LOCUS       sequence-460299-       12271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MARCH--9 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12271)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:MARCH9_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    33..114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(416..1129,1130..1132,1133..1135))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(419..1129,1130..1132,1133..1135))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(461..482)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     810..829
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1069..1090
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2316..2332)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2316..2332)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2387..2405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2415..2432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2415..2431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2424..2446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2572..3027)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2709..2730)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2921..2940
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3054..3158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3159..3227,3228..4019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3377..3396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4190..4778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4679..4698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4932..4949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5066..5087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5102..5107,5108..5125,5126..5132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5140..5156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5145..5167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5164..5180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5164..5180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5199..5219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5201..5219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gap             6547..6646
                     /estimated_length=100
ORIGIN
        1 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
       61 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      121 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
      181 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatctcttgt
      241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      301 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      361 tctggatcta cgtaatacga ctcactatag ttctagaggc tcgagagggg ccgctttact
      421 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      481 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
      541 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      601 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
      661 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
      721 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      781 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      841 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      901 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
      961 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     1021 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     1081 gctcgaccag gatgggcacc accccggtga acagctcttc gcccttgctc accatggtca
     1141 ctgtctgctt tgctgttggt ctgggctcct gggtcactgg cttactaatg gagtctttat
     1201 gtatgaggac tcttatcaat tgttcttcta taaaggtctg cagtgtttct gttcgtcccc
     1261 tacatggaca cccagagcct cctaaataca ggagccctga taactgcaca agtgctcaga
     1321 ttccagcagg gtggaaatga gataaagtgt gcagatgggg agggggacgt gaatgagaga
     1381 tttgagggat gaaaaggatg gatgaacgca ttgaaaatag cccctttcac aaaataatac
     1441 cagtaaattg tcatataatt aatgaccaga cctttacctg taaatgatgt gatgttcata
     1501 tacagaagaa tgccaacaat ttacagatga ttttacaaat tcttaatttt ccttttctaa
     1561 atggatttgt tcacacatta tctcttcaaa gcaattgaat tttctggaaa agattggctg
     1621 tgtgtgtaaa aggtgctact gaggaattta atgtgacatg gaatgaagca aacagcagcc
     1681 taagacagga tggcaggaaa aatgtcacat gtttaaaata agagtgaaac caacgacctg
     1741 gacttataga gactgtgtgt ccttaggcaa agctcttgta caaacagtgc ttgtgactgt
     1801 gaaattcggg gtttgcctgg attgtgttaa atgtgtgctt ggatgtatca taaaaaagaa
     1861 tcggttctct gtatttgaca ttttctgtaa tttatctaaa gttacaactg gctatgcctg
     1921 atttttatca ctattgaatc aatcatttta aagaatgtta agtctgctga tttggagagc
     1981 tcatttacat ttattttgaa tgtctcttat ttaacacaac tgatttaagc tttagatctg
     2041 cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca
     2101 gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc
     2161 ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga ttgcattcat
     2221 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     2281 aaatgtggta tggctgatta tgatcctcta gatcagatcc actagttcta gagcggccat
     2341 taccctgtta tccctaggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg
     2401 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     2461 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     2521 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa
     2581 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2641 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2701 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2761 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2821 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2881 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2941 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3001 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3061 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3841 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4201 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4261 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4321 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4381 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4441 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4501 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4561 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4621 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4681 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4741 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4801 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4861 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4921 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4981 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5041 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5101 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5161 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     5221 aacaaaagct gggtaccggg ccccccctcg aattaccctg ttatccctat cgagcaaaat
     5281 caatcctaat gtaaaatagt atacgttcat cattataatg caaacactgt agaactgttg
     5341 tgttgtgcct tgcatttcat tctcagtttc ttatcgtctg tctcttttta cgacagagct
     5401 agaaaaaact agccttcatt tgccatcgcc gtatgcaaat ccactccaga actcaacagg
     5461 tttgttctgc ggcgggtgta agtgtgaaca ccggcatgta agtgcgcttg agcaggagaa
     5521 taaagcgctc agagacaccg gaggaatcct ttattttgcg ttgcgcctca ggggattcgc
     5581 tgagccttct cttcatggcc ctgctcttca gacacaggtg ctccgctgtt tgtgtttttc
     5641 actgttcttc aggcactcgt ggttacgggt ttttgttttg cactcttgca cacacatctc
     5701 tcggcctcca aaacaaacac acaactgcac gagggctcga agcccccacc gaaaaacaaa
     5761 aacattatgc taatgaggaa ggcacagctg ttcccttctt cactctctct ctcgttcttt
     5821 caacaacagt cgcctaattt ctctcgttcc tttaaagagc cgccaagcag aaacagttaa
     5881 tgcaaatata agcctgcggg aaataaggag aatttttaca aaatccgagc ttcccctcct
     5941 gccaaaatgt gatgtgtgac tcacgcattc gatatttggt gtaatgagag tgaatcattc
     6001 tgctccacac cgaacccgat gccacagacc actcagatgc acaccgagga gactatttta
     6061 acagcacatt acatccagac tgtcacagat gcattaccaa gagtgtgatt tcagtgtcga
     6121 atgcaggtct taaaggaaaa tctgttgcga ttgcatgtgc gctaactaga cacgaccaac
     6181 gaatgggaac aatctgtgaa atattataca atcacaggca attaaaagat gtttaaagtt
     6241 caatttagtt ttaattaata tgttatataa aactaatctg ttttaaatgt atatttatga
     6301 ttttagcttt agctagcctc ttgaccagca gctgaagtgt ccggcaagta cctaaaaggg
     6361 agaccacatg ggaaagctag gttgctcccg gaagaggtgt tagtaaacca gcaggtctac
     6421 ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtcaacc tgtggtctgt
     6481 gtgggtccta atgccccagt ataaaccagc aggtctacct gtggtctgtg tgggtcctaa
     6541 tgccccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncctg tggtctgtgt
     6661 gggtcctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg
     6721 ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccaggataa
     6781 accagcaggt caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc
     6841 aacctgtggt ctgtgtgggt cctaatgccc cagtatattg atggagactc tatactgctc
     6901 ggtgagctcc atctttcgga taagatgtta aaacaaggtc ccgactctct gtggtggtaa
     6961 aaaattccca ggatgtcctt cctaccctgg catcctggac aaatttgctc actgtcctct
     7021 gtccatcatg gcctcctaac catccccata tcataattgg ctttatcact ctagctgggg
     7081 ccgaaatcgc atacttccat tttatgaggt acgctaaaat cagtatgcga gccaagtaat
     7141 atgtccgaat acttccggcg agattctaga gtgtgcattc gatgcacgct acgctattcc
     7201 atgatgcccc gtgagagaat tcttgaatcg gagagaagca acgcaactca tgcgggtagg
     7261 ttacgtgacg atgacatatt ggcggatgta gtacgtccaa gttccattca tactgctcac
     7321 attcatattt tatagaatgt tcttttccca cagccaagta gtaagtttaa attcaaatac
     7381 agtacctact aagtagtagt agacggtttc ggacgcagcc cctgtctcct ctccaccaat
     7441 atactggtgt gtagtgtgcg gtctggtgca atatggctgc catcgcgtca tccaggtgga
     7501 tgctgcacac tggtggttga tgaggagatt ttgcccaaaa aagtgttttg agtttccaga
     7561 aaagcgcgat ataaatgtaa ggaattatca ttagcttatt ataatggaac tttctggata
     7621 acttgtgcca gagctagggg ccattaaaac tttgccctgg ggtacctcag ggagctgcaa
     7681 ggaagtgcta tagggctaat gaaatgtttg ggggaaaaaa aaaaatttta taagtaaaaa
     7741 taaaactaaa ataattaata atttcattgt tgaaaattat agtaaaattg ataaacaaga
     7801 taagcagcat tagtataaaa aactaaaaac aaacgtaatt taaaataatt aaattgtttt
     7861 aaataaatta tacaaaataa tgtttacaac gacacggtgg tgcagtaggt agtgctgtcg
     7921 ccagcaaagc aagaaggtcg ctggttcgag cctcagctgg atcacttgat gtttctgtgt
     7981 ggagcattcg cgtgcgttta accccagatt aataaagaga ctaagccgaa aagaaaataa
     8041 atgtatgaaa atgtttacgt aaaatgtaaa taaatgcatg attaataaaa tatatgaatt
     8101 ttaaagattg aggtagaatt gttttattat tcacacattt ggttattttt aatcagtgac
     8161 aacttgtgat gctctttata ttgtttatta cgctactttg aatattcagt ctcaaattgc
     8221 atgtaaatac gacttcaaag cagaattagt ttaatttaat tgcctgtaaa cagtgttgta
     8281 ctttgatggt gattgtctga taataatatg atttcactat ttaaaatttg aaaaaaaaaa
     8341 atctgaaaaa tactcaccgg ccactttatt aggtacacct gtctaactgc ttgttaacgt
     8401 aaatttctaa tcagccaatc accaggcagc aactcagtgc atttaggcat gtagacatgg
     8461 tcaagacgat ctgctgcagt tcaaagcgag catcagaatg gagaagcaag gtgatttaag
     8521 tgactttaaa cgtgacgtgt ttgttggtgc cagacgggct ggtctgagta tttcagaaac
     8581 tgctgattta ctggggtttt cacacacaaa catctctggg gtttacagag aatggtccaa
     8641 aaaaagataa tatccagtga gcggcagttc tgtgggggca aatgtcttgt tgatgccaga
     8701 ggtcagagga gaatggcctg actggttcca gctgatagaa aggcaacagt aattcaaata
     8761 accactcgtt agaactgagg catgcagaag agcacctttg aacacaacat gtcgaacctt
     8821 gaggcgggtg ggctacagca gctcaagacc acatcgggtg ccactcctgt cagctaagaa
     8881 caggaaactg aggctacaat tcgcacaggc tcaacataag tagacaatag aagattgaac
     8941 attatgttgc ctggtcagat ggatacattc taccttgtat caacagttca ggctggtgat
     9001 ggtggtgtaa tggtgtgggg gatatattct tggcacactt tgggcccatt agtaccaatt
     9061 aaccatcgtg tcaacgccac agcctaactg agtattgttg ctgaccatgt tcatttcttt
     9121 ttgaccacag tgtacccatc ttctgatggc tacttcctgc aggataatgc accatgtcat
     9181 aaagcgtgaa tgatctcaga ctggtttctt gaacatgaca atgagttcac tgtgctcgaa
     9241 tgacctccac agtcaccaga tctcaatcca atagagcgcc tttggggtgt ggtagaatgg
     9301 aagattcgca tcctggatgt gcagccgaca aatctgcatg atgctagcat gtcaatatgg
     9361 accaaaatct ctgaggaatt ttccagtacc tggttgaaat gcaaaaggaa gggaatctat
     9421 gctaaggcaa aaagaggtcc aacccagtac tagtggggtg tacataataa agtggccggt
     9481 gagtgtatat tgttaaggat atctagataa tccaaataat ttacagcact atttaaaaaa
     9541 aaaactttat gtcaccaagt ctgcatttat ttgatcacaa ttacagtcga aaaagcaata
     9601 tcgggataca ttgtttccat ttaaaataac tatattatat tttaaatatt tttgacaatg
     9661 ttatttcttt aatgccaaag ctaaattgta ttcaatctga attttagtgt cacatgatcc
     9721 ttcagaaaat attaacacat tgctaaaaat gtatattatt atcaatttgg attttttttt
     9781 tttttttgta tattaaccta tgctattggt tactatacaa aagtattact gtgaatatat
     9841 gcatatacag aaccgtatca agcgcggggg gtggttgggt tatgtcccca tttggaaaga
     9901 cctggaaaca ttcataatga ctaaaattgc acggaaaaat ataatatcta tgagtattgc
     9961 tgatcagcag cacaaaacca gaactccata ttaataaaca gccaatgatg cagtgagttt
    10021 gtgcatgttt tcattcaaac tcgtgtgatt gtgctcaaac acctgttgtg agcagcagtt
    10081 gcaaacagta aacctcaaga ggagccagtc aaccttcaca aacactcaac ctctggctca
    10141 ctcactaaac aattcgcgtt aatatctaaa ataaaaattt ccagcaccaa acagctctca
    10201 gatttcggag tacactgacc aagaggtaag aagtaacgtt acattactag gacgactatt
    10261 gtaagactag atatgtaata tttggaagcg tacgtgtttt aagtgacata tagagtagag
    10321 gaactgatgg aaatacgatt tgcataatgt aaaatgatat atattaaaat ataggctaca
    10381 gtaggtaacg tggtttacat ataacgttac tgtgttaatg tagtgctcag ttactcgtgt
    10441 tgacagttat tattagtgct tgctaatttg tttgttaatg cttctcttgc gtatttgatt
    10501 accttcaggc gaatctaaaa catatcagat gtagaaacgg cagaaaaatg ttgaagaaaa
    10561 tacgagaata gtagcttttg gagcacatag aaaagtacat cagataaagc ctaccgttta
    10621 aacgcagacg ccgctctcgc actgaatgct gggtactgta gtccttatat tgtatgctta
    10681 atgtgcgtct caacgggaaa ctagagttag gaaaactaca atttcctgtg gaaaatgttt
    10741 ctgttttcgg ggaaacagcg aaagaaagat atggttccgc gtttttttct tactttgaat
    10801 aataaaataa gacctaacta cacacttcgc ttactcttat gctaatttga agcactagca
    10861 ttctttgata caatgataat catttaaaca tcacagttat actacaaata ctatggttaa
    10921 actatgtcta gtgtagtgta acactatggt tgccattgtt atctttaagt ttaagtatta
    10981 tctgtggtaa agccatggtt ggtttttata agaggtaata aaattaacat tgtacaatta
    11041 aatgactata aattaatgtt tttttttatt ttttttacac agctaccttc aaaaagggat
    11101 ccatgctcaa aagccgactg cggatgttcc tcaatgaatt gaagcttctc gtacttacag
    11161 gaggtggtag accaagagca gaaccacaac ctagaggagg cagaggagga ggatgtggat
    11221 gggcaccttt tgcaggatgt tctacaagag atggagatgg tgatgaagaa gaatattatg
    11281 gatctgaacc tagagcaaga ggattggctg gcgataaaga accaagagca ggacctttgc
    11341 ctccaccagc acctcctttg cctccaccag gagctctcga cgctctgagt ctgtcttcat
    11401 cacttgattc tggactcaga acaccacaat gtcggatctg ctttcaagga cccgaacagg
    11461 gggagttgct ttctccctgt cgctgcgatg gttctgtcag gtgcacgcat cagccttgtc
    11521 tgatacggtg gatttctgaa agaggatctt ggagttgcga gttgtgttat tttaagtatc
    11581 aggtattggc gatctctacc aaaaatccgc tgcaatggca agcgatatct ctgacagtaa
    11641 tcgaaaaggt tcaaatcgcg gcgatcgtgc tggggagctt gtttctggtc gcaagcatct
    11701 cttggctgat ttggagtagc ttgtctccca gcgcaaaatg gcaacggcaa gaccttctct
    11761 ttcagatctg ctatggaatg tacggattta tggatgtagt gtgcatcggg ctgatcatcc
    11821 atgaggggtc ttcagtctac cgcatattta aaagatggca agcagtcaat cagcaatgga
    11881 aagtactcaa ttatgacaaa acaaaagata tcggtggtga cgctggcggg gggaccgcag
    11941 gaaaatcagg acctagaaat tcaagaacag gacctacatc aggagcaaca tcaagacctc
    12001 cagcagcaca acgaatgaga acacttcttc cacaaagatg tggatatact atactccacc
    12061 tcttgggtca gttgcggccg ccagacgcaa gatcttcatc acattctgga agagaggtag
    12121 tgatgcgcgt aacgactgtc tagatcgatg atgatccaga catgataaga tacattgatg
    12181 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
    12241 atgctattgc tttatttgta accattataa g
//
