LOCUS       sequence-460329-       13144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of PIF6 and PhyB:VP16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 13144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4003
FEATURES             Location/Qualifiers
     source          1..13144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1973..1993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2020..2272
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3116..3460
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5688..5921
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             5922..5942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5969..6221
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6463..6487
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7788..8417
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8846..9919
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9985..10179
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10415..10437)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10523..10663
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10578..10597
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10678..10695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10849..11437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10929..10948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11525..12316)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13102..13126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgacaa caatgccccg ccccaagctc aagtccgatg acgaggtact
     1081 cgaggccgcc accgtagtgc tgaagcgttg cggtcccata gagttcacgc tcagcggagt
     1141 agcaaaggag gtggggctct cccgcgcagc gttaatccag cgcttcacca accgcgatac
     1201 gctgctggtg aggatgatgg agcgcggcgt cgagcaggtg cggcattacc tgaatgcgat
     1261 accgataggc gcagggccgc aagggctctg ggaatttttg caggtgctcg ttcggagcat
     1321 gaacactcgc aacgacttct cggtgaacta tctcatctcc tggtacgagc tccaggtgcc
     1381 ggagctacgc acgcttgcga tccagcggaa ccgcgcggtg gtggagggga tccgcaagcg
     1441 actgccccca ggtgctcctg cggcagctga gttgctcctg cactcggtca tcgctggcgc
     1501 gacgatgcag tgggccgtcg atccggatgg tgagctagct gatcatgtgc tggctcagat
     1561 cgctgccatc ctgtgtttaa tgtttcccga acacgacgat ttccaactcc tccaggcaca
     1621 tgcgtccgcg tacagcttaa ttaacagtgc tggtagtgct ggtagtgctg gtatgatgtt
     1681 cttaccaacc gattattgtt gcaggttaag cgatcaagag tatatggagc ttttgtttga
     1741 gaatggccag attcttgcaa agggccaaag atccaacgtt tctctgcata atcaacgtac
     1801 caaatcgatc atggatttgt atgaggcaga gtataacgag gatttcatga agagtatcat
     1861 ccatggtggt ggtggtgcca tcacaaatct cggggacacg caggttgttc cacaaagtca
     1921 tgttgctgct gcccatgaaa caaacatgtt ggaaagcaat aaacatgttg acccaaagaa
     1981 gaagaggaag gtgtaggctt ggaatggatc ttcgatcccg atcgttcaaa catttggcaa
     2041 taaagtttct taagattgaa tcctgttgcc ggtcttgcga cgattatcat ataatttctg
     2101 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2161 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2221 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     2281 ccaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2341 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     2401 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2461 ctgataaatg cttcaataat gggaccgact cgcgctgtca ggagactaga gccaagctga
     2521 tctcctttgc cccggagatc accatggacg actttctcta tctctacgat ctaggaagaa
     2581 agttcgacgg agaaggtgac gataccatgt tcaccaccga taatgagaag attagcctct
     2641 tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgcg gcaggtctga
     2701 tcaagacgat ctacccgagt aataatctcc aggagatcaa ataccttccc aagaaggtta
     2761 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
     2821 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc
     2881 aagtaataga gattggagtc tctaagaaag tagttcctac tgaatcaaag gccatggagt
     2941 caaaaattca gatcgaggat ctaacagaac tcgccgtgaa gactggcgaa cagttcatac
     3001 agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
     3061 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     3121 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     3181 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     3241 aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg tcccaaagat ggacccccac
     3301 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     3361 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     3421 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat
     3481 taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc gaaatgacaa
     3541 caatggtttc cggagtcggg ggtagtggcg gtggccgtgg cggtggccgt ggcggagaag
     3601 aagaaccgtc gtcaagtcac actcctaata accgaagagg aggagaacaa gctcaatcgt
     3661 cgggaacgaa atctctcaga ccaagaagca acactgaatc aatgagcaaa gcaattcaac
     3721 agtacaccgt cgacgcaaga ctccacgccg ttttcgaaca atccggcgaa tcagggaaat
     3781 cattcgacta ctcacaatca ctcaaaacga cgacgtacgg ttcctctgta cctgagcaac
     3841 agatcacagc ttatctctct cgaatccagc gaggtggtta cattcagcct ttcggatgta
     3901 tgatcgccgt cgatgaatcc agtttccgga tcatcggtta cagtgaaaac gccagagaaa
     3961 tgttagggat tatgcctcaa tctgttccta ctcttgagaa acctgagatt ctagctatgg
     4021 gaactgatgt gagatctttg ttcacttctt cgagctcgat tctactcgag cgtgctttcg
     4081 ttgctcgaga gattaccttg ttaaatccgg tttggatcca ttccaagaat actggtaaac
     4141 cgttttacgc cattcttcat aggattgatg ttggtgttgt tattgattta gagccagcta
     4201 gaactgaaga tcctgcgctt tctattgctg gtgctgttca atcgcagaaa ctcgcggttc
     4261 gtgcgatttc tcagttacag gctcttcctg gtggagatat taagcttttg tgtgacactg
     4321 tcgtggaaag tgtgagggac ttgactggtt atgatcgtgt tatggtttat aagtttcatg
     4381 aagatgagca tggagaagtt gtagctgaga gtaaacgaga tgatttagag ccttatattg
     4441 gactgcatta tcctgctact gatattcctc aagcgtcaag gttcttgttt aagcagaacc
     4501 gtgtccgaat gatagtagat tgcaatgcca cacctgttct tgtggtccag gacgataggc
     4561 taactcagtc tatgtgcttg gttggttcta ctcttagggc tcctcatggt tgtcactctc
     4621 agtatatggc taacatggga tctattgcgt ctttagcaat ggcggttata atcaatggaa
     4681 atgaagatga tgggagcaat gtagctagtg gaagaagctc gatgaggctt tggggtttgg
     4741 ttgtttgcca tcacacttct tctcgctgca taccgtttcc gctaaggtat gcttgtgagt
     4801 ttttgatgca ggctttcggt ttacagttaa acatggaatt gcagttagct ttgcaaatgt
     4861 cagagaaacg cgttttgaga acgcagacac tgttatgtga tatgcttctg cgtgactcgc
     4921 ctgctggaat tgttacacag agtcccagta tcatggactt agtgaaatgt gacggtgcag
     4981 catttcttta ccacgggaag tattacccgt tgggtgttgc tcctagtgaa gttcagataa
     5041 aagatgttgt ggagtggttg cttgcgaatc atgcggattc aaccggatta agcactgata
     5101 gtttaggcga tgcggggtat cccggtgcag ctgcgttagg ggatgctgtg tgcggtatgg
     5161 cagttgcata tatcacaaaa agagactttc ttttttggtt tcgatctcac actgcgaaag
     5221 aaatcaaatg gggaggcgct aagcatcatc cggaggataa agatgatggg caacgaatgc
     5281 atcctcgttc gtcctttcag gcttttcttg aagttgttaa gagccggagt cagccatggg
     5341 aaactgcgga aatggatgcg attcactcgc tccagcttat tctgagagac tcttttaaag
     5401 aatctgaggc ggctatgaac tctaaagttg tggatggtgt ggttcagcca tgtagggata
     5461 tggcggggga acaggggatt gatgagttag gtgaattcga tagtgctggt agtgctggta
     5521 gtgctggttc gggttccgcg tacagccgcg cgcgtacgaa aaacaattac gggtctacca
     5581 tcgagggcct gctcgatctc ccggacgacg acgcccccga agaggcgggg ctggcggctc
     5641 cgcgcctgtc ctttctcccc gcgggacaca cgcgcagact gtcgacggcc cccccgaccg
     5701 atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg gcgcatgccg
     5761 acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat
     5821 ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc
     5881 agatgtttac cgatgccctt ggaattgacg agtacggtgg gccaaagaag aagaggaagg
     5941 tgtaggcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt
     6001 aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt
     6061 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6121 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6181 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgc caagctaatt
     6241 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6301 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6361 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6421 ttcaataatg ggaccgactc gcgctgtcat gagaccggat cctgacagga tatattggcg
     6481 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     6541 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     6601 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     6661 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     6721 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     6781 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     6841 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     6901 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     6961 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     7021 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     7081 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     7141 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     7201 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     7261 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     7321 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     7381 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     7441 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     7501 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     7561 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc
     7621 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     7681 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     7741 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     7801 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     7861 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     7921 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     7981 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     8041 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     8101 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     8161 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     8221 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     8281 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     8341 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     8401 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     8461 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     8521 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     8581 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     8641 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     8701 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     8761 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg
     8821 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     8881 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     8941 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     9001 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     9061 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     9121 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     9181 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     9241 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     9301 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     9361 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     9421 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     9481 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     9541 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     9601 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     9661 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     9721 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     9781 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     9841 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     9901 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     9961 aaaaggtcga aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta
    10021 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
    10081 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
    10141 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
    10201 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
    10261 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
    10321 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
    10381 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga
    10441 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    10501 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
    10561 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10621 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
    10681 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10741 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11281 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11341 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11401 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11461 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
    11521 gtgattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
    11581 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
    11641 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
    11701 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
    11761 catttcgctc atcaccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
    11821 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
    11881 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
    11941 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
    12001 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
    12061 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
    12121 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
    12181 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
    12241 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
    12301 tcaccgcttc cctcataatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
    12361 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
    12421 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
    12481 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
    12541 acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc
    12601 gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag cgaagtcgag gcatttctgt
    12661 cctggctgga acagaactta ttatttcctt cctcttttct acagtattta aagatacccc
    12721 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
    12781 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
    12841 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
    12901 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
    12961 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
    13021 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    13081 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacataa cggatccggt
    13141 ctca
//
