LOCUS       sequence-460321-        9935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minigene of mouse MAPT containing exon 9, 10, 11 and relevant 
            intronic sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Recinos Y, Bao S, Wang X, Phillips BL, Yeh YT, Weyn-Vanhentenryck 
            SM, Swanson MS, Zhang C
  TITLE     Lineage-specific splicing regulation of MAPT gene in the primate 
            brain.
  JOURNAL   Cell Genom. 2024 Jun 12;4(6):100563. doi: 
            10.1016/j.xgen.2024.100563. Epub 2024 May 20.
  PUBMED    38772368
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2024-06-12"; volume: "4(6):100563. doi"; pages: " 
            10.1016/j.xgen.2024.100563. Epub 2024 May 20"
COMMENT     Sequence Label: pcDNA5/FRT-mMAPT
FEATURES             Location/Qualifiers
     source          1..9935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             645..656
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(2167..2184)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2173..2397
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2530..2549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    2681..2728
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2736..3758
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             2736..3758
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    3888..4009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3925..3944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3979..3998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4058..4074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4058..4074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4071..4093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4082..4098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4106..4112,4113..4130,4131..4136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4151..4172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4289..4306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4460..5048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4540..4559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5219..6010,6011..6079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5842..5861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6080..6184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6259..6278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6450..6829
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6830..7033
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6984..7004
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7078..7097
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7078..7096
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcctctgtc ctccactccc cgctggccga tcctgccacc tcccttactt gttttgcttc
       61 ctaaggaaag gcggggctgt tggctacact gcggttactg ccctgcctgc cggttgcctt
      121 ctcaacgtat agaaagatat gggttgcggg aaagggtgat ctttaagatc gccgtccttg
      181 tccctgggct ctgtaatgtt gtttatgata cgttagcttc ccagcccagg ctgctgtggt
      241 tagggaggga agccagaagt gtctggtttt gctcacgtct ggtctgttgc tgggtctgag
      301 cgctccagct cccaagggct gcagagggca gatgctcctg taggggaagt tgggaaaagg
      361 cagctctcag ggcagctcgg atcacatgct ggttgtctga tgaggctgca gtgcctaggg
      421 tatcagggaa ctttataact aaggtgtact gcagtttgcc agcctttgag ctaacctgtg
      481 accttatata gaatcacaag atagcctggc ctgtggcctg tggccactgt tgactacaga
      541 atggacctgt ggagaaagct ttcttaggga gggggactgg tggcatttgg gttgtaggat
      601 tccaggcttc ccatctaact tgctcctggc tctctctagg gtggtgggaa ctgcagcagg
      661 gaagctgctt ctccatctcc acctcctggc tgggctgtgg cctcctctct cgggctgctc
      721 tgtgggatct agtaccctag tcagctgctc ccccattcct ttcttctctg gctatcccaa
      781 gcttccaaca tctcctgtcc cccaacatac cccctggtac tgactctcta atgactctac
      841 ctgggctctc ccatcatcac ctgccccaga actcctactg gcctctgggt ctgtttgcag
      901 gaggtccttg ggtttatctg ctccttaccc accctcctct gcaggggcag gctgacaccc
      961 ttatgactta tgaggccatg gccctcctct tccatcaggt ctgttagcag aacacacaca
     1021 cacacacaca cacaccccat aacacaaaac accatcagcc gtgaaaacga gggatttatc
     1081 cattgagacc tccgtagaaa ccactttgct ttattgattc ttgaacatgt ggcacccctc
     1141 tcccaggaca gatgagcctc ctcacccctg gaatgaccat ggtctgtatg ggagacccat
     1201 gggactctgc aagtggcagc tagtgaccag ctaagctgta aaaccacaga tcagagagac
     1261 aggcttccag gagccccatc tgcctgggtg gtctccttca gagtgtcctg tcaggaaagc
     1321 aactgtcatt ttccagagca cacattactg ctgggccaca tgaagggccc ttgggctggc
     1381 gggtcccccc cacccccctt ccccagtgtg tcctgtctgc tcactcatgc ttgctgagac
     1441 acttactgca gctgtggttt ctgccgtgcg cctgccttct gttggtgttg cttcgctctt
     1501 ctcagtagag gctgaagtgt tcaggagact atgtgtccag acaccaactg gctctccttc
     1561 tgggtcatag agctggtctg ggtcctgttt gccgactggt aatccaggac catgggagct
     1621 tcctgggtga agcagactca ccctcaccag cttctcctga gtctttttct gaggagaaga
     1681 tgcagggctg tgaaggcttc ttcctccact gggtgtcatt ggcctttcat caataaaacg
     1741 atgtatactt ttatatttaa gtgtctgcta cagagatggt cacgtacaag gaccaggcac
     1801 ctgcttttcc atgagagtgt cttgtgtttc tgggatgttt aggtccctaa gcttcctggt
     1861 gaggacccag ctccctgggg ggtggggggt ggctggattt ggggagccta ttttccatcc
     1921 tgagtggggg tgtatgcagc agctgatggg agccactgac aaatggtcca tcagcctttg
     1981 tgctgtgtta cacccctagt aacataaccc cctttattct ctctctcgcc catcctcttt
     2041 gacctgcagg tgcaaatagt ctacaagccg gtggacctga gcaaagtgac ctccaagtgt
     2101 ggctcgttag ggaacatcca tcacaagcca gtgagtctag agggcccgtt taaacccgct
     2161 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2221 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2281 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2341 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     2401 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     2461 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     2521 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     2581 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2641 accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt
     2701 cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt
     2761 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     2821 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     2881 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     2941 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     3001 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     3061 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     3121 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     3181 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     3241 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     3301 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3361 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3421 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3481 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     3541 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     3601 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     3661 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     3721 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt
     3781 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     3841 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     3901 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3961 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     4021 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4081 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4141 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4201 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4261 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4381 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4621 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4861 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6181 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     6241 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     6301 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     6361 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     6421 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     6481 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6541 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6601 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6661 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6721 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6781 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     6841 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6901 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6961 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     7021 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     7081 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttagtga accaccaaaa
     7141 tccggagaac gaagcggcta cagcagcccc ggctctcccg gaacgcctgg cagtcgctcg
     7201 cgcaccccat ccctaccaac accgcccacc cgggagccca agaaggtggc agtggtccgc
     7261 actcccccta agtcaccatc agctagtaag agccgcctgc agactgcccc tgtgcccatg
     7321 ccagacctaa agaatgtcag gtcgaagatt ggctctactg agaacctgaa gcaccagcca
     7381 ggaggtggca aggtaagggt gtggctggtc ttctgggaag ccagactccc acctagccag
     7441 acttgctccc ccccaccctg ccaccacacc cctccaaccc cggaagcaca cgggccacca
     7501 aggcactggc tcctcccttc ccctctttag ccttgctaat tgggcaggtg acagccactt
     7561 gtctacaggg acccacctat ggtgacctat ggattttgct aagtaccgcc cttgttgggc
     7621 ttggctttca cccttggttc tggggaaatt catggtttta atatgagcag ctggttctac
     7681 ggcagtcatg aaatttgtag acctcttgtc cccaggctcg tcgaggctcc tccttcctcg
     7741 ctttctcctg agagttgctc aggctacata tactcagggt tctggaatct gcaactctga
     7801 acctcagtgt tcccgagcgc tttggcttaa catgaccact agcagtaatt caagtctgct
     7861 ttgtaaatat ctatctagtg ccaaacacca caaaagcttg tttcctttcc tgtggggtta
     7921 gaataagaaa cacacacaca cagggcgggg tctctatgta tgtttcagac cgtgtgcgat
     7981 tgcttaatgt cctctgatta atttgtgtct agcagtcatc ttcctccaaa aaccattttc
     8041 tttgtcaaat catgagttat gtaatcagtc acatttcctt tttcctcatt ggcttccggg
     8101 aagctcggtg ctgaatataa tagtgccctc ttcctcttat tttattgtta ttattgttgt
     8161 tgttattatt gttgttatta ttttgggttt tcaagacagg atttctgtaa attcaaggct
     8221 agcctggtct actactgaat ttcagcatag ctagggctac atagagagac cccgcctcaa
     8281 aatagcatta aaaaaaaaag ttaaaattaa cagaggatag tctaaagtaa ctaagtattg
     8341 tatgtgcacg ccactgtgtt cagaaggcag aagtggggtt ttatgacaag ttctttaccc
     8401 tccctgagcc atcccaccag ctcagctcaa gacaatctgg tttgaagcct ttagccttgc
     8461 cactccccag ccttggacgc tctttgtgat aaggtgcctt gcactggtgt gtctgtttgg
     8521 attgttgttg atctttcttc tcatcacttg acccagggga ctccttgttt acagttgtca
     8581 ctccacccaa cctagtgacc tcccagctct gtcctcctgt tcccagccat gttctcttcc
     8641 agattccttt tgtgacttcc agggtgccat cccaggcagc ttctgagccc cctgaggagt
     8701 cagggctcag acctggctct ccccccacag cccaacaggt cagggggcga tgctccggtg
     8761 tggttgtctc tcactctttt ttttggctat caaaggtgca gataattaat aagaagctgg
     8821 atcttagcaa cgtccagtcc aagtgtggct cgaaggataa tatcaaacac gtcccgggtg
     8881 gaggcagtgt gagtactgtc gcagtctcca tgaggtgtgc tgcagccttt gctgtaacaa
     8941 gtgtcatgag tgtgtccttg tgagacattg catagaataa atcatctagg gctcaggacc
     9001 tcctgtgtcc ccacagtgtc tgctgtcccc agactcccct ccctgttcca tggatggccc
     9061 agcaaagtag ggagagcaac ccgttccagc tggcccttct ctgagtagct gggtccccat
     9121 ggtaccatgt atgtgttcct agaagccccg tagatattgg aaatggggtt gaagacagtg
     9181 catccatgat gtgggctgac acctcttccc tcatctctgg ggcagtctct gttggttggg
     9241 gagtggggag tccatatgat cccactagac actaggactt cctgtttcat gtcaaggtcc
     9301 aaaaggcccc tagccacttc tccatttttc atctgggtct ccttcatctt tgaagagagg
     9361 aaaagattcc cagttcaatt tagcactccc ctccccccag gacagggact ctggttgcac
     9421 agagcctaac accgaggtga caggtgtcag ttgcctgcat tgtttgccag agaaggggtt
     9481 ttaaatgtct cctcctttaa acaccactgg ggaccccccc cccccccgca catcctcagc
     9541 gagtccctgc aaagagatgt tcttaggttc cctcaatggg cttagggatt ccctttgttc
     9601 tcttggctgg ccggtgccca acagggcagg atttgtaaag tggccccagg cttctccctg
     9661 ccagctcccc ctcccctgtt ctgggtcagt cccagaacgg acctgggctc tctgattgag
     9721 ctgcaggctc cacctctcga gcgtgcgtgc tggaatgcag gggtgggctc tctttcctga
     9781 aagtgccgag cccacagggt ttattcttgc aatgtgctcc tgcctccggc cccgacacct
     9841 gccctgcctt ctgcctcttg gaccgatggc cccagagcag caagtgctgg ccctgtggct
     9901 actgccttct cacgcttact caccgtgtct gcctt
//
