LOCUS       sequence-460272-        8710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expressing membrane-localized Voltron2 and membrane-localized 
            CheRiff..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Wong-Campos JD, Park P, Davis H, Qi Y, Tian H, Itkis DG, Kim D, 
            Grimm JB, Plutkis SE, Lavis L, Cohen AE
  TITLE     Voltage dynamics of dendritic integration and back-propagation in 
            vivo.
  JOURNAL   bioRxiv [Preprint]. 2023 May 26:2023.05.25.542363. doi: 
            10.1101/2023.05.25.542363.
  PUBMED    37292691
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 May 26:2023.05.25.542363. doi: 
            10.1101/2023.05.25.542363."
COMMENT     Sequence Label: PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eG...
FEATURES             Location/Qualifiers
     source          1..8710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   38..167
                     /label=AAV2 ITR
     promoter        481..758
                     /label=chicken beta-actin promoter
     primer_bind     1037..1059
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1121..1140
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1191..1224)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1275..1308)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(1515..2225,2226..2228))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1515..2225,2226..2228))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1557..1578)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1906..1925
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2165..2186
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2244..3188)
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="GGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQC
                     SSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVI
                     LEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLV
                     SDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLVL
                     AMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIH
                     EHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             complement(3189..3245)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4926..4964)
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     regulatory      5027..5036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5043..5076
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5127..5160
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5185..5773
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5238..5258)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5656..5667)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5776..5792)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5777..5793)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5822..5943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5833..5852)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5887..5906
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6105..6234
                     /label=AAV2 ITR
     rep_origin      complement(6471..7059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6551..6570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7230..8021,8022..8090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7853..7872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8091..8195)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8221..8676)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8358..8379)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8570..8589
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agggcgatcg gtgcgggcct cttcgctatt acgccagctg cgcgctcgct cgctcactga
       61 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
      121 gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt agttaatgat
      181 taacccgcca tgctacttat ctacgtagcc atgctctagg aagatctgta ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      541 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      601 ggcgcgcgcc rggsggggsg gggcggggcg aggggcgggg cggggcgagg cggagaggtg
      661 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
      721 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
      781 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      841 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      901 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
      961 gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg
     1021 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1081 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1141 ctgtctcatc attttggcaa agaattggat cctctagagt cgactccgga ataacttcgt
     1201 ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg ctattagcaa
     1261 tatgaaacct cttaataact tcgtatagca tacattatac gaagttatgg cgcgccttag
     1321 aatctggtgg cttcagggaa gtccgtggca cagctgatga agctgtcaat gctggacatg
     1381 ctccgcatgt cgatgacgcc ctccgtgcgt gcgggcagag gggccacggg gctgggcatg
     1441 ctgctcatct ccagctcctc tggaggcttg ctctgcgggg ccatctcctt ggtgttgagg
     1501 atgggctggg actgcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     1561 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     1621 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     1681 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     1741 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     1801 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     1861 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     1921 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     1981 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     2041 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcaaat gaacttcagg
     2101 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     2161 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     2221 ttgctcacgc tcttggcggc cgccacttta tcttcctctg tcacgaaggc cagcacgtcc
     2281 accttgcgtc ccagaaactg ggagctcact ggtctcctga tatcgccgta cataatgata
     2341 tgctcgtgaa ttttgatccg caggaagtgc cccagcagtc cccaaatatt cttggacagc
     2401 aggtcggcga tggtatggcc aatagtggac ccagccacgc tcagggtgtg catgccttca
     2461 ggcccaaaga tgaacaggcc gggaaacatc agccaactag aatagtacac ggcagtcatg
     2521 gccagcacca gtttcttaca gccgccggca ggcatgatat agtaagactc cacgtagatg
     2581 attccagcgt tgtagaatgt ctgggttcca tacaccaggc cgatacagta aaacagccac
     2641 ttcacccacc cagtggccag agcggctgtc actcccatgc aaatagttcc caggtcggac
     2701 accagcagag ccattgtccg cttattgtat gcctcactca ggccggtgat gttagacaga
     2761 tgaatcagga tcacgggaca tgtcagcagc cattcggcat atctcagcca tggggtaatg
     2821 ttccctccgt acaggaacag catagcagga ctggtgaact cgaaatagat ctccagaatc
     2881 actttgatca gctccacgga gcacacgtac acttcctccc atcccacgct ggccttatag
     2941 gcgtgccagc cgtagaaagc cagaaacagg gcagacagga acacggcaaa ccactggaag
     3001 cccctagctg tttcctgctc ccacagggcc ccatagtaga tgtagatgga gctgcactgt
     3061 tcctcatacc catgcacggg gtggtagggt ggattcactt gatatccagc tggggcatgg
     3121 tactcactgc ctccggcaga atcgtttcca ggtggggcgc tgtgagcgtc tggagcagga
     3181 gctccgccgg gaccggggtt ttcttccacg tctcctgctt gctttaacag agagaagttc
     3241 gtggctccgg agcccacctc gttctcgtag cagaacacgt tgatgtcgat ctggtccagg
     3301 gggatgtact cgccctcgct ggtgatcctg ctcttagtgg ttggctcgcc ggaaatctcg
     3361 agcgtcgaca gccagcgcgc gatctcgctg ccgatcaggt ccgggttgtc ttcttgcagc
     3421 agattcagac ccgggccgat gtccacagcc ttgcagttag gcaggctttt ggccaggcga
     3481 gcggcttcgg ccggtgggat cagaacgcct ggggtgcccc agaacagcag cttcgggaca
     3541 ggggactggt gcagccagtc catgtattct tcgaccagcg cgacgatgtt cgctggctca
     3601 ccggcgattg gcagctcgtt tgggaagcgc cacagtggct cgcggtcaac aggattcagg
     3661 aacggctcgc ggtaatggtc catctcgact tcagtcagcg ggcggacgac acccatcggc
     3721 agcgtaccct cgataaaaac gttctgatcg atgatcagct tgcggccgac gtcggtggtg
     3781 cggaaggcct ggaaggtctc gcgggcaaat tctggccatt cgtcccaggt cgggataggg
     3841 cggatgaact ccataaatgc aatacctttg acgcgctctg gattgcgctt ggcccagtgg
     3901 aaacccagag cggagcccca gtcgtgaatg accaggacga cctcttccag acccagggct
     3961 tcgatgaagg catccatgaa gcggacgtgg tcgtcgaaga aataacccag gtctggtttg
     4021 tcggatttgc ccataccgat caggtctgga gcaatgcagc gatgggtcgg tgcaacatgc
     4081 gggatgatgt tgcgccacac gtaggaggag gtcgggttac cgtgcaggaa cagcacaggg
     4141 gtgccatcgc gcggaccaac atcgacgtag tgcatgcgct cgcccaggac ttccacataa
     4201 tgggggtcga atggaaagcc agtaccgatc ttgaagatag tctcatgggc aatgaggcac
     4261 cccattccaa acagtggctt agcgagcaca tccaggattc ccatgagaat ggcctcattg
     4321 tcgacggaca gcacgccact accagacccg aaagcccaca cgacaggata aaacacccag
     4381 aggattgaca ggaagacgag cagactgaca taggagctat ggtactcggg gcttttcttc
     4441 agatcgtctt tccaagtggc gttatacatc ccgtatgtca ggactgcctg catcacacat
     4501 ccagcgataa agtacaccca tttgaacttg tgttcatcct cgaagtcgcc cagaatacca
     4561 aacacgatca tgaaaatgtc tgcgccgatg acccaagaaa tcatcactcc gcccatcttg
     4621 gtcatgacga tgagatcgag caggagcagt ggtgtggtca gcacccagtt aatatatctt
     4681 gcgtagacca cctgcctttc accattcatt gtcaggtcct ggtagttcac ggccattgca
     4741 tagtaagcgg tagcggcgat agtgcagatg gcaatgttga tatagtatgc gctcttcagc
     4801 tctgaaggcc gaaagtgcac agccactcca aaagcaatag cagcagcagc cataatagca
     4861 aagacaatcc actgtgcaat catgccggtc tcggtttcca cgtcagcact accggatctg
     4921 actacgcctg tcttattgct gaaaggaacg tagaagttgg gaccttctgt tccgttagcc
     4981 agtccaaggg tcaacaaagc cagcaagagg agtatttggg gtcggcccat ggtggcgcta
     5041 gcataacttc gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac
     5101 tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat acgaagttat
     5161 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     5221 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5281 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5341 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5401 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5461 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5521 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5581 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5641 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5701 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5761 gccgcctccc cgcatcgata ccgtcgacct cgacccgggc ggccgcttcg agcagacatg
     5821 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5881 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5941 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     6001 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca
     6061 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat
     6121 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     6181 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat
     6241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8221 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     8281 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     8341 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     8401 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     8461 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8521 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     8581 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8641 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     8701 actgttggga
//
