LOCUS       sequence-460294-       10136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NIT-9 domains in reverse orientation with Gephyrin 
            linkage region in N. crassa at the his-3 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10136)
  TITLE     Convergent Evolution Links Molybdenum Insertase Domains with 
            Organism-Specific Sequences.
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCCG_nit9fl_Gephyrin_linker_reverse
FEATURES             Location/Qualifiers
     source          1..10136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(800..816)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(801..817)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(3518..3536)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3519..3536)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3617..3636)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3736..3758
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3796..3814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3882..3986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3987..4055,4056..4847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4205..4224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5018..5606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5507..5526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5760..5777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5864..5882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6157..6168
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7527..7545
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     8499..8515
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8499..8515
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cagatgaacg gattccagac tccatcattt ctcgtggtgt tcaggtgctc ccacgagaca
       61 cagcctccct cagcactact ccttcagaat cgcctcgtgc tcaggctaca tctcgcctct
      121 ctacagcttc ctgcccaaca ccaaaagtcc agtccaggtg cagcagcaag gagaacattc
      181 tcagagccag tcacagtgct gtcgatatca ccaaggtggc tagaagaatg tcctcaagta
      241 ctactccagc agcgctcaaa gcagccatcc ttatcgtttc aacgacagca gctcaagacc
      301 ccgcaacgga ctcggccgac gtcactctcc gccaggtctt cgatgaacaa caagcccaat
      361 gggaggtcca tgaatccaaa atcgttcccg acgatgtcgt gcaaatccag aagcaaatca
      421 tccactggac aaaccaagcc accgccgcaa gtggcgacaa caacaacaac aacaacacca
      481 acaccatcaa cctcatcgta accacaggcg gcaccggctt cgccgtgtcc gaccaaacca
      541 ccgaagccgt ctcggctctc ctacacaaga atgctcctgg cctggtccac ggcatgctgg
      601 cggcttcgct gcaagtgacc ccctttgcca tgatgtcacg acctgtggct ggcgtcagga
      661 acggcaccgt tatcgttgcg ctgcctgggt cccccaaggg cgcgaaggag aatctgcaag
      721 cagtgatcaa aatgctgccc cacgcttgca tgcaggctgc tggtgctaac tctagataag
      781 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt accagctttt
      841 gttcccttta gtgagggtta atagggcgtg cacggctatg gggtctgaat gctaaaagac
      901 accatttccc acactccctc tgattttttt tatggttttt ttttatcttc atttttcgcc
      961 gtcttttatt cggttgtttg ggaggttcac atgcaacgtg caagactgca agtatattga
     1021 gatgggcgct tcatgcaatg cagcatcact gcagcgacat tgtgacatcc acgtttcgat
     1081 cgtctgtgct tccccgttca tgtacgcatg atctgaaaaa aaaaaaaaaa aaaaaaagtt
     1141 gatgtaataa tgaaaataaa cacccaggga cgagcacccc ttctggaata gggagaaaaa
     1201 ctaaagggac gtttgggtct ctacacttac caacagattc atgtactgat cttcagcctg
     1261 tacagaacag tacaatgtcc aaaactcgtt atcattactc tatgtgccca aaatgaccca
     1321 gatatacatc aacccactac atccttttgt tcttgttttt tttttttttt ttgctacttc
     1381 gaaaagaatt tccgtttagt tgctcttgct catgtgctca agacggatgc tgaccgccct
     1441 tctgtgagcc tcgagctcct caaccttagc caactgcatg acagcctggc cgacgttttt
     1501 gagaccctcg gcagtcaagt tggagctggt aatgtgcttg acgaacgagg cgagattgac
     1561 gccagagtac tgcttggcaa agccataggt agctatgtgt cttgacgtta gcagaagaat
     1621 ctgtgaagcg gggctattta tgatatgtaa cttacgcagc gagtggttaa caccagcaga
     1681 gtaatcgcca acggactcag gagtccaggc gccaatgaag acactaccgg cgttcatgac
     1741 aagatcgaca gccttctcgg cctccttgat ctggaggatc aagtgctcag gagcgtactt
     1801 gttgctgagc tccatggcct cctcgacggt cttgacctgc acggtgatcg agtgggcgat
     1861 ggagccacgg acaatctgga cgcgaggaag ctccgtagcc tgacggtgaa cctcgtcctc
     1921 aatagcctga agatgctcct cgtcgaggtc aatagcgatc aggatgacct gactgtcaac
     1981 gccgtgctca gcctgggaca ggagatccga ggcaacgaac gcggggttgg cgtccttgtc
     2041 agcgatgacc agcacctcgg acgggccagc gggcatgtca ataccaacgg cagcgttggt
     2101 gtcgttgctg acgaacatct tggcagcagt gacgaactgg ttaccggggc cgagaatctt
     2161 gtcgaccttg gtgatgctct cggtgccgta ggccatggca gctacggcct gggcaccgcc
     2221 ggcaagcacg atggactcgg cgccaacctt gtgagcgacg tagacaatct cgggagtgat
     2281 ggttccgtcg gcgcggggag gagaggcgaa cacaatcttg ttgcagccgg cgaccatggc
     2341 gggaacaccc agcataaggg cagtgctggg gagaacggcg gtaccgccgg ggatgtagca
     2401 gccgacggcc tcgatgggac gagagaaacg gctgcagaca acaccgggca tggtctcgac
     2461 ctggaggggc ttctcctcct tctgggcggc gtggaacttg cggatgttct cgaaggacac
     2521 gtcgatggca gcaatggtct cctcagggag ctgcataagc tccttgggga agggcgcctt
     2581 caggacgggg ctagtaagag aggtagcctt ctcgaacttg tgagtgtacg acagaacagc
     2641 cttgtcgccg ttcttgcgga cgtcctcgat gatggggaca atgatcttgt agatggcatc
     2701 ggacgacttt tgcgcaggac gcttgagagc agcatcgagc tcctcggtag agaccttgga
     2761 ggcgtcgaaa cgtctcatgg tgatcttctc aggggtggtc tcctgggcgg cctccttggt
     2821 gacaggggcc gaagtggcag cgagagcgga cgccgcaggc ttgatgccct ccttctcagc
     2881 ccacttaccc ttagcatctc cagtcctgcg cttgaccttc cagctcttgg cgtcaaggct
     2941 cctttcgata tcggcaagag taacgccggc agcaacggcc ctggtaagag caaagtagaa
     3001 gagatcggca gcctcaaagg cgatttcctg gggggtctga gcggtgcaga gctcctcagc
     3061 ctcctccatg atcttggccc ggactagctt ctcatcggag aagagacggg cagtgtagga
     3121 gccctcgggg gcagactgtt tcctcgaaat caaagtctgc tcgagcttgg gaaggccttt
     3181 gagctgacca aagcagccgg actgatcgag gtggcagaaa cgacccttct gcttcacgac
     3241 aaacttgaga gcatcgttat cgcagtcaag cgagatgcgg acgagctcct gagtgtctcc
     3301 ggaagtagca cccttgtacc agagaccgcg cttccggctc tgatagacac cagtctgtgt
     3361 cctgagggcc tcgttcacac tctcggcact gctgtagacc agacccagag cagtgtcgtg
     3421 ctcatcaacg acaacggtgg ggagaagacc atcgggacgg tcagacttcc agacgctcga
     3481 gaggatggtg gagatggcaa gcttcagctg ctcgagttct atagtgtcac ctaaatcgta
     3541 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtcca
     3601 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3661 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3721 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3781 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     3841 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4201 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5821 gttggccgat tcattaatgc aggttaacct ggcttatcga aattaatacg actcactata
     5881 gggagaccgg cagatccaat gcggatggat tcgctcactc ggtgccgcgg cggtgcgaag
     5941 ttcctgccgt ggattggggg attgcacaga cggtggagtg cttgctacag ctaggtaccc
     6001 agttgcacac caaacggtgc ccctgtgccc ctgcgtctag gtgtgtcctg tgagcaactg
     6061 cgcccgcgcg cggcaccggg aagctgcggg aacctcaatg gatgattggc gccagatccc
     6121 cggcatcacc tcagcacatg ggcagcccga gaaggaatcg agggtagaag atattccagg
     6181 gtttccgcta gcacaacttc caagcgcctc taccgctact ccacacaccc acgctgaatg
     6241 gcacaaccac tgaggcctag cggtcccagc tccccgtaac cacgcagccc cagcgaccga
     6301 ggtcatcatg acactatgac gacaatctgg cgggagactt gggaaaatcg atgacgggca
     6361 cctaccttgg gaacaataga gcaagtccag tcaacgccct agggaagggg gaaaaaaaaa
     6421 aaaagagccc attctcacaa ggagcaacac tgtgctgcta tcatagtaca ctacaagcaa
     6481 agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga tacctcccat aggcagtaag
     6541 tctaggtatc caatttatag ttccgcgtat ttcttcgtcg aacggggcac agcaaagcct
     6601 aacagccgca gtgcctcgca accaacacca agcagtaatc tcatccatcc atacagatcc
     6661 tcccacaata tctttacatg cgcacttgcg ctgtgttagc agtttaggtt ccactaccaa
     6721 ggctgacacg agcagcactg acagcaaact gctgcttggg gccaagaaat tggcagtttg
     6781 ctgagcacct acatatcagt acacatcaac cctggtttaa ttgttagcct ggtactgtta
     6841 accgtcatga acgaggacgt ggaggaagaa ccacgctaac aattgaacca gggttgatgt
     6901 atacctctct taccatattt gcttgcttcc acgtccctat cctcaagcga ttttgttctt
     6961 gactttttgt atttcaacgc aaaggccatt caggtttctc atgttcaacc ctttggatgg
     7021 cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac gagaaatata tcccaaagca
     7081 acttctgctt atggacaagc aactgcatgg cggcccacaa ctcatatctc gcgtaatcat
     7141 ccaaacgcta ggcccgagta ctctactgtt ctcgagacac ctaccataat atgtacccaa
     7201 tcagacatga caacagtcaa ccaaacatac cctgatccgg ccaaaccctg acatccctag
     7261 atgatggtta ggcacacaaa tggaaaacgg gactcgttgg ttaggtggga acgcttgtat
     7321 tgcagccagc caaccgactc tcctcgtccc tctcgagtcc cgttattgcc gtttgaccca
     7381 gccgcccagc cgccaaacag ctgagggagc caatggtcta atacaccgtc caacggcccc
     7441 agtgatgttt caaacttgat tgacagcgaa cgaaacccct gaaacccgtt atagatatgt
     7501 atagatttga cggggtagct tggccctaat acgactcact atagggcgaa ttggagctcc
     7561 accgcggtgg cggccgctag aaggagcagt ccatctgcgt gaatcacgag agaatcagct
     7621 actttgaatc gatggatgca gctaccagaa gtcactcagt tcgttcaaag ccacatcact
     7681 gggcacttcc attgggacag gcattgatcg gacgagaccg acttctggcc gctttcaaca
     7741 gccacattat atccatgtca cggctacgcg cggccttcgg taacagaaaa gcacacagac
     7801 agcgattgtg acatggattc gggcaaagca ttggtggtcg caccaggtca tcctgagtgt
     7861 gcagtggctg ctattcagat ttcatctaac tgcgggagag gggttcaaag gggcgtgacg
     7921 tcacagacaa cgggtgaagg acgaagattg cctcacttct ttgctagcaa tttgcctgca
     7981 aagaaggcgc acatgtcaag caaacaactg ggaaaccact attgaatacc cacaatgcaa
     8041 agctcggaag gtacgtcttg attgcagtgt gtcgagtgtc aaaaaagaag caagtgttca
     8101 tgcaagccaa aattggcacc tcctccactt ctccgagtgc cccacccgaa cctccaggcg
     8161 agatggccgg aacataccat ccgcgttggg attatgacgt atctccttct tcttcacatg
     8221 attccatccc gttgttgctt gtttgcgagc tgtgacggga gatcgtagat gccacttagg
     8281 gccaggcagg cagtgcaggc agccaggaac acaagcttcc aacttggtca tctcgattgc
     8341 cgattaaggg aaccaaatgc ctatataaga ctgtcctccc acctccccaa taccattctt
     8401 ttcttcttcc atcatcagcc aacaaagcaa tcacatcttc actacttcaa atcaacacaa
     8461 cactcaaacc actttcacaa cccctcacat caaccaaatc tagaactagt ggatccccgg
     8521 gttaattaac atgctctctg tctccgaagc gctctcccaa atagcgcaac acacgccgat
     8581 gccgcaaatc gtcacctcca aagtcgatag ctcgctcatc ggcgccgttc tggcgcagga
     8641 cgtttcggcg cgcgaaaatg tgcccgcttt tcgcgctagc atcgtcgacg gctacgccgt
     8701 agtcgtgccc caagacggga acatgcgcgg cgtgttcccc gtcgtgtcgg tttcgcatgc
     8761 cgctcctgga gatggggaaa aaatgccgac gctcaaagag ggcgagattg cccggatcac
     8821 cacgggtgca cccctgcctc cgggtgcgac atcggttatt atggtggagg atacggtgtt
     8881 ggtgtcgacg acgtcatcca agacggagga tgcggaggat ggaaagggag tggaggagga
     8941 gaaggaggtg gagattttag cagctgataa agttcgcgaa ggcgaaaatg ttagggaggt
     9001 gggaagtgat atcaagcagg ggcaactgat tctaggcaag ggagatcagg tgtctgctgt
     9061 ggggggtgag attggtctgt tggccgccgt gggtgtgggt gaggtgcagg tgtaccgacg
     9121 tcctgttgtt ggcgttctat ctacgggtga tgagattgtg gaccatgatc gcccgggaga
     9181 cttgcggctg ggcgaggtgc gcgatacgaa ccggccaact ctcatttcgg cggcgagaga
     9241 ttgggggtat gaggtgattg atcttggagt ggcgcgggac aaggccggga ctttggagga
     9301 gacgctgcgg caggcattga ggcaggttga cgtggtgatc acgaccggcg gagtgtccat
     9361 gggtgagctg gatctcctca agccgacgat agagcgttcg ctgggcggaa caattcgctt
     9421 tggtcgggtg gcaatgaagc ccggcaagcc taccacgttt gctaccgttc cggtaaagga
     9481 taacgacgga gagagagttt ccaaggtaat cttctcgcta ccaggtaacc cggcatcggc
     9541 tttggttacg tttcacttgt ttgtactgcc gtctctgcac caaatgtcgg gcgtctcacc
     9601 acctggcttg ccaaaggtgc ctgtgacact gggccatgat ttctccctcg atagatctag
     9661 acccgagtac caccgggcca ttgtctccgt tagcagggac ggagtgctca ccgcggtcag
     9721 cacaggagga cagagaagct cgcgggtagg aagtatgaag ggggccaacg cgcttctctg
     9781 catgccttgc ggaccagagc cgttgcgcaa gggtgccaag gtggaagcgt tgcttatggg
     9841 cggtttacgc agcgatgtga ttgaacttga agatttgcct tccccacctc cccctctttc
     9901 ccctcctcct actaccagcc cccataaaca gacagaagac aaaggagttc aatgtgagga
     9961 agaggaagaa gagaagaaag acagtggtgt tgcttcaaca gaagatagtt cctcatcaca
    10021 tataactgca gcagccattg ctgccaagaa gcatccattc tacaccagtc ctgctgttgt
    10081 catggcacac ggtgaacagc ccatccctgg tctcatcaat tattcccatc attcaa
//
