LOCUS       sequence-460463-       11715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Zhang J, Fonovic M, Suyama K, Bogyo M, Scott MP
  TITLE     Rab35 controls actin bundling by recruiting fascin as an effector 
            protein.
  JOURNAL   Science. 2009 Sep 4;325(5945):1250-4. doi: 10.1126/science.1174921.
  PUBMED    19729655
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-09-4- 4"; volume: "325"; issue: 
            "5945"; pages: "1250-4"
COMMENT     Sequence Label: pUAST tdTomato fascin WT
FEATURES             Location/Qualifiers
     source          1..11715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    295..389
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        408..646
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     593..614
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             686..2113
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    2177..2201
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3744..3768)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          3861..3926
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4056..4076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4348..4482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4385..4404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4439..4458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4509..4527)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4979..5050,5452..5725,5800..6454,6516..6831,7035..7166,
                     7237..7851)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8630..9184,9185..9215)
                     /label=P element 5' end
     rep_origin      complement(9450..10038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9530..9549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10209..11000,11001..11069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10832..10851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11070..11174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11242..11260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11298..11320)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11420..11439
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcatcca acgcgttggg agctctccgg atccaagctt gcatgcctgc aggtcggagt
      301 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      361 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg gagtataaat
      421 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
      481 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
      541 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
      601 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
      661 aattcgttaa cagatctgcg gccgcatggt gagcaagggc gaggaggtca tcaaagagtt
      721 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
      781 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      841 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
      901 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
      961 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     1021 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     1081 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     1141 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     1201 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     1261 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     1321 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     1381 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     1441 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     1501 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1561 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     1621 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1681 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1741 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     1801 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1861 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     1921 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     1981 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     2041 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     2101 cgagctgtac aagggaagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
     2161 gggcccggga tcatcaacaa gtttgtacaa aaaagcaggc ttaatgaacg gccagggctg
     2221 cgagctgggc cacagcaatg gcgacatcat ctcacagaat caacagaagg gatggtggac
     2281 catcggcctg atcaacggcc agcacaagta catgaccgcg gagacctttg ggttcaagct
     2341 caacgccaac ggcgccagtc tgaagaagaa gcaactgtgg acgctggaac cctcgaacac
     2401 cggtgaaagt attatctact tacgatctca tctgaacaag tacctttcgg tcgatcagtt
     2461 tggcaacgtg ctgtgcgaga gcgatgagag ggacgcgggc agccgcttcc agatcagcat
     2521 cagtgaggac ggcagcggac gttgggcgct gaagaacgag tcgcgcggct actttctggg
     2581 cggcactccg gacaaactgg tctgcacggc caagacgccc ggtgccagtg agttttggac
     2641 ggtccatttg gctgcccggc cgcaggtgaa tctgcgctcc attggacgca agcgattcgc
     2701 ccatttgtcg gagtcgcagg acgagatcca tgtggacgcc aatattcctt ggggcgagga
     2761 tacgctcttt acgctggagt tccgtgccga ggagggcggt cgctatgctc tgcatacgtg
     2821 caacaacaaa tatctgaacg ccaatggaaa attgcaggtg gtgtgcaacg aggattgcct
     2881 gttcagcgcc gaatatcatg gtggccatct ggcgctgcgc gatcgtcagg gtcagtactt
     2941 gtcgcccatt ggctccaagg cggtgttgaa gtcccgctcg tcgtcagtga cgcgggatga
     3001 gctcttctcg ctggaggatt cgctgcctca ggcttccttt atagccggac tcaatttgcg
     3061 atatgtgagc gttaggcagg gcgtcgatgt gacggccaac caggacgagg tcggtgagaa
     3121 cgagacgttc cagctggagt acgattggtc ggcgcaccgt tgggccctac gcaccaccca
     3181 ggatcgctac tggtgtctgt cggcgggcgg tggcatccag gccaccggca atcgtcgctg
     3241 tgccgacgct ctcttcgagc tgatctggca cggcgatggc tcgctctcgt tccgggctaa
     3301 caacggcaag ttcttggcca ccaagcgctc tggtcatctg tttgccacct cggagtcgat
     3361 cgaggagata gccaagttct atttctactt gatcaataga ccaattcttg tactgaagtg
     3421 cgagcaggga ttcgtgggct atcgcacgcc cggtaacctg aagctcgagt gcaataaggc
     3481 cacctacgaa atgattctgg tggagcgggc ccagaagggc ctggtgcatc tgaaggcgca
     3541 tagcggcaaa tactggcgca tcgagggcga aagcatctcg gtggatgccg atgcgcccag
     3601 cgatggtttc ttcctcgagc ttcgcgagcc gaccaggatc tgcataagat cgcagcaggg
     3661 caagtacctg ggcgctacga agaacggggc gttcaagctg ctggatgatg gaaccgattc
     3721 ggccacacag tgggagttct agaacccagc tttcttgtac aaagtggttg atgatcctct
     3781 agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     3841 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     3901 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     3961 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     4021 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     4081 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     4141 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     4201 aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     4261 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4321 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccactagaa ggcctaattc
     4501 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     4561 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     4621 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     4681 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     4741 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     4801 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     4861 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     4921 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     4981 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     5041 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
     5101 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
     5161 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     5221 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     5281 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     5341 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     5401 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
     5461 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
     5521 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
     5581 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
     5641 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
     5701 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
     5761 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
     5821 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
     5881 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
     5941 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
     6001 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc
     6061 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
     6121 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
     6181 gcggagaaag gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga
     6241 tctgcgatga gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc
     6301 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
     6361 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
     6421 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
     6481 atctagtatt acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
     6541 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
     6601 tcccgtgatc ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat
     6661 atggagcagt tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg
     6721 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
     6781 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
     6841 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
     6901 gatagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
     6961 gttttgcaga agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac
     7021 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     7081 cgcaagtggg tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     7141 ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat
     7201 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     7261 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     7321 attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     7381 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     7441 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     7501 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     7561 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     7621 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     7681 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     7741 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     7801 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     7861 atccgaaata actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat
     7921 tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat
     7981 agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa
     8041 ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac
     8101 accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc
     8161 tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac
     8221 attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg
     8281 ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat
     8341 gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta
     8401 atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta
     8461 caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa
     8521 tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat aataagaata
     8581 catttaattt agaaaatgct tggatttcac tggaactaga attaattcgg ctgctgctct
     8641 aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt attttcatta
     8701 aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga ttttttttac
     8761 tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta tcttttctgg
     8821 ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc ttttaaatgt
     8881 ctgacctttt gcaggtgcag ccttccactg cgaatcatta aagtgggtat cacaaatttg
     8941 ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct taatagcaca
     9001 cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt tgcaatattt
     9061 catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa
     9121 cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg taagcttcgg
     9181 ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta gtccagcggc
     9241 agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact ggggcacgtg
     9301 gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg gttaatcagc
     9361 agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag gtatactggc
     9421 accgagcccg agttcaagaa gaaggcgttt ttccataggc tccgcccccc tgacgagcat
     9481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11221 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11281 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11341 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11401 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    11461 tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca
    11521 aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg
    11581 gtgtggtgga ggtgaattat aaaaaaaaaa acaagcagtt atttcggata tatgtcggct
    11641 actccttgcg tcgggcccga agtcttagag ccagatatgc gagcacccgg aagctcacga
    11701 tgagaatggc cagac
//
