tctgaggtcc agtccaggtg cagcagcaag
     5941 gagaacattc taagagccag tcacagtgct gtagatatca ccaaggtggc tagaagacat
     6001 cgcatgtctc cttttcctct gacgtctatg gacaaagcct tcattacagt tctggagatg
     6061 actccggtgc ttggtacaga aatcatcaat taccgagatg gaatggggcg agtccttgct
     6121 caagatgtgt atgcaaagga caacctaccc ccattcccag catcagtaaa agatggctat
     6181 gctgttcgag ctgctgatgg tccaggagat cgtttcatca ttggggaatc ccaagctggt
     6241 gaacagccaa ctcagacagt aatgcccgga caagtgatgc gggttacaac aggtgctcca
     6301 atcccctgcg gtgctgatgc agtagtgcaa gttgaggata ctgaactcat cagggaatca
     6361 gatgatggta ctgaagaact tgaagtgcga attctggtgc aagctcggcc aggccaagac
     6421 atcagaccca tcggccacga cattaaaaga ggggagtgtg ttttggcaaa aggaacccat
     6481 atgggccctt cagaaattgg tctccttgca actgtaggtg tcacagaagt tgaagtcaat
     6541 aagtttccag tggttgccgt tatgtcaaca gggaatgagc tactaaatcc tgaagatgac
     6601 ctcttaccag gaaagattcg ggacagcaat cgatcaacac ttctagcaac aattcaggaa
     6661 catggttacc cgacaatcaa cttgggaatt gtaggagaca acccagatga cttactcaat
     6721 gccttgaatg agggtatcag ccgtgctgat gtcatcatca cgtcaggagg tgtatccatg
     6781 ggggaaaagg actatctcaa gcaggtgctg gacatcgatc ttcatgctca gatccatttt
     6841 ggcagggttt ttatgaagcc aggcttgcca acaacatttg caactttgga tattgatggt
     6901 gtaagaaaaa ttatttttgc actaccaggg aatcctgtat cagctgtggt cacctgcaac
     6961 ctctttgttg tacccgcact gagaaaaatg cagggtatct tggatcctcg gccaaccatc
     7021 atcaaagcta ggttatcttg tgacgtaaag ctggaccctc gcccagaata ccaccggtgt
     7081 atactgactt ggcatcacca agaaccactg ccctgggccc agagtacagg taatcagatg
     7141 agcagccgtc tgatgagcat gcgcagtgcc aatggattgc tgatgctacc tccaaagaca
     7201 gagcagtatg tggaacttca caaaggcgag gtggtggacg tcatggtcat tggacggcta
     7261 tgagaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtaccagc
     7321 ttttgttccc tttagtgagg gttaataggg cgtgcacggc tatggggtct gaatgctaaa
     7381 agacaccatt tcccacactc cctctgattt tttttatggt ttttttttat cttcattttt
     7441 cgccgtcttt tattcggttg tttgggaggt tcacatgcaa cgtgcaagac tgcaagtata
     7501 ttgagatggg cgcttcatgc aatgcagcat cactgcagcg acattgtgac atccacgttt
     7561 cgatcgtctg tgcttccccg ttcatgtacg catgatctga aaaaaaaaaa aaaaaaaaag
     7621 ttgatgtaat aatgaaaata aacacccagg gacgagcacc ccttctggaa tagggagaaa
     7681 aactaaaggg acgtttgggt ctctacactt accaacagat tcatgtactg atcttcagcc
     7741 tgtacagaac agtacaatgt ccaaaactcg ttatcattac tctatgtgcc caaaatgacc
     7801 cagatataca tcaacccact acatcctttt gttcttgttt tttttttttt tttgctactt
     7861 cgaaaagaat ttccgtttag ttgctcttgc tcatgtgctc aagacggatg ctgaccgccc
     7921 ttctgtgagc ctcgagctcc tcaaccttag ccaactgcat gacagcctgg ccgacgtttt
     7981 tgagaccctc ggcagtcaag ttggagctgg taatgtgctt gacgaacgag gcgagattga
     8041 cgccagagta ctgcttggca aagccatagg tagctatgtg tcttgacgtt agcagaagaa
     8101 tctgtgaagc ggggctattt atgatatgta acttacgcag cgagtggtta acaccagcag
     8161 agtaatcgcc aacggactca ggagtccagg cgccaatgaa gacactaccg gcgttcatga
     8221 caagatcgac agccttctcg gcctccttga tctggaggat caagtgctca ggagcgtact
     8281 tgttgctgag ctccatggcc tcctcgacgg tcttgacctg cacggtgatc gagtgggcga
     8341 tggagccacg gacaatctgg acgcgaggaa gctccgtagc ctgacggtga acctcgtcct
     8401 caatagcctg aagatgctcc tcgtcgaggt caatagcgat caggatgacc tgactgtcaa
     8461 cgccgtgctc agcctgggac aggagatccg aggcaacgaa cgcggggttg gcgtccttgt
     8521 cagcgatgac cagcacctcg gacgggccag cgggcatgtc aataccaacg gcagcgttgg
     8581 tgtcgttgct gacgaacatc ttggcagcag tgacgaactg gttaccgggg ccgagaatct
     8641 tgtcgacctt ggtgatgctc tcggtgccgt aggccatggc agctacggcc tgggcaccgc
     8701 cggcaagcac gatggactcg gcgccaacct tgtgagcgac gtagacaatc tcgggagtga
     8761 tggttccgtc ggcgcgggga ggagaggcga acacaatctt gttgcagccg gcgaccatgg
     8821 cgggaacacc cagcataagg gcagtgctgg ggagaacggc ggtaccgccg gggatgtagc
     8881 agccgacggc ctcgatggga cgagagaaac ggctgcagac aacaccgggc atggtctcga
     8941 cctggagggg cttctcctcc ttctgggcgg cgtggaactt gcggatgttc tcgaaggaca
     9001 cgtcgatggc agcaatggtc tcctcaggga gctgcataag ctccttgggg aagggcgcct
     9061 tcaggacggg gctagtaaga gaggtagcct tctcgaactt gtgagtgtac gacagaacag
     9121 ccttgtcgcc gttcttgcgg acgtcctcga tgatggggac aatgatcttg tagatggcat
     9181 cggacgactt ttgcgcagga cgcttgagag cagcatcgag ctcctcggta gagaccttgg
     9241 aggcgtcgaa acgtctcatg gtgatcttct caggggtggt ctcctgggcg gcctccttgg
     9301 tgacaggggc cgaagtggca gcgagagcgg acgccgcagg cttgatgccc tccttctcag
     9361 cccacttacc cttagcatct ccagtcctgc gcttgacctt ccagctcttg gcgtcaaggc
     9421 tcctttcgat atcggcaaga gtaacgccgg cagcaacggc cctggtaaga gcaaagtaga
     9481 agagatcggc agcctcaaag gcgatttcct ggggggtctg agcggtgcag agctcctcag
     9541 cctcctccat gatcttggcc cggactagct tctcatcgga gaagagacgg gcagtgtagg
     9601 agccctcggg ggcagactgt ttcctcgaaa tcaaagtctg ctcgagcttg ggaaggcctt
     9661 tgagctgacc aaagcagccg gactgatcga ggtggcagaa acgacccttc tgcttcacga
     9721 caaacttgag agcatcgtta tcgcagtcaa gcgagatgcg gacgagctcc tgagtgtctc
     9781 cggaagtagc acccttgtac cagagaccgc gcttccggct ctgatagaca ccagtctgtg
     9841 tcctgagggc ctcgttcaca ctctcggcac tgctgtagac cagacccaga gcagtgtcgt
     9901 gctcatcaac gacaacggtg gggagaagac catcgggacg gtcagacttc cagacgctcg
     9961 agaggatggt ggagatggca agcttcagct gctcgagttc
//
