LOCUS       sequence-460461-       13714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Tol2 based expression vector for RU486 inducible expression of GFP
            in the nucleus with a destablized lex transactivator and a c-fos 
            minimal promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 13714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JD...
FEATURES             Location/Qualifiers
     source          1..13714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4756..4780
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4799..4808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4829..5080
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             6395..6766
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    6933..7054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7073..7164
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7113..7132
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    complement(7684..7708)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7716..7736
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7782..7784,7785..7787,7788..8501)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7782..7784,7785..7787,7788..8498)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(8536..8554)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8537..8554)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8574..8591)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8580..8804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8806..8826)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8834..8850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8866..9000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(9302..10015,10016..10018,10019..10021))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9305..10015,10016..10018,10019..10021))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(11244..11431,11432..11443)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11477..11493)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11477..11493)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11530..11550)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11530..11548)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11569..11585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11582..11604)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11593..11609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11617..11623,11624..11641,11642..11647))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11662..11683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11800..11817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11971..12559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12051..12070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12730..13521,13522..13590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13353..13372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13591..13695)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt ggctccgaat tcgcccttaa aactcgagac cagcaaagtt
     1261 ctagaatttg tcgaaacatt tatgttatat atttcctgaa aaaaattctg agtaagttct
     1321 taagtgttat tgccagcaac ataaacaaca gacggcaaaa tgaataaatg ataacaaagc
     1381 agtaggctta aataaaccta atttttatag gctgttctct acaaccctca aacagtgatt
     1441 agttttgtac ttataaactt gccctttcat tcatatttca agaaaattgg ttcagaagat
     1501 ctggatattc tagcagttgt tcaagctcat ggagggatca gtgacctgat tccacaatga
     1561 ctaggcctaa tccagaaatt agatgactgt caacataaaa aggcacagca ctcactagct
     1621 gccctatata ttttattata ttttacatat attattttat ttatttagct ctgagtgctg
     1681 tactttctgg ttaaagaaaa ctgcttacaa cagctaacct gtactacctc aggctcaggg
     1741 aatttggaac aggtttgtct ggtttgtttc tttaaccatg catgcttgtt ttcaactatg
     1801 gcaacacagt cacatgggac attacagaaa tgatttgtcg atgacatgcg acttttcttt
     1861 aataaagcgc aaagatccca aaaagcaaac ttttaacaaa aatcatataa ttatattttc
     1921 aatccagctt tgtagcaact ttgtgctgct gttcactcag caacagatag tcagtataag
     1981 gtcagtgtgt ctcaaagcag tgccatctgt ttcacacatt gcgttctata tataagtgtg
     2041 ctggttgaca cgacactgta taaggcctag gctaaaacac aaacaatgta gaatgacact
     2101 gtgttttttt tgtaaacaaa tgttgttttt ggttaaacat ctttgtgaaa acatcctcct
     2161 gtcatgtatt tgctatattc aaatgttaaa cccgtgcaga atagaacata tacaaaaaaa
     2221 aacaacacaa cacattttta aacattatta aatatcaagt attgctggca gttctgtttc
     2281 tgttttacag taccctttgc cacagttctc cgcttttcct ggtccagatt ccacaagtct
     2341 gattcaccaa tagcaaagcg aataaacaac caaagcagcc aatcactgct tgtagactgt
     2401 cctgcgagac cggcccattc cagcacattc tggaaacttc ctttatatga taattataaa
     2461 tacatttaaa ttattgatac aaaacatgta attcctagaa cataaccata gcaatcatta
     2521 gttttcaggg taattatgta tttttaggat ttgactgcgg aaagatctgg tcatgtgacg
     2581 tctcatgaac gtcacggccc tgggtttcta taaatacagt aggactctcg accatcggca
     2641 gatttttcga agaagaagat cagtttcagg agccgtactg ttccgtttca acgcaaatat
     2701 taacggtaag agcgaatttc ctagtttgtt ttcatgccat tctttaaaac catagcgtat
     2761 tactttaatt atagtaaact ttcgctttct ttattacaag agacgttttg tgttgattct
     2821 ccgcggacat tttcggtcag acaatcagaa aatgaccgcg gaggaccagt aacttgcatt
     2881 acacgtaagt taaatcttcg tgtattaaaa tggttaggtt gttaacgtca aataggttac
     2941 cgtgtttgcg tgtgatcagg ttggttttgt tagatttttg tcagtatttt taatttattt
     3001 gttttagttt atttattttt tttgctgaat catagtttgt gaacaaagaa cccggatgtt
     3061 acatacagta cagccgccat gttacagaga gttataactt aatcatttta aaaataattt
     3121 tgccttactt ttagtttgtc atgttgagaa atgaggaaat gttaaaatga ggaaatatcc
     3181 aattaattta atatatcaaa ataatccatg attacaatgc actgaactgg agaaaattaa
     3241 gatgttttct agtgtcatga aacaaatgta agagatgtac attgtagatg ttttatgtca
     3301 agaattggct agttgatgca gcatactggc gatactcagt tgtaataaca gtaacgttac
     3361 atgttaatag actactgagt atgctgttct gtctatgtat gctctgtaag ctacgagaag
     3421 gactttttta aacagtaaag ggtgcaatat ttttacaaat tgaattaaat aaaggctgtc
     3481 tattaagtaa tatgcttgat atttttctta cttgatcgga aataagaaaa aatataaacg
     3541 ttgttgctct aaaaatccta gttcagttta gccaaccaca aatacctttt tgttcctcca
     3601 acagtttttt tttcttctct ataatatttg gcagtctata gtactccaaa tgtttcccca
     3661 cagtctaact aattggtaca gccaaaatca tgacacttat tgcaataata attttggttc
     3721 attggcattg ttgatagcct gtgccactaa tatggtcgat tgatcatgct tcaggaagaa
     3781 aactatattg tttgatgtaa gattattaaa tcttcacctg cttccattac aaactattcc
     3841 catcttattg aattctggta tgtcttaaag gattagttca cttcccaaat caaaatttac
     3901 ttagcctttt ttcatccatg atcccttttt ttcatcatta atgaagaaat tgtttttgaa
     3961 aaagtttcaa gatttttttc tctatattgt ggagcttgtt agtttaaaat tccaaaatgc
     4021 aatatgtggc ttcaaatggt tctaaatgat cccagtcaag gaataacagt cttatctaat
     4081 gaaaccatta gacctttaaa aataaaaaaa ataaaagtat ttatttttaa atgactgagt
     4141 gattaagttg aatttcagcg tttccttact gtgtagaagt ccttccttac tggccccacc
     4201 ctttggttct ctgccaatct gctacctaat gtaatgttgt ggaacattat tattctttat
     4261 ttcttaattt tttatttttt attttaaaac aatgtaaact gcacagatgt gcagtttgtt
     4321 taaaatggcc aatgctttgg aaatgcatga cataattaga tttcatgatg cacaaagcca
     4381 aatctcagag cttgtgcaaa atgagctatc atttcactag gtaagaccct aaattttcat
     4441 ataggatcat ttggacaatt ttgctgcagg taaaatgcat tctatagtcc actgtcagcc
     4501 attgttttgg atagtattta tttttctcta caagtatagt caatagtttt ctattatttt
     4561 aaaggtttgt aacatttaag ggtgaccaaa tgcaaagtaa aatttcattt tcgggtgaac
     4621 tatctcgttt aacatgggag aagtgcaaaa catacattat tggctagaac attgtagtat
     4681 tttttaaatg gaaatgtgtg attgctaatc ttactttgaa tttgtttaca gggatccttt
     4741 aagggcgaat tcgacccaag tttgtacaaa aaagcaggct tacgtaacta gtggatccgc
     4801 caccatggca cccaagaaga agaggaagat gaaagcgtta acggccaggc aacaagaggt
     4861 gtttgatctc atccgtgatc acatcagcca gacaggtatg ccgccgacgc gtgcggaaat
     4921 cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa gaacatctga aggcgctggc
     4981 acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga
     5041 agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccgt cgagcgccgg
     5101 taccgaattc ccgggtgtcg accagaaaaa gttcaataaa gtcagagttg tgagagcact
     5161 ggatgctgtt gctctcccac agccagtggg cgttccaaat gaaagccaag ccctaagcca
     5221 gagattcact ttttcaccag gtcaagacat acagttgatt ccaccactga tcaacctgtt
     5281 aatgagcatt gaaccagatg tgatctatgc aggacatgac aacacaaaac ctgacacctc
     5341 cagttctttg ctgacaagtc ttaatcaact aggcgagagg caacttcttt cagtagtcaa
     5401 gtggtctaaa tcattgccag gttttcgaaa cttacatatt gatgaccaga taactctcat
     5461 tcagtattct tggatgagct taatggtgtt tggtctagga tggagatcct acaaacacgt
     5521 cagtgggcag atgctgtatt ttgcacctga tctaatacta aatgaacagc ggatgaaaga
     5581 atcatcattc tattcattat gccttaccat gtggcagatc ccacaggagt ttgtcaagct
     5641 tcaagttagc caagaagagt tcctctgtat gaaagtattg ttacttctta atacaattcc
     5701 tttggaaggg ctacgaagtc aaacccagtt tgaggagatg aggtcaagct acattagaga
     5761 gctcatcaag gcaattggtt tgaggcaaaa aggagttgtg tcgagctcac agcgtttcta
     5821 tcaacttaca aaacttcttg ataacttgca tgatcttgtc aaacaacttc atctgtactg
     5881 cttgaataca tttatccagt cccgggcact gagtgttgaa tttccagaaa tgatgtctga
     5941 agttattgct gggtcgacgc ccatggaatt tcagtacctg ccagatacag acgatcgtca
     6001 ccggattgag gagaaacgta aaaggacata tgagaccttc aagagcatca tgaagaagag
     6061 tcctttcagc ggacccaccg acccccggcc tccacctcga cgcattgctg tgccttcccg
     6121 cagctcagct tctgtcccca agccagcacc ccagccctat ccctttacgt catccctgag
     6181 caccatcaac tatgatgagt ttcccaccat ggtgtttcct tctgggcaga tcagccaggc
     6241 ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc ctgcccctgc
     6301 tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc tagccccagg
     6361 ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg aaggaacgct
     6421 gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct tgcttggcaa
     6481 cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg agtttcagca
     6541 gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc tgatggagta
     6601 ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc cagctcctgc
     6661 tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag acttctcctc
     6721 cattgcggac atggacttct cagccctgct gagtcagatc agctccagat ctcgagatat
     6781 cagccatggc ttcccgccgg cggtggcggc gcaggatgat ggcacgctgc ccatgtcttg
     6841 tgcccaggag agcgggatgg accgtcaccc tgcagcctgt gcttctgcta ggatcaatgt
     6901 gtagtaaatc gatgatcctc cacgtagcgg ccaacttgtt tattgcagct tataatggtt
     6961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7021 gttgtggttt gtccaaactc atcaatgtat cttagatatc gctagcagta ctaacatacg
     7081 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     7141 gtgcaggtgc cagaacattt ctctcagatc tatcgatacg cgtttgcatg ccgcttgggc
     7201 tgcaggtcga ggctaaaaaa ctaatcgcat tatcatcccc tcgacgtact gtacatataa
     7261 ccactggttt tatatacagc agtactgtac atataaccac tggttttata tacagcatct
     7321 agatactgta catataacca ctggttttat atacagcagt actgtacata taaccactgg
     7381 ttttatatac agcagaattc gtactgtaca tataaccact ggttttatat acagcagtac
     7441 tgtacatata accactggtt ttatatacag cagtcgaggt aagattagat atggatatgt
     7501 atatggatat gtatatggtg gtaatgccat gtaatatgct cgactctagg atcttcgtcg
     7561 acggatcccc agtgacgtag gaagtccatc cattcacagc gcttctataa aggcgccagc
     7621 tgaggcgcct actactccaa ccgcgactgc agcgagcaac tctagcaact ctagattaat
     7681 taaacccagc tttcttgtac aaagtggaaa tggctccaaa gaagaagcgt aaggtaaatc
     7741 gatcgaattc aaggcctctc gagcctctag aaggtggcgg aatggtgagc aagggcgagg
     7801 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7861 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7921 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     7981 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8041 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8101 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8161 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     8221 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     8281 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     8341 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     8401 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     8461 ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgct cgagcatgca
     8521 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     8581 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8641 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8701 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8761 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggccaact ttattataca
     8821 tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc agacatgata
     8881 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     8941 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     9001 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     9061 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
     9121 tcttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9241 atcatgtctg gatctacgta atacgactca ctatagttct agaggctcga gaggggccgc
     9301 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     9361 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     9421 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     9481 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     9541 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     9601 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     9661 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     9721 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     9781 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     9841 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     9901 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     9961 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
    10021 tggtcactgt ctgctttgct gttggtctgg gctcctgggt cactggctta ctaatggagt
    10081 ctttatgtat gaggactctt atcaattgtt cttctataaa ggtctgcagt gtttctgttc
    10141 gtcccctaca tggacaccca gagcctccta aatacaggag ccctgataac tgcacaagtg
    10201 ctcagattcc agcagggtgg aaaatgagat aaagtgtgca gatggggagg gggacgtgaa
    10261 tgagagattt gagggatgaa aaggatggat gaacgcattg aaaatagccc ctttcacaaa
    10321 ataataccag taaattgtca tataattaat gaccagacct ttacctgtaa atgatgtgat
    10381 gttcatatac agaagaatgc caacaattta cagatgattt tacaaattct taattttcct
    10441 tttctaaatg gatttgttca cacattatct cttcaaagca attgaatttt ctggaaaaga
    10501 ttggctgtgt gtgtaaaagg tgctactgag gaatttaatg tgacatggaa tgaagcaaac
    10561 agcagcctaa gacaggatgg caggaaaaat gtcacatgtt taaaataaga gtgaaaccaa
    10621 cgacctggac ttatagagac tgtgtgtcct taggcaaagc tcttgtacaa acagtgcttg
    10681 tgactgtgaa attcggggtt tgcctggatt gtgttaaatg tgtgcttgga tgtatcataa
    10741 aaaagaatcg gttctctgta tttgacattt tctgtaattt atctaaagtt acaactggct
    10801 atgcctgatt tttatcacta ttgaatcaat cattttaaag aatgttaagt ctgctgattt
    10861 ggagagctca tttacattta ttttgaatgt ctcttattta acacaactga tttaagcttt
    10921 agatctgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
    10981 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
    11041 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt
    11101 cacttccaaa ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc
    11161 ataggcagaa taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg
    11221 taatagcaag ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa
    11281 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
    11341 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
    11401 gtacagtaat caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc
    11461 ttcagcaagc ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag
    11521 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
    11581 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    11641 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    11701 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11761 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    11821 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    11881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    11941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    12001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    12061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    12121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    12181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    12241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    12301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    12361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    12421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    12481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    12541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    12601 gaacgaaaac tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta
    12661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    12721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    12781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    12841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    12901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    12961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    13021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    13081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    13141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    13201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    13261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    13321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    13381 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    13441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    13501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    13561 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    13621 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    13681 aataggggtt ccgcgcacat ttccccgaaa agtg
//
