LOCUS       sequence-460291-        8459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NIT-9G domain in N. crassa at the his-3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8459)
  TITLE     Convergent Evolution Links Molybdenum Insertase Domains with 
            Organism-Specific Sequences.
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCCG_nit9G
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5739..5755)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5740..5756)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(join(8458..8459,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(8459,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgaggccta gcggtcccag ctccccgtaa
     2761 ccacgcagcc ccagcgaccg aggtcatcat gacactatga cgacaatctg gcgggagact
     2821 tgggaaaatc gatgacgggc acctaccttg ggaacaatag agcaagtcca gtcaacgccc
     2881 tagggaaggg ggaaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat acgactcact
     4021 atagggcgaa ttggagctcc accgcggtgg cggccgctag aaggagcagt ccatctgcgt
     4081 gaatcacgag agaatcagct actttgaatc gatggatgca gctaccagaa gtcactcagt
     4141 tcgttcaaag ccacatcact gggcacttcc attgggacag gcattgatcg gacgagaccg
     4201 acttctggcc gctttcaaca gccacattat atccatgtca cggctacgcg cggccttcgg
     4261 taacagaaaa gcacacagac agcgattgtg acatggattc gggcaaagca ttggtggtcg
     4321 caccaggtca tcctgagtgt gcagtggctg ctattcagat ttcatctaac tgcgggagag
     4381 gggttcaaag gggcgtgacg tcacagacaa cgggtgaagg acgaagattg cctcacttct
     4441 ttgctagcaa tttgcctgca aagaaggcgc acatgtcaag caaacaactg ggaaaccact
     4501 attgaatacc cacaatgcaa agctcggaag gtacgtcttg attgcagtgt gtcgagtgtc
     4561 aaaaaagaag caagtgttca tgcaagccaa aattggcacc tcctccactt ctccgagtgc
     4621 cccacccgaa cctccaggcg agatggccgg aacataccat ccgcgttggg attatgacgt
     4681 atctccttct tcttcacatg attccatccc gttgttgctt gtttgcgagc tgtgacggga
     4741 gatcgtagat gccacttagg gccaggcagg cagtgcaggc agccaggaac acaagcttcc
     4801 aacttggtca tctcgattgc cgattaaggg aaccaaatgc ctatataaga ctgtcctccc
     4861 acctccccaa taccattctt ttcttcttcc atcatcagcc aacaaagcaa tcacatcttc
     4921 actacttcaa atcaacacaa cactcaaacc actttcacaa cccctcacat caaccaaaat
     4981 gtcctcaagt actactccag cagcgctcaa agcagccatc cttatcgttt caacgacagc
     5041 agctcaagac cccgcaacgg actcggccga cgtcactctc cgccaggtct tcgatgaaca
     5101 acaagcccaa tgggaggtcc atgaatccaa aatcgttccc gacgatgtcg tgcaaatcca
     5161 gaagcaaatc atccactgga caaaccaagc caccgccgca agtggcgaca acaacaacaa
     5221 caacaacacc aacaccatca acctcatcgt aaccacaggc ggcaccggct tcgccgtgtc
     5281 cgaccaaacc accgaagccg tctcggctct cctacacaag aatgctcctg gcctggtcca
     5341 cggcatgctg gcggcttcgc tgcaagtgac cccctttgcc atgatgtcac gacctgtggc
     5401 tggcgtcagg aacggcaccg ttatcgttgc gctgcctggg tcccccaagg gcgcgaagga
     5461 gaatctgcaa gcagtgatca aaatgctgcc ccacgcttgc atgcaggctg ctggtgctaa
     5521 ctctagatga tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     5581 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     5641 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     5701 aagttgggaa gacaacctga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     5761 gggcccggta ccagcttttg ttccctttag tgagggttaa tagggcgtgc acggctatgg
     5821 ggtctgaatg ctaaaagaca ccatttccca cactccctct gatttttttt atggtttttt
     5881 tttatcttca tttttcgccg tcttttattc ggttgtttgg gaggttcaca tgcaacgtgc
     5941 aagactgcaa gtatattgag atgggcgctt catgcaatgc agcatcactg cagcgacatt
     6001 gtgacatcca cgtttcgatc gtctgtgctt ccccgttcat gtacgcatga tctgaaaaaa
     6061 aaaaaaaaaa aaaaaaagtt gatgtaataa tgaaaataaa cacccaggga cgagcacccc
     6121 ttctggaata gggagaaaaa ctaaagggac gtttgggtct ctacacttac caacagattc
     6181 atgtactgat cttcagcctg tacagaacag tacaatgtcc aaaactcgtt atcattactc
     6241 tatgtgccca aaatgaccca gatatacatc aacccactac atccttttgt tcttgttttt
     6301 tttttttttt ttgctacttc gaaaagaatt tccgtttagt tgctcttgct catgtgctca
     6361 agacggatgc tgaccgccct tctgtgagcc tcgagctcct caaccttagc caactgcatg
     6421 acagcctggc cgacgttttt gagaccctcg gcagtcaagt tggagctggt aatgtgcttg
     6481 acgaacgagg cgagattgac gccagagtac tgcttggcaa agccataggt agctatgtgt
     6541 cttgacgtta gcagaagaat ctgtgaagcg gggctattta tgatatgtaa cttacgcagc
     6601 gagtggttaa caccagcaga gtaatcgcca acggactcag gagtccaggc gccaatgaag
     6661 acactaccgg cgttcatgac aagatcgaca gccttctcgg cctccttgat ctggaggatc
     6721 aagtgctcag gagcgtactt gttgctgagc tccatggcct cctcgacggt cttgacctgc
     6781 acggtgatcg agtgggcgat ggagccacgg acaatctgga cgcgaggaag ctccgtagcc
     6841 tgacggtgaa cctcgtcctc aatagcctga agatgctcct cgtcgaggtc aatagcgatc
     6901 aggatgacct gactgtcaac gccgtgctca gcctgggaca ggagatccga ggcaacgaac
     6961 gcggggttgg cgtccttgtc agcgatgacc agcacctcgg acgggccagc gggcatgtca
     7021 ataccaacgg cagcgttggt gtcgttgctg acgaacatct tggcagcagt gacgaactgg
     7081 ttaccggggc cgagaatctt gtcgaccttg gtgatgctct cggtgccgta ggccatggca
     7141 gctacggcct gggcaccgcc ggcaagcacg atggactcgg cgccaacctt gtgagcgacg
     7201 tagacaatct cgggagtgat ggttccgtcg gcgcggggag gagaggcgaa cacaatcttg
     7261 ttgcagccgg cgaccatggc gggaacaccc agcataaggg cagtgctggg gagaacggcg
     7321 gtaccgccgg ggatgtagca gccgacggcc tcgatgggac gagagaaacg gctgcagaca
     7381 acaccgggca tggtctcgac ctggaggggc ttctcctcct tctgggcggc gtggaacttg
     7441 cggatgttct cgaaggacac gtcgatggca gcaatggtct cctcagggag ctgcataagc
     7501 tccttgggga agggcgcctt caggacgggg ctagtaagag aggtagcctt ctcgaacttg
     7561 tgagtgtacg acagaacagc cttgtcgccg ttcttgcgga cgtcctcgat gatggggaca
     7621 atgatcttgt agatggcatc ggacgacttt tgcgcaggac gcttgagagc agcatcgagc
     7681 tcctcggtag agaccttgga ggcgtcgaaa cgtctcatgg tgatcttctc aggggtggtc
     7741 tcctgggcgg cctccttggt gacaggggcc gaagtggcag cgagagcgga cgccgcaggc
     7801 ttgatgccct ccttctcagc ccacttaccc ttagcatctc cagtcctgcg cttgaccttc
     7861 cagctcttgg cgtcaaggct cctttcgata tcggcaagag taacgccggc agcaacggcc
     7921 ctggtaagag caaagtagaa gagatcggca gcctcaaagg cgatttcctg gggggtctga
     7981 gcggtgcaga gctcctcagc ctcctccatg atcttggccc ggactagctt ctcatcggag
     8041 aagagacggg cagtgtagga gccctcgggg gcagactgtt tcctcgaaat caaagtctgc
     8101 tcgagcttgg gaaggccttt gagctgacca aagcagccgg actgatcgag gtggcagaaa
     8161 cgacccttct gcttcacgac aaacttgaga gcatcgttat cgcagtcaag cgagatgcgg
     8221 acgagctcct gagtgtctcc ggaagtagca cccttgtacc agagaccgcg cttccggctc
     8281 tgatagacac cagtctgtgt cctgagggcc tcgttcacac tctcggcact gctgtagacc
     8341 agacccagag cagtgtcgtg ctcatcaacg acaacggtgg ggagaagacc atcgggacgg
     8401 tcagacttcc agacgctcga gaggatggtg gagatggcaa gcttcagctg ctcgagttc
//
