LOCUS       sequence-460282-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express an MLV Gag-EIF4E fusion protein (W56L, 
            W102L, W166L mutations installed into EIF4E).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CAG_Gag-EIF4E-TM
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(223..1014,1015..1083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     846..865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1084..1188)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1263..1282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1454..1833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1835..2033
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2051..2231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2278..2403
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2896..3129
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3314..3358
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3656..3773
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3808..4186
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4187..4390
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4387..4411
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4524..4999
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      5054..5063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5063..5479
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5409..5431
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5447..5463
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7340..7396
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7397..7768
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    7793..8381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7846..7866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8264..8275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8384..8400)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8385..8401)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8906..9086
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9112..9129)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9118..9342
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9388..9816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9475..9494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9685..9706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9842..9858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9842..9858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9855..9877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9866..9882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9890..9896,9897..9914,9915..9920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9935..9956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10073..10090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10244..10780,1..52))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10324..10343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
       61 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1141 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1201 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     1261 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     1321 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     1381 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     1441 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     1501 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     1561 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1621 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     1681 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     1741 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     1801 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     1861 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     1921 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     1981 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     2041 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     2221 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     2281 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     2341 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     2401 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     2461 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     2521 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     2581 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     2641 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     2701 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     2761 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     2821 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     2881 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     2941 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3001 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3061 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     3121 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     3181 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     3241 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     3301 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     3361 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     3421 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     3481 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     3541 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     3601 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     3661 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3721 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     3781 attacaggga cagcagagat ccagtttaca ttgattattg actagttatt aatagtaatc
     3841 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3901 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3961 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4021 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4081 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4141 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     4201 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4261 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4321 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4381 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     4441 cctccataga agacaccggg accgatccag cctcccctcg aagcttacat gtggtaccga
     4501 gctcggatcc tgagaacttc agggtgagtc tatgggaccc ttgatgtttt ctttcccctt
     4561 cttttctatg gttaagttca tgtcatagga aggggagaag taacagggta cacatattga
     4621 ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact
     4681 tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat
     4741 acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa
     4801 ggcaatagca atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga
     4861 ggtttcatat tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt
     4921 gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc
     4981 tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt
     5041 tggcaaagaa ttcgccacca tgggccagac tgttaccact cccttaagtt tgaccttagg
     5101 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     5161 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     5221 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     5281 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     5341 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     5401 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     5461 cactccttct ctaggcgcca aacctaaacc tcaagttctt tctgacagtg gggggccgct
     5521 catcgaccta cttacagaag accccccgcc ttatagggac ccaagaccac ccccttccga
     5581 cagggacgga aatggtggag aagcgacccc tgcgggagag gcaccggacc cctccccaat
     5641 ggcatctcgc ctacgtggga gacgggagcc ccctgtggcc gactccacta cctcgcaggc
     5701 attccccctc cgcgcaggag gaaacggaca gcttcaatac tggccgttct cctcttctga
     5761 cctttacaac tggaaaaata ataacccttc tttttctgaa gatccaggta aactgacagc
     5821 tctgatcgag tctgtcctca tcacccatca gcccacctgg gacgactgtc agcagctgtt
     5881 ggggactctg ctgaccggag aagaaaaaca acgggtgctc ttagaggcta gaaaggcggt
     5941 gcggggcgat gatgggcgcc ccactcaact gcccaatgaa gtcgatgccg cttttcccct
     6001 cgagcgccca gactgggatt acaccaccca ggcaggtagg aaccacctag tccactatcg
     6061 ccagttgctc ctagcgggtc tccaaaacgc gggcagaagc cccaccaatt tggccaaggt
     6121 aaaaggaata acacaagggc ccaatgagtc tccctcggcc ttcctagaga gacttaagga
     6181 agcctatcgc aggtacactc cttatgaccc tgaggaccca gggcaagaaa ctaatgtgtc
     6241 tatgtctttc atttggcagt ctgccccaga cattgggaga aagttagaga ggttagaaga
     6301 tttaaaaaac aagacgcttg gagatttggt tagagaggca gaaaagatct ttaataaacg
     6361 agaaaccccg gaagaaagag aggaacgtat caggagagaa acagaggaaa aagaagaacg
     6421 ccgtaggaca gaggatgagc agaaagagaa agaaagagat cgtaggagac atagagagat
     6481 gagcaagcta ttggccactg tcgttagtgg acagaaacag gatagacagg gaggagaacg
     6541 aaggaggtcc caactcgatc gcgaccagtg tgcctactgc aaagaaaagg ggcactgggc
     6601 taaagattgt cccaagaaac cacgaggacc tcggggacca agaccgcagg gatcaggatc
     6661 aggatcagga tcaggatcta tggcgactgt cgaaccggaa accaccccta ctcctaatcc
     6721 cccgactaca gaagaggaga aaacggaatc taatcaggag gttgctaacc cagaacacta
     6781 tattaaacat cccctacaga acagatgggc actctggttt tttaaaaatg ataaaagcaa
     6841 aactttgcaa gcaaacctgc ggctgatctc caagtttgat actgttgaag acttttgggc
     6901 tctgtacaac catatccagt tgtctagtaa tttaatgcct ggctgtgact actcactttt
     6961 taaggatggt attgagccta tgttggaaga tgagaaaaac aaacggggag gacgatggct
     7021 aattacattg aacaaacagc agagacgaag tgacctcgat cgcttttggc tagagacact
     7081 tctgtgcctt attggagaat cttttgatga ctacagtgat gatgtatgtg gcgctgttgt
     7141 taatgttaga gctaaaggtg ataagatagc aatattgact actgaatgtg aaaacagaga
     7201 agctgttaca catataggga gggtatacaa ggaaaggtta ggacttcctc caaagatagt
     7261 gattggttat cagtcccacg cagacacagc tactaagagc ggctccacca ctaaaaatag
     7321 gtttgttgtt ggatctggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     7381 agagaatcct gggccggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     7441 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     7501 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     7561 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     7621 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     7681 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     7741 gcacttcgtg gccgaggagc aggactgaga attcgatatc aagcttatcg gtaatcaacc
     7801 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7861 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7921 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7981 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8041 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8161 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     8221 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8281 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8341 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     8401 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     8461 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     8521 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8581 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     8641 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     8701 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     8761 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     8821 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     8881 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     8941 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9001 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9061 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     9121 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9181 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9241 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9301 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9361 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     9421 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9481 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9541 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9601 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9661 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9721 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9781 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtagcttg gcgtaatcat
     9841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
//
