LOCUS       sequence-460477-       10081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gateway compatible Tol2 destination vector containing the Hsp70 
            promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)
FEATURES             Location/Qualifiers
     source          1..10081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(667..678,679..841)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1245..1368
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1409..1714)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1511..1530)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2034..2714)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     2629..2648
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2768..2774,2775..2792,2793..2798))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2823..2946)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2954..2970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2990..3071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3595..3729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3686..3705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3881..4579)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     intron          5405..5537
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(5772..5792)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5795..5804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(7669..7856,7857..7868)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7893..7913)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7893..7911)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7932..7948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7932..7948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7945..7967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7956..7972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7980..7986,7987..8004,8005..8010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8025..8046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8163..8180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8334..8922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8414..8433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9093..9884,9885..9953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9716..9735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9954..10058)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 aattaacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      721 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      781 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      841 aagaaaacta gagattcttg tttaagctat ttaatctcaa aaaacattaa atgaaatgca
      901 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      961 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     1021 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     1081 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     1141 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1201 gatcctaggg ggcccgttta aacgccatga ttacgccaag ctatcaactt tgtatagaaa
     1261 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1321 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
     1381 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
     1441 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
     1501 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     1561 ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca
     1621 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     1681 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca
     1741 aaggagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     1801 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg
     1861 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     1921 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     1981 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     2041 gccggatcct ctagattacg ccccgcctgc cactcatcgc agtactgttg taattcatta
     2101 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     2161 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     2221 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2281 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2341 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2401 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2461 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     2521 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2581 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     2641 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2701 atttttttct ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa
     2761 atccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc
     2821 gccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     2881 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact ttattataca
     2941 tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatca acttgtttat
     3001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3061 tttttcactg cgatatcaat cgggacagcc cccccccaaa gcccccaggg atgtaattac
     3121 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     3181 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     3241 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     3301 tacggggaaa aagctttagg ctgagtgcac ggggacagcc cccccccaaa gcccccaggg
     3361 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     3421 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     3481 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     3541 cctgggggga tacggggaaa aagctttagg ctgatacgta gtagatgcgg ccgcaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3721 gtctggatcg cgatcgcgtc tagagtcatt actacacatt gatcctagca gaagcacagg
     3781 ctgcagggtg acggtccatc ccgctctcct gggcacaaga catgggcagc gtgccatcat
     3841 cctgcgccgc caccgccggc gggaagccat gccctcgagc attaagcttg tgccccagtt
     3901 tgctagggag gtcgcagtat ctggccactg ccacctcgtg ctgctcgacg taggtctcgt
     3961 tgttggcctc cttgattctt tccagtctgt agtccacata gtagacgcca ggcatcttga
     4021 ggttcttagc gggtttcttg gatctatatg tggtcttggc gtttgcgatc agatggctcc
     4081 cgcccacgag cttcagggcc atgtcgtttc tgccttccag gccgccgtca gcggggtaca
     4141 gcgtctcggt gaaggcctcc cagccgagtg ttttcttctg catcacaggg ccgttggatg
     4201 tgaagttcac ccctctgatc ttgacgttgt agatgaggca gccgtcctgg aggctggtgt
     4261 cctgggtagc ggtcagcacg cccccgtctt cgtatgtggt gactctctcc catgtgaagc
     4321 cctcagggaa ggactgcttg aagaagtcgg ggatgccctg ggtgtggttg atgaaggtct
     4381 tgctgccgta gaggaagcta gtagccagga tgtcgaaggc gaaggggaga gggccgccct
     4441 cgaccacctt gattctcatg gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg
     4501 tgcacttgaa gtgatggttg tccacggtgc cctccatgta cagcttcatg tgcatgttct
     4561 ccttaatcag ctcgctcatg gtggatccgg ccccgccggc tccgccgcta gcgtctccat
     4621 cctccagcag tctcaccata gctcctgtct cagagtccag gtttctgatg tagttcatca
     4681 caatgttcac atttgtccat cctccagcct gcatgatctc agggatgctc actccagctc
     4741 tagccatgtc tctagcagct cccactctag cagagtgtcc agaccaagcc aggtatctct
     4801 gtccagagtc atccttagct ccatagatca gtctgtgtgt agcctcaaag attccctcca
     4861 gagcccttgt gctcagctgg cttgtagcag aaggagcagc cactccattc tttctcactc
     4921 tacagaacag gtagttgtta gggtcatcag ccactccaga cacagagatc catctctcca
     4981 ccagctttgt cactcccagg ctcagagcct tctccactcc agctgtgctc accagtgtct
     5041 ttgttcttcc aatgtggatc agcattcttc ctccatctgt tctgctgatg tccttcactc
     5101 tgattctagc aatctcagca attctcagca gtgtgttgta agcaattccc aggaaagcca
     5161 ggtttctgat gtcctgacat ctgtcagagt tctccatcag gcttctcacc tggtcaaagt
     5221 ctgttctctc aaaagccaga gcctgcttag ctctctctcc agcatccaca ttctcctttc
     5281 tgattcttct catcaccagg ctcacagcat tgctgtcaga aggtctaggc agtccagacc
     5341 ttctgtgcag catgttcagc tgtcccaggt gctgctggat tgtcttcaca gccagtcctc
     5401 tagcctgtgg agagaaaggc aaagtggatg tcagtaagac caataggtgc ctatcagaaa
     5461 cgcaagagtc ttctctgtct cgacaagccc agtttctatt ggtctcctta aacctgtctt
     5521 gtaaccttga tacttacctg gaggtacagc aggtagtctc tcacatcctc aggctcagca
     5581 gggaaccact ttctgttgtt cagcttacac caagcagccc agctcctgca gacagacagc
     5641 agcatcttcc atgtgtgctc agagaaagcc tgtctgtctc tgaacatgtc catcaggttc
     5701 tttctcacct catcagatgt agcatccaca ggcagagcag gtagattttg gtggacggtt
     5761 agaagattgg agactttcct cttcttcttg ggcaccatgg tggccatatg cggcacgcgt
     5821 caggaaaaaa aaaaacaatt agaattaatt ttatatttat acaatttatc ggtgcaattg
     5881 tttcattatg aaaggatttt tctagtcaac aagtgaaatt ctcttaataa ccaagttatt
     5941 attagttgta caagtaaaac gctaattttc ttttgtagac aacattaaca caaattcatt
     6001 taggacattt gctagtattt atggttatta tgctcatata aaataatgtc agcaacataa
     6061 agtaaatgcc cgtctgttca ttgctttttt taaaatcctt attgtgaaaa cgggaaatat
     6121 aattttctag tagacctaaa ataagatgaa cataggcagt ccacatgtaa aaagattaca
     6181 tatacaaagt tgattttttg acattaagta catataatct ctatataatt ctgattagcc
     6241 aatagagtac aatatattga gacttacctt acagtagatt cttaaataga gcgaataaac
     6301 acctcgtcgg ggaaaagtcc tgtcaggctg ctcttttctt ctttgtcaag tcgcttctct
     6361 tcggttgagt ttctctgcgg tctctctttc gccctttaaa tacagcagag cgcgcacccg
     6421 ttcagactct acactgttct ggaagcctcc agtgaattcg accaatcaaa acacagcagg
     6481 cagccgccgt cacttcttca gctgtcattg agaggagccg ctgagaggaa tgtagtggag
     6541 atttccagag ttttaccgtg aaattacgcg aaacagcggt caaaaataca aagagattta
     6601 aaatgttttt tttatttgag tgttttattg tacttattgc tgaaatatgt gcggttgtgt
     6661 gatgtgttgt ttttccagtt gcaatgaatg aacaaactaa aatgataaaa tgtttgaacg
     6721 attcagtaaa aaaaaatgca ataaaaaaat cttgctgaaa tgttttacac agtagcaaaa
     6781 tcgggcctgt ggtgttttca gctacataga aggcaaacct gttttatata agctaatagc
     6841 tttggtcaga tttgtttaac gtttcaacaa aacaaatgga tgtttactgt actatattga
     6901 ttggttaaaa ccaatacttt attctgggat tgcaggtctt aactattttt gtagtgttaa
     6961 aaacaacttg ctaacttgag aggtcatgtt taccctcaga gaaataggca caagtctctt
     7021 tgtttaatat tggagatcat cctccttgtt cagtcgtggt ctgtatttaa ttttaatgta
     7081 tatgattgcc gtaaaagtgt tagaaagttt accctggtgc tatgaaatca atctgctgct
     7141 tttgacccag ttagtgtgtc tttaatctaa cttaatgaga ccccagtgtg gtcaacaatc
     7201 aatagaaaca aatgtgttgt tttataactg ttaactccta ttatttttta tcttctgtag
     7261 catatagaca aaacatgctc gttctaatag tttaataaaa ttaatttgat ttttggaaat
     7321 aaccaagcga cacccctgaa ctagtatcga tgcgaagata cggccacggg tgctcttgat
     7381 cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca
     7441 ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat
     7501 tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg tctgaccaat
     7561 ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa ttaaactggg
     7621 catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa gtgatctcca
     7681 aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc
     7741 taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt
     7801 aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta
     7861 cacctctgag atctgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     7921 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7981 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8041 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8221 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8281 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8521 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8761 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8941 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9001 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9061 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9121 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9181 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9241 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9301 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9361 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9421 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9481 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9541 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9601 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9661 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9721 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9781 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9841 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9901 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9961 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10021 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10081 c
//
