LOCUS       sequence-460300-       15502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express AGAP2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15502)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 15502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:AGAP2_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..15502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    8488..8622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8643..8777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(9079..9792,9793..9795,9796..9798))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9082..9792,9793..9795,9796..9798))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9124..9145)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9473..9492
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9732..9753
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10979..10995)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10979..10995)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11050..11068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11078..11095)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11078..11094)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11087..11109)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(11235..11690)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11372..11393)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11584..11603
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11717..11821
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11822..11890,11891..12682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12040..12059)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12853..13441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13342..13361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13595..13612
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13729..13750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13765..13770,13771..13788,13789..13795)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13803..13819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13808..13830
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13827..13843
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13827..13843
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13862..13882
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13864..13882
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt
       61 ctgtgtgggt cctaatgccc cagtataaac cagcaggtct acctgtggtc tgtgtgggtc
      121 ctaatgcacc agtataaacc agcaggtcaa cctgtggtct gtgtgggttc taatgcccca
      181 gtataaacca gcaggtcaac ctgtggtctg tgtgggtcca aatgccccag tacaaaccag
      241 caggtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtcaacct
      301 gtggtctgtg tgggtcctaa tgccccggta caaaccagca tgtcaacctg tggtctgtgt
      361 gggtcctaat gccccggtat aaaccagcag atcaacctgt ggtctgtgtg ggtcctaatg
      421 ccccagtata aaccagcaga tcaacctgtg gtctgtgtgg gtcctaaagt cccaggataa
      481 accagcatgt caacctgtgg tctgtgtggg tcctaatgcc ccggtataaa ccagcagatc
      541 aacctgtggt ctgtgtgggt cctaatgccc caggataaac cagcaggtca acctgtggtc
      601 tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc
      661 taatgcccca gtatattgat ggagactcta tactgctcgg tgagctccat ctttcggata
      721 agatgttaaa acaaggtccc gactctctgt ggtggtaaaa aattcccagg atgtccttcc
      781 taccctggca tcctggacaa atttgctcac tgtcctctgt ccatcatggc ctcctaacca
      841 tccccatatc ataattggct ttatcactct agctggggcc gaaatcgcat acttccattt
      901 tatgaggtac gctaaaatca gtatgcgagc caagtaatat gtccgaatac ttccggcgag
      961 attctagagt gtgcattcga tgcacgctac gctattccat gatgccccgt gagagaattc
     1021 ttgaatcgga gagaagcaac gcaactcatg cgggtaggtt acgtgacgat gacatattgg
     1081 cggatgtagt acgtccaagt tccattcata ctgctcacat tcatatttta tagaatgttc
     1141 ttttcccaca gccaagtagt aagtttaaat tcaaatacag tacctactaa gtagtagtag
     1201 acggtttcgg acgcagcccc tgtctcctct ccaccaatat actggtgtgt agtgtgcggt
     1261 ctggtgcaat atggctgcca tcgcgtcatc caggtggatg ctgcacactg gtggttgatg
     1321 aggagatttt gcccaaaaaa gtgttttgag tttccagaaa agcgcgatat aaatgtaagg
     1381 aattatcatt agcttattat aatggaactt tctggataac ttgtgccaga gctaggggcc
     1441 attaaaactt tgccctgggg tacctcaggg agctgcaagg aagtgctata gggctaatga
     1501 aatgtttggg ggaaaaaaaa aaattttata agtaaaaata aaactaaaat aattaataat
     1561 ttcattgttg aaaattatag taaaattgat aaacaagata agcagcatta gtataaaaaa
     1621 ctaaaaacaa acgtaattta aaataattaa attgttttaa ataaattata caaaataatg
     1681 tttacaacga cacggtggtg cagtaggtag tgctgtcgcc agcaaagcaa gaaggtcgct
     1741 ggttcgagcc tcagctggat cacttgatgt ttctgtgtgg agcattcgcg tgcgtttaac
     1801 cccagattaa taaagagact aagccgaaaa gaaaataaat gtatgaaaat gtttacgtaa
     1861 aatgtaaata aatgcatgat taataaaata tatgaatttt aaagattgag gtagaattgt
     1921 tttattattc acacatttgg ttatttttaa tcagtgacaa cttgtgatgc tctttatatt
     1981 gtttattacg ctactttgaa tattcagtct caaattgcat gtaaatacga cttcaaagca
     2041 gaattagttt aatttaattg cctgtaaaca gtgttgtact ttgatggtga ttgtctgata
     2101 ataatatgat ttcactattt aaaatttgaa aaaaaaaaat ctgaaaaata ctcaccggcc
     2161 actttattag gtacacctgt ctaactgctt gttaacgtaa atttctaatc agccaatcac
     2221 caggcagcaa ctcagtgcat ttaggcatgt agacatggtc aagacgatct gctgcagttc
     2281 aaagcgagca tcagaatgga gaagcaaggt gatttaagtg actttaaacg tgacgtgttt
     2341 gttggtgcca gacgggctgg tctgagtatt tcagaaactg ctgatttact ggggttttca
     2401 cacacaaaca tctctggggt ttacagagaa tggtccaaaa aaagataata tccagtgagc
     2461 ggcagttctg tgggggcaaa tgtcttgttg atgccagagg tcagaggaga atggcctgac
     2521 tggttccagc tgatagaaag gcaacagtaa ttcaaataac cactcgttag aactgaggca
     2581 tgcagaagag cacctttgaa cacaacatgt cgaaccttga ggcgggtggg ctacagcagc
     2641 tcaagaccac atcgggtgcc actcctgtca gctaagaaca ggaaactgag gctacaattc
     2701 gcacaggctc aacataagta gacaatagaa gattgaacat tatgttgcct ggtcagatgg
     2761 atacattcta ccttgtatca acagttcagg ctggtgatgg tggtgtaatg gtgtggggga
     2821 tatattcttg gcacactttg ggcccattag taccaattaa ccatcgtgtc aacgccacag
     2881 cctaactgag tattgttgct gaccatgttc atttcttttt gaccacagtg tacccatctt
     2941 ctgatggcta cttcctgcag gataatgcac catgtcataa agcgtgaatg atctcagact
     3001 ggtttcttga acatgacaat gagttcactg tgctcgaatg acctccacag tcaccagatc
     3061 tcaatccaat agagcgcctt tggggtgtgg tagaatggaa gattcgcatc ctggatgtgc
     3121 agccgacaaa tctgcatgat gctagcatgt caatatggac caaaatctct gaggaatttt
     3181 ccagtacctg gttgaaatgc aaaaggaagg gaatctatgc taaggcaaaa agaggtccaa
     3241 cccagtacta gtggggtgta cataataaag tggccggtga gtgtatattg ttaaggatat
     3301 ctagataatc caaataattt acagcactat ttaaaaaaaa aactttatgt caccaagtct
     3361 gcatttattt gatcacaatt acagtcgaaa aagcaatatc gggatacatt gtttccattt
     3421 aaaataacta tattatattt taaatatttt tgacaatgtt atttctttaa tgccaaagct
     3481 aaattgtatt caatctgaat tttagtgtca catgatcctt cagaaaatat taacacattg
     3541 ctaaaaatgt atattattat caatttggat tttttttttt ttttgtatat taacctatgc
     3601 tattggttac tatacaaaag tattactgtg aatatatgca tatacagaac cgtatcaagc
     3661 gcggggggtg gttgggttat gtccccattt ggaaagacct ggaaacattc ataatgacta
     3721 aaattgcacg gaaaaatata atatctatga gtattgctga tcagcagcac aaaaccagaa
     3781 ctccatatta ataaacagcc aatgatgcag tgagtttgtg catgttttca ttcaaactcg
     3841 tgtgattgtg ctcaaacacc tgttgtgagc agcagttgca aacagtaaac ctcaagagga
     3901 gccagtcaac cttcacaaac actcaacctc tggctcactc actaaacaat tcgcgttaat
     3961 atctaaaata aaaatttcca gcaccaaaca gctctcagat ttcggagtac actgaccaag
     4021 aggtaagaag taacgttaca ttactaggac gactattgta agactagata tgtaatattt
     4081 ggaagcgtac gtgttttaag tgacatatag agtagaggaa ctgatggaaa tacgatttgc
     4141 ataatgtaaa atgatatata ttaaaatata ggctacagta ggtaacgtgg tttacatata
     4201 acgttactgt gttaatgtag tgctcagtta ctcgtgttga cagttattat tagtgcttgc
     4261 taatttgttt gttaatgctt ctcttgcgta tttgattacc ttcaggcgaa tctaaaacat
     4321 atcagatgta gaaacggcag aaaaatgttg aagaaaatac gagaatagta gcttttggag
     4381 cacatagaaa agtacatcag ataaagccta ccgtttaaac gcagacgccg ctctcgcact
     4441 gaatgctggg tactgtagtc cttatattgt atgcttaatg tgcgtctcaa cgggaaacta
     4501 gagttaggaa aactacaatt tcctgtggaa aatgtttctg ttttcgggga aacagcgaaa
     4561 gaaagatatg gttccgcgtt tttttcttac tttgaataat aaaataagac ctaactacac
     4621 acttcgctta ctcttatgct aatttgaagc actagcattc tttgatacaa tgataatcat
     4681 ttaaacatca cagttatact acaaatacta tggttaaact atgtctagtg tagtgtaaca
     4741 ctatggttgc cattgttatc tttaagttta agtattatct gtggtaaagc catggttggt
     4801 ttttataaga ggtaataaaa ttaacattgt acaattaaat gactataaat taatgttttt
     4861 ttttattttt tttacacagc taccttcaaa aagggatcca tgagccgggg cgcgggcgcg
     4921 cttcagcgcc ggacaacgac ctacctcatc tcgctgaccc tggttaagct cgagtcggtg
     4981 cctccgccgc cgccttctcc gtctgcggcc gcggccggcg ccgccggtgc cagaggctcc
     5041 gagactgggg accctggcag cccccgaggc gcggaggagc cgggcaagaa gcggcacgaa
     5101 cgtctcttcc accggcagga tgcgctgtgg atcagcacga gcagcgcggg caccgggggc
     5161 gcggagcccc cagccctgtc cccggctccg gccagtccgg cccgcccagt ctcccccgct
     5221 cccggccgcc gcctctccct ctgggccgtc cctccgggac ccccgctctc cgggggactg
     5281 agccccgacc ccaagcctgg gggcgccccc acctcctccc ggcgccccct gctcagcagc
     5341 ccgagctggg gcggcccgga gcccgaaggc cgggcgggcg gcggcatccc tggctcatcc
     5401 tctccgcacc ctggcaccgg cagccggagg ctcaaggtgg cgcctcctcc gccggctccc
     5461 aagccttgca agaccgtgac cacgagtgga gccaaagccg gcgggggcaa gggcgcgggt
     5521 agccgcctgt catggcccga aagcgagggc aagcccaggg tcaaggggtc aaagagcagc
     5581 gccgggactg gagcttcggt ctctgccgcc gccaccgccg ccgccgccgg gggagggggc
     5641 tctacagctt cgacctctgg tggggtcggg gctggggctg gagcccgagg gaagttgtcc
     5701 cctcggaaag gcaagagtaa gaccttggac aacagtgact tgcatccggg accgcctgcc
     5761 ggctctcctc ctccgctaac cctcccacca actccgagtc cagccactgc tgtcaccgct
     5821 gcttccgcgc agccccccgg gcctgcacct ccaatcactc tggagcctcc agctccgggg
     5881 ctgaaacggg gccgggaggg gggccgagca tccactcgtg accgcaagat gctcaagttt
     5941 atcagcggca tcttcaccaa gagcacagga gggcctcctg gctccgggcc ccttcccgga
     6001 ccccccagcc tgtcttctgg cagcgggtcc agggagctgc tgggcgccga gctccgcgct
     6061 tcccctaagg ctgtgatcaa tagccaggaa tggactttga gccgctccat tcctgaactg
     6121 cgcctgggtg tgctgggcga tgccaggagt gggaagtcat cgctcatcca ccgattcctg
     6181 actggctcat accaggtgct ggagaagaca gagagtgagc agtacaagaa agaaatgttg
     6241 gtggatggac agacacatct ggtgctaatc cgagaggaag ctggggcacc tgatgccaag
     6301 ttctcaggct gggcagatgc tgtgatcttc gtcttcagcc tggaggatga gaacagtttc
     6361 caggctgtga gccgtctcca tgggcagctg agttcccttc gcggggaggg acgaggaggc
     6421 ctggccttgg cactggtggg gacacaagac aggatcagtg cttcctcccc tcgggtggtg
     6481 ggagatgctc gtgccagagc tctgtgcgcg gacatgaaac gctgcagcta ctatgagact
     6541 tgtgcaacct atgggctcaa tgtggatcgg gtcttccagg aggtggccca gaaggtggtg
     6601 accttgcgca agcagcaaca gcttctggct gcctgcaagt ccctgcccag ctccccaagc
     6661 cactcagctg catccactcc ggtagctggc caggctagta acgggggcca cactagcgac
     6721 tactcttctt ccctcccgtc ctcaccgaat gttggtcacc gggagctccg agccgaggca
     6781 gctgcagtgg ctggattgag caccccaggg tccctgcacc gggcagccaa gcgcaggacc
     6841 agcctttttg cgaatcgtcg gggtagtgac tccgagaaac gaagcttgga tagtcgggga
     6901 gagacaacag ggagtgggcg agccatcccc atcaaacaga gcttcctact aaaacgaagt
     6961 ggcaattcct tgaacaaaga atggaagaag aaatatgtaa ccctgtccag taatggcttt
     7021 ctactctacc accccagtat taacgattac atccacagta cccacggcaa ggagatggac
     7081 ttgctgcgaa caacagtcaa agtcccgggc aagcggcccc cgagggccat ctctgccttt
     7141 ggcccctcag ccagcattaa cgggctcgtc aaggacatga gcactgtcca gatgggtgaa
     7201 ggcctggaag ccactactcc catgccaagc cctagcccca gccccagttc cctgcagcca
     7261 ccaccagatc agacatccaa acacctgctg aagccagacc ggaatttggc ccgagccctc
     7321 agcacggact gtaccccatc tggagacctg agccccctga gtcgggaacc ccctccttct
     7381 cccatggtga agaagcagag gaggaaaaaa ttgacaacac catccaagac tgaaggctcg
     7441 gctgggcagg ctgaagccaa gcgcaaaatg tggaaactaa aatcctttgg tagtttaaga
     7501 aatatttata aagcagagga aaactttgag ttcctgatcg tgtccagcac gggtcagacg
     7561 tggcactttg aggcagccag ttttgaggag cgggatgcct gggtccaggc catcgagagt
     7621 cagatcctag ccagtctgca atgctgtgag agcagcaagg tcaagctgcg cacagacagc
     7681 caaagcgagg ccgtggccat ccaggcgatc cggaacgcca aggggaattc aatctgcgtg
     7741 gactgcgggg cccccaaccc cacgtgggcc agcttgaacc tgggcgccct catctgcatc
     7801 gagtgttctg gcatccaccg caacctgggc acacacctgt cccgcgttcg ctcgctggac
     7861 ttggacgact ggccacggga gctgaccctg gtgctgacgg ctattggcaa cgacacggcc
     7921 aaccgcgtgt gggaaagcga cacgcgaggc cgtgccaagc cctcgcggga ctcttcgcgg
     7981 gaggagcgcg agtcgtggat tcgcgccaag tacgagcagc tactgttcct ggcgccgctg
     8041 agcacctcgg aggagccgct gggccgccag ctgtgggccg ccgtgcaggc ccaggacgtg
     8101 gctaccgttc tcctgctttt ggcccatgcg cgacacgggc cgctcgacac cagcgtagag
     8161 gacccacagc tgcgctcccc actccacctg gcggccgagc tcgcccacgt cgtcatcacg
     8221 caactgctgc tgtggtacgg cgcggacgtg gcggcccgtg acgcccaggg ccgcacggcg
     8281 ctgttctacg cccgccaggc tggaagccag ctgtgcgccg acatccttct ccagcacggc
     8341 tgcccgggtg agggcggcag cgcggccacc acgcccagcg cggccaccac gcccagcatc
     8401 accgccacgc ccagcccccg ccgccggagc agcgccgcta gcgtgggccg cgccgacgcc
     8461 ccggttgcgc tggtatagat cgatgatgat ccagacatga taagatacat tgatgagttt
     8521 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     8581 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattcgatg
     8641 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     8701 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     8761 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     8821 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     8881 atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag
     8941 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9001 actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga
     9061 ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     9121 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     9181 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     9241 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     9301 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     9361 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     9421 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     9481 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     9541 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     9601 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     9661 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     9721 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     9781 ttcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac
     9841 tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt
     9901 ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc
     9961 tgataactgc acaagtgctc agattccagc agggtggaaa tgagataaag tgtgcagatg
    10021 gggaggggga cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa
    10081 tagccccttt cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac
    10141 ctgtaaatga tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca
    10201 aattcttaat tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg
    10261 aattttctgg aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac
    10321 atggaatgaa gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa
    10381 ataagagtga aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt
    10441 gtacaaacag tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg
    10501 cttggatgta tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct
    10561 aaagttacaa ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg
    10621 ttaagtctgc tgatttggag agctcattta catttatttt gaatgtctct tatttaacac
    10681 aactgattta agctttagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
    10741 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
    10801 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
    10861 agatgtgaca tgattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
    10921 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
    10981 tccactagtt ctagagcggc cattaccctg ttatccctag gccgccaccg cggtggagct
    11041 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    11101 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    11161 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    11221 tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    11281 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    11341 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    11401 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    11461 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    11521 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    11581 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    11641 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
    11701 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    11761 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    11821 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    11881 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    11941 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    12001 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    12061 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    12121 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    12181 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    12241 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    12301 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    12361 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    12421 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    12481 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    12541 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    12601 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    12661 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    12721 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    12781 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12841 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    12901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    12961 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    13021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    13081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    13141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    13201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    13261 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    13321 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    13381 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    13441 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    13501 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    13561 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    13621 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    13681 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    13741 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    13801 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    13861 cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaattacc
    13921 ctgttatccc tatcgagcaa aatcaatcct aatgtaaaat agtatacgtt catcattata
    13981 atgcaaacac tgtagaactg ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt
    14041 ctgtctcttt ttacgacaga gctagaaaaa actagccttc atttgccatc gccgtatgca
    14101 aatccactcc agaactcaac aggtttgttc tgcggcgggt gtaagtgtga acaccggcat
    14161 gtaagtgcgc ttgagcagga gaataaagcg ctcagagaca ccggaggaat cctttatttt
    14221 gcgttgcgcc tcaggggatt cgctgagcct tctcttcatg gccctgctct tcagacacag
    14281 gtgctccgct gtttgtgttt ttcactgttc ttcaggcact cgtggttacg ggtttttgtt
    14341 ttgcactctt gcacacacat ctctcggcct ccaaaacaaa cacacaactg cacgagggct
    14401 cgaagccccc accgaaaaac aaaaacatta tgctaatgag gaaggcacag ctgttccctt
    14461 cttcactctc tctctcgttc tttcaacaac agtcgcctaa tttctctcgt tcctttaaag
    14521 agccgccaag cagaaacagt taatgcaaat ataagcctgc gggaaataag gagaattttt
    14581 acaaaatccg agcttcccct cctgccaaaa tgtgatgtgt gactcacgca ttcgatattt
    14641 ggtgtaatga gagtgaatca ttctgctcca caccgaaccc gatgccacag accactcaga
    14701 tgcacaccga ggagactatt ttaacagcac attacatcca gactgtcaca gatgcattac
    14761 caagagtgtg atttcagtgt cgaatgcagg tcttaaagga aaatctgttg cgattgcatg
    14821 tgcgctaact agacacgacc aacgaatggg aacaatctgt gaaatattat acaatcacag
    14881 gcaattaaaa gatgtttaaa gttcaattta gttttaatta atatgttata taaaactaat
    14941 ctgttttaaa tgtatattta tgattttagc tttagctagc ctcttgacca gcagctgaag
    15001 tgtccggcaa gtacctaaaa gggagaccac atgggaaagc taggttgctc ccggaagagg
    15061 tgttagtaaa ccagcaggtc tacctgtggt ctgtgtgggt cctaatgccc cagtataaac
    15121 cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcta
    15181 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtagtctg
    15241 tgtgggtcct aatgccccag tataaaccag caggtctacc tgtggtctgt gtgggtccta
    15301 atgccccagt ataaaccaac aggtcaacct gtgatctgtg tgggtcctaa tgccccagta
    15361 taaaccagca ggtcaacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
    15421 gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg
    15481 gtctgtgtgg gtcctaatgc cc
//
