LOCUS       sequence-460302-        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral overexpression vector for NADK (D184A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Flickinger KM, Mellado Fritz CA, Huggler KS, Wade GM, Chang GR, Fox 
            KC, Simcox JA, Cantor JR
  TITLE     Cytosolic NADK is conditionally essential for folate-dependent 
            nucleotide synthesis.
  JOURNAL   Nat Metab. 2025 May 2. doi: 10.1038/s42255-025-01272-3.
  PUBMED    40316835
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2025 May 2. doi: 10.1038/s42255-025-01272-3."
COMMENT     Sequence Label: pLJC2-NADK (D184A)-3xFLAG
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1360..1425
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggagatgg agcaggagaa aatgacgatg aataaagagc ttagcccaga cgctgccgca
       61 tattgctgtt cagcgtgcca tggtgacgaa acatggagct ataaccatcc tatacgaggc
      121 agagcaaaga gcagaagctt gtctgcgtcc ccagccctcg ggtctactaa ggaattccgc
      181 cgaactagaa gtctccacgg gccttgtcct gtgaccacat ttgggcccaa ggcatgtgtt
      241 cttcagaatc cgcagacaat catgcacatt caggatccag cttcccagag gctcacgtgg
      301 aataagagtc caaagtccgt tctcgtaatc aaaaaaatgc gggatgcgtc actcctccaa
      361 ccgttcaaag agctttgcac acacttgatg gaagaaaata tgatcgttta cgtcgaaaag
      421 aaagtactcg aggacccggc gatcgcttct gatgaaagtt ttggcgctgt gaaaaaaaaa
      481 ttttgtacgt ttcgggaaga ttacgacgac atatccaatc agatcgactt tataatctgt
      541 ttgggagggg ccgggacgct tctgtatgca tcctcccttt ttcagggcag cgttccacct
      601 gtcatggcat tccatctcgg ctctcttggt tttctcacgc cattctcttt tgagaacttt
      661 caatctcagg taacgcaggt catagagggt aacgccgccg ttgttttgcg ctcccgcctg
      721 aaagtcaggg ttgtaaagga actgcgaggg aagaaaacag ccgtccataa cggtctcgga
      781 gaaaacggga gtcaagcagc cggcctggac atggatgtag gcaaacaggc tatgcaatat
      841 caagtcctga acgaagttgt tatcgaccgc ggcccgagtt cctacctcag taacgtagac
      901 gtatatctgg acggtcattt gattaccacg gtccaaggtg atggggttat cgtgagcaca
      961 cccaccgggt ccacagccta tgcagccgct gcaggtgcgt caatgatcca cccaaacgtg
     1021 ccggccataa tgatcacacc gatttgtcct cactcactct ctttccgacc gatagtagta
     1081 ccggccggag tagagttgaa aattatgctg tcccctgaag cgcggaatac cgcttgggtg
     1141 tccttcgatg ggagaaaacg gcaggaaatc cggcacgggg attccataag tatcaccact
     1201 tcctgctatc ctctgccgag catttgtgtg cgcgacccgg tatcagattg gtttgaatcc
     1261 ctggctcaat gtcttcattg gaacgttcgc aagaagcagg ctcattttga ggaggaagag
     1321 gaagaagaag aggagggcgg aggcagcgcg gccgcaggcg actacaaaga ccatgacggt
     1381 gattataaag atcatgacat tgattacaag gatgacgatg acaagtaata a
//
