LOCUS       sequence-460296-       13199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CTDSP2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:CTDSP2_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    7372..7506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7527..7661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7963..8676,8677..8679,8680..8682))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7966..8676,8677..8679,8680..8682))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8008..8029)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8357..8376
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8616..8637
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9863..9879)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9863..9879)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9934..9952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9962..9979)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9962..9978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9971..9993)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10119..10574)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10256..10277)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10468..10487
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10601..10705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10706..10774,10775..11566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10924..10943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11737..12325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12226..12245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12479..12496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12613..12634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12649..12654,12655..12672,12673..12679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12687..12703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12692..12714
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12711..12727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12711..12727
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12746..12766
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12748..12766
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatggg
      721 aaagctaggt tgctcccgga agaggtgtta gtaaaccagc aggtctacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat aaaccagcag gtctacctgt ggtctgtgtg ggtcctaatg ccccagtata
      901 aaccagcagg tcaacctgta gtctgtgtgg gtcctaatgc cccagtataa accagcaggt
      961 ctacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccaacaggtc aacctgtgat
     1021 ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
     1081 ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca
     1141 gtataaacca gcaggtcaac gtgtggtctg tgtgggtcct aatgccctgg tataaaccag
     1201 caggtcaact tgtggtctgt gtgggtccta atgccccggt ataaaccagc atgtcaacct
     1261 gtggtctgtg tgggtcctaa tgccccggta taaaccagca gatcaacctg tggtctgtgt
     1321 gggtcctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg
     1381 ccccggtaca aaccagcatg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa
     1441 accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc
     1501 tacctgtggt ctgtgtgggt cctaatgccc cggtacaaac cagcatgtca acctgtggtc
     1561 tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc
     1621 taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct aatgccccag
     1681 tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc
     1741 aggtctacct gtggtctgtg tgggtcctaa tgcaccagta taaaccagca ggtcaacctg
     1801 tggtctgtgt gggttctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg
     1861 ggtccaaatg ccccagtaca aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc
     1921 cccagtataa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccggtacaaa
     1981 ccagcatgtc aacctgtggt ctgtgtgggt cctaatgccc cggtataaac cagcagatca
     2041 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcagatcaa cctgtggtct
     2101 gtgtgggtcc taaagtccca ggataaacca gcatgtcaac ctgtggtctg tgtgggtcct
     2161 aatgccccgg tataaaccag cagatcaacc tgtggtctgt gtgggtccta atgccccagg
     2221 ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca
     2281 ggtcaacctg tggtctgtgt gggtcctaat gccccagtat attgatggag actctatact
     2341 gctcggtgag ctccatcttt cggataagat gttaaaacaa ggtcccgact ctctgtggtg
     2401 gtaaaaaatt cccaggatgt ccttcctacc ctggcatcct ggacaaattt gctcactgtc
     2461 ctctgtccat catggcctcc taaccatccc catatcataa ttggctttat cactctagct
     2521 ggggccgaaa tcgcatactt ccattttatg aggtacgcta aaatcagtat gcgagccaag
     2581 taatatgtcc gaatacttcc ggcgagattc tagagtgtgc attcgatgca cgctacgcta
     2641 ttccatgatg ccccgtgaga gaattcttga atcggagaga agcaacgcaa ctcatgcggg
     2701 taggttacgt gacgatgaca tattggcgga tgtagtacgt ccaagttcca ttcatactgc
     2761 tcacattcat attttataga atgttctttt cccacagcca agtagtaagt ttaaattcaa
     2821 atacagtacc tactaagtag tagtagacgg tttcggacgc agcccctgtc tcctctccac
     2881 caatatactg gtgtgtagtg tgcggtctgg tgcaatatgg ctgccatcgc gtcatccagg
     2941 tggatgctgc acactggtgg ttgatgagga gattttgccc aaaaaagtgt tttgagtttc
     3001 cagaaaagcg cgatataaat gtaaggaatt atcattagct tattataatg gaactttctg
     3061 gataacttgt gccagagcta ggggccatta aaactttgcc ctggggtacc tcagggagct
     3121 gcaaggaagt gctatagggc taatgaaatg tttgggggaa aaaaaaaaat tttataagta
     3181 aaaataaaac taaaataatt aataatttca ttgttgaaaa ttatagtaaa attgataaac
     3241 aagataagca gcattagtat aaaaaactaa aaacaaacgt aatttaaaat aattaaattg
     3301 ttttaaataa attatacaaa ataatgttta caacgacacg gtggtgcagt aggtagtgct
     3361 gtcgccagca aagcaagaag gtcgctggtt cgagcctcag ctggatcact tgatgtttct
     3421 gtgtggagca ttcgcgtgcg tttaacccca gattaataaa gagactaagc cgaaaagaaa
     3481 ataaatgtat gaaaatgttt acgtaaaatg taaataaatg catgattaat aaaatatatg
     3541 aattttaaag attgaggtag aattgtttta ttattcacac atttggttat ttttaatcag
     3601 tgacaacttg tgatgctctt tatattgttt attacgctac tttgaatatt cagtctcaaa
     3661 ttgcatgtaa atacgacttc aaagcagaat tagtttaatt taattgcctg taaacagtgt
     3721 tgtactttga tggtgattgt ctgataataa tatgatttca ctatttaaaa tttgaaaaaa
     3781 aaaaatctga aaaatactca ccggccactt tattaggtac acctgtctaa ctgcttgtta
     3841 acgtaaattt ctaatcagcc aatcaccagg cagcaactca gtgcatttag gcatgtagac
     3901 atggtcaaga cgatctgctg cagttcaaag cgagcatcag aatggagaag caaggtgatt
     3961 taagtgactt taaacgtgac gtgtttgttg gtgccagacg ggctggtctg agtatttcag
     4021 aaactgctga tttactgggg ttttcacaca caaacatctc tggggtttac agagaatggt
     4081 ccaaaaaaag ataatatcca gtgagcggca gttctgtggg ggcaaatgtc ttgttgatgc
     4141 cagaggtcag aggagaatgg cctgactggt tccagctgat agaaaggcaa cagtaattca
     4201 aataaccact cgttagaact gaggcatgca gaagagcacc tttgaacaca acatgtcgaa
     4261 ccttgaggcg ggtgggctac agcagctcaa gaccacatcg ggtgccactc ctgtcagcta
     4321 agaacaggaa actgaggcta caattcgcac aggctcaaca taagtagaca atagaagatt
     4381 gaacattatg ttgcctggtc agatggatac attctacctt gtatcaacag ttcaggctgg
     4441 tgatggtggt gtaatggtgt gggggatata ttcttggcac actttgggcc cattagtacc
     4501 aattaaccat cgtgtcaacg ccacagccta actgagtatt gttgctgacc atgttcattt
     4561 ctttttgacc acagtgtacc catcttctga tggctacttc ctgcaggata atgcaccatg
     4621 tcataaagcg tgaatgatct cagactggtt tcttgaacat gacaatgagt tcactgtgct
     4681 cgaatgacct ccacagtcac cagatctcaa tccaatagag cgcctttggg gtgtggtaga
     4741 atggaagatt cgcatcctgg atgtgcagcc gacaaatctg catgatgcta gcatgtcaat
     4801 atggaccaaa atctctgagg aattttccag tacctggttg aaatgcaaaa ggaagggaat
     4861 ctatgctaag gcaaaaagag gtccaaccca gtactagtgg ggtgtacata ataaagtggc
     4921 cggtgagtgt atattgttaa ggatatctag ataatccaaa taatttacag cactatttaa
     4981 aaaaaaaact ttatgtcacc aagtctgcat ttatttgatc acaattacag tcgaaaaagc
     5041 aatatcggga tacattgttt ccatttaaaa taactatatt atattttaaa tatttttgac
     5101 aatgttattt ctttaatgcc aaagctaaat tgtattcaat ctgaatttta gtgtcacatg
     5161 atccttcaga aaatattaac acattgctaa aaatgtatat tattatcaat ttggattttt
     5221 tttttttttt tgtatattaa cctatgctat tggttactat acaaaagtat tactgtgaat
     5281 atatgcatat acagaaccgt atcaagcgcg gggggtggtt gggttatgtc cccatttgga
     5341 aagacctgga aacattcata atgactaaaa ttgcacggaa aaatataata tctatgagta
     5401 ttgctgatca gcagcacaaa accagaactc catattaata aacagccaat gatgcagtga
     5461 gtttgtgcat gttttcattc aaactcgtgt gattgtgctc aaacacctgt tgtgagcagc
     5521 agttgcaaac agtaaacctc aagaggagcc agtcaacctt cacaaacact caacctctgg
     5581 ctcactcact aaacaattcg cgttaatatc taaaataaaa atttccagca ccaaacagct
     5641 ctcagatttc ggagtacact gaccaagagg taagaagtaa cgttacatta ctaggacgac
     5701 tattgtaaga ctagatatgt aatatttgga agcgtacgtg ttttaagtga catatagagt
     5761 agaggaactg atggaaatac gatttgcata atgtaaaatg atatatatta aaatataggc
     5821 tacagtaggt aacgtggttt acatataacg ttactgtgtt aatgtagtgc tcagttactc
     5881 gtgttgacag ttattattag tgcttgctaa tttgtttgtt aatgcttctc ttgcgtattt
     5941 gattaccttc aggcgaatct aaaacatatc agatgtagaa acggcagaaa aatgttgaag
     6001 aaaatacgag aatagtagct tttggagcac atagaaaagt acatcagata aagcctaccg
     6061 tttaaacgca gacgccgctc tcgcactgaa tgctgggtac tgtagtcctt atattgtatg
     6121 cttaatgtgc gtctcaacgg gaaactagag ttaggaaaac tacaatttcc tgtggaaaat
     6181 gtttctgttt tcggggaaac agcgaaagaa agatatggtt ccgcgttttt ttcttacttt
     6241 gaataataaa ataagaccta actacacact tcgcttactc ttatgctaat ttgaagcact
     6301 agcattcttt gatacaatga taatcattta aacatcacag ttatactaca aatactatgg
     6361 ttaaactatg tctagtgtag tgtaacacta tggttgccat tgttatcttt aagtttaagt
     6421 attatctgtg gtaaagccat ggttggtttt tataagaggt aataaaatta acattgtaca
     6481 attaaatgac tataaattaa tgtttttttt tatttttttt acacagctac cttcaaaaag
     6541 ggatccatgg aacacggctc catcatcacc caggcgcgga gggaagacgc cctggtgctc
     6601 accaagcaag gcctggtctc caagtcctct cctaagaagc ctcgtggacg taacatcttc
     6661 aaggcccttt tctgctgttt tcgcgcccag catgttggcc agtcaagttc ctccactgag
     6721 ctcgctgcgt ataaggagga agcaaacacc attgctaagt cggatctgct ccagtgtctc
     6781 cagtaccagt tctaccagat cccagggacc tgcctgctcc cagaggtgac agaggaagat
     6841 caaggaagga tctgtgtggt cattgacctc gatgaaaccc ttgtgcatag ctcctttaag
     6901 ccaatcaaca atgctgactt catagtgcct atagagattg aggggaccac tcaccaggtg
     6961 tatgtgctca agaggcctta tgtggatgag ttcctgagac gcatggggga actctttgaa
     7021 tgtgttctct tcactgccag cctggccaag tatgccgacc ctgtgacaga cctgctggac
     7081 cggtgtgggg tgttccgggc ccgcctattc cgtgagtctt gcgtgttcca ccagggctgc
     7141 tacgtcaagg acctcagccg cctggggagg gacctgagaa agaccctcat cctggacaac
     7201 tcgcctgctt cttacatatt ccaccccgag aatgcagtgc ctgtgcagtc ctggtttgat
     7261 gacatggcag acactgagtt gctgaacctg atcccaatct ttgaggagct gagcggagca
     7321 gaggacgtct acaccagcct tgggcagctg cgggcccctt agatcgatga tgatccagac
     7381 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     7441 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     7501 caagttaaca acaacaattc gatgatgatc cagacatgat aagatacatt gatgagtttg
     7561 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     7621 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     7681 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     7741 acaaatgtgg tatggctgat tatgatcctc tagatcagat ctcttgttta ttgcagctta
     7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctacgt
     7921 aatacgactc actatagttc tagaggctcg agaggggccg ctttacttgt acagctcgtc
     7981 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     8041 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
     8101 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     8161 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     8221 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     8281 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     8341 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     8401 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     8461 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     8521 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     8581 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     8641 gggcaccacc ccggtgaaca gctcttcgcc cttgctcacc atggtcactg tctgctttgc
     8701 tgttggtctg ggctcctggg tcactggctt actaatggag tctttatgta tgaggactct
     8761 tatcaattgt tcttctataa aggtctgcag tgtttctgtt cgtcccctac atggacaccc
     8821 agagcctcct aaatacagga gccctgataa ctgcacaagt gctcagattc cagcagggtg
     8881 gaaatgagat aaagtgtgca gatggggagg gggacgtgaa tgagagattt gagggatgaa
     8941 aaggatggat gaacgcattg aaaatagccc ctttcacaaa ataataccag taaattgtca
     9001 tataattaat gaccagacct ttacctgtaa atgatgtgat gttcatatac agaagaatgc
     9061 caacaattta cagatgattt tacaaattct taattttcct tttctaaatg gatttgttca
     9121 cacattatct cttcaaagca attgaatttt ctggaaaaga ttggctgtgt gtgtaaaagg
     9181 tgctactgag gaatttaatg tgacatggaa tgaagcaaac agcagcctaa gacaggatgg
     9241 caggaaaaat gtcacatgtt taaaataaga gtgaaaccaa cgacctggac ttatagagac
     9301 tgtgtgtcct taggcaaagc tcttgtacaa acagtgcttg tgactgtgaa attcggggtt
     9361 tgcctggatt gtgttaaatg tgtgcttgga tgtatcataa aaaagaatcg gttctctgta
     9421 tttgacattt tctgtaattt atctaaagtt acaactggct atgcctgatt tttatcacta
     9481 ttgaatcaat cattttaaag aatgttaagt ctgctgattt ggagagctca tttacattta
     9541 ttttgaatgt ctcttattta acacaactga tttaagcttt agatctgcga agatacggcc
     9601 acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat
     9661 cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt
     9721 caaggtgctg tgcattgtgg taatagatgt gacatgattg cattcatttt atgtttcagg
     9781 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     9841 ctgattatga tcctctagat cagatccact agttctagag cggccattac cctgttatcc
     9901 ctaggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat tacgcgcgct
     9961 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    10021 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    10081 gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt
    10141 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
    10201 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
    10261 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
    10321 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
    10381 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
    10441 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    10501 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    10561 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    10681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    10741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    10801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    10861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    11041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    11101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    11161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    11221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    11281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    11341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    11401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    11461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    11521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    11581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    11701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    11941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    12001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    12061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    12121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    12181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    12241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    12301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    12361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    12421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    12481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    12541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    12601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    12661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    12721 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctggg
    12781 taccgggccc cccctcgaat taccctgtta tccctatcga gcaaaatcaa tcctaatgta
    12841 aaatagtata cgttcatcat tataatgcaa acactgtaga actgttgtgt tgtgccttgc
    12901 atttcattct cagtttctta tcgtctgtct ctttttacga cagagctaga aaaaactagc
    12961 cttcatttgc catcgccgta tgcaaatcca ctccagaact caacaggttt gttctgcggc
    13021 gggtgtaagt gtgaacaccg gcatgtaagt gcgcttgagc aggagaataa agcgctcaga
    13081 gacaccggag gaatccttta ttttgcgttg cgcctcaggg gattcgctga gccttctctt
    13141 catggccctg ctcttcagac acaggtgctc cgctgtttgt gtttttcact gttcttcag
//
