LOCUS       sequence-460289-       11541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector to express an MLV Gag-EIF4E fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, 
            Blainey PC
  TITLE     Live-cell transcriptomics with engineered virus-like particles
  JOURNAL   bioRxiv 2024.10.01.616098
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.01.616098"
COMMENT     Sequence Label: pLV_CAG_Gag-EIF4E
FEATURES             Location/Qualifiers
     source          1..11541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(223..1014,1015..1083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     846..865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1084..1188)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1263..1282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1454..1833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1835..2033
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2051..2231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2278..2403
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2896..3129
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3314..3358
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3656..3773
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3808..4186
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4187..4389
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4386..4410
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4523..4998
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      5053..5062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5062..5478
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5408..5430
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5446..5462
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7339..7395
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7396..8091
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR
                     TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF
                     KTPDADAGEE"
     CDS             8101..8157
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8158..8529
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     misc_feature    8554..9142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8607..8627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9025..9036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9145..9161)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9146..9162)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9667..9847
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9873..9890)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9879..10103
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10149..10577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10236..10255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10446..10467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10603..10619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10603..10619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10616..10638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10627..10643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10651..10657,10658..10675,10676..10681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10696..10717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10834..10851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11005..11541,1..52))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11085..11104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
       61 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1141 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1201 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     1261 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     1321 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     1381 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     1441 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     1501 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     1561 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1621 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     1681 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     1741 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     1801 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     1861 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     1921 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     1981 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     2041 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     2221 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     2281 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     2341 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     2401 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     2461 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     2521 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     2581 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     2641 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     2701 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     2761 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     2821 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     2881 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     2941 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3001 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3061 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     3121 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     3181 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     3241 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     3301 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     3361 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     3421 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     3481 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     3541 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     3601 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     3661 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3721 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     3781 attacaggga cagcagagat ccagtttaca ttgattattg actagttatt aatagtaatc
     3841 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3901 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3961 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4021 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4081 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4141 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggtttgg
     4201 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     4261 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     4321 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     4381 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     4441 ctccatagaa gacaccggga ccgatccagc ctcccctcga agcttacatg tggtaccgag
     4501 ctcggatcct gagaacttca gggtgagtct atgggaccct tgatgttttc tttccccttc
     4561 ttttctatgg ttaagttcat gtcataggaa ggggagaagt aacagggtac acatattgac
     4621 caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt
     4681 ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata
     4741 caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag
     4801 gcaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag
     4861 gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg
     4921 ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct
     4981 cttatcttcc tcccacagct cctgggcaac gtgctggtct gtgtgctggc ccatcacttt
     5041 ggcaaagaat tcgccaccat gggccagact gttaccactc ccttaagttt gaccttaggt
     5101 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     5161 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     5221 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     5281 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     5341 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     5401 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     5461 actccttctc taggcgccaa acctaaacct caagttcttt ctgacagtgg ggggccgctc
     5521 atcgacctac ttacagaaga ccccccgcct tatagggacc caagaccacc cccttccgac
     5581 agggacggaa atggtggaga agcgacccct gcgggagagg caccggaccc ctccccaatg
     5641 gcatctcgcc tacgtgggag acgggagccc cctgtggccg actccactac ctcgcaggca
     5701 ttccccctcc gcgcaggagg aaacggacag cttcaatact ggccgttctc ctcttctgac
     5761 ctttacaact ggaaaaataa taacccttct ttttctgaag atccaggtaa actgacagct
     5821 ctgatcgagt ctgtcctcat cacccatcag cccacctggg acgactgtca gcagctgttg
     5881 gggactctgc tgaccggaga agaaaaacaa cgggtgctct tagaggctag aaaggcggtg
     5941 cggggcgatg atgggcgccc cactcaactg cccaatgaag tcgatgccgc ttttcccctc
     6001 gagcgcccag actgggatta caccacccag gcaggtagga accacctagt ccactatcgc
     6061 cagttgctcc tagcgggtct ccaaaacgcg ggcagaagcc ccaccaattt ggccaaggta
     6121 aaaggaataa cacaagggcc caatgagtct ccctcggcct tcctagagag acttaaggaa
     6181 gcctatcgca ggtacactcc ttatgaccct gaggacccag ggcaagaaac taatgtgtct
     6241 atgtctttca tttggcagtc tgccccagac attgggagaa agttagagag gttagaagat
     6301 ttaaaaaaca agacgcttgg agatttggtt agagaggcag aaaagatctt taataaacga
     6361 gaaaccccgg aagaaagaga ggaacgtatc aggagagaaa cagaggaaaa agaagaacgc
     6421 cgtaggacag aggatgagca gaaagagaaa gaaagagatc gtaggagaca tagagagatg
     6481 agcaagctat tggccactgt cgttagtgga cagaaacagg atagacaggg aggagaacga
     6541 aggaggtccc aactcgatcg cgaccagtgt gcctactgca aagaaaaggg gcactgggct
     6601 aaagattgtc ccaagaaacc acgaggacct cggggaccaa gaccgcaggg atcaggatca
     6661 ggatcaggat caggatctat ggcgactgtc gaaccggaaa ccacccctac tcctaatccc
     6721 ccgactacag aagaggagaa aacggaatct aatcaggagg ttgctaaccc agaacactat
     6781 attaaacatc ccctacagaa cagatgggca ctctggtttt ttaaaaatga taaaagcaaa
     6841 acttggcaag caaacctgcg gctgatctcc aagtttgata ctgttgaaga cttttgggct
     6901 ctgtacaacc atatccagtt gtctagtaat ttaatgcctg gctgtgacta ctcacttttt
     6961 aaggatggta ttgagcctat gtgggaagat gagaaaaaca aacggggagg acgatggcta
     7021 attacattga acaaacagca gagacgaagt gatctcgatc gcttttggct agagacactt
     7081 ctgtgcctta ttggagaatc ttttgatgac tacagtgatg atgtatgtgg cgctgttgtt
     7141 aatgttagag ctaaaggtga taagatagca atatggacta ctgaatgtga aaacagagaa
     7201 gctgttacac atatagggag ggtatacaag gaaaggttag gacttcctcc aaagatagtg
     7261 attggttatc agtcccacgc agacacagct actaagagcg gctccaccac taaaaatagg
     7321 tttgttgttg gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa
     7381 gagaatcctg gaccgatgga gagcgacgag agcggcctgc ccgccatgaa gatcgagtgc
     7441 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     7501 cccgagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     7561 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     7621 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     7681 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     7741 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     7801 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     7861 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     7921 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     7981 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     8041 gtggagtacc agcacgcctt caagaccccg gatgcagatg ccggtgaaga aggatccggc
     8101 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tgggccggcc
     8161 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     8221 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     8281 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     8341 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     8401 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     8461 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     8521 caggactgag aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt
     8581 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8641 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8701 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8761 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8821 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8881 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8941 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9001 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9061 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9121 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     9181 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9241 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9301 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9361 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9421 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     9481 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     9541 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     9601 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     9661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9841 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     9901 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9961 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    10021 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    10081 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    10141 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10561 aaaatttaac gcgaattaat tctgtagctt ggcgtaatca tggtcatagc tgtttcctgt
    10621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11521 acaaaccacc gctggtagcg g
//
