LOCUS       sequence-460281-        9741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac vector to express MLV Gag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pPB_EF1a_Gag
FEATURES             Location/Qualifiers
     source          1..9741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   127..189
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1356..2534
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1587..2525
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2482..2502
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2541..2550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2550..2966
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2896..2918
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2934..2950
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4146..4202
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      4455..4464
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4926..5514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4979..4999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5397..5408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5564..5685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5601..5620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5655..5674
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6448..6482
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6973..6989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6973..6989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6986..7008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6997..7013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7021..7027,7028..7045,7046..7051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7066..7087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7204..7221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7375..7963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7455..7474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8134..8925,8926..8994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8757..8776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8995..9099)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9125..9580)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9262..9283)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9474..9493
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9707..9729
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9721..9738
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9722..9738
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
       61 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
      121 gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgtgtaaaat
      181 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
      241 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
      301 tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
      361 ttatgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
      421 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
      481 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
      541 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc
      601 tccaccataa agatggttaa ttaacccacc caagatctgg atcgataagc ttgatatcga
      661 attcgggagg tggtccctgc agttacgcca atgataaccc ccgccagaaa aatcttagta
      721 gccttccctt tttgttttcc gtgccccaac tcggcggatt gactcggccc cttccggaaa
      781 cacccgaatc aacttctagt caaattattg ttcacgccgc aatgacccac ccctggcccg
      841 cgtctgtgga actgacccct ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg
      901 ggtactagtt tttaagctcc caactccccc tcccccagcg tctggaggat tccacaccct
      961 cgcaccgcag gggcgaggaa gtgggcggag tccggttttg gcgccagccg ctgaggctgc
     1021 caagcagaaa agccaccgct gaggagactc cggtcactgt cctcgccccg cctccccctt
     1081 ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc
     1141 gcctccgccc tccccctagg gccccaattc ccagcgggcg cggcgcgcgg ccccgcgcgc
     1201 agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga gtcctgcagc
     1261 cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1321 ctgtaggttt ggcaatcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     1381 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg
     1441 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     1501 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     1561 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     1621 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     1681 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1741 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1801 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1861 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1921 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1981 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     2041 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     2101 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     2161 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     2221 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     2281 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     2341 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     2401 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     2461 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     2521 ttcaggtgtc gtgacgtacg gccaccatgg gccagactgt taccactccc ttaagtttga
     2581 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     2641 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     2701 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     2761 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     2821 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     2881 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     2941 cagccctcac tccttctcta ggcgccaaac ctaaacctca agttctttct gacagtgggg
     3001 ggccgctcat cgacctactt acagaagacc ccccgcctta tagggaccca agaccacccc
     3061 cttccgacag ggacggaaat ggtggagaag cgacccctgc gggagaggca ccggacccct
     3121 ccccaatggc atctcgccta cgtgggagac gggagccccc tgtggccgac tccactacct
     3181 cgcaggcatt ccccctccgc gcaggaggaa acggacagct tcaatactgg ccgttctcct
     3241 cttctgacct ttacaactgg aaaaataata acccttcttt ttctgaagat ccaggtaaac
     3301 tgacagctct gatcgagtct gtcctcatca cccatcagcc cacctgggac gactgtcagc
     3361 agctgttggg gactctgctg accggagaag aaaaacaacg ggtgctctta gaggctagaa
     3421 aggcggtgcg gggcgatgat gggcgcccca ctcaactgcc caatgaagtc gatgccgctt
     3481 ttcccctcga gcgcccagac tgggattaca ccacccaggc aggtaggaac cacctagtcc
     3541 actatcgcca gttgctccta gcgggtctcc aaaacgcggg cagaagcccc accaatttgg
     3601 ccaaggtaaa aggaataaca caagggccca atgagtctcc ctcggccttc ctagagagac
     3661 ttaaggaagc ctatcgcagg tacactcctt atgaccctga ggacccaggg caagaaacta
     3721 atgtgtctat gtctttcatt tggcagtctg ccccagacat tgggagaaag ttagagaggt
     3781 tagaagattt aaaaaacaag acgcttggag atttggttag agaggcagaa aagatcttta
     3841 ataaacgaga aaccccggaa gaaagagagg aacgtatcag gagagaaaca gaggaaaaag
     3901 aagaacgccg taggacagag gatgagcaga aagagaaaga aagagatcgt aggagacata
     3961 gagagatgag caagctattg gccactgtcg ttagtggaca gaaacaggat agacagggag
     4021 gagaacgaag gaggtcccaa ctcgatcgcg accagtgtgc ctactgcaaa gaaaaggggc
     4081 actgggctaa agattgtccc aagaaaccac gaggacctcg gggaccaaga ccgcagggat
     4141 ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     4201 cgatggtcag caagggcgag gaggacaacg cctctctccc agcgacacac gagttacaca
     4261 tctttggctc catcaacggt gtggacttcg acctcgtggg tcagggcacc ggcaatccaa
     4321 acgacggtta cgaggagctc aacctcaagt ccaccaaggg cgacctccag ttctccccct
     4381 ggattctggt ccctcatatc gggtacggct tccatcagta cctgccctac ccagacggga
     4441 tgtcgccttt ccaggccgcc atggtggacg gctccggata ccaagtccat cgcacaatgc
     4501 agttcgaaga cggtgcctcc cttactgtca actaccgcta cacctacgag ggaagccaca
     4561 tcaaaggaga ggcccaggtc aaggggactg gtttccctgc cgacggtcct gtcctcacca
     4621 actcgctcac cgctgcggac tggtgcaggt cgaagaagac ttaccccaac gacaaaacca
     4681 tcatcagtac cttcaagtgg agttacacca ctggaaacgg caagcgctac cggagcactg
     4741 cgcggaccac ctacaccttt gccaagccaa tggcggccaa ctatctcaag aaccagccga
     4801 tgtacgtgtt ccgcaagacg gagctcaagc actccaagac cgagctcaac ttcaaggagt
     4861 ggcaaaaggc ctttaccgac gtcatgggca tggacgagct ctacaagtaa acgcgttaag
     4921 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4981 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5041 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5101 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5161 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5221 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5281 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5341 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5401 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5461 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctcgag
     5521 tctagagggc ccgtttaaac ccgctgatca gcctaactaa ctaaacttgt ttattgcagc
     5581 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5641 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg
     5701 actcaaatga tgtcaattag tctatcagaa gctcatctgg tctcccttcc gggggacaag
     5761 acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct
     5821 ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag
     5881 tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg
     5941 ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
     6001 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
     6061 gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta
     6121 gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct
     6181 ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat
     6241 aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac
     6301 tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac
     6361 gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta
     6421 gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag
     6481 ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc
     6541 caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg
     6601 aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac
     6661 gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt
     6721 tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg
     6781 tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg
     6841 gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc
     6901 ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt
     6961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9121 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     9181 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     9241 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     9301 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     9361 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     9421 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     9481 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     9541 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     9601 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     9661 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     9721 tgtaaaacga cggccagtga g
//
